Restriction map for pF1KB3863
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       BamHI       
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BsaBI       BsaHI       BsaI        
BseAI       BsiEI       BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstEII      
BstPI       BstUI       BstXI       BstYI       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


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# ONE CUT ENZYMES (   32 enzymes) #
###################################

AocI          922  CCTAAGG              CC^T[ACGT]AGG
ApaI           66  GGGCCC               GGGCC^C
AvrII        1726  CCTAGG               C^CTAGG
BbvCI        1185  CCTCAGC              CCTCAGC(-5/-2)
BlnI         1726  CCTAGG               C^CTAGG
BsaWI         330  ACCGGA               [AT]^CCGG[AT]
BsmBI         651  CGTCTC               CGTCTC(1/5)
BsmFI        1611  GTCCC                GGGAC(10/14)
BsmI           45  GAATGC               GAATGC(1/-1)
BspMI        1872  ACCTGC               ACCTGC(4/8)
BsrBI         778  CCGCTC               CCGCTC(-3/-3)
Bst1107I      678  GTATAC               GTA^TAC
BstBI          98  TTCGAA               TT^CGAA
BstZ17I       678  GTATAC               GTA^TAC
Bsu36I        922  CCTAAGG              CC^T[ACGT]AGG
DpnII        1294  GATC                 ^GATC
DsaI         1178  CCATGG               C^C[GA][CT]GG
Eco81I        922  CCTAAGG              CC^T[ACGT]AGG
HindIII      1033  AAGCTT               A^AGCTT
HphI          446  TCACC                GGTGA(8/7)
MboI         1294  GATC                 ^GATC
MslI         1054  CATTGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1178  CCATGG               C^CATGG
NdeII        1294  GATC                 ^GATC
NheI          898  GCTAGC               G^CTAGC
NspI         1092  GCATGC               [GA]CATG^[CT]
NspV           98  TTCGAA               TT^CGAA
PstI          794  CTGCAG               CTGCA^G
Sau3AI       1294  GATC                 ^GATC
SfuI           98  TTCGAA               TT^CGAA
SnaBI        2120  TACGTA               TAC^GTA
SphI         1092  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          326  GTCTAC               GT^[AC][GT]AC
              678  GTATAC              
Asp700I      1031  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1573  GAACAGCTTC          
AspHI        1017  GAGCTC               G[AT]GC[AT]^C
             1464  GAGCTC              
BalI          289  TGGCCA               TGG^CCA
             1418  TGGCCA              
BbsI         1428  GTCTTC               GAAGAC(2/6)
             2373  GTCTTC              
BlpI         1556  GCTTAGC              GC^T[ACGT]AGC
             1947  GCTGAGC             
Bpu1102I     1556  GCTTAGC              GC^T[ACGT]AGC
             1947  GCTGAGC             
BpuAI        1428  GTCTTC               GAAGAC(2/6)
             2373  GTCTTC              
BsaAI        2027  TACGTG               [CT]AC^GT[GA]
             2120  TACGTA              
BsgI         1136  CTGCAC               GTGCAG(16/14)
             2294  CTGCAC              
BsiHKAI      1017  GAGCTC               G[AT]GC[AT]^C
             1464  GAGCTC              
CelII        1556  GCTTAGC              GC^T[ACGT]AGC
             1947  GCTGAGC             
EaeI          289  TGGCCA               [CT]^GGCC[GA]
             1418  TGGCCA              
EcoT14I      1178  CCATGG               C^C[AT][AT]GG
             1726  CCTAGG              
MluNI         289  TGGCCA               TGG^CCA
             1418  TGGCCA              
MscI          289  TGGCCA               TGG^CCA
             1418  TGGCCA              
PvuII         232  CAGCTG               CAG^CTG
             1945  CAGCTG              
SacI         1017  GAGCTC               GAGCT^C
             1464  GAGCTC              
StyI         1178  CCATGG               C^C[AT][AT]GG
             1726  CCTAGG              
TaqI           99  TCGA                 T^CGA
             1689  TCGA                
TthHB8I        99  TCGA                 T^CGA
             1689  TCGA                
XmnI         1031  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1573  GAACAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        Aor51HI     AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       EarI        
Eco47III    EcoNI       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboII       
MnlI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PleI        
RsaI        Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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