Restriction map for pF1KB3818
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BbeI        BbrPI       BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaWI       BseAI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoT14I     EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MaeIII      
MamI        MfeI        MluI        MroI        MseI        
MunI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SbfI        
ScaI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tru9I       Tsp45I      XbaI        XcmI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII         382  GACGTC               GACGT^C
AflIII          9  ACGTGT               A^C[GA][CT]GT
AspHI         604  GTGCTC               G[AT]GC[AT]^C
AspI          853  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          455  TGGCCA               TGG^CCA
BanI           76  GGTGCC               G^G[CT][GA]CC
BbsI          126  GAAGAC               GAAGAC(2/6)
BbvCI         217  GCTGAGG              CCTCAGC(-5/-2)
BcgI          567  GCAAAGCCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          335  TGATCA               T^GATCA
BfaI          260  CTAG                 C^TAG
BpuAI         126  GAAGAC               GAAGAC(2/6)
BsaI         1194  GGTCTC               GGTCTC(1/5)
BsiEI        1110  CGACCG               CG[GA][CT]^CG
BsiHKAI       604  GTGCTC               G[AT]GC[AT]^C
BsmBI        1161  CGTCTC               CGTCTC(1/5)
EcoRI         739  GAATTC               G^AATTC
EcoRV          64  GATATC               GAT^ATC
FbaI          335  TGATCA               T^GATCA
HaeII         662  AGCGCC               [GA]GCGC^[CT]
HgaI          472  GACGC                GACGC(5/10)
HinfI         897  GAATC                G^A[ACGT]TC
MaeI          260  CTAG                 C^TAG
MluNI         455  TGGCCA               TGG^CCA
MscI          455  TGGCCA               TGG^CCA
MslI         1141  CACAGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        142  CAGCTG               C[AC]G^C[GT]G
NaeI         1071  GCCGGC               GCC^GGC
NgoMIV       1071  GCCGGC               G^CCGGC
NspI          872  ACATGC               [GA]CATG^[CT]
PaeR7I        265  CTCGAG               C^TCGAG
PflFI         853  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         200  CCATCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         142  CAGCTG               CAG^CTG
SalI         1108  GTCGAC               G^TCGAC
SapI          813  GCTCTTC              GCTCTTC(1/4)
SexAI         428  ACCTGGT              A^CC[AT]GGT
TfiI          897  GAATC                G^A[AT]TC
Tth111I       853  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        200  CCATCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          265  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          433  GTCTAC               GT^[AC][GT]AC
             1108  GTCGAC              
AcyI          382  GACGTC               G[GA]^CG[CT]C
              472  GACGCC              
BamHI         191  GGATCC               G^GATCC
             1202  GGATCC              
BpmI          115  CTGGAG               CTGGAG(16/14)
              586  CTGGAG              
BsaAI           8  TACGTG               [CT]AC^GT[GA]
              601  TACGTG              
BsaHI         382  GACGTC               G[GA]^CG[CT]C
              472  GACGCC              
BsmAI        1162  GTCTC                GTCTC(1/5)
             1195  GTCTC               
BsrI          866  ACTGG                ACTGG(1/-1)
             1180  ACTGG               
BstXI         107  CCATCAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1068  CCAGCCGGCTGG        
BstYI         191  GGATCC               [GA]^GATC[CT]
             1202  GGATCC              
CfoI          663  GCGC                 GCG^C
             1150  GCGC                
DdeI          218  CTGAG                C^T[ACGT]AG
             1170  CTCAG               
DsaI          998  CCACGG               C^C[GA][CT]GG
             1040  CCGTGG              
EaeI          323  TGGCCG               [CT]^GGCC[GA]
              455  TGGCCA              
EarI          814  CTCTTC               CTCTTC(1/4)
              826  CTCTTC              
HhaI          663  GCGC                 GCG^C
             1150  GCGC                
Hin1I         382  GACGTC               G[GA]^CG[CT]C
              472  GACGCC              
HinP1I        663  GCGC                 G^CGC
             1150  GCGC                
HincII        699  GTCAAC               GT[CT]^[GA]AC
             1108  GTCGAC              
HindII        699  GTCAAC               GT[CT]^[GA]AC
             1108  GTCGAC              
HphI           94  GGTGA                GGTGA(8/7)
             1125  GGTGA               
Ksp632I       814  CTCTTC               CTCTTC(1/4)
              826  CTCTTC              
MflI          191  GGATCC               [GA]^GATC[CT]
             1202  GGATCC              
SmlI          265  CTCGAG               C^T[CT][GA]AG
              304  CTCAAG              
XhoII         191  GGATCC               [GA]^GATC[CT]
             1202  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BssKI       BstF5I      
BstNI       BstUI       Cac8I       Cfr10I      Cfr13I      
DpnII       DraII       EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MaeII       MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TseI        Tsp509I     TspRI       TthHB8I     
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