Restriction map for pF1KB3475
 (Linear) (Six-base) MAPSORT of: KIBB3475.seq  Check: 3591  from: 1  to: 1476



KIBB3475.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1167    1476 
   Size:       1167     309
 
AleI CACnn'nnGTG

Cuts at:      0     964    1476 
   Size:        964     512
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     625    1476 
   Size:        625     851
 
AlwNI CAG_nnn'CTG

Cuts at:      0     849    1476 
   Size:        849     627
 
ApaI G_GGCC'C

Cuts at:      0     465    1476 
   Size:        465    1011
 
ApoI r'AATT_y

Cuts at:      0     279    1242    1476 
   Size:        279     963     234

  Fragments arranged by size: 

                 963     279     234
 
AvaI C'yCGr_G

Cuts at:      0     622    1435    1476 
   Size:        622     813      41

  Fragments arranged by size: 

                 813     622      41
 
BanI G'GyrC_C

Cuts at:      0     469    1476 
   Size:        469    1007
 
BanII G_rGCy'C

Cuts at:      0       8     465    1476 
   Size:          8     457    1011

  Fragments arranged by size: 

                1011     457       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     861    1290    1476 
   Size:        861     429     186

  Fragments arranged by size: 

                 861     429     186
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     477     489     511     523    1476 
   Size:        477      12      22      12     953

  Fragments arranged by size: 

                 953     477      22      12      12
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1025    1476 
   Size:       1025     451
 
BlpI GC'TnA_GC

Cuts at:      0     568    1476 
   Size:        568     908
 
Bme1580I G_kGCm'C

Cuts at:      0     422     465     472    1476 
   Size:        422      43       7    1004

  Fragments arranged by size: 

                1004     422      43       7
 
BmgBI CAC'GTC

Cuts at:      0     708    1198    1476 
   Size:        708     490     278

  Fragments arranged by size: 

                 708     490     278
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     352    1476 
   Size:        352    1124
 
BmtI G_CTAG'C

Cuts at:      0    1066    1476 
   Size:       1066     410
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     799    1093    1476 
   Size:        110     689     294     383

  Fragments arranged by size: 

                 689     383     294     110
 
BsaBI GATnn'nnATC

Cuts at:      0     364    1476 
   Size:        364    1112
 
BsaWI w'CCGG_w

Cuts at:      0     937    1476 
   Size:        937     539
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      41      71    1267    1297    1476 
   Size:         41      30    1196      30     179

  Fragments arranged by size: 

                1196     179      41      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      65     136     673    1453    1476 
   Size:         65      71     537     780      23

  Fragments arranged by size: 

                 780     537      71      65      23
 
BseYI C'CCAG_C

Cuts at:      0       7     483     842    1476 
   Size:          7     476     359     634

  Fragments arranged by size: 

                 634     476     359       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460     928    1476 
   Size:        460     468     548

  Fragments arranged by size: 

                 548     468     460
 
BsiHKAI G_wGCw'C

Cuts at:      0     326    1319    1476 
   Size:        326     993     157

  Fragments arranged by size: 

                 993     326     157
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     326     422     465     472    1319    1476 
   Size:          8     318      96      43       7     847     157

  Fragments arranged by size: 

                 847     318     157      96      43       8       7
 
BspHI T'CATG_A

Cuts at:      0    1049    1476 
   Size:       1049     427
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     308     728    1476 
   Size:        308     420     748

  Fragments arranged by size: 

                 748     420     308
 
BsrBI CCG'CTC

Cuts at:      0     791    1476 
   Size:        791     685
 
BsrDI GCAATG_nn'

Cuts at:      0    1208    1476 
   Size:       1208     268
 
BsrFI r'CCGG_y

Cuts at:      0     272     995    1476 
   Size:        272     723     481

  Fragments arranged by size: 

                 723     481     272
 
BssSI C'ACGA_G

Cuts at:      0     882    1476 
   Size:        882     594
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     753    1476 
   Size:        753     723
 
BstEII G'GTnAC_C

Cuts at:      0     768    1476 
   Size:        768     708
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     154     429    1476 
   Size:        154     275    1047

  Fragments arranged by size: 

                1047     275     154
 
BtgI C'CryG_G

Cuts at:      0      44    1159    1476 
   Size:         44    1115     317

  Fragments arranged by size: 

                1115     317      44
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     492     804    1041    1476 
   Size:        492     312     237     435

  Fragments arranged by size: 

                 492     435     312     237
 
BtsI GCAGTG_nn'

Cuts at:      0      62     604    1066    1476 
   Size:         62     542     462     410

  Fragments arranged by size: 

                 542     462     410      62
 
EaeI y'GGCC_r

Cuts at:      0      41     206     270    1109    1476 
   Size:         41     165      64     839     367

  Fragments arranged by size: 

                 839     367     165      64      41
 
EarI CTCTTCn'nnn_

Cuts at:      0     731    1186    1476 
   Size:        731     455     290

  Fragments arranged by size: 

                 731     455     290
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     291     306    1014    1476 
   Size:        291      15     708     462

  Fragments arranged by size: 

                 708     462     291      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     799    1093    1167    1476 
   Size:        110     689     294      74     309

  Fragments arranged by size: 

                 689     309     294     110      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0     461     462     549    1144    1476 
   Size:        461       1      87     595     332

  Fragments arranged by size: 

                 595     461     332      87       1
 
EcoRI G'AATT_C

Cuts at:      0     279    1476 
   Size:        279    1197
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     993    1025    1080    1112    1476 
   Size:        993      32      55      32     364

  Fragments arranged by size: 

                 993     364      55      32      32
 
HincII GTy'rAC

Cuts at:      0     261     948    1476 
   Size:        261     687     528

  Fragments arranged by size: 

                 687     528     261
 
HpaI GTT'AAC

Cuts at:      0     261    1476 
   Size:        261    1215
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1253    1476 
   Size:       1253     223
 
MscI TGG'CCA

Cuts at:      0     208    1111    1476 
   Size:        208     903     365

  Fragments arranged by size: 

                 903     365     208
 
MslI CAynn'nnrTG

Cuts at:      0     152     388     427     823     964    1048    1476 
   Size:        152     236      39     396     141      84     428

  Fragments arranged by size: 

                 428     396     236     152     141      84      39
 
MspA1I CmG'CkG

Cuts at:      0     483     703     837     849    1204    1476 
   Size:        483     220     134      12     355     272

  Fragments arranged by size: 

                 483     355     272     220     134      12
 
NaeI GCC'GGC

Cuts at:      0     274     997    1476 
   Size:        274     723     479

  Fragments arranged by size: 

                 723     479     274
 
NcoI C'CATG_G

Cuts at:      0    1159    1476 
   Size:       1159     317
 
NgoMIV G'CCGG_C

Cuts at:      0     272     995    1476 
   Size:        272     723     481

  Fragments arranged by size: 

                 723     481     272
 
NheI G'CTAG_C

Cuts at:      0    1062    1476 
   Size:       1062     414
 
NspI r_CATG'y

Cuts at:      0    1260    1476 
   Size:       1260     216
 
PasI CC'CwG_GG

Cuts at:      0     465    1455    1476 
   Size:        465     990      21

  Fragments arranged by size: 

                 990     465      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     191    1160    1272    1455    1476 
   Size:        191     969     112     183      21

  Fragments arranged by size: 

                 969     191     183     112      21
 
PfoI T'CCnGG_A

Cuts at:      0     776    1476 
   Size:        776     700
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1429    1461    1476 
   Size:       1429      32      15

  Fragments arranged by size: 

                1429      32      15
 
PpuMI rG'GwC_Cy

Cuts at:      0     549    1144    1476 
   Size:        549     595     332

  Fragments arranged by size: 

                 595     549     332
 
PshAI GACnn'nnGTC

Cuts at:      0     142    1476 
   Size:        142    1334
 
PspOMI G'GGCC_C

Cuts at:      0     461    1476 
   Size:        461    1015
 
PstI C_TGCA'G

Cuts at:      0      74     599     725     836    1476 
   Size:         74     525     126     111     640

  Fragments arranged by size: 

                 640     525     126     111      74
 
PvuII CAG'CTG

Cuts at:      0     483     703     837     849    1204    1476 
   Size:        483     220     134      12     355     272

  Fragments arranged by size: 

                 483     355     272     220     134      12
 
SbfI CC_TGCA'GG

Cuts at:      0     725    1476 
   Size:        725     751
 
SfcI C'TryA_G

Cuts at:      0      70     595     721     832    1476 
   Size:         70     525     126     111     644

  Fragments arranged by size: 

                 644     525     126     111      70
 
StuI AGG'CCT

Cuts at:      0    1019    1476 
   Size:       1019     457
 
StyI C'CwwG_G

Cuts at:      0    1159    1476 
   Size:       1159     317
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     421     459    1476 
   Size:        421      38    1017

  Fragments arranged by size: 

                1017     421      38
 
TatI w'GTAC_w

Cuts at:      0     392    1476 
   Size:        392    1084
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      41      73    1267    1299    1476 
   Size:         41      32    1194      32     177

  Fragments arranged by size: 

                1194     177      41      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     850    1476 
   Size:        850     626
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbsI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstXI     BtgI    BtgZI     BtsI     EaeI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    Hin4I   HincII     HpaI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI   PspOMI     PstI
    PvuII     SbfI     SfcI     StuI     StyI    TaqII     TatI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI    BfrBI    BglII
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrGI   BssHII    BstBI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     KasI     KpnI     MfeI     MluI     NarI     NdeI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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