Restriction map for pF1KB3818
 (Linear) (Six-base) MAPSORT of: KIBB3818.seq  Check: 2110  from: 1  to: 1248



KIBB3818.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AatII G_ACGT'C

Cuts at:      0     386    1248 
   Size:        386     862
 
AccI GT'mk_AC

Cuts at:      0     434    1109    1248 
   Size:        434     675     139

  Fragments arranged by size: 

                 675     434     139
 
AflIII A'CryG_T

Cuts at:      0       9    1248 
   Size:          9    1239
 
AleI CACnn'nnGTG

Cuts at:      0    1145    1248 
   Size:       1145     103
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1163    1248 
   Size:       1163      85
 
ApoI r'AATT_y

Cuts at:      0     295     739     880    1248 
   Size:        295     444     141     368

  Fragments arranged by size: 

                 444     368     295     141
 
AvaI C'yCGr_G

Cuts at:      0     265     901     974    1240    1248 
   Size:        265     636      73     266       8

  Fragments arranged by size: 

                 636     266     265      73       8
 
BamHI G'GATC_C

Cuts at:      0     191    1202    1248 
   Size:        191    1011      46

  Fragments arranged by size: 

                1011     191      46
 
BanI G'GyrC_C

Cuts at:      0      76    1248 
   Size:         76    1172
 
BanII G_rGCy'C

Cuts at:      0     628     647     981    1040    1248 
   Size:        628      19     334      59     208

  Fragments arranged by size: 

                 628     334     208      59      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     133    1248 
   Size:        133    1115
 
BbvCI CC'TCA_GC

Cuts at:      0     218    1248 
   Size:        218    1030
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     556     590    1248 
   Size:        556      34     658

  Fragments arranged by size: 

                 658     556      34
 
BclI T'GATC_A

Cuts at:      0     335    1248 
   Size:        335     913
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1190    1248 
   Size:       1190      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     607    1248 
   Size:        136     471     641

  Fragments arranged by size: 

                 641     471     136
 
Bpu10I CC'TnA_GC

Cuts at:      0     218    1248 
   Size:        218    1030
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289    1248 
   Size:        289     959
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1200    1248 
   Size:       1200      48
 
BsaAI yAC'GTr

Cuts at:      0      10     603    1248 
   Size:         10     593     645

  Fragments arranged by size: 

                 645     593      10
 
BsaHI Gr'CG_yC

Cuts at:      0     383     473    1248 
   Size:        383      90     775

  Fragments arranged by size: 

                 775     383      90
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     610     640    1150    1180    1248 
   Size:        610      30     510      30      68

  Fragments arranged by size: 

                 610     510      68      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     955    1155    1158    1248 
   Size:        955     200       3      90

  Fragments arranged by size: 

                 955     200      90       3
 
BseYI C'CCAG_C

Cuts at:      0     140     252     411    1206    1248 
   Size:        140     112     159     795      42

  Fragments arranged by size: 

                 795     159     140     112      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     908    1123    1248 
   Size:        130     778     215     125

  Fragments arranged by size: 

                 778     215     130     125
 
BsiEI CG_ry'CG

Cuts at:      0    1113    1248 
   Size:       1113     135
 
BsiHKAI G_wGCw'C

Cuts at:      0     608    1248 
   Size:        608     640
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1167    1248 
   Size:       1167      81
 
Bsp1286I G_dGCh'C

Cuts at:      0     608     628     647     981    1040    1248 
   Size:        608      20      19     334      59     208

  Fragments arranged by size: 

                 608     334     208      59      20      19
 
BsrBI CCG'CTC

Cuts at:      0      50     767     921    1248 
   Size:         50     717     154     327

  Fragments arranged by size: 

                 717     327     154      50
 
BsrFI r'CCGG_y

Cuts at:      0     520     833    1016    1071    1248 
   Size:        520     313     183      55     177

  Fragments arranged by size: 

                 520     313     183     177      55
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     114    1075    1248 
   Size:        114     961     173

  Fragments arranged by size: 

                 961     173     114
 
BstYI r'GATC_y

Cuts at:      0     191    1202    1248 
   Size:        191    1011      46

  Fragments arranged by size: 

                1011     191      46
 
BtgI C'CryG_G

Cuts at:      0     998    1040    1248 
   Size:        998      42     208

  Fragments arranged by size: 

                 998     208      42
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      69    1248 
   Size:         69    1179
 
BtsI GCAGTG_nn'

Cuts at:      0    1007    1032    1248 
   Size:       1007      25     216

  Fragments arranged by size: 

                1007     216      25
 
EaeI y'GGCC_r

Cuts at:      0     323     455    1248 
   Size:        323     132     793

  Fragments arranged by size: 

                 793     323     132
 
EarI CTCTTCn'nnn_

Cuts at:      0     820     832    1248 
   Size:        820      12     416

  Fragments arranged by size: 

                 820     416      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     607    1248 
   Size:        136     471     641

  Fragments arranged by size: 

                 641     471     136
 
EcoO109I rG'GnC_Cy

Cuts at:      0      53     256     270     714    1248 
   Size:         53     203      14     444     534

  Fragments arranged by size: 

                 534     444     203      53      14
 
EcoRI G'AATT_C

Cuts at:      0     739    1248 
   Size:        739     509
 
EcoRV GAT'ATC

Cuts at:      0      66    1248 
   Size:         66    1182
 
HaeII r_GCGC'y

Cuts at:      0     666    1248 
   Size:        666     582
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26      58    1248 
   Size:         26      32    1190

  Fragments arranged by size: 

                1190      32      26
 
HincII GTy'rAC

Cuts at:      0     701    1110    1248 
   Size:        701     409     138

  Fragments arranged by size: 

                 701     409     138
 
MscI TGG'CCA

Cuts at:      0     457    1248 
   Size:        457     791
 
MslI CAynn'nnrTG

Cuts at:      0    1145    1248 
   Size:       1145     103
 
MspA1I CmG'CkG

Cuts at:      0     144    1248 
   Size:        144    1104
 
NaeI GCC'GGC

Cuts at:      0    1073    1248 
   Size:       1073     175
 
NgoMIV G'CCGG_C

Cuts at:      0    1071    1248 
   Size:       1071     177
 
NspI r_CATG'y

Cuts at:      0     876    1248 
   Size:        876     372
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     206    1248 
   Size:        206    1042
 
PfoI T'CCnGG_A

Cuts at:      0    1198    1248 
   Size:       1198      50
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     145     177     259     291    1248 
   Size:        145      32      82      32     957

  Fragments arranged by size: 

                 957     145      82      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      53     270     714    1248 
   Size:         53     217     444     534

  Fragments arranged by size: 

                 534     444     217      53
 
PspXI vC'TCGA_Gb

Cuts at:      0     265    1248 
   Size:        265     983
 
PvuII CAG'CTG

Cuts at:      0     144    1248 
   Size:        144    1104
 
SalI G'TCGA_C

Cuts at:      0    1108    1248 
   Size:       1108     140
 
SapI GCTCTTCn'nnn_

Cuts at:      0     820    1248 
   Size:        820     428
 
SexAI A'CCwGG_T

Cuts at:      0     428    1248 
   Size:        428     820
 
SmlI C'TyrA_G

Cuts at:      0     265     304    1248 
   Size:        265      39     944

  Fragments arranged by size: 

                 944     265      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      64     496    1011    1248 
   Size:         64     432     515     237

  Fragments arranged by size: 

                 515     432     237      64
 
TatI w'GTAC_w

Cuts at:      0     611    1248 
   Size:        611     637
 
Tth111I GACn'n_nGTC

Cuts at:      0     856    1248 
   Size:        856     392
 
XhoI C'TCGA_G

Cuts at:      0     265    1248 
   Size:        265     983
 
ZraI GAC'GTC

Cuts at:      0     384    1248 
   Size:        384     864
 

 Enzymes that do cut:

    AatII     AccI   AflIII     AleI     AlfI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BcgI     BclI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BsrFI    BstXI
    BstYI     BtgI    BtgZI     BtsI     EaeI     EarI  Eco57MI EcoO109I
    EcoRI    EcoRV    HaeII    Hin4I   HincII     MscI     MslI   MspA1I
     NaeI   NgoMIV     NspI    PflMI     PfoI     PpiI    PpuMI    PspXI
    PvuII     SalI     SapI    SexAI     SmlI    TaqII     TatI  Tth111I
     XhoI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BsaBI    BsaWI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI    PshAI     PsiI   PspOMI     PsrI     PstI
     PvuI    RsrII     SacI    SacII    SanDI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI     XbaI     XcmI
     XmaI     XmnI
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