Restriction map for pF1KB3492
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# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsiEI       BsiHKAI     BsiWI       
BsoBI       Bsp1407I    BspDI       BspHI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MslI        MunI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccI          434  GTATAC               GT^[AC][GT]AC
AccII          41  CGCG                 CG^CG
AccIII        144  TCCGGA               T^CCGGA
AfaI          607  GTAC                 GT^AC
AflIII        174  ACATGT               A^C[GA][CT]GT
AlwI           16  GATCC                GGATC(4/5)
BalI          585  TGGCCA               TGG^CCA
BanI           67  GGTGCC               G^G[CT][GA]CC
BanII          99  GGGCTC               G[GA]GC[CT]^C
BfaI          626  CTAG                 C^TAG
BglI          133  GCCACTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI           99  GGGCTC               G[AGT]GC[ACT]^C
BsaWI         144  TCCGGA               [AT]^CCGG[AT]
BseAI         144  TCCGGA               T^CCGGA
BseRI         374  CTCCTC               GAGGAG(10/8)
BsgI          227  GTGCAG               GTGCAG(16/14)
BsmBI          30  CGTCTC               CGTCTC(1/5)
Bsp1286I       99  GGGCTC               G[AGT]GC[ACT]^C
BspEI         144  TCCGGA               T^CCGGA
BspLU11I      174  ACATGT               A^CATGT
BspMI         922  GCAGGT               ACCTGC(4/8)
BsrDI        1171  GCAATG               GCAATG(2/0)
Bst1107I      434  GTATAC               GTA^TAC
BstF5I       1160  GGATG                GGATG(2/0)
BstUI          41  CGCG                 CG^CG
BstYI          15  AGATCC               [GA]^GATC[CT]
BstZ17I       434  GTATAC               GTA^TAC
CfoI           40  GCGC                 GCG^C
DraII          92  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          669  CCATGG               C^C[GA][CT]GG
EaeI          585  TGGCCA               [CT]^GGCC[GA]
EcoO109I       92  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       458  ATGCAT               ATGCA^T
FokI         1160  GGATG                GGATG(9/13)
HapII         145  CCGG                 C^CGG
HhaI           40  GCGC                 GCG^C
HinP1I         40  GCGC                 G^CGC
HpaII         145  CCGG                 C^CGG
MaeI          626  CTAG                 C^TAG
MaeII         813  ACGT                 A^CGT
MflI           15  AGATCC               [GA]^GATC[CT]
MluNI         585  TGGCCA               TGG^CCA
MroI          144  TCCGGA               T^CCGGA
MscI          585  TGGCCA               TGG^CCA
MspI          145  CCGG                 C^CGG
MvnI           41  CGCG                 CG^CG
NcoI          669  CCATGG               C^CATGG
NdeI          475  CATATG               CA^TATG
NsiI          458  ATGCAT               ATGCA^T
NspI          174  ACATGT               [GA]CATG^[CT]
PleI          328  GACTC                GAGTC(4/5)
PpuMI          92  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          773  CTGCAG               CTGCA^G
RsaI          607  GTAC                 GT^AC
SmlI          390  CTTGAG               C^T[CT][GA]AG
SspI         1027  AATATT               AAT^ATT
StuI          282  AGGCCT               AGG^CCT
TaqI         1080  TCGA                 T^CGA
TfiI          201  GAATC                G^A[AT]TC
TthHB8I      1080  TCGA                 T^CGA
XcmI          264  CCAGATGATCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          15  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI           37  GCGG                 CCGC(-3/-1)
               42  GCGG                
AcsI          233  GAATTC               [GA]^AATT[CT]
             1082  GAATTC              
ApoI          233  GAATTC               [GA]^AATT[CT]
             1082  GAATTC              
BpmI          330  CTCCAG               CTGGAG(16/14)
             1108  CTGGAG              
BsmFI         552  GTCCC                GGGAC(10/14)
              849  GTCCC               
BsmI            3  GAATGC               GAATGC(1/-1)
              570  GAATGC              
BstXI         332  CCAGCATTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1056  CCACCCTCTTGG        
EarI          126  GAAGAG               CTCTTC(1/4)
              629  GAAGAG              
EcoRI         233  GAATTC               G^AATTC
             1082  GAATTC              
EcoT14I       669  CCATGG               C^C[AT][AT]GG
             1049  CCTTGG              
HincII        621  GTCAAC               GT[CT]^[GA]AC
              943  GTTGAC              
HindII        621  GTCAAC               GT[CT]^[GA]AC
              943  GTTGAC              
HinfI         201  GAATC                G^A[ACGT]TC
              328  GACTC               
HphI          412  GGTGA                GGTGA(8/7)
              737  TCACC               
Ksp632I       126  GAAGAG               CTCTTC(1/4)
              629  GAAGAG              
PflMI          90  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              503  CCAAAAAATGG         
SfcI          773  CTGCAG               C^T[GA][CT]AG
             1043  CTATAG              
StyI          669  CCATGG               C^C[AT][AT]GG
             1049  CCTTGG              
Van91I         90  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              503  CCAAAAAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AluI        AlwNI       AvaII       BbvI        BsaJI       
BsiYI       BslI        BsmAI       BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      HaeIII      ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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