Restriction map for pF1KB3404
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AcyI        AgeI        Alw44I      
Aor51HI     ApaLI       AscI        Asp718I     AvaI        
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BcnI        BlnI        
BlpI        Bpu1102I    BsaHI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BsrDI       
BsrFI       BssHII      BstEII      BstPI       CelII       
Cfr10I      ClaI        CpoI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoT22I     
FseI        Hin1I       KasI        KpnI        MluI        
NaeI        NarI        NciI        NgoMIV      NotI        
NruI        NsiI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RsrII       SalI        SbfI        ScaI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         2381  GTATAC               GT^[AC][GT]AC
AccII        5056  CGCG                 CG^CG
AccIII       4891  TCCGGA               T^CCGGA
AclI         1614  AACGTT               AA^CGTT
AflIII       3633  ACATGT               A^C[GA][CT]GT
AocI         4678  CCTCAGG              CC^T[ACGT]AGG
ApaI           49  GGGCCC               GGGCC^C
AspI          983  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BclI         3705  TGATCA               T^GATCA
BglI         2674  GCCAAACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1943  TACGTG               [CT]AC^GT[GA]
BsaBI        3969  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        4891  TCCGGA               [AT]^CCGG[AT]
BseAI        4891  TCCGGA               T^CCGGA
BspEI        4891  TCCGGA               T^CCGGA
BspLU11I     3633  ACATGT               A^CATGT
BspMI        3885  GCAGGT               ACCTGC(4/8)
Bst1107I     2381  GTATAC               GTA^TAC
BstBI         953  TTCGAA               TT^CGAA
BstUI        5056  CGCG                 CG^CG
BstXI        1265  CCACCTCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2381  GTATAC               GTA^TAC
Bsu36I       4678  CCTCAGG              CC^T[ACGT]AGG
Eco81I       4678  CCTCAGG              CC^T[ACGT]AGG
EcoRV        4288  GATATC               GAT^ATC
FbaI         3705  TGATCA               T^GATCA
HaeII        1346  AGCGCC               [GA]GCGC^[CT]
HapII        4892  CCGG                 C^CGG
HpaI         2313  GTTAAC               GTT^AAC
HpaII        4892  CCGG                 C^CGG
KspI         5055  CCGCGG               CCGC^GG
MamI         3969  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          563  CAATTG               C^AATTG
MroI         4891  TCCGGA               T^CCGGA
MspI         4892  CCGG                 C^CGG
MunI          563  CAATTG               C^AATTG
MvnI         5056  CGCG                 CG^CG
NdeI         3128  CATATG               CA^TATG
NspV          953  TTCGAA               TT^CGAA
PflFI         983  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     1614  AACGTT               AA^CGTT
PstI         1414  CTGCAG               CTGCA^G
SacI         3851  GAGCTC               GAGCT^C
SacII        5055  CCGCGG               CCGC^GG
SapI         2806  GAAGAGC              GCTCTTC(1/4)
SfuI          953  TTCGAA               TT^CGAA
StuI         2516  AGGCCT               AGG^CCT
Tth111I       983  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1880  CCAAAAAAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AhdI          790  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3081  GACAAAAGGTC         
AseI         4398  ATTAAT               AT^TAAT
             4584  ATTAAT              
AsnI         4398  ATTAAT               AT^TAAT
             4584  ATTAAT              
AspEI         790  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3081  GACAAAAGGTC         
AviII        1862  TGCGCA               TGC^GCA
             4117  TGCGCA              
BbsI          223  GAAGAC               GAAGAC(2/6)
             1003  GAAGAC              
BpuAI         223  GAAGAC               GAAGAC(2/6)
             1003  GAAGAC              
BseRI         254  GAGGAG               GAGGAG(10/8)
             4545  GAGGAG              
BspHI         881  TCATGA               T^CATGA
             3588  TCATGA              
BssSI        2501  CACGAG               CACGAG(-5/-1)
             2576  CACGAG              
BstAPI       1854  GCAAGAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4854  GCAGCCCATGC         
Eam1105I      790  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3081  GACAAAAGGTC         
EcoNI        3106  CCTCAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4741  CCTGAGCCAGG         
EcoRI        1732  GAATTC               G^AATTC
             4822  GAATTC              
FspI         1862  TGCGCA               TGC^GCA
             4117  TGCGCA              
HgaI         1699  GACGC                GACGC(5/10)
             2604  GCGTC               
HincII       1392  GTCAAC               GT[CT]^[GA]AC
             2313  GTTAAC              
HindII       1392  GTCAAC               GT[CT]^[GA]AC
             2313  GTTAAC              
MaeII        1615  ACGT                 A^CGT
             1944  ACGT                
NheI         3420  GCTAGC               G^CTAGC
             4765  GCTAGC              
PshBI        4398  ATTAAT               AT^TAAT
             4584  ATTAAT              
PvuII        2788  CAGCTG               CAG^CTG
             3158  CAGCTG              
RcaI          881  TCATGA               T^CATGA
             3588  TCATGA              
SexAI         452  ACCTGGT              A^CC[AT]GGT
             1823  ACCTGGT             
SpeI         1213  ACTAGT               A^CTAGT
             1650  ACTAGT              
XbaI         1302  TCTAGA               T^CTAGA
             4198  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BalI        BanII       BbvI        BfaI        
BfrI        BglII       BmyI        BpmI        BsaI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BsrBI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DrdI        DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HindIII     HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmnI        
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