Restriction map for pF1KB3493
 (Linear) (Six-base) MAPSORT of: KIBB3493.seq  Check: 4590  from: 1  to: 1890



KIBB3493.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     398    1890 
   Size:        398    1492
 
AccI GT'mk_AC

Cuts at:      0     167     965    1890 
   Size:        167     798     925

  Fragments arranged by size: 

                 925     798     167
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    1063    1890 
   Size:        352     711     827

  Fragments arranged by size: 

                 827     711     352
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1110    1890 
   Size:       1110     780
 
AleI CACnn'nnGTG

Cuts at:      0    1726    1890 
   Size:       1726     164
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      27    1890 
   Size:         27    1863
 
AlwNI CAG_nnn'CTG

Cuts at:      0     228     894    1503    1890 
   Size:        228     666     609     387

  Fragments arranged by size: 

                 666     609     387     228
 
ApaI G_GGCC'C

Cuts at:      0    1612    1890 
   Size:       1612     278
 
ApoI r'AATT_y

Cuts at:      0     493    1890 
   Size:        493    1397
 
AvaI C'yCGr_G

Cuts at:      0     245     505     973    1890 
   Size:        245     260     468     917

  Fragments arranged by size: 

                 917     468     260     245
 
BamHI G'GATC_C

Cuts at:      0    1529    1890 
   Size:       1529     361
 
BanI G'GyrC_C

Cuts at:      0    1861    1890 
   Size:       1861      29
 
BanII G_rGCy'C

Cuts at:      0      56     136    1073    1224    1355    1612    1890 
   Size:         56      80     937     151     131     257     278

  Fragments arranged by size: 

                 937     278     257     151     131      80      56
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     355    1890 
   Size:        355    1535
 
BglI GCCn_nnn'nGGC

Cuts at:      0     241     313    1890 
   Size:        241      72    1577

  Fragments arranged by size: 

                1577     241      72
 
BglII A'GATC_T

Cuts at:      0    1670    1890 
   Size:       1670     220
 
Bme1580I G_kGCm'C

Cuts at:      0      16     151    1612    1890 
   Size:         16     135    1461     278

  Fragments arranged by size: 

                1461     278     135      16
 
BmgBI CAC'GTC

Cuts at:      0     119     353    1890 
   Size:        119     234    1537

  Fragments arranged by size: 

                1537     234     119
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1073    1548    1890 
   Size:       1073     475     342

  Fragments arranged by size: 

                1073     475     342
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     789    1890 
   Size:        789    1101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     613     943    1048    1115    1342    1378    1890 
   Size:         70     543     330     105      67     227      36     512

  Fragments arranged by size: 

                 543     512     330     227     105      70      67      36
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     571    1349    1890 
   Size:        571     778     541

  Fragments arranged by size: 

                 778     571     541
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     829    1291    1890 
   Size:        829     462     599

  Fragments arranged by size: 

                 829     599     462
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     760    1152    1318    1890 
   Size:        760     392     166     572

  Fragments arranged by size: 

                 760     572     392     166
 
BsaHI Gr'CG_yC

Cuts at:      0     271     646     758    1890 
   Size:        271     375     112    1132

  Fragments arranged by size: 

                1132     375     271     112
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      19      49    1337    1367    1624    1654    1890 
   Size:         19      30    1288      30     257      30     236

  Fragments arranged by size: 

                1288     257     236      30      30      30      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40     811     832     892    1890 
   Size:         40     771      21      60     998

  Fragments arranged by size: 

                 998     771      60      40      21
 
BseYI C'CCAG_C

Cuts at:      0     295     473    1107    1198    1503    1890 
   Size:        295     178     634      91     305     387

  Fragments arranged by size: 

                 634     387     305     295     178      91
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     275    1495    1890 
   Size:        172     103    1220     395

  Fragments arranged by size: 

                1220     395     172     103
 
BsiEI CG_ry'CG

Cuts at:      0     181    1468    1629    1890 
   Size:        181    1287     161     261

  Fragments arranged by size: 

                1287     261     181     161
 
BsiHKAI G_wGCw'C

Cuts at:      0      40     389     512     657    1021    1073    1355    1890 
   Size:         40     349     123     145     364      52     282     535

  Fragments arranged by size: 

                 535     364     349     282     145     123      52      40
            
 
BsmI GAATG_Cn'

Cuts at:      0     309    1890 
   Size:        309    1581
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     109    1890 
   Size:        109    1781
 
Bsp1286I G_dGCh'C

Cuts at:      0      16      40      56     136     151     389     512     657 
   Size:         16      24      16      80      15     238     123     145

Cuts at:    657    1021    1073    1224    1355    1612    1890 
   Size:        364      52     151     131     257     278

  Fragments arranged by size: 

                 364     278     257     238     151     145     131     123
                  80      52      24      16      16      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     398    1890 
   Size:        398    1492
 
BsrFI r'CCGG_y

Cuts at:      0      66    1463    1601    1890 
   Size:         66    1397     138     289

  Fragments arranged by size: 

                1397     289     138      66
 
BsrGI T'GTAC_A

Cuts at:      0     626    1890 
   Size:        626    1264
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     228     687    1890 
   Size:        228     459    1203

  Fragments arranged by size: 

                1203     459     228
 
BstEII G'GTnAC_C

Cuts at:      0      69    1087    1890 
   Size:         69    1018     803

  Fragments arranged by size: 

                1018     803      69
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48    1320    1728    1890 
   Size:         48    1272     408     162

  Fragments arranged by size: 

                1272     408     162      48
 
BstYI r'GATC_y

Cuts at:      0     860    1529    1670    1890 
   Size:        860     669     141     220

  Fragments arranged by size: 

                 860     669     220     141
 
Bsu36I CC'TnA_GG

Cuts at:      0     604    1890 
   Size:        604    1286
 
BtgI C'CryG_G

Cuts at:      0    1752    1890 
   Size:       1752     138
 
BtsI GCAGTG_nn'

Cuts at:      0     160     324    1054    1512    1890 
   Size:        160     164     730     458     378

  Fragments arranged by size: 

                 730     458     378     164     160
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1551    1586    1890 
   Size:       1551      35     304

  Fragments arranged by size: 

                1551     304      35
 
DraI TTT'AAA

Cuts at:      0    1244    1890 
   Size:       1244     646
 
DraIII CAC_nnn'GTG

Cuts at:      0      80    1890 
   Size:         80    1810
 
EaeI y'GGCC_r

Cuts at:      0      20      84    1334    1465    1626    1890 
   Size:         20      64    1250     131     161     264

  Fragments arranged by size: 

                1250     264     161     131      64      20
 
EagI C'GGCC_G

Cuts at:      0    1465    1626    1890 
   Size:       1465     161     264

  Fragments arranged by size: 

                1465     264     161
 
EarI CTCTTCn'nnn_

Cuts at:      0     788     796     847    1890 
   Size:        788       8      51    1043

  Fragments arranged by size: 

                1043     788      51       8
 
EcoICRI GAG'CTC

Cuts at:      0    1071    1353    1890 
   Size:       1071     282     537

  Fragments arranged by size: 

                1071     537     282
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     352     613     943    1048    1063    1115    1342 
   Size:         70     282     261     330     105      15      52     227

Cuts at:   1342    1378    1890 
   Size:         36     512

  Fragments arranged by size: 

                 512     330     282     261     227     105      70      52
                  36      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      22     334     574     777    1890 
   Size:         22     312     240     203    1113

  Fragments arranged by size: 

                1113     312     240     203      22
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1608    1890 
   Size:       1608     282
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     372     768    1581    1890 
   Size:        372     396     813     309

  Fragments arranged by size: 

                 813     396     372     309
 
HaeII r_GCGC'y

Cuts at:      0     587    1640    1890 
   Size:        587    1053     250

  Fragments arranged by size: 

                1053     587     250
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     916     948    1096    1128    1337    1369 
   Size:         16      32     868      32     148      32     209      32

Cuts at:   1369    1890 
   Size:        521

  Fragments arranged by size: 

                 868     521     209     148      32      32      32      32
                  16
 
HincII GTy'rAC

Cuts at:      0     810    1890 
   Size:        810    1080
 
HindIII A'AGCT_T

Cuts at:      0    1204    1890 
   Size:       1204     686
 
MscI TGG'CCA

Cuts at:      0    1336    1890 
   Size:       1336     554
 
MslI CAynn'nnrTG

Cuts at:      0     144     739    1726    1890 
   Size:        144     595     987     164

  Fragments arranged by size: 

                 987     595     164     144
 
MspA1I CmG'CkG

Cuts at:      0     186     432    1890 
   Size:        186     246    1458

  Fragments arranged by size: 

                1458     246     186
 
NruI TCG'CGA

Cuts at:      0     449    1890 
   Size:        449    1441
 
PfoI T'CCnGG_A

Cuts at:      0     419    1064    1355    1379    1890 
   Size:        419     645     291      24     511

  Fragments arranged by size: 

                 645     511     419     291      24
 
PspOMI G'GGCC_C

Cuts at:      0    1608    1890 
   Size:       1608     282
 
PstI C_TGCA'G

Cuts at:      0     233     395     437    1269    1556    1890 
   Size:        233     162      42     832     287     334

  Fragments arranged by size: 

                 832     334     287     233     162      42
 
PvuII CAG'CTG

Cuts at:      0     186    1890 
   Size:        186    1704
 
SacI G_AGCT'C

Cuts at:      0    1073    1355    1890 
   Size:       1073     282     535

  Fragments arranged by size: 

                1073     535     282
 
SbfI CC_TGCA'GG

Cuts at:      0     233    1890 
   Size:        233    1657
 
SfcI C'TryA_G

Cuts at:      0     229     391     433    1265    1552    1890 
   Size:        229     162      42     832     287     338

  Fragments arranged by size: 

                 832     338     287     229     162      42
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     241    1890 
   Size:        241    1649
 
SmaI CCC'GGG

Cuts at:      0     247    1890 
   Size:        247    1643
 
SmlI C'TyrA_G

Cuts at:      0     844    1306    1890 
   Size:        844     462     584

  Fragments arranged by size: 

                 844     584     462
 
StuI AGG'CCT

Cuts at:      0     553    1890 
   Size:        553    1337
 
StyI C'CwwG_G

Cuts at:      0    1313    1715    1890 
   Size:       1313     402     175

  Fragments arranged by size: 

                1313     402     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     309    1890 
   Size:        309    1581
 
TatI w'GTAC_w

Cuts at:      0     626    1890 
   Size:        626    1264
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      19      51    1890 
   Size:         19      32    1839

  Fragments arranged by size: 

                1839      32      19
 
Tth111I GACn'n_nGTC

Cuts at:      0    1786    1890 
   Size:       1786     104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     588    1890 
   Size:        588    1302
 
XmaI C'CCGG_G

Cuts at:      0     245    1890 
   Size:        245    1645
 
XmnI GAAnn'nnTTC

Cuts at:      0    1564    1890 
   Size:       1564     326
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     AleI     AlfI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrFI    BsrGI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI     DraI   DraIII     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII  HindIII     MscI     MslI   MspA1I     NruI     PfoI
   PspOMI     PstI    PvuII     SacI     SbfI     SfcI     SfiI     SmaI
     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BlpI     BmtI    BsaAI    BsaBI
    BsaWI    BsiWI    BspEI    BspHI    BsrBI    BsrDI   BssHII    BssSI
    BstBI  BstZ17I    BtgZI     ClaI     DrdI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI
     XhoI     ZraI
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