Restriction map for pF1KB3475
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BsaAI       BsaHI       BsaI        
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstYI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     KasI        KpnI        KspI        
MfeI        MflI        MluI        MroI        MunI        
NarI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AfaI          393  GTAC                 GT^AC
AlwI          366  GATCC                GGATC(4/5)
AlwNI         844  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          461  GGGCCC               GGGCC^C
BanI          469  GGCACC               G^G[CT][GA]CC
BfaI         1063  CTAG                 C^TAG
BglI         1019  GCCTCGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          567  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      567  GCTGAGC              GC^T[ACGT]AGC
BsaBI         360  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         937  TCCGGT               [AT]^CCGG[AT]
BsmAI        1360  GAGAC                GTCTC(1/5)
BspHI        1049  TCATGA               T^CATGA
BsrBI         789  CCGCTC               CCGCTC(-3/-3)
BsrDI        1209  CATTGC               GCAATG(2/0)
BssSI         882  CACGAG               CACGAG(-5/-1)
BstAPI        747  GCAGATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        768  GGTCACC              G^GT[ACGT]ACC
BstPI         768  GGTCACC              G^GT[ACGT]ACC
CelII         567  GCTGAGC              GC^T[ACGT]AGC
EcoO65I       768  GGTCACC              G^GT[ACGT]ACC
EcoRI         279  GAATTC               G^AATTC
EcoT14I      1159  CCATGG               C^C[AT][AT]GG
HpaI          259  GTTAAC               GTT^AAC
MaeI         1063  CTAG                 C^TAG
MamI          360  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          260  TTAA                 T^TAA
NcoI         1159  CCATGG               C^CATGG
NheI         1062  GCTAGC               G^CTAGC
NspI         1256  GCATGT               [GA]CATG^[CT]
PshAI         138  GACATGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1049  TCATGA               T^CATGA
RsaI          393  GTAC                 GT^AC
SbfI          720  CCTGCAGG             CCTGCA^GG
Sse8387I      720  CCTGCAGG             CCTGCA^GG
StuI         1017  AGGCCT               AGG^CCT
StyI         1159  CCATGG               C^C[AT][AT]GG
TfiI         1465  GATTC                G^A[AT]TC
Tru9I         260  TTAA                 T^TAA
XcmI          843  CCAGCAGCTGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII         694  CGCG                 CG^CG
             1059  CGCG                
AcsI          279  GAATTC               [GA]^AATT[CT]
             1242  AAATTT              
ApoI          279  GAATTC               [GA]^AATT[CT]
             1242  AAATTT              
AspHI         322  GTGCTC               G[AT]GC[AT]^C
             1315  GTGCTC              
AvaI          622  CCCGAG               C^[CT]CG[GA]G
             1435  CCCGAG              
BalI          206  TGGCCA               TGG^CCA
             1109  TGGCCA              
BanII           4  GAGCCC               G[GA]GC[CT]^C
              461  GGGCCC              
BbsI          868  GTCTTC               GAAGAC(2/6)
             1297  GTCTTC              
BcgI          488  GCATCGTCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              500  GCATCCTCATCG        
BcnI          585  CCCGG                CC^[GC]GG
              666  CCGGG               
BpuAI         868  GTCTTC               GAAGAC(2/6)
             1297  GTCTTC              
BsgI          475  CTGCAC               GTGCAG(16/14)
              907  GTGCAG              
BsiHKAI       322  GTGCTC               G[AT]GC[AT]^C
             1315  GTGCTC              
BsoBI         622  CCCGAG               C^[CT]CG[GA]G
             1435  CCCGAG              
BspMI         299  ACCTGC               ACCTGC(4/8)
              719  ACCTGC              
BsrFI         272  GCCGGC               [GA]^CCGG[CT]
              995  GCCGGC              
BstUI         694  CGCG                 CG^CG
             1059  CGCG                
BstXI         147  CCACCGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              422  CCATGTATGTGG        
CfoI          693  GCGC                 GCG^C
             1060  GCGC                
Cfr10I        272  GCCGGC               [GA]^CCGG[CT]
              995  GCCGGC              
DsaI           44  CCGTGG               C^C[GA][CT]GG
             1159  CCATGG              
EarI         1180  CTCTTC               CTCTTC(1/4)
              736  GAAGAG              
HhaI          693  GCGC                 GCG^C
             1060  GCGC                
HinP1I        693  GCGC                 G^CGC
             1060  GCGC                
HincII        259  GTTAAC               GT[CT]^[GA]AC
              946  GTCAAC              
HindII        259  GTTAAC               GT[CT]^[GA]AC
              946  GTCAAC              
Ksp632I      1180  CTCTTC               CTCTTC(1/4)
              736  GAAGAG              
MluNI         206  TGGCCA               TGG^CCA
             1109  TGGCCA              
MscI          206  TGGCCA               TGG^CCA
             1109  TGGCCA              
MvnI          694  CGCG                 CG^CG
             1059  CGCG                
NaeI          272  GCCGGC               GCC^GGC
              995  GCCGGC              
NciI          585  CCCGG                CC^[GC]GG
              666  CCGGG               
NgoMIV        272  GCCGGC               G^CCGGC
              995  GCCGGC              
PpuMI         548  AGGACCT              [GA]G^G[AT]CC[CT]
             1143  GGGTCCT             
Tsp509I       280  AATT                 ^AATT
             1243  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PstI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      
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