Restriction map for pF1KB3831
 (Linear) (Six-base) MAPSORT of: KIBB3831.seq  Check: 1640  from: 1  to: 2103



KIBB3831.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     393     698    2103 
   Size:        393     305    1405

  Fragments arranged by size: 

                1405     393     305
 
AatII G_ACGT'C

Cuts at:      0     218    2103 
   Size:        218    1885
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     360    2103 
   Size:        112     248    1743

  Fragments arranged by size: 

                1743     248     112
 
AfeI AGC'GCT

Cuts at:      0     940    2103 
   Size:        940    1163
 
AflIII A'CryG_T

Cuts at:      0     779    2103 
   Size:        779    1324
 
AhdI GACnn_n'nnGTC

Cuts at:      0     219    2103 
   Size:        219    1884
 
AleI CACnn'nnGTG

Cuts at:      0     910    1264    2103 
   Size:        910     354     839

  Fragments arranged by size: 

                 910     839     354
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1549    1945    2103 
   Size:       1549     396     158

  Fragments arranged by size: 

                1549     396     158
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2011    2043    2103 
   Size:       2011      32      60

  Fragments arranged by size: 

                2011      60      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     630    1128    1222    1896    2103 
   Size:        156     474     498      94     674     207

  Fragments arranged by size: 

                 674     498     474     207     156      94
 
ApaI G_GGCC'C

Cuts at:      0     725    1359    1783    1917    1999    2103 
   Size:        725     634     424     134      82     104

  Fragments arranged by size: 

                 725     634     424     134     104      82
 
ApaLI G'TGCA_C

Cuts at:      0    2038    2103 
   Size:       2038      65
 
AvaI C'yCGr_G

Cuts at:      0      71     948    1987    1999    2103 
   Size:         71     877    1039      12     104

  Fragments arranged by size: 

                1039     877     104      71      12
 
BamHI G'GATC_C

Cuts at:      0     704    2103 
   Size:        704    1399
 
BanI G'GyrC_C

Cuts at:      0      42     249     346     713     763     867    1290    1426 
   Size:         42     207      97     367      50     104     423     136

Cuts at:   1426    1806    2103 
   Size:        380     297

  Fragments arranged by size: 

                 423     380     367     297     207     136     104      97
                  50      42
 
BanII G_rGCy'C

Cuts at:      0     422     725     839    1004    1359    1402    1604    1732 
   Size:        422     303     114     165     355      43     202     128

Cuts at:   1732    1783    1917    1999    2067    2103 
   Size:         51     134      82      68      36

  Fragments arranged by size: 

                 422     355     303     202     165     134     128     114
                  82      68      51      43      36
 
BbeI G_GCGC'C

Cuts at:      0     767    2103 
   Size:        767    1336
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1219    2103 
   Size:       1219     884
 
BbvCI CC'TCA_GC

Cuts at:      0    2012    2103 
   Size:       2012      91
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     625     659    2103 
   Size:        625      34    1444

  Fragments arranged by size: 

                1444     625      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     652     772    1051    1195    1505    1537    2103 
   Size:        652     120     279     144     310      32     566

  Fragments arranged by size: 

                 652     566     310     279     144     120      32
 
BlpI GC'TnA_GC

Cuts at:      0     748    1495    2103 
   Size:        748     747     608

  Fragments arranged by size: 

                 748     747     608
 
Bme1580I G_kGCm'C

Cuts at:      0     349     725    1359    1783    1811    1917    1999    2042 
   Size:        349     376     634     424      28     106      82      43

Cuts at:   2042    2103 
   Size:         61

  Fragments arranged by size: 

                 634     424     376     349     106      82      61      43
                  28
 
BmgBI CAC'GTC

Cuts at:      0     789    2103 
   Size:        789    1314
 
BmtI G_CTAG'C

Cuts at:      0     826    1939    2103 
   Size:        826    1113     164

  Fragments arranged by size: 

                1113     826     164
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1746    2002    2103 
   Size:       1746     256     101

  Fragments arranged by size: 

                1746     256     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406    2101    2103 
   Size:        406    1695       2

  Fragments arranged by size: 

                1695     406       2
 
Bpu10I CC'TnA_GC

Cuts at:      0      14    1598    2012    2103 
   Size:         14    1584     414      91

  Fragments arranged by size: 

                1584     414      91      14
 
BsaBI GATnn'nnATC

Cuts at:      0    2096    2103 
   Size:       2096       7
 
BsaHI Gr'CG_yC

Cuts at:      0     215     587     764    1478    2103 
   Size:        215     372     177     714     625

  Fragments arranged by size: 

                 714     625     372     215     177
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2011    2041    2103 
   Size:       2011      30      62

  Fragments arranged by size: 

                2011      62      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1991    2029    2032    2103 
   Size:       1991      38       3      71

  Fragments arranged by size: 

                1991      71      38       3
 
BseYI C'CCAG_C

Cuts at:      0      63     597     693    2103 
   Size:         63     534      96    1410

  Fragments arranged by size: 

                1410     534      96      63
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     141     169    2071    2103 
   Size:        129      12      28    1902      32

  Fragments arranged by size: 

                1902     129      32      28      12
 
BsiEI CG_ry'CG

Cuts at:      0    1509    1820    2103 
   Size:       1509     311     283

  Fragments arranged by size: 

                1509     311     283
 
BsiHKAI G_wGCw'C

Cuts at:      0     422     839    1885    2042    2103 
   Size:        422     417    1046     157      61

  Fragments arranged by size: 

                1046     422     417     157      61
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1729    2103 
   Size:       1729     374
 
Bsp1286I G_dGCh'C

Cuts at:      0     349     422     725     839    1004    1359    1402    1604 
   Size:        349      73     303     114     165     355      43     202

Cuts at:   1604    1732    1783    1811    1885    1917    1999    2042    2067 
   Size:        128      51      28      74      32      82      43      25

Cuts at:   2067    2103 
   Size:         36

  Fragments arranged by size: 

                 355     349     303     202     165     128     114      82
                  74      73      51      43      43      36      32      28
                  25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     164     393     698    1904    2103 
   Size:        164     229     305    1206     199

  Fragments arranged by size: 

                1206     305     229     199     164
 
BsrDI GCAATG_nn'

Cuts at:      0     271    2103 
   Size:        271    1832
 
BsrFI r'CCGG_y

Cuts at:      0      39      45     430    1504    1819    1873    2103 
   Size:         39       6     385    1074     315      54     230

  Fragments arranged by size: 

                1074     385     315     230      54      39       6
 
BssSI C'ACGA_G

Cuts at:      0     117     910    1048    2060    2103 
   Size:        117     793     138    1012      43

  Fragments arranged by size: 

                1012     793     138     117      43
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     156     806    1896    2103 
   Size:        156     650    1090     207

  Fragments arranged by size: 

                1090     650     207     156
 
BstEII G'GTnAC_C

Cuts at:      0     792    2103 
   Size:        792    1311
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1074    1206    2103 
   Size:       1074     132     897

  Fragments arranged by size: 

                1074     897     132
 
BstYI r'GATC_y

Cuts at:      0     704    2103 
   Size:        704    1399
 
Bsu36I CC'TnA_GG

Cuts at:      0     862     989    2103 
   Size:        862     127    1114

  Fragments arranged by size: 

                1114     862     127
 
BtgI C'CryG_G

Cuts at:      0     193     365     647     709     878     971    1034    1262 
   Size:        193     172     282      62     169      93      63     228

Cuts at:   1262    1381    1430    2103 
   Size:        119      49     673

  Fragments arranged by size: 

                 673     282     228     193     172     169     119      93
                  63      62      49
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     246    2103 
   Size:        246    1857
 
BtsI GCAGTG_nn'

Cuts at:      0     129     628    1170    2103 
   Size:        129     499     542     933

  Fragments arranged by size: 

                 933     542     499     129
 
DraIII CAC_nnn'GTG

Cuts at:      0     806    1833    2103 
   Size:        806    1027     270

  Fragments arranged by size: 

                1027     806     270
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1239    1414    2103 
   Size:       1239     175     689

  Fragments arranged by size: 

                1239     689     175
 
EaeI y'GGCC_r

Cuts at:      0     278     428     902    1196    1433    1502    1506    1817 
   Size:        278     150     474     294     237      69       4     311

Cuts at:   1817    2103 
   Size:        286

  Fragments arranged by size: 

                 474     311     294     286     278     237     150      69
                   4
 
EagI C'GGCC_G

Cuts at:      0    1506    1817    2103 
   Size:       1506     311     286

  Fragments arranged by size: 

                1506     311     286
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     600    1093    1407    2103 
   Size:        600     493     314     696

  Fragments arranged by size: 

                 696     600     493     314
 
EcoICRI GAG'CTC

Cuts at:      0     420     837    2103 
   Size:        420     417    1266

  Fragments arranged by size: 

                1266     420     417
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     360     406    2101    2103 
   Size:        112     248      46    1695       2

  Fragments arranged by size: 

                1695     248     112      46       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1363    1990    2103 
   Size:       1363     627     113

  Fragments arranged by size: 

                1363     627     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153     576     653     721     993    1355    1356    1374 
   Size:        153     423      77      68     272     362       1      18

Cuts at:   1374    1587    1756    1773    1893    1913    1914    1995    1996 
   Size:        213     169      17     120      20       1      81       1

Cuts at:   1996    2103 
   Size:        107

  Fragments arranged by size: 

                 423     362     272     213     169     153     120     107
                  81      77      68      20      18      17       1       1
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1674    2103 
   Size:       1674     429
 
FseI GG_CCGG'CC

Cuts at:      0    1508    2103 
   Size:       1508     595
 
FspI TGC'GCA

Cuts at:      0    1363    2103 
   Size:       1363     740
 
HaeII r_GCGC'y

Cuts at:      0      63     145     767     942    1190    1259    1288    2103 
   Size:         63      82     622     175     248      69      29     815

  Fragments arranged by size: 

                 815     622     248     175      82      69      63      29
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     205     237    2103 
   Size:        205      32    1866

  Fragments arranged by size: 

                1866     205      32
 
KasI G'GCGC_C

Cuts at:      0     763    2103 
   Size:        763    1340
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      75    2103 
   Size:         75    2028
 
MscI TGG'CCA

Cuts at:      0     904    1198    2103 
   Size:        904     294     905

  Fragments arranged by size: 

                 905     904     294
 
MslI CAynn'nnrTG

Cuts at:      0     247     556     898     910    1264    1441    2103 
   Size:        247     309     342      12     354     177     662

  Fragments arranged by size: 

                 662     354     342     309     247     177      12
 
MspA1I CmG'CkG

Cuts at:      0     113     195     426     711     873    1239    1296    1383 
   Size:        113      82     231     285     162     366      57      87

Cuts at:   1383    1432    1635    1816    1854    2025    2103 
   Size:         49     203     181      38     171      78

  Fragments arranged by size: 

                 366     285     231     203     181     171     162     113
                  87      82      78      57      49      38
 
NaeI GCC'GGC

Cuts at:      0     432    1506    1821    2103 
   Size:        432    1074     315     282

  Fragments arranged by size: 

                1074     432     315     282
 
NarI GG'CG_CC

Cuts at:      0     764    2103 
   Size:        764    1339
 
NcoI C'CATG_G

Cuts at:      0     647    2103 
   Size:        647    1456
 
NgoMIV G'CCGG_C

Cuts at:      0     430    1504    1819    2103 
   Size:        430    1074     315     284

  Fragments arranged by size: 

                1074     430     315     284
 
NheI G'CTAG_C

Cuts at:      0     822    1935    2103 
   Size:        822    1113     168

  Fragments arranged by size: 

                1113     822     168
 
NspI r_CATG'y

Cuts at:      0     361    2103 
   Size:        361    1742
 
PasI CC'CwG_GG

Cuts at:      0    1764    1917    2103 
   Size:       1764     153     186

  Fragments arranged by size: 

                1764     186     153
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     104     173    1833    2103 
   Size:        104      69    1660     270

  Fragments arranged by size: 

                1660     270     104      69
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2011    2043    2103 
   Size:       2011      32      60

  Fragments arranged by size: 

                2011      60      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     153     576     993    1374    1773    1893    2103 
   Size:        153     423     417     381     399     120     210

  Fragments arranged by size: 

                 423     417     399     381     210     153     120
 
PshAI GACnn'nnGTC

Cuts at:      0    1741    2103 
   Size:       1741     362
 
PspOMI G'GGCC_C

Cuts at:      0     721    1355    1779    1913    1995    2103 
   Size:        721     634     424     134      82     108

  Fragments arranged by size: 

                 721     634     424     134     108      82
 
PspXI vC'TCGA_Gb

Cuts at:      0     948    2103 
   Size:        948    1155
 
PstI C_TGCA'G

Cuts at:      0      26     290     461    1667    1892    1948    2103 
   Size:         26     264     171    1206     225      56     155

  Fragments arranged by size: 

                1206     264     225     171     155      56      26
 
PvuII CAG'CTG

Cuts at:      0     113     426    1239    2025    2103 
   Size:        113     313     813     786      78

  Fragments arranged by size: 

                 813     786     313     113      78
 
RsrII CG'GwC_CG

Cuts at:      0      48    2103 
   Size:         48    2055
 
SacI G_AGCT'C

Cuts at:      0     422     839    2103 
   Size:        422     417    1264

  Fragments arranged by size: 

                1264     422     417
 
SacII CC_GC'GG

Cuts at:      0     196     712    1384    1433    2103 
   Size:        196     516     672      49     670

  Fragments arranged by size: 

                 672     670     516     196      49
 
SexAI A'CCwGG_T

Cuts at:      0     572    2103 
   Size:        572    1531
 
SfcI C'TryA_G

Cuts at:      0      22     286     297     457    1309    1663    1674    1888 
   Size:         22     264      11     160     852     354      11     214

Cuts at:   1888    1944    2103 
   Size:         56     159

  Fragments arranged by size: 

                 852     354     264     214     160     159      56      22
                  11      11
 
SfoI GGC'GCC

Cuts at:      0     765    2103 
   Size:        765    1338
 
SmaI CCC'GGG

Cuts at:      0      73    2001    2103 
   Size:         73    1928     102

  Fragments arranged by size: 

                1928     102      73
 
SmlI C'TyrA_G

Cuts at:      0     948    2103 
   Size:        948    1155
 
StyI C'CwwG_G

Cuts at:      0     647    2103 
   Size:        647    1456
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1528    2103 
   Size:       1528     575
 
TatI w'GTAC_w

Cuts at:      0    1637    2103 
   Size:       1637     466
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1283    1315    1684    1716    2103 
   Size:       1283      32     369      32     387

  Fragments arranged by size: 

                1283     387     369      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     790    2103 
   Size:        790    1313
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     885     940    1191    1443    1800    2103 
   Size:        885      55     251     252     357     303

  Fragments arranged by size: 

                 885     357     303     252     251      55
 
XhoI C'TCGA_G

Cuts at:      0     948    2103 
   Size:        948    1155
 
XmaI C'CCGG_G

Cuts at:      0      71    1999    2103 
   Size:         71    1928     104

  Fragments arranged by size: 

                1928     104      71
 
ZraI GAC'GTC

Cuts at:      0     216    2103 
   Size:        216    1887
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI
    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FseI     FspI    HaeII    Hin4I
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NspI     PasI    PflMI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PstI    PvuII    RsrII     SacI    SacII    SexAI
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI    BglII     BmrI
    BpuEI     BsaI    BsaAI    BsaWI    BsiWI     BsmI    BspEI    BspHI
    BsrBI    BsrGI   BssHII    BstBI  BstZ17I     ClaI    CspCI     DraI
     EarI    EcoRI    EcoRV    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     NdeI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI     PsiI     PsrI     PvuI     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XmnI
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