Restriction map for pF1KB4139
 (Linear) (Six-base) MAPSORT of: KIBB4139.seq  Check: 9047  from: 1  to: 1308



KIBB4139.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     668    1308 
   Size:        668     640
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204    1119    1308 
   Size:        204     915     189

  Fragments arranged by size: 

                 915     204     189
 
AfeI AGC'GCT

Cuts at:      0      45    1308 
   Size:         45    1263
 
AleI CACnn'nnGTG

Cuts at:      0     506    1010    1308 
   Size:        506     504     298

  Fragments arranged by size: 

                 506     504     298
 
ApoI r'AATT_y

Cuts at:      0       7     169     413     497     749     833    1001    1085 
   Size:          7     162     244      84     252      84     168      84

Cuts at:   1085    1169    1308 
   Size:         84     139

  Fragments arranged by size: 

                 252     244     168     162     139      84      84      84
                  84       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     967    1308 
   Size:        967     341
 
BfrBI ATG'CAT

Cuts at:      0    1031    1308 
   Size:       1031     277
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     207    1308 
   Size:        207    1101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     695    1308 
   Size:        695     613
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     795    1308 
   Size:        149     646     513

  Fragments arranged by size: 

                 646     513     149
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     106    1270    1308 
   Size:        106    1164      38

  Fragments arranged by size: 

                1164     106      38
 
BsmI GAATG_Cn'

Cuts at:      0     292    1033    1308 
   Size:        292     741     275

  Fragments arranged by size: 

                 741     292     275
 
BspHI T'CATG_A

Cuts at:      0     864    1023    1308 
   Size:        864     159     285

  Fragments arranged by size: 

                 864     285     159
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     668    1308 
   Size:        668     640
 
BsrDI GCAATG_nn'

Cuts at:      0     360     387     622    1308 
   Size:        360      27     235     686

  Fragments arranged by size: 

                 686     360     235      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     706    1308 
   Size:        706     602
 
DraI TTT'AAA

Cuts at:      0      12     321    1308 
   Size:         12     309     987

  Fragments arranged by size: 

                 987     309      12
 
EarI CTCTTCn'nnn_

Cuts at:      0    1250    1308 
   Size:       1250      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     695    1119    1308 
   Size:        204     491     424     189

  Fragments arranged by size: 

                 491     424     204     189
 
EcoRI G'AATT_C

Cuts at:      0     413     497     749    1001    1085    1169    1308 
   Size:        413      84     252     252      84      84     139

  Fragments arranged by size: 

                 413     252     252     139      84      84      84
 
HaeII r_GCGC'y

Cuts at:      0      47    1308 
   Size:         47    1261
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     173     205    1308 
   Size:        173      32    1103

  Fragments arranged by size: 

                1103     173      32
 
HindIII A'AGCT_T

Cuts at:      0     813    1149    1308 
   Size:        813     336     159

  Fragments arranged by size: 

                 813     336     159
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    1308 
   Size:        352     956
 
MslI CAynn'nnrTG

Cuts at:      0     422     506     590     758     869     926    1010    1094 
   Size:        422      84      84     168     111      57      84      84

Cuts at:   1094    1308 
   Size:        214

  Fragments arranged by size: 

                 422     214     168     111      84      84      84      84
                  57
 
NsiI A_TGCA'T

Cuts at:      0    1033    1308 
   Size:       1033     275
 
NspI r_CATG'y

Cuts at:      0     135    1308 
   Size:        135    1173
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     470     502    1308 
   Size:        470      32     806

  Fragments arranged by size: 

                 806     470      32
 
SmlI C'TyrA_G

Cuts at:      0     164     810    1308 
   Size:        164     646     498

  Fragments arranged by size: 

                 646     498     164
 
StuI AGG'CCT

Cuts at:      0     379     460     544     796    1048    1132    1308 
   Size:        379      81      84     252     252      84     176

  Fragments arranged by size: 

                 379     252     252     176      84      84      81
 
XmnI GAAnn'nnTTC

Cuts at:      0     258    1132    1173    1308 
   Size:        258     874      41     135

  Fragments arranged by size: 

                 874     258     135      41
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AleI     ApoI     BbsI    BfrBI     BplI
     BpmI    BpuEI    BseRI     BsmI    BspHI    BspMI    BsrDI    BtgZI
     DraI     EarI  Eco57MI    EcoRI    HaeII    Hin4I  HindIII     MmeI
     MslI     NsiI     NspI     PsrI     SmlI     StuI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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