Restriction map for pF1KB3404
 (Linear) (Six-base) MAPSORT of: KIBB3404.seq  Check: 1141  from: 1  to: 5073



KIBB3404.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AccI GT'mk_AC

Cuts at:      0    2382    5073 
   Size:       2382    2691
 
AclI AA'CG_TT

Cuts at:      0    1615    5073 
   Size:       1615    3458
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     542     691    1343    1940    2017    2339    2497 
   Size:         60     482     149     652     597      77     322     158

Cuts at:   2497    2558    2825    3254    3963    4497    5073 
   Size:         61     267     429     709     534     576

  Fragments arranged by size: 

                 709     652     597     576     534     482     429     322
                 267     158     149      77      61      60
 
AflII C'TTAA_G

Cuts at:      0    1848    2152    2376    5073 
   Size:       1848     304     224    2697

  Fragments arranged by size: 

                2697    1848     304     224
 
AflIII A'CryG_T

Cuts at:      0    3633    5073 
   Size:       3633    1440
 
AhdI GACnn_n'nnGTC

Cuts at:      0     795    3086    5073 
   Size:        795    2291    1987

  Fragments arranged by size: 

                2291    1987     795
 
AlwNI CAG_nnn'CTG

Cuts at:      0     709    3430    4650    5073 
   Size:        709    2721    1220     423

  Fragments arranged by size: 

                2721    1220     709     423
 
ApaI G_GGCC'C

Cuts at:      0      53    5073 
   Size:         53    5020
 
ApoI r'AATT_y

Cuts at:      0     502    1107    1137    1732    2266    2559    3295    3496 
   Size:        502     605      30     595     534     293     736     201

Cuts at:   3496    3931    3941    4822    5073 
   Size:        435      10     881     251

  Fragments arranged by size: 

                 881     736     605     595     534     502     435     293
                 251     201      30      10
 
AseI AT'TA_AT

Cuts at:      0    4399    4585    5073 
   Size:       4399     186     488

  Fragments arranged by size: 

                4399     488     186
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2491    2524    5073 
   Size:       2491      33    2549

  Fragments arranged by size: 

                2549    2491      33
 
BanII G_rGCy'C

Cuts at:      0      53    3855    4882    5073 
   Size:         53    3802    1027     191

  Fragments arranged by size: 

                3802    1027     191      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     230    1010    5073 
   Size:        230     780    4063

  Fragments arranged by size: 

                4063     780     230
 
BclI T'GATC_A

Cuts at:      0    3705    5073 
   Size:       3705    1368
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2680    5073 
   Size:       2680    2393
 
BglII A'GATC_T

Cuts at:      0    1635    2415    2815    4127    4195    4656    5073 
   Size:       1635     780     400    1312      68     461     417

  Fragments arranged by size: 

                1635    1312     780     461     417     400      68
 
Bme1580I G_kGCm'C

Cuts at:      0      53    5073 
   Size:         53    5020
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3248    5002    5073 
   Size:       3248    1754      71

  Fragments arranged by size: 

                3248    1754      71
 
BmtI G_CTAG'C

Cuts at:      0    3424    4769    5073 
   Size:       3424    1345     304

  Fragments arranged by size: 

                3424    1345     304
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     704    2659    3182    4116    4652    4850    5062 
   Size:         26     678    1955     523     934     536     198     212

Cuts at:   5062    5073 
   Size:         11

  Fragments arranged by size: 

                1955     934     678     536     523     212     198      26
                  11
 
Bpu10I CC'TnA_GC

Cuts at:      0     941    2520    3152    4742    4990    5073 
   Size:        941    1579     632    1590     248      83

  Fragments arranged by size: 

                1590    1579     941     632     248      83
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1372    5073 
   Size:       1372    3701
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3497    3775    4381    5073 
   Size:       3497     278     606     692

  Fragments arranged by size: 

                3497     692     606     278
 
BsaAI yAC'GTr

Cuts at:      0    1945    5073 
   Size:       1945    3128
 
BsaBI GATnn'nnATC

Cuts at:      0    3973    5073 
   Size:       3973    1100
 
BsaWI w'CCGG_w

Cuts at:      0    4891    5073 
   Size:       4891     182
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2632    2662    5073 
   Size:       2632      30    2411

  Fragments arranged by size: 

                2632    2411      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     269    4560    5073 
   Size:        269    4291     513

  Fragments arranged by size: 

                4291     513     269
 
BseYI C'CCAG_C

Cuts at:      0    3380    4920    5063    5073 
   Size:       3380    1540     143      10

  Fragments arranged by size: 

                3380    1540     143      10
 
BsiHKAI G_wGCw'C

Cuts at:      0    1961    2837    3189    3855    5050    5073 
   Size:       1961     876     352     666    1195      23

  Fragments arranged by size: 

                1961    1195     876     666     352      23
 
BsmI GAATG_Cn'

Cuts at:      0     127    2850    4962    5073 
   Size:        127    2723    2112     111

  Fragments arranged by size: 

                2723    2112     127     111
 
Bsp1286I G_dGCh'C

Cuts at:      0      53    1961    2837    3189    3855    4882    5050    5073 
   Size:         53    1908     876     352     666    1027     168      23

  Fragments arranged by size: 

                1908    1027     876     666     352     168      53      23
            
 
BspEI T'CCGG_A

Cuts at:      0    4891    5073 
   Size:       4891     182
 
BspHI T'CATG_A

Cuts at:      0     881    3588    5073 
   Size:        881    2707    1485

  Fragments arranged by size: 

                2707    1485     881
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3876    5073 
   Size:       3876    1197
 
BsrBI CCG'CTC

Cuts at:      0      55    1752    3509    4754    5073 
   Size:         55    1697    1757    1245     319

  Fragments arranged by size: 

                1757    1697    1245     319      55
 
BsrGI T'GTAC_A

Cuts at:      0     591    3630    4268    5073 
   Size:        591    3039     638     805

  Fragments arranged by size: 

                3039     805     638     591
 
BssSI C'ACGA_G

Cuts at:      0    2501    2576    5073 
   Size:       2501      75    2497

  Fragments arranged by size: 

                2501    2497      75
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1860    4860    5073 
   Size:       1860    3000     213

  Fragments arranged by size: 

                3000    1860     213
 
BstBI TT'CG_AA

Cuts at:      0     954    5073 
   Size:        954    4119
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1272    5073 
   Size:       1272    3801
 
BstYI r'GATC_y

Cuts at:      0     945    1275    1635    2415    2815    3102    4127    4195 
   Size:        945     330     360     780     400     287    1025      68

Cuts at:   4195    4563    4646    4656    5073 
   Size:        368      83      10     417

  Fragments arranged by size: 

                1025     945     780     417     400     368     360     330
                 287      83      68      10
 
BstZ17I GTA'TAC

Cuts at:      0    2383    5073 
   Size:       2383    2690
 
Bsu36I CC'TnA_GG

Cuts at:      0    4679    5073 
   Size:       4679     394
 
BtgI C'CryG_G

Cuts at:      0     752    3846    4307    5055    5073 
   Size:        752    3094     461     748      18

  Fragments arranged by size: 

                3094     752     748     461      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2501    5073 
   Size:       2501    2572
 
BtsI GCAGTG_nn'

Cuts at:      0      76    4776    5073 
   Size:         76    4700     297

  Fragments arranged by size: 

                4700     297      76
 
DraI TTT'AAA

Cuts at:      0    1133    2216    3936    5073 
   Size:       1133    1083    1720    1137

  Fragments arranged by size: 

                1720    1137    1133    1083
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     927    3511    3737    5073 
   Size:        927    2584     226    1336

  Fragments arranged by size: 

                2584    1336     927     226
 
EaeI y'GGCC_r

Cuts at:      0    1179    1721    4219    5073 
   Size:       1179     542    2498     854

  Fragments arranged by size: 

                2498    1179     854     542
 
EarI CTCTTCn'nnn_

Cuts at:      0     138    1808    2235    2399    2687    2801    4331    4520 
   Size:        138    1670     427     164     288     114    1530     189

Cuts at:   4520    5073 
   Size:        553

  Fragments arranged by size: 

                1670    1530     553     427     288     189     164     138
                 114
 
EcoICRI GAG'CTC

Cuts at:      0    3853    5073 
   Size:       3853    1220
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26      60     542     691     704    1343    1940    2017 
   Size:         26      34     482     149      13     639     597      77

Cuts at:   2017    2339    2497    2558    2659    2825    3182    3254    3963 
   Size:        322     158      61     101     166     357      72     709

Cuts at:   3963    4116    4497    4652    4850    5062    5073 
   Size:        153     381     155     198     212      11

  Fragments arranged by size: 

                 709     639     597     482     381     357     322     212
                 198     166     158     155     153     149     101      77
                  72      61      34      26      13      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3110    4745    5073 
   Size:       3110    1635     328

  Fragments arranged by size: 

                3110    1635     328
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3088    3888    4627    5067    5073 
   Size:       3088     800     739     440       6

  Fragments arranged by size: 

                3088     800     739     440       6
 
EcoRI G'AATT_C

Cuts at:      0    1732    4822    5073 
   Size:       1732    3090     251

  Fragments arranged by size: 

                3090    1732     251
 
EcoRV GAT'ATC

Cuts at:      0    4290    5073 
   Size:       4290     783
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1381    2778    3617    5073 
   Size:       1381    1397     839    1456

  Fragments arranged by size: 

                1456    1397    1381     839
 
FspI TGC'GCA

Cuts at:      0    1864    4119    5073 
   Size:       1864    2255     954

  Fragments arranged by size: 

                2255    1864     954
 
HaeII r_GCGC'y

Cuts at:      0    1350    5073 
   Size:       1350    3723
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     715     747     781     813    2632    2664    2800    2832 
   Size:        715      32      34      32    1819      32     136      32

Cuts at:   2832    3072    3104    4288    4308    4314    4320    4340    4346 
   Size:        240      32    1184      20       6       6      20       6

Cuts at:   4346    4936    4968    5073 
   Size:        590      32     105

  Fragments arranged by size: 

                1819    1184     715     590     240     136     105      34
                  32      32      32      32      32      32      20      20
                   6       6       6
 
HincII GTy'rAC

Cuts at:      0    1394    2315    5073 
   Size:       1394     921    2758

  Fragments arranged by size: 

                2758    1394     921
 
HindIII A'AGCT_T

Cuts at:      0    1325    1845    2353    5073 
   Size:       1325     520     508    2720

  Fragments arranged by size: 

                2720    1325     520     508
 
HpaI GTT'AAC

Cuts at:      0    2315    5073 
   Size:       2315    2758
 
MfeI C'AATT_G

Cuts at:      0     563    5073 
   Size:        563    4510
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       8    3076    3322    4364    4447    4621    4669    5000 
   Size:          8    3068     246    1042      83     174      48     331

Cuts at:   5000    5009    5073 
   Size:          9      64

  Fragments arranged by size: 

                3068    1042     331     246     174      83      64      48
                   9       8
 
MscI TGG'CCA

Cuts at:      0    1181    1723    4221    5073 
   Size:       1181     542    2498     852

  Fragments arranged by size: 

                2498    1181     852     542
 
MslI CAynn'nnrTG

Cuts at:      0    2135    3864    4067    5073 
   Size:       2135    1729     203    1006

  Fragments arranged by size: 

                2135    1729    1006     203
 
MspA1I CmG'CkG

Cuts at:      0    2790    3160    5057    5073 
   Size:       2790     370    1897      16

  Fragments arranged by size: 

                2790    1897     370      16
 
NcoI C'CATG_G

Cuts at:      0     752    3846    4307    5073 
   Size:        752    3094     461     766

  Fragments arranged by size: 

                3094     766     752     461
 
NdeI CA'TA_TG

Cuts at:      0    3129    5073 
   Size:       3129    1944
 
NheI G'CTAG_C

Cuts at:      0    3420    4765    5073 
   Size:       3420    1345     308

  Fragments arranged by size: 

                3420    1345     308
 
NspI r_CATG'y

Cuts at:      0     362    2134    3637    3863    5073 
   Size:        362    1772    1503     226    1210

  Fragments arranged by size: 

                1772    1503    1210     362     226
 
PasI CC'CwG_GG

Cuts at:      0    3093    5073 
   Size:       3093    1980
 
PciI A'CATG_T

Cuts at:      0    3633    5073 
   Size:       3633    1440
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1198    3969    4313    5073 
   Size:       1198    2771     344     760

  Fragments arranged by size: 

                2771    1198     760     344
 
PfoI T'CCnGG_A

Cuts at:      0     431    4629    5073 
   Size:        431    4198     444

  Fragments arranged by size: 

                4198     444     431
 
PpuMI rG'GwC_Cy

Cuts at:      0    3088    3888    4627    5067    5073 
   Size:       3088     800     739     440       6

  Fragments arranged by size: 

                3088     800     739     440       6
 
PspOMI G'GGCC_C

Cuts at:      0      49    5073 
   Size:         49    5024
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    4427    4459    5073 
   Size:       4427      32     614

  Fragments arranged by size: 

                4427     614      32
 
PstI C_TGCA'G

Cuts at:      0    1418    5073 
   Size:       1418    3655
 
PvuII CAG'CTG

Cuts at:      0    2790    3160    5073 
   Size:       2790     370    1913

  Fragments arranged by size: 

                2790    1913     370
 
SacI G_AGCT'C

Cuts at:      0    3855    5073 
   Size:       3855    1218
 
SacII CC_GC'GG

Cuts at:      0    5058    5073 
   Size:       5058      15
 
SanDI GG'GwC_CC

Cuts at:      0    5067    5073 
   Size:       5067       6
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2801    5073 
   Size:       2801    2272
 
SexAI A'CCwGG_T

Cuts at:      0     452    1823    5073 
   Size:        452    1371    3250

  Fragments arranged by size: 

                3250    1371     452
 
SfcI C'TryA_G

Cuts at:      0     275     710    1414    3194    4839    5073 
   Size:        275     435     704    1780    1645     234

  Fragments arranged by size: 

                1780    1645     704     435     275     234
 
SmlI C'TyrA_G

Cuts at:      0    1351    1848    2152    2376    5073 
   Size:       1351     497     304     224    2697

  Fragments arranged by size: 

                2697    1351     497     304     224
 
SpeI A'CTAG_T

Cuts at:      0    1213    1650    5073 
   Size:       1213     437    3423

  Fragments arranged by size: 

                3423    1213     437
 
SspI AAT'ATT

Cuts at:      0     236     320    1050    5073 
   Size:        236      84     730    4023

  Fragments arranged by size: 

                4023     730     236      84
 
StuI AGG'CCT

Cuts at:      0    2518    5073 
   Size:       2518    2555
 
StyI C'CwwG_G

Cuts at:      0     752    3846    4307    4905    5073 
   Size:        752    3094     461     598     168

  Fragments arranged by size: 

                3094     752     598     461     168
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1034    1786    3441    3799    5073 
   Size:       1034     752    1655     358    1274

  Fragments arranged by size: 

                1655    1274    1034     752     358
 
TatI w'GTAC_w

Cuts at:      0     591    1224    3630    4268    5073 
   Size:        591     633    2406     638     805

  Fragments arranged by size: 

                2406     805     638     633     591
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1388    1420    1929    1961    4201    4233    5073 
   Size:       1388      32     509      32    2240      32     840

  Fragments arranged by size: 

                2240    1388     840     509      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     986    5073 
   Size:        986    4087
 
XbaI T'CTAG_A

Cuts at:      0    1302    4198    5073 
   Size:       1302    2896     875

  Fragments arranged by size: 

                2896    1302     875
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1887    5073 
   Size:       1887    3186
 
XmnI GAAnn'nnTTC

Cuts at:      0     743    2471    3695    3905    4316    5073 
   Size:        743    1728    1224     210     411     757

  Fragments arranged by size: 

                1728    1224     757     743     411     210
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AflII   AflIII     AhdI    AlwNI     ApaI
     ApoI     AseI     BaeI    BanII     BbsI     BclI     BglI    BglII
 Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NspI     PasI     PciI    PflMI     PfoI    PpuMI   PspOMI     PsrI
     PstI    PvuII     SacI    SacII    SanDI     SapI    SexAI     SfcI
     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI     AgeI     AleI     AlfI     AloI
    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BlpI    BmgBI     BplI    BsaHI
     BsgI    BsiEI    BsiWI    BsmBI    BsrDI    BsrFI   BssHII   BstEII
     ClaI    CspCI   DraIII     EagI     EciI     FseI    FspAI     KasI
     KpnI     MluI     NaeI     NarI   NgoMIV     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PvuI
    RsrII     SalI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SwaI     XhoI     XmaI     ZraI
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