Restriction map for pF1KB3870
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        HincII      HindII      
HpaI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tsp509I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          265  GTCTAC               GT^[AC][GT]AC
AccII         511  CGCG                 CG^CG
AflIII        653  ACATGT               A^C[GA][CT]GT
AlwNI         811  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          933  CCTGAGG              CC^T[ACGT]AGG
AspI          723  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           61  CCCGGG               C^[CT]CG[GA]G
BbsI         1032  GTCTTC               GAAGAC(2/6)
BciVI           3  GTATCC               GTATCC(6/5)
BglI          274  GCCGGCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         353  AGATCT               A^GATCT
BpuAI        1032  GTCTTC               GAAGAC(2/6)
BsaAI         451  TACGTG               [CT]AC^GT[GA]
BseRI         838  GAGGAG               GAGGAG(10/8)
BsmBI         376  GAGACG               CGTCTC(1/5)
BsoBI          61  CCCGGG               C^[CT]CG[GA]G
BspLU11I      653  ACATGT               A^CATGT
BsrBI         382  GAGCGG               CCGCTC(-3/-3)
BssSI        1045  CACGAG               CACGAG(-5/-1)
BstEII        171  GGTGACC              G^GT[ACGT]ACC
BstPI         171  GGTGACC              G^GT[ACGT]ACC
BstUI         511  CGCG                 CG^CG
Bsu36I        933  CCTGAGG              CC^T[ACGT]AGG
Eco81I        933  CCTGAGG              CC^T[ACGT]AGG
EcoNI         831  CCTCAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       171  GGTGACC              G^GT[ACGT]ACC
HindIII       422  AAGCTT               A^AGCTT
MseI          308  TTAA                 T^TAA
MslI          489  CACCCCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          511  CGCG                 CG^CG
NcoI          575  CCATGG               C^CATGG
NspI          653  ACATGT               [GA]CATG^[CT]
PflFI         723  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1013  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         519  GACCCACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          570  GAGCTC               GAGCT^C
ScaI           76  AGTACT               AGT^ACT
SexAI         724  ACCTGGT              A^CC[AT]GGT
SmaI           61  CCCGGG               CCC^GGG
SmlI          786  CTCAAG               C^T[CT][GA]AG
StuI          590  AGGCCT               AGG^CCT
TfiI           10  GAATC                G^A[AT]TC
Tru9I         308  TTAA                 T^TAA
Tth111I       723  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          61  CCCGGG               C^CCGGG
XmaI           61  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI          715  GGCGCC               G[GA]^CG[CT]C
              848  GGCGCC              
AlwI          828  GATCC                GGATC(4/5)
             1097  GATCC               
AspHI         570  GAGCTC               G[AT]GC[AT]^C
              958  GTGCTC              
BalI          542  TGGCCA               TGG^CCA
              917  TGGCCA              
BbeI          715  GGCGCC               GGCGC^C
              848  GGCGCC              
BsaHI         715  GGCGCC               G[GA]^CG[CT]C
              848  GGCGCC              
BsaI          502  GAGACC               GGTCTC(1/5)
              144  GGTCTC              
BsgI          900  CTGCAC               GTGCAG(16/14)
              679  GTGCAG              
BsiHKAI       570  GAGCTC               G[AT]GC[AT]^C
              958  GTGCTC              
BsrFI         274  GCCGGC               [GA]^CCGG[CT]
              845  GCCGGC              
Cfr10I        274  GCCGGC               [GA]^CCGG[CT]
              845  GCCGGC              
DsaI          575  CCATGG               C^C[GA][CT]GG
              599  CCGTGG              
EaeI          542  TGGCCA               [CT]^GGCC[GA]
              917  TGGCCA              
EarI          247  CTCTTC               CTCTTC(1/4)
              499  GAAGAG              
EcoT14I       221  CCTTGG               C^C[AT][AT]GG
              575  CCATGG              
Hin1I         715  GGCGCC               G[GA]^CG[CT]C
              848  GGCGCC              
KasI          715  GGCGCC               G^GCGCC
              848  GGCGCC              
Ksp632I       247  CTCTTC               CTCTTC(1/4)
              499  GAAGAG              
MaeIII        172  GTGAC                ^GT[ACGT]AC
              561  GTCAC               
MluNI         542  TGGCCA               TGG^CCA
              917  TGGCCA              
MscI          542  TGGCCA               TGG^CCA
              917  TGGCCA              
NaeI          274  GCCGGC               GCC^GGC
              845  GCCGGC              
NarI          715  GGCGCC               GG^CGCC
              848  GGCGCC              
NgoMIV        274  GCCGGC               G^CCGGC
              845  GCCGGC              
PleI           90  GAGTC                GAGTC(4/5)
              947  GAGTC               
PstI          439  CTGCAG               CTGCA^G
              874  CTGCAG              
SfaNI         239  GCATC                GCATC(5/9)
              773  GCATC               
SfoI          715  GGCGCC               GGC^GCC
              848  GGCGCC              
StyI          221  CCTTGG               C^C[AT][AT]GG
              575  CCATGG              
TaqI          251  TCGA                 T^CGA
              410  TCGA                
Tsp45I        172  GTGAC                ^GT[GC]AC
              561  GTCAC               
TthHB8I       251  TCGA                 T^CGA
              410  TCGA                
XcmI          485  CCAACACCCCGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              575  CCATGGAGATCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        TseI        TspRI       Van91I      XhoII       
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