Restriction map for pF1KB3493
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     CelII       ClaI        
CpoI        DrdI        Eco47III    EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspI        SwaI        Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          493  GAATTT               [GA]^AATT[CT]
AhdI         1105  GACCCAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          603  CCTGAGG              CC^T[ACGT]AGG
ApaI         1608  GGGCCC               GGGCC^C
ApoI          493  GAATTT               [GA]^AATT[CT]
Asp700I      1560  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1105  GACCCAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1783  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1334  TGGCCA               TGG^CCA
BamHI        1529  GGATCC               G^GATCC
BanI         1861  GGTGCC               G^G[CT][GA]CC
BbsI          348  GAAGAC               GAAGAC(2/6)
BglII        1670  AGATCT               A^GATCT
BpuAI         348  GAAGAC               GAAGAC(2/6)
BsmBI         115  GAGACG               CGTCTC(1/5)
BsmI          303  GAATGC               GAATGC(1/-1)
Bsp1407I      626  TGTACA               T^GTACA
BspMI         389  ACCTGC               ACCTGC(4/8)
BsrGI         626  TGTACA               T^GTACA
Bsu36I        603  CCTGAGG              CC^T[ACGT]AGG
DraI         1242  TTTAAA               TTT^AAA
DraII        1607  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DraIII         75  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1752  CCACGG               C^C[GA][CT]GG
Eam1105I     1105  GACCCAGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        603  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1607  AGGGCCC              [GA]G^G[ACGT]CC[CT]
HincII        808  GTCAAC               GT[CT]^[GA]AC
HindII        808  GTCAAC               GT[CT]^[GA]AC
HindIII      1204  AAGCTT               A^AGCTT
MluNI        1334  TGGCCA               TGG^CCA
MscI         1334  TGGCCA               TGG^CCA
NruI          447  TCGCGA               TCG^CGA
PflFI        1783  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         184  CAGCTG               CAG^CTG
SbfI          228  CCTGCAGG             CCTGCA^GG
SfiI          234  GGCCGACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          245  CCCGGG               CCC^GGG
Sse8387I      228  CCTGCAGG             CCTGCA^GG
SspBI         626  TGTACA               T^GTACA
StuI          551  AGGCCT               AGG^CCT
Tsp509I       494  AATT                 ^AATT
Tth111I      1783  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          581  CCAGCGCCGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         245  CCCGGG               C^CCGGG
XmaI          245  CCCGGG               C^CCGGG
XmnI         1560  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          166  GTCTAC               GT^[AC][GT]AC
              964  GTCTAC              
AfaI          627  GTAC                 GT^AC
             1833  GTAC                
AvaII         423  GGACC                G^G[AT]CC
             1788  GGTCC               
BglI          235  GCCGACCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              307  GCCCAGGTGGC         
BsmFI         567  GTCCC                GGGAC(10/14)
             1186  GTCCC               
BstAPI        222  GCAGTTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              681  GCAGGATGTGC         
BstEII         69  GGTCACC              G^GT[ACGT]ACC
             1087  GGTGACC             
BstPI          69  GGTCACC              G^GT[ACGT]ACC
             1087  GGTGACC             
EagI         1465  CGGCCG               C^GGCCG
             1626  CGGCCG              
EclXI        1465  CGGCCG               C^GGCCG
             1626  CGGCCG              
Eco52I       1465  CGGCCG               C^GGCCG
             1626  CGGCCG              
EcoO65I        69  GGTCACC              G^GT[ACGT]ACC
             1087  GGTGACC             
EcoT14I      1313  CCAAGG               C^C[AT][AT]GG
             1715  CCAAGG              
HaeII         583  AGCGCC               [GA]GCGC^[CT]
             1636  AGCGCC              
HinfI         837  GATTC                G^A[ACGT]TC
              947  GAATC               
MseI         1243  TTAA                 T^TAA
             1370  TTAA                
MspA1I        184  CAGCTG               C[AC]G^C[GT]G
              430  CCGCTG              
RsaI          627  GTAC                 GT^AC
             1833  GTAC                
SacI         1069  GAGCTC               GAGCT^C
             1351  GAGCTC              
SmlI          844  CTCAAG               C^T[CT][GA]AG
             1306  CTCAAG              
StyI         1313  CCAAGG               C^C[AT][AT]GG
             1715  CCAAGG              
TfiI          837  GATTC                G^A[AT]TC
              947  GAATC               
Tru9I        1243  TTAA                 T^TAA
             1370  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       AspHI       AvaI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       EaeI        
EarI        EcoNI       EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XhoII       
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