Restriction map for pF1KB3831
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AgeI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AvrII       BbrPI       
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaI        BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        DraI        EarI        
EcoRI       EcoRV       EcoT22I     FbaI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
MfeI        MluI        MroI        MseI        MunI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        SalI        
SapI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         214  GACGTC               GACGT^C
AflIII        779  ACGTGT               A^C[GA][CT]GT
AhdI          214  GACGTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2038  GTGCAC               G^TGCAC
Aor51HI       938  AGCGCT               AGC^GCT
ApaLI        2038  GTGCAC               G^TGCAC
AspEI         214  GACGTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          787  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1361  TGCGCA               TGC^GCA
BamHI         704  GGATCC               G^GATCC
BbeI          763  GGCGCC               GGCGC^C
BbsI         1212  GAAGAC               GAAGAC(2/6)
BbvCI        2011  GCTGAGG              CCTCAGC(-5/-2)
BcgI          636  CGATGGCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI        1212  GAAGAC               GAAGAC(2/6)
BsaBI        2092  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1735  GAGACG               CGTCTC(1/5)
BsrDI         272  CATTGC               GCAATG(2/0)
BsrI         1222  ACTGG                ACTGG(1/-1)
BstEII        792  GGTCACC              G^GT[ACGT]ACC
BstPI         792  GGTCACC              G^GT[ACGT]ACC
BstYI         704  GGATCC               [GA]^GATC[CT]
CpoI           47  CGGTCCG              CG^G[AT]CCG
DpnII         705  GATC                 ^GATC
Eam1105I      214  GACGTCATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      938  AGCGCT               AGC^GCT
EcoO65I       792  GGTCACC              G^GT[ACGT]ACC
EcoT14I       647  CCATGG               C^C[AT][AT]GG
FseI         1503  GGCCGGCC             GGCCGG^CC
FspI         1361  TGCGCA               TGC^GCA
HinfI           7  GAGTC                G^A[ACGT]TC
KasI          763  GGCGCC               G^GCGCC
MamI         2092  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          705  GATC                 ^GATC
MflI          704  GGATCC               [GA]^GATC[CT]
NarI          763  GGCGCC               GG^CGCC
NcoI          647  CCATGG               C^CATGG
NdeII         705  GATC                 ^GATC
NspI          357  GCATGT               [GA]CATG^[CT]
PaeR7I        948  CTCGAG               C^TCGAG
PflFI         787  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI            7  GAGTC                GAGTC(4/5)
PshAI        1737  GACGGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII          47  CGGTCCG              CG^G[AT]CCG
Sau3AI        705  GATC                 ^GATC
SexAI         572  ACCAGGT              A^CC[AT]GGT
SfoI          763  GGCGCC               GGC^GCC
SmlI          948  CTCGAG               C^T[CT][GA]AG
StyI          647  CCATGG               C^C[AT][AT]GG
Tth111I       787  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          948  CTCGAG               C^TCGAG
XhoII         704  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwI          705  GATCC                GGATC(4/5)
              704  GGATC               
AocI          861  CCTGAGG              CC^T[ACGT]AGG
              988  CCTCAGG             
BalI          902  TGGCCA               TGG^CCA
             1196  TGGCCA              
BlpI          747  GCTGAGC              GC^T[ACGT]AGC
             1494  GCTCAGC             
BpmI          421  CTCCAG               CTGGAG(16/14)
             2080  CTGGAG              
Bpu1102I      747  GCTGAGC              GC^T[ACGT]AGC
             1494  GCTCAGC             
BsiEI        1506  CGGCCG               CG[GA][CT]^CG
             1817  CGGCCG              
BstXI        1067  CCATCTCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1199  CCAAGAGCCTGG        
Bsu36I        861  CCTGAGG              CC^T[ACGT]AGG
              988  CCTCAGG             
CelII         747  GCTGAGC              GC^T[ACGT]AGC
             1494  GCTCAGC             
DraIII        801  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1828  CACACAGTG           
DrdI         1233  GACACAGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1408  GACGCGCTGGTC        
EagI         1506  CGGCCG               C^GGCCG
             1817  CGGCCG              
EclXI        1506  CGGCCG               C^GGCCG
             1817  CGGCCG              
Eco52I       1506  CGGCCG               C^GGCCG
             1817  CGGCCG              
Eco81I        861  CCTGAGG              CC^T[ACGT]AGG
              988  CCTCAGG             
EcoNI        1359  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1986  CCTCGGGGAGG         
MluNI         902  TGGCCA               TGG^CCA
             1196  TGGCCA              
MscI          902  TGGCCA               TGG^CCA
             1196  TGGCCA              
NheI          822  GCTAGC               G^CTAGC
             1935  GCTAGC              
SacI          418  GAGCTC               GAGCT^C
              835  GAGCTC              
SmaI           71  CCCGGG               CCC^GGG
             1999  CCCGGG              
XmaCI          71  CCCGGG               C^CCGGG
             1999  CCCGGG              
XmaI           71  CCCGGG               C^CCGGG
             1999  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        KspI        
MaeI        MaeII       MaeIII      MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NgoMIV      NlaIII      
NlaIV       PflMI       PpuMI       PstI        PvuII       
RsaI        SacII       Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com