Restriction map for pF1KB3334
 (Linear) (Six-base) MAPSORT of: KIBB3334.seq  Check: 4606  from: 1  to: 2310



KIBB3334.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AatII G_ACGT'C

Cuts at:      0    1727    2310 
   Size:       1727     583
 
AccI GT'mk_AC

Cuts at:      0     227    2310 
   Size:        227    2083
 
Acc65I G'GTAC_C

Cuts at:      0      57    2310 
   Size:         57    2253
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     956    1681    2021    2188    2310 
   Size:        590     366     725     340     167     122

  Fragments arranged by size: 

                 725     590     366     340     167     122
 
AflIII A'CryG_T

Cuts at:      0    1466    2310 
   Size:       1466     844
 
AleI CACnn'nnGTG

Cuts at:      0     250    1601    2158    2310 
   Size:        250    1351     557     152

  Fragments arranged by size: 

                1351     557     250     152
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     529    2310 
   Size:        529    1781
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2030    2062    2310 
   Size:       2030      32     248

  Fragments arranged by size: 

                2030     248      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     844    1356    1966    2310 
   Size:        844     512     610     344

  Fragments arranged by size: 

                 844     610     512     344
 
ApoI r'AATT_y

Cuts at:      0     400    2020    2310 
   Size:        400    1620     290

  Fragments arranged by size: 

                1620     400     290
 
AvaI C'yCGr_G

Cuts at:      0      44    1073    1135    2310 
   Size:         44    1029      62    1175

  Fragments arranged by size: 

                1175    1029      62      44
 
BamHI G'GATC_C

Cuts at:      0    1554    2310 
   Size:       1554     756
 
BanI G'GyrC_C

Cuts at:      0      57    2310 
   Size:         57    2253
 
BanII G_rGCy'C

Cuts at:      0    1817    2310 
   Size:       1817     493
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     671    2310 
   Size:        671    1639
 
BbvCI CC'TCA_GC

Cuts at:      0     704     773    2310 
   Size:        704      69    1537

  Fragments arranged by size: 

                1537     704      69
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     851     885    2310 
   Size:        851      34    1425

  Fragments arranged by size: 

                1425     851      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      40    2310 
   Size:         40    2270
 
BglII A'GATC_T

Cuts at:      0    1994    2310 
   Size:       1994     316
 
Bme1580I G_kGCm'C

Cuts at:      0     951    2310 
   Size:        951    1359
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     630     888    2013    2310 
   Size:        630     258    1125     297

  Fragments arranged by size: 

                1125     630     297     258
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73    1438    1837    2310 
   Size:         73    1365     399     473

  Fragments arranged by size: 

                1365     473     399      73
 
Bpu10I CC'TnA_GC

Cuts at:      0     704     773    2310 
   Size:        704      69    1537

  Fragments arranged by size: 

                1537     704      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1033    1213    2310 
   Size:       1033     180    1097

  Fragments arranged by size: 

                1097    1033     180
 
BsaAI yAC'GTr

Cuts at:      0    1467    2310 
   Size:       1467     843
 
BsaBI GATnn'nnATC

Cuts at:      0     161    2149    2310 
   Size:        161    1988     161

  Fragments arranged by size: 

                1988     161     161
 
BsaHI Gr'CG_yC

Cuts at:      0    1724    2216    2310 
   Size:       1724     492      94

  Fragments arranged by size: 

                1724     492      94
 
BsaWI w'CCGG_w

Cuts at:      0     980    1577    2310 
   Size:        980     597     733

  Fragments arranged by size: 

                 980     733     597
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     619     703    1442    1681    2005    2248    2310 
   Size:        619      84     739     239     324     243      62

  Fragments arranged by size: 

                 739     619     324     243     239      84      62
 
BsiEI CG_ry'CG

Cuts at:      0    1941    2310 
   Size:       1941     369
 
BsiHKAI G_wGCw'C

Cuts at:      0     874    1817    2310 
   Size:        874     943     493

  Fragments arranged by size: 

                 943     874     493
 
BsmI GAATG_Cn'

Cuts at:      0    1863    2310 
   Size:       1863     447
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     880    2310 
   Size:        880    1430
 
Bsp1286I G_dGCh'C

Cuts at:      0     874     951    1817    2310 
   Size:        874      77     866     493

  Fragments arranged by size: 

                 874     866     493      77
 
BspHI T'CATG_A

Cuts at:      0    1226    2310 
   Size:       1226    1084
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     190    2310 
   Size:        190    2120
 
BsrBI CCG'CTC

Cuts at:      0    1435    1570    2310 
   Size:       1435     135     740

  Fragments arranged by size: 

                1435     740     135
 
BsrDI GCAATG_nn'

Cuts at:      0     812     911    2310 
   Size:        812      99    1399

  Fragments arranged by size: 

                1399     812      99
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     311    2310 
   Size:        311    1999
 
BstYI r'GATC_y

Cuts at:      0     162    1077    1554    1574    1994    2310 
   Size:        162     915     477      20     420     316

  Fragments arranged by size: 

                 915     477     420     316     162      20
 
BtgI C'CryG_G

Cuts at:      0    1808    1858    2310 
   Size:       1808      50     452

  Fragments arranged by size: 

                1808     452      50
 
BtsI GCAGTG_nn'

Cuts at:      0      16     316     480    1664    2310 
   Size:         16     300     164    1184     646

  Fragments arranged by size: 

                1184     646     300     164      16
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     795     830    2310 
   Size:        795      35    1480

  Fragments arranged by size: 

                1480     795      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     333    2310 
   Size:        333    1977
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     619    2221    2310 
   Size:        619    1602      89

  Fragments arranged by size: 

                1602     619      89
 
EaeI y'GGCC_r

Cuts at:      0      29    1013    1373    1661    1730    2310 
   Size:         29     984     360     288      69     580

  Fragments arranged by size: 

                 984     580     360     288      69      29
 
EarI CTCTTCn'nnn_

Cuts at:      0     475     761     845     995    1235    2310 
   Size:        475     286      84     150     240    1075

  Fragments arranged by size: 

                1075     475     286     240     150      84
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     647     980    2310 
   Size:        647     333    1330

  Fragments arranged by size: 

                1330     647     333
 
EcoICRI GAG'CTC

Cuts at:      0    1815    2310 
   Size:       1815     495
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     590     956    1438    1681    1837    2021    2188 
   Size:         73     517     366     482     243     156     184     167

Cuts at:   2188    2310 
   Size:        122

  Fragments arranged by size: 

                 517     482     366     243     184     167     156     122
                  73
 
EcoRI G'AATT_C

Cuts at:      0    2020    2310 
   Size:       2020     290
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1207    2310 
   Size:       1207    1103
 
FspI TGC'GCA

Cuts at:      0    1546    2310 
   Size:       1546     764
 
FspAI rTGC'GCAy

Cuts at:      0    1546    2310 
   Size:       1546     764
 
HaeII r_GCGC'y

Cuts at:      0      52    1031    2241    2310 
   Size:         52     979    1210      69

  Fragments arranged by size: 

                1210     979      69      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     893     925    1201    1233    2310 
   Size:        893      32     276      32    1077

  Fragments arranged by size: 

                1077     893     276      32      32
 
HincII GTy'rAC

Cuts at:      0    1125    1973    2214    2310 
   Size:       1125     848     241      96

  Fragments arranged by size: 

                1125     848     241      96
 
KpnI G_GTAC'C

Cuts at:      0      61    2310 
   Size:         61    2249
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2049    2302    2310 
   Size:       2049     253       8

  Fragments arranged by size: 

                2049     253       8
 
MscI TGG'CCA

Cuts at:      0    1375    1732    2310 
   Size:       1375     357     578

  Fragments arranged by size: 

                1375     578     357
 
MslI CAynn'nnrTG

Cuts at:      0     130     134     250    1015    1601    2158    2198    2310 
   Size:        130       4     116     765     586     557      40     112

  Fragments arranged by size: 

                 765     586     557     130     116     112      40       4
            
 
MspA1I CmG'CkG

Cuts at:      0     347     917    1356    2221    2310 
   Size:        347     570     439     865      89

  Fragments arranged by size: 

                 865     570     439     347      89
 
NspI r_CATG'y

Cuts at:      0     129     473     510    1165    1543    2310 
   Size:        129     344      37     655     378     767

  Fragments arranged by size: 

                 767     655     378     344     129      37
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1966    2310 
   Size:       1966     344
 
PfoI T'CCnGG_A

Cuts at:      0    1304    2310 
   Size:       1304    1006
 
PmlI CAC'GTG

Cuts at:      0    1467    2310 
   Size:       1467     843
 
PstI C_TGCA'G

Cuts at:      0     212     242    1442    1659    2310 
   Size:        212      30    1200     217     651

  Fragments arranged by size: 

                1200     651     217     212      30
 
PvuII CAG'CTG

Cuts at:      0    1356    2310 
   Size:       1356     954
 
SacI G_AGCT'C

Cuts at:      0    1817    2310 
   Size:       1817     493
 
ScaI AGT'ACT

Cuts at:      0    1006    2310 
   Size:       1006    1304
 
SexAI A'CCwGG_T

Cuts at:      0    1959    2310 
   Size:       1959     351
 
SfcI C'TryA_G

Cuts at:      0     208     238    1438    1655    2310 
   Size:        208      30    1200     217     655

  Fragments arranged by size: 

                1200     655     217     208      30
 
SmlI C'TyrA_G

Cuts at:      0    1048    1135    1192    2310 
   Size:       1048      87      57    1118

  Fragments arranged by size: 

                1118    1048      87      57
 
StyI C'CwwG_G

Cuts at:      0     194    1750    2156    2310 
   Size:        194    1556     406     154

  Fragments arranged by size: 

                1556     406     194     154
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1807    1955    2310 
   Size:       1807     148     355

  Fragments arranged by size: 

                1807     355     148
 
TatI w'GTAC_w

Cuts at:      0    1004    2180    2310 
   Size:       1004    1176     130

  Fragments arranged by size: 

                1176    1004     130
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     610     642    2102    2134    2310 
   Size:        610      32    1460      32     176

  Fragments arranged by size: 

                1460     610     176      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     843    2310 
   Size:        843    1467
 
XhoI C'TCGA_G

Cuts at:      0    1135    2310 
   Size:       1135    1175
 
ZraI GAC'GTC

Cuts at:      0    1725    2310 
   Size:       1725     585
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI     BglI    BglII Bme1580I     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI   BstAPI    BstYI
     BtgI     BtsI    CspCI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I
   HincII     KpnI     MmeI     MscI     MslI   MspA1I     NspI    PflMI
     PfoI     PmlI     PstI    PvuII     SacI     ScaI    SexAI     SfcI
     SmlI     StyI    TaqII     TatI     TstI     XcmI     XhoI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BciVI     BclI
    BfrBI     BlpI    BmgBI     BmrI     BmtI     BsaI    BsaXI    BseYI
     BsgI    BsiWI    BspEI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI     EagI
    EcoNI EcoO109I    EcoRV     FseI  HindIII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XmaI     XmnI
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