Restriction map for pF1KB4321
 (Linear) (Six-base) MAPSORT of: KIBB4321.seq  Check: 5257  from: 1  to: 3012



KIBB4321.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1195    2041    3012 
   Size:       1195     846     971

  Fragments arranged by size: 

                1195     971     846
 
AccI GT'mk_AC

Cuts at:      0     683    2947    3012 
   Size:        683    2264      65

  Fragments arranged by size: 

                2264     683      65
 
Acc65I G'GTAC_C

Cuts at:      0    1238    3012 
   Size:       1238    1774
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1583    1635    2848    3012 
   Size:       1583      52    1213     164

  Fragments arranged by size: 

                1583    1213     164      52
 
AflIII A'CryG_T

Cuts at:      0    1163    2792    3012 
   Size:       1163    1629     220

  Fragments arranged by size: 

                1629    1163     220
 
AleI CACnn'nnGTG

Cuts at:      0    1420    1681    3012 
   Size:       1420     261    1331

  Fragments arranged by size: 

                1420    1331     261
 
ApaI G_GGCC'C

Cuts at:      0      17    3012 
   Size:         17    2995
 
ApoI r'AATT_y

Cuts at:      0     183     391    1616    1996    2056    2519    3012 
   Size:        183     208    1225     380      60     463     493

  Fragments arranged by size: 

                1225     493     463     380     208     183      60
 
AvaI C'yCGr_G

Cuts at:      0       9      17    2468    2488    3012 
   Size:          9       8    2451      20     524

  Fragments arranged by size: 

                2451     524      20       9       8
 
AvrII C'CTAG_G

Cuts at:      0    2166    3012 
   Size:       2166     846
 
BamHI G'GATC_C

Cuts at:      0    1974    3012 
   Size:       1974    1038
 
BanI G'GyrC_C

Cuts at:      0     849     898    1238    3012 
   Size:        849      49     340    1774

  Fragments arranged by size: 

                1774     849     340      49
 
BanII G_rGCy'C

Cuts at:      0      10      17     114    1354    1362    3012 
   Size:         10       7      97    1240       8    1650

  Fragments arranged by size: 

                1650    1240      97      10       8       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     717    1602    1641    2836    3012 
   Size:        717     885      39    1195     176

  Fragments arranged by size: 

                1195     885     717     176      39
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2965    3012 
   Size:       2965      47
 
BclI T'GATC_A

Cuts at:      0     477    2196    3012 
   Size:        477    1719     816

  Fragments arranged by size: 

                1719     816     477
 
BfrBI ATG'CAT

Cuts at:      0     866    1908    2845    3012 
   Size:        866    1042     937     167

  Fragments arranged by size: 

                1042     937     866     167
 
BglII A'GATC_T

Cuts at:      0    2400    3012 
   Size:       2400     612
 
Bme1580I G_kGCm'C

Cuts at:      0      17     854    1925    3012 
   Size:         17     837    1071    1087

  Fragments arranged by size: 

                1087    1071     837      17
 
BmgBI CAC'GTC

Cuts at:      0     125    1164    3012 
   Size:        125    1039    1848

  Fragments arranged by size: 

                1848    1039     125
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     439    1675    2649    3012 
   Size:        439    1236     974     363

  Fragments arranged by size: 

                1236     974     439     363
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2295    3012 
   Size:       2295     717
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     443     764    1394    2607    2915    3012 
   Size:        443     321     630    1213     308      97

  Fragments arranged by size: 

                1213     630     443     321     308      97
 
Bpu10I CC'TnA_GC

Cuts at:      0    2003    2996    3012 
   Size:       2003     993      16

  Fragments arranged by size: 

                2003     993      16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1368    3012 
   Size:       1368    1644
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1528    2625    3012 
   Size:       1528    1097     387

  Fragments arranged by size: 

                1528    1097     387
 
BsaAI yAC'GTr

Cuts at:      0    1627    2812    3012 
   Size:       1627    1185     200

  Fragments arranged by size: 

                1627    1185     200
 
BsaBI GATnn'nnATC

Cuts at:      0    1979    3012 
   Size:       1979    1033
 
BsaHI Gr'CG_yC

Cuts at:      0      30    1775    3012 
   Size:         30    1745    1237

  Fragments arranged by size: 

                1745    1237      30
 
BsaWI w'CCGG_w

Cuts at:      0     149    3012 
   Size:        149    2863
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1070    1231    1771    3012 
   Size:       1070     161     540    1241

  Fragments arranged by size: 

                1241    1070     540     161
 
BseYI C'CCAG_C

Cuts at:      0      72     169    3012 
   Size:         72      97    2843

  Fragments arranged by size: 

                2843      97      72
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     933    3012 
   Size:         83     850    2079

  Fragments arranged by size: 

                2079     850      83
 
BsiHKAI G_wGCw'C

Cuts at:      0    1626    3012 
   Size:       1626    1386
 
BsmI GAATG_Cn'

Cuts at:      0    1092    3012 
   Size:       1092    1920
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      17     114     854    1354    1362    1626    1925 
   Size:         10       7      97     740     500       8     264     299

Cuts at:   1925    3012 
   Size:       1087

  Fragments arranged by size: 

                1087     740     500     299     264      97      10       8
                   7
 
BspEI T'CCGG_A

Cuts at:      0     149    3012 
   Size:        149    2863
 
BspHI T'CATG_A

Cuts at:      0    1277    3012 
   Size:       1277    1735
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1195    2041    3012 
   Size:       1195     846     971

  Fragments arranged by size: 

                1195     971     846
 
BsrDI GCAATG_nn'

Cuts at:      0     180     527     659    3012 
   Size:        180     347     132    2353

  Fragments arranged by size: 

                2353     347     180     132
 
BsrFI r'CCGG_y

Cuts at:      0      32      85    2942    3012 
   Size:         32      53    2857      70

  Fragments arranged by size: 

                2857      70      53      32
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1190    3012 
   Size:       1190    1822
 
BstEII G'GTnAC_C

Cuts at:      0     565    2229    3000    3012 
   Size:        565    1664     771      12

  Fragments arranged by size: 

                1664     771     565      12
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2531    3012 
   Size:       2531     481
 
BstYI r'GATC_y

Cuts at:      0     350    1287    1887    1974    2400    2890    3012 
   Size:        350     937     600      87     426     490     122

  Fragments arranged by size: 

                 937     600     490     426     350     122      87
 
BstZ17I GTA'TAC

Cuts at:      0     684    2948    3012 
   Size:        684    2264      64

  Fragments arranged by size: 

                2264     684      64
 
BtgI C'CryG_G

Cuts at:      0    2702    3012 
   Size:       2702     310
 
BtsI GCAGTG_nn'

Cuts at:      0     822    1334    1769    3012 
   Size:        822     512     435    1243

  Fragments arranged by size: 

                1243     822     512     435
 
EaeI y'GGCC_r

Cuts at:      0      83     161    3012 
   Size:         83      78    2851

  Fragments arranged by size: 

                2851      83      78
 
EarI CTCTTCn'nnn_

Cuts at:      0     245     881    1889    3012 
   Size:        245     636    1008    1123

  Fragments arranged by size: 

                1123    1008     636     245
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1124    3012 
   Size:       1124    1888
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     443     764    1394    1583    1635    2607    2848    2915 
   Size:        443     321     630     189      52     972     241      67

Cuts at:   2915    3012 
   Size:         97

  Fragments arranged by size: 

                 972     630     443     321     241     189      97      67
                  52
 
EcoO109I rG'GnC_Cy

Cuts at:      0      13      14     213     434     607    3012 
   Size:         13       1     199     221     173    2405

  Fragments arranged by size: 

                2405     221     199     173      13       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     465    3012 
   Size:        465    2547
 
HaeII r_GCGC'y

Cuts at:      0     833    3012 
   Size:        833    2179
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1272    1304    2637    2669    3012 
   Size:       1272      32    1333      32     343

  Fragments arranged by size: 

                1333    1272     343      32      32
 
HincII GTy'rAC

Cuts at:      0     789    2790    3012 
   Size:        789    2001     222

  Fragments arranged by size: 

                2001     789     222
 
HindIII A'AGCT_T

Cuts at:      0     543     992    3012 
   Size:        543     449    2020

  Fragments arranged by size: 

                2020     543     449
 
KpnI G_GTAC'C

Cuts at:      0    1242    3012 
   Size:       1242    1770
 
MfeI C'AATT_G

Cuts at:      0     760    3012 
   Size:        760    2252
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     328    1367    2777    3012 
   Size:        328    1039    1410     235

  Fragments arranged by size: 

                1410    1039     328     235
 
MscI TGG'CCA

Cuts at:      0     163    3012 
   Size:        163    2849
 
MslI CAynn'nnrTG

Cuts at:      0     205    1205    1282    1420    1681    1967    3012 
   Size:        205    1000      77     138     261     286    1045

  Fragments arranged by size: 

                1045    1000     286     261     205     138      77
 
NaeI GCC'GGC

Cuts at:      0      34      87    3012 
   Size:         34      53    2925

  Fragments arranged by size: 

                2925      53      34
 
NcoI C'CATG_G

Cuts at:      0    2702    3012 
   Size:       2702     310
 
NgoMIV G'CCGG_C

Cuts at:      0      32      85    3012 
   Size:         32      53    2927

  Fragments arranged by size: 

                2927      53      32
 
NsiI A_TGCA'T

Cuts at:      0     868    1910    2847    3012 
   Size:        868    1042     937     165

  Fragments arranged by size: 

                1042     937     868     165
 
NspI r_CATG'y

Cuts at:      0     870    1061    1184    2343    2796    3012 
   Size:        870     191     123    1159     453     216

  Fragments arranged by size: 

                1159     870     453     216     191     123
 
PciI A'CATG_T

Cuts at:      0    2792    3012 
   Size:       2792     220
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     241    3012 
   Size:        241    2771
 
PfoI T'CCnGG_A

Cuts at:      0     420    3012 
   Size:        420    2592
 
PpuMI rG'GwC_Cy

Cuts at:      0     213     434     607    3012 
   Size:        213     221     173    2405

  Fragments arranged by size: 

                2405     221     213     173
 
PspOMI G'GGCC_C

Cuts at:      0      13    3012 
   Size:         13    2999
 
PstI C_TGCA'G

Cuts at:      0     829    1581    2361    2854    3012 
   Size:        829     752     780     493     158

  Fragments arranged by size: 

                 829     780     752     493     158
 
SfcI C'TryA_G

Cuts at:      0     825    1577    1869    2357    2850    2901    3012 
   Size:        825     752     292     488     493      51     111

  Fragments arranged by size: 

                 825     752     493     488     292     111      51
 
SgrAI Cr'CCGG_yG

Cuts at:      0      32    3012 
   Size:         32    2980
 
SmaI CCC'GGG

Cuts at:      0      19    2470    3012 
   Size:         19    2451     542

  Fragments arranged by size: 

                2451     542      19
 
SmlI C'TyrA_G

Cuts at:      0    1347    3012 
   Size:       1347    1665
 
SnaBI TAC'GTA

Cuts at:      0    2812    3012 
   Size:       2812     200
 
SpeI A'CTAG_T

Cuts at:      0    1111    3012 
   Size:       1111    1901
 
SphI G_CATG'C

Cuts at:      0    1061    1184    2343    3012 
   Size:       1061     123    1159     669

  Fragments arranged by size: 

                1159    1061     669     123
 
StyI C'CwwG_G

Cuts at:      0    2166    2702    3005    3012 
   Size:       2166     536     303       7

  Fragments arranged by size: 

                2166     536     303       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     233     817    1062    3012 
   Size:        233     584     245    1950

  Fragments arranged by size: 

                1950     584     245     233
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     929     961    3012 
   Size:        929      32    2051

  Fragments arranged by size: 

                2051     929      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     242    1853    3012 
   Size:        242    1611    1159

  Fragments arranged by size: 

                1611    1159     242
 
XmaI C'CCGG_G

Cuts at:      0      17    2468    3012 
   Size:         17    2451     544

  Fragments arranged by size: 

                2451     544      17
 
XmnI GAAnn'nnTTC

Cuts at:      0    1130    3012 
   Size:       1130    1882
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AleI     ApaI     ApoI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BciVI     BclI
    BfrBI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrDI
    BsrFI   BstAPI   BstEII    BstXI    BstYI  BstZ17I     BtgI     BtsI
     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
   HincII  HindIII     KpnI     MfeI     MmeI     MscI     MslI     NaeI
     NcoI   NgoMIV     NsiI     NspI     PciI    PflMI     PfoI    PpuMI
   PspOMI     PstI     SfcI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     StyI    TaqII     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI     BaeI     BbeI    BbvCI
     BcgI     BglI     BlpI     BmtI    BsaXI    BsiEI    BsiWI    BsmBI
    BsrBI    BsrGI   BssHII    BssSI    BstBI   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI     HpaI     KasI     MluI   MspA1I
     NarI     NdeI     NheI     NotI     NruI     PacI     PasI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XhoI     ZraI
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