Restriction map for pF1KB3334
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# NO CUT ENZYMES (  114 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvrII       BbeI        BciVI       BclI        
BfaI        BfrI        BlnI        BlpI        Bpu1102I    
BsaI        BseAI       BsgI        BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KasI        KspI        MaeI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        1723  GACGTC               GACGT^C
Acc65I         57  GGTACC               G^GTACC
AccI          226  GTCTAC               GT^[AC][GT]AC
AccII        1495  CGCG                 CG^CG
AflIII       1466  ACGTGT               A^C[GA][CT]GT
Asp718I        57  GGTACC               G^GTACC
AviII        1544  TGCGCA               TGC^GCA
BamHI        1554  GGATCC               G^GATCC
BanI           57  GGTACC               G^G[CT][GA]CC
BanII        1813  GAGCTC               G[GA]GC[CT]^C
BbrPI        1465  CACGTG               CAC^GTG
BbsI          664  GAAGAC               GAAGAC(2/6)
BcgI          862  CGAGAGGTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI            7  CCCGG                CC^[GC]GG
BglI           34  GCCTGGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1994  AGATCT               A^GATCT
BpuAI         664  GAAGAC               GAAGAC(2/6)
BsaAI        1465  CACGTG               [CT]AC^GT[GA]
BsiEI        1938  CGACCG               CG[GA][CT]^CG
BsmBI         886  GAGACG               CGTCTC(1/5)
BsmI         1863  GCATTC               GAATGC(1/-1)
BspHI        1226  TCATGA               T^CATGA
BspMI         181  ACCTGC               ACCTGC(4/8)
BstAPI        305  GCAAGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1495  CGCG                 CG^CG
DraIII        328  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI        2020  GAATTC               G^AATTC
FspI         1544  TGCGCA               TGC^GCA
HgaI         2215  GACGC                GACGC(5/10)
KpnI           57  GGTACC               GGTAC^C
MseI         1085  TTAA                 T^TAA
MvnI         1495  CGCG                 CG^CG
NciI            7  CCCGG                CC^[GC]GG
PaeR7I       1135  CTCGAG               C^TCGAG
PflMI        1960  CCAGGTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1465  CACGTG               CAC^GTG
PmlI         1465  CACGTG               CAC^GTG
PvuII        1354  CAGCTG               CAG^CTG
RcaI         1226  TCATGA               T^CATGA
SacI         1813  GAGCTC               GAGCT^C
ScaI         1004  AGTACT               AGT^ACT
SexAI        1959  ACCAGGT              A^CC[AT]GGT
Tru9I        1085  TTAA                 T^TAA
Van91I       1960  CCAGGTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          836  CCACAGAGTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1135  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI          400  AAATTC               [GA]^AATT[CT]
             2020  GAATTC              
AcyI         1723  GACGTC               G[GA]^CG[CT]C
             2215  GACGCC              
ApoI          400  AAATTC               [GA]^AATT[CT]
             2020  GAATTC              
AspHI         870  GTGCTC               G[AT]GC[AT]^C
             1813  GAGCTC              
AvaII         173  GGACC                G^G[AT]CC
             1580  GGTCC               
BalI         1373  TGGCCA               TGG^CCA
             1730  TGGCCA              
BbvCI         703  GCTGAGG              CCTCAGC(-5/-2)
              772  GCTGAGG             
BsaBI         157  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2145  GATCGTCATC          
BsaHI        1723  GACGTC               G[GA]^CG[CT]C
             2215  GACGCC              
BsaWI         980  ACCGGA               [AT]^CCGG[AT]
             1577  TCCGGT              
BsiHKAI       870  GTGCTC               G[AT]GC[AT]^C
             1813  GAGCTC              
BsrBI        1433  CCGCTC               CCGCTC(-3/-3)
             1568  CCGCTC              
BsrDI         813  CATTGC               GCAATG(2/0)
              904  GCAATG              
DrdI          613  GACTCGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2215  GACGCCGCTGTC        
DsaI         1808  CCGTGG               C^C[GA][CT]GG
             1858  CCGTGG              
Hin1I        1723  GACGTC               G[GA]^CG[CT]C
             2215  GACGCC              
MamI          157  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2145  GATCGTCATC          
MluNI        1373  TGGCCA               TGG^CCA
             1730  TGGCCA              
MscI         1373  TGGCCA               TGG^CCA
             1730  TGGCCA              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaI        BbvI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EarI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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