Restriction map for pF1KB3338
 (Linear) (Six-base) MAPSORT of: KIBB3338.seq  Check: 2401  from: 1  to: 3483



KIBB3338.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2410    3483 
   Size:       2410    1073
 
AatII G_ACGT'C

Cuts at:      0    3086    3483 
   Size:       3086     397
 
AccI GT'mk_AC

Cuts at:      0    1655    2762    3290    3483 
   Size:       1655    1107     528     193

  Fragments arranged by size: 

                1655    1107     528     193
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607    1047    2155    3078    3145    3440    3483 
   Size:        607     440    1108     923      67     295      43

  Fragments arranged by size: 

                1108     923     607     440     295      67      43
 
AflIII A'CryG_T

Cuts at:      0     260     932    1172    3483 
   Size:        260     672     240    2311

  Fragments arranged by size: 

                2311     672     260     240
 
AgeI A'CCGG_T

Cuts at:      0    1907    3483 
   Size:       1907    1576
 
AleI CACnn'nnGTG

Cuts at:      0    1309    3214    3483 
   Size:       1309    1905     269

  Fragments arranged by size: 

                1905    1309     269
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     919     951    3483 
   Size:        919      32    2532

  Fragments arranged by size: 

                2532     919      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     338     639     897    1254    1758    2235    2367    2576 
   Size:        338     301     258     357     504     477     132     209

Cuts at:   2576    3483 
   Size:        907

  Fragments arranged by size: 

                 907     504     477     357     338     301     258     209
                 132
 
ApaI G_GGCC'C

Cuts at:      0     735    1269    1437    1626    1815    3483 
   Size:        735     534     168     189     189    1668

  Fragments arranged by size: 

                1668     735     534     189     189     168
 
AvaI C'yCGr_G

Cuts at:      0     624    1457    1626    1814    3483 
   Size:        624     833     169     188    1669

  Fragments arranged by size: 

                1669     833     624     188     169
 
AvrII C'CTAG_G

Cuts at:      0    1489    3483 
   Size:       1489    1994
 
BamHI G'GATC_C

Cuts at:      0     645    3483 
   Size:        645    2838
 
BanI G'GyrC_C

Cuts at:      0    1129    2617    3483 
   Size:       1129    1488     866

  Fragments arranged by size: 

                1488    1129     866
 
BanII G_rGCy'C

Cuts at:      0     385     735    1159    1269    1437    1626    1815    2176 
   Size:        385     350     424     110     168     189     189     361

Cuts at:   2176    2216    2260    2383    2393    2496    2611    2744    3258 
   Size:         40      44     123      10     103     115     133     514

Cuts at:   3258    3333    3483 
   Size:         75     150

  Fragments arranged by size: 

                 514     424     385     361     350     189     189     168
                 150     133     123     115     110     103      75      44
                  40      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     447    3287    3428    3483 
   Size:        447    2840     141      55

  Fragments arranged by size: 

                2840     447     141      55
 
BbvCI CC'TCA_GC

Cuts at:      0    2344    2869    3483 
   Size:       2344     525     614

  Fragments arranged by size: 

                2344     614     525
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2453    2487    3483 
   Size:       2453      34     996

  Fragments arranged by size: 

                2453     996      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1151    3483 
   Size:       1151    2332
 
BclI T'GATC_A

Cuts at:      0    2771    3483 
   Size:       2771     712
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1621    1810    3483 
   Size:       1621     189    1673

  Fragments arranged by size: 

                1673    1621     189
 
BglII A'GATC_T

Cuts at:      0     992    2880    3483 
   Size:        992    1888     603

  Fragments arranged by size: 

                1888     992     603
 
BlpI GC'TnA_GC

Cuts at:      0     912    2843    3483 
   Size:        912    1931     640

  Fragments arranged by size: 

                1931     912     640
 
Bme1580I G_kGCm'C

Cuts at:      0     547     735    1269    1437    1626    1815    1935    2520 
   Size:        547     188     534     168     189     189     120     585

Cuts at:   2520    2546    3483 
   Size:         26     937

  Fragments arranged by size: 

                 937     585     547     534     189     189     188     168
                 120      26
 
BmgBI CAC'GTC

Cuts at:      0    1611    3483 
   Size:       1611    1872
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1008    1922    2122    3483 
   Size:       1008     914     200    1361

  Fragments arranged by size: 

                1361    1008     914     200
 
BmtI G_CTAG'C

Cuts at:      0    1231    3483 
   Size:       1231    2252
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3011    3483 
   Size:       3011     472
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178    1145    2407    2758    3427    3483 
   Size:        178     967    1262     351     669      56

  Fragments arranged by size: 

                1262     967     669     351     178      56
 
Bpu10I CC'TnA_GC

Cuts at:      0    1834    2344    2869    3483 
   Size:       1834     510     525     614

  Fragments arranged by size: 

                1834     614     525     510
 
BsaAI yAC'GTr

Cuts at:      0     933    3483 
   Size:        933    2550
 
BsaBI GATnn'nnATC

Cuts at:      0     448    3483 
   Size:        448    3035
 
BsaHI Gr'CG_yC

Cuts at:      0     416    1949    3083    3483 
   Size:        416    1533    1134     400

  Fragments arranged by size: 

                1533    1134     416     400
 
BsaWI w'CCGG_w

Cuts at:      0     614    1907    3483 
   Size:        614    1293    1576

  Fragments arranged by size: 

                1576    1293     614
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3073    3103    3483 
   Size:       3073      30     380

  Fragments arranged by size: 

                3073     380      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82    3121    3483 
   Size:         82    3039     362

  Fragments arranged by size: 

                3039     362      82
 
BseYI C'CCAG_C

Cuts at:      0     378     389    1721    1913    1942    2999    3146    3224 
   Size:        378      11    1332     192      29    1057     147      78

Cuts at:   3224    3483 
   Size:        259

  Fragments arranged by size: 

                1332    1057     378     259     192     147      78      29
                  11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     220     313    1522    2170    3483 
   Size:        139      81      93    1209     648    1313

  Fragments arranged by size: 

                1313    1209     648     139      93      81
 
BsiHKAI G_wGCw'C

Cuts at:      0     153    1829    2383    2393    2744    3258    3483 
   Size:        153    1676     554      10     351     514     225

  Fragments arranged by size: 

                1676     554     514     351     225     153      10
 
BsmI GAATG_Cn'

Cuts at:      0    3003    3483 
   Size:       3003     480
 
Bsp1286I G_dGCh'C

Cuts at:      0     153     385     547     735    1159    1269    1437    1626 
   Size:        153     232     162     188     424     110     168     189

Cuts at:   1626    1815    1829    1935    2176    2216    2260    2383    2393 
   Size:        189      14     106     241      40      44     123      10

Cuts at:   2393    2496    2520    2546    2611    2744    3258    3333    3483 
   Size:        103      24      26      65     133     514      75     150

  Fragments arranged by size: 

                 514     424     241     232     189     189     188     168
                 162     153     150     133     123     110     106     103
                  75      65      44      40      26      24      14      10
            
 
BspEI T'CCGG_A

Cuts at:      0     614    3483 
   Size:        614    2869
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2243    2410    3483 
   Size:       2243     167    1073

  Fragments arranged by size: 

                2243    1073     167
 
BsrDI GCAATG_nn'

Cuts at:      0    1327    3053    3160    3483 
   Size:       1327    1726     107     323

  Fragments arranged by size: 

                1726    1327     323     107
 
BsrFI r'CCGG_y

Cuts at:      0     211    1845    1907    3483 
   Size:        211    1634      62    1576

  Fragments arranged by size: 

                1634    1576     211      62
 
BsrGI T'GTAC_A

Cuts at:      0    3365    3483 
   Size:       3365     118
 
BssSI C'ACGA_G

Cuts at:      0     139    1042    2054    3483 
   Size:        139     903    1012    1429

  Fragments arranged by size: 

                1429    1012     903     139
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2576    3483 
   Size:       2576     907
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1935    3312    3483 
   Size:       1935    1377     171

  Fragments arranged by size: 

                1935    1377     171
 
BstYI r'GATC_y

Cuts at:      0     130     590     645     752     992    2880    3483 
   Size:        130     460      55     107     240    1888     603

  Fragments arranged by size: 

                1888     603     460     240     130     107      55
 
Bsu36I CC'TnA_GG

Cuts at:      0     248    3483 
   Size:        248    3235
 
BtgI C'CryG_G

Cuts at:      0     683    1388    1527    1805    2252    2372    2441    3483 
   Size:        683     705     139     278     447     120      69    1042

  Fragments arranged by size: 

                1042     705     683     447     278     139     120      69
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1418    3483 
   Size:       1418    2065
 
BtsI GCAGTG_nn'

Cuts at:      0     881    1510    1516    2273    2586    2700    3425    3483 
   Size:        881     629       6     757     313     114     725      58

  Fragments arranged by size: 

                 881     757     725     629     313     114      58       6
            
 
ClaI AT'CG_AT

Cuts at:      0    2850    3483 
   Size:       2850     633
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1954    2335    3483 
   Size:       1954     381    1148

  Fragments arranged by size: 

                1954    1148     381
 
EaeI y'GGCC_r

Cuts at:      0     170     680    1613    1802    1865    1892    2249    2885 
   Size:        170     510     933     189      63      27     357     636

Cuts at:   2885    3483 
   Size:        598

  Fragments arranged by size: 

                 933     636     598     510     357     189     170      63
                  27
 
EarI CTCTTCn'nnn_

Cuts at:      0    1096    1913    2275    3483 
   Size:       1096     817     362    1208

  Fragments arranged by size: 

                1208    1096     817     362
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     232    3483 
   Size:        232    3251
 
EcoICRI GAG'CTC

Cuts at:      0    2381    2391    2742    3256    3483 
   Size:       2381      10     351     514     227

  Fragments arranged by size: 

                2381     514     351     227      10
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     607    1047    1145    2155    2407    2758    3078 
   Size:        178     429     440      98    1010     252     351     320

Cuts at:   3078    3145    3427    3440    3483 
   Size:         67     282      13      43

  Fragments arranged by size: 

                1010     440     429     351     320     282     252     178
                  98      67      43      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1237    1488    1780    3483 
   Size:       1237     251     292    1703

  Fragments arranged by size: 

                1703    1237     292     251
 
EcoO109I rG'GnC_Cy

Cuts at:      0     731     877    1265    1266    1373    1433    1434    1622 
   Size:        731     146     388       1     107      60       1     188

Cuts at:   1622    1623    1773    1811    2328    2559    2955    3483 
   Size:          1     150      38     517     231     396     528

  Fragments arranged by size: 

                 731     528     517     396     388     231     188     150
                 146     107      60      38       1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     612    2745    3393    3483 
   Size:        612    2133     648      90

  Fragments arranged by size: 

                2133     648     612      90
 
FspI TGC'GCA

Cuts at:      0     201    3483 
   Size:        201    3282
 
HaeII r_GCGC'y

Cuts at:      0     919    1759    3349    3483 
   Size:        919     840    1590     134

  Fragments arranged by size: 

                1590     919     840     134
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     122     154    1752    1784    2201    2233    2944    2976 
   Size:        122      32    1598      32     417      32     711      32

Cuts at:   2976    3073    3105    3483 
   Size:         97      32     378

  Fragments arranged by size: 

                1598     711     417     378     122      97      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2586    3483 
   Size:       2586     897
 
HindIII A'AGCT_T

Cuts at:      0    3370    3483 
   Size:       3370     113
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     681    3483 
   Size:        681    2802
 
MscI TGG'CCA

Cuts at:      0    1615    1867    1894    2887    3483 
   Size:       1615     252      27     993     596

  Fragments arranged by size: 

                1615     993     596     252      27
 
MslI CAynn'nnrTG

Cuts at:      0     214     725    1171    1309    1445    2513    3214    3310 
   Size:        214     511     446     138     136    1068     701      96

Cuts at:   3310    3483 
   Size:        173

  Fragments arranged by size: 

                1068     701     511     446     214     173     138     136
                  96
 
MspA1I CmG'CkG

Cuts at:      0     205     234     981    1707    2576    2736    3077    3455 
   Size:        205      29     747     726     869     160     341     378

Cuts at:   3455    3483 
   Size:         28

  Fragments arranged by size: 

                 869     747     726     378     341     205     160      29
                  28
 
NheI G'CTAG_C

Cuts at:      0    1227    3483 
   Size:       1227    2256
 
NspI r_CATG'y

Cuts at:      0     219     264    1176    2534    3483 
   Size:        219      45     912    1358     949

  Fragments arranged by size: 

                1358     949     912     219      45
 
PasI CC'CwG_GG

Cuts at:      0    1547    2106    2658    3483 
   Size:       1547     559     552     825

  Fragments arranged by size: 

                1547     825     559     552
 
PciI A'CATG_T

Cuts at:      0     260    1172    3483 
   Size:        260     912    2311

  Fragments arranged by size: 

                2311     912     260
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1322    3483 
   Size:       1322    2161
 
PfoI T'CCnGG_A

Cuts at:      0      83    3101    3231    3483 
   Size:         83    3018     130     252

  Fragments arranged by size: 

                3018     252     130      83
 
PmlI CAC'GTG

Cuts at:      0     933    3483 
   Size:        933    2550
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     340     372    3483 
   Size:        340      32    3111

  Fragments arranged by size: 

                3111     340      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     877    1773    2328    3483 
   Size:        877     896     555    1155

  Fragments arranged by size: 

                1155     896     877     555
 
PshAI GACnn'nnGTC

Cuts at:      0    2039    2484    3483 
   Size:       2039     445     999

  Fragments arranged by size: 

                2039     999     445
 
PspOMI G'GGCC_C

Cuts at:      0     731    1265    1433    1622    1811    3483 
   Size:        731     534     168     189     189    1672

  Fragments arranged by size: 

                1672     731     534     189     189     168
 
PstI C_TGCA'G

Cuts at:      0     986    1742    2363    2581    3071    3483 
   Size:        986     756     621     218     490     412

  Fragments arranged by size: 

                 986     756     621     490     412     218
 
PvuII CAG'CTG

Cuts at:      0     205     981    2736    3455    3483 
   Size:        205     776    1755     719      28

  Fragments arranged by size: 

                1755     776     719     205      28
 
SacI G_AGCT'C

Cuts at:      0    2383    2393    2744    3258    3483 
   Size:       2383      10     351     514     225

  Fragments arranged by size: 

                2383     514     351     225      10
 
SanDI GG'GwC_CC

Cuts at:      0     877    3483 
   Size:        877    2606
 
ScaI AGT'ACT

Cuts at:      0     574    2800    3483 
   Size:        574    2226     683

  Fragments arranged by size: 

                2226     683     574
 
SexAI A'CCwGG_T

Cuts at:      0    1253    3483 
   Size:       1253    2230
 
SfcI C'TryA_G

Cuts at:      0     982    1738    2359    2577    3067    3483 
   Size:        982     756     621     218     490     416

  Fragments arranged by size: 

                 982     756     621     490     416     218
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1621    1810    3483 
   Size:       1621     189    1673

  Fragments arranged by size: 

                1673    1621     189
 
SmaI CCC'GGG

Cuts at:      0    1628    1816    3483 
   Size:       1628     188    1667

  Fragments arranged by size: 

                1667    1628     188
 
StuI AGG'CCT

Cuts at:      0     668    2357    3483 
   Size:        668    1689    1126

  Fragments arranged by size: 

                1689    1126     668
 
StyI C'CwwG_G

Cuts at:      0     266     960    1489    1895    3483 
   Size:        266     694     529     406    1588

  Fragments arranged by size: 

                1588     694     529     406     266
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     622    1321    1795    2323    3483 
   Size:        622     699     474     528    1160

  Fragments arranged by size: 

                1160     699     622     528     474
 
TatI w'GTAC_w

Cuts at:      0     572     782    2798    3218    3365    3483 
   Size:        572     210    2016     420     147     118

  Fragments arranged by size: 

                2016     572     420     210     147     118
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1033    1065    2109    2141    2406    2438    3483 
   Size:       1033      32    1044      32     265      32    1045

  Fragments arranged by size: 

                1045    1044    1033     265      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     451    3286    3483 
   Size:        451    2835     197

  Fragments arranged by size: 

                2835     451     197
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2733    3483 
   Size:       2733     750
 
XmaI C'CCGG_G

Cuts at:      0    1626    1814    3483 
   Size:       1626     188    1669

  Fragments arranged by size: 

                1669    1626     188
 
ZraI GAC'GTC

Cuts at:      0    3084    3483 
   Size:       3084     399
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AgeI     AleI     AloI
    AlwNI     ApaI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     MmeI     MscI     MslI
   MspA1I     NheI     NspI     PasI     PciI    PflMI     PfoI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SanDI
     ScaI    SexAI     SfcI     SfiI     SmaI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AhdI     AlfI    ApaLI     ApoI
     AscI     AseI    AsiSI     BaeI     BbeI    BfrBI    BpuEI     BsaI
    BsiEI    BsiWI    BsmBI    BspHI    BsrBI   BssHII    BstBI   BstEII
  BstZ17I    CspCI     DraI   DraIII     EagI    EcoRI    EcoRV     FseI
    FspAI     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PmeI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI
     SbfI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     XbaI     XhoI     XmnI
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