Restriction map for pF1KB3316
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AflIII      Alw44I      AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspLU11I    BsrDI       BssHII      BssSI       BstAPI      
BstEII      BstPI       BstUI       BstXI       Bsu36I      
ClaI        CpoI        DrdI        EaeI        EagI        
EclXI       Eco52I      Eco81I      EcoO65I     EcoT22I     
FauI        FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     KasI        KpnI        KspI        
MamI        MluI        MluNI       MscI        MslI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfcI        SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccIII       1656  TCCGGA               T^CCGGA
AgeI         1834  ACCGGT               A^CCGGT
Aor51HI        61  AGCGCT               AGC^GCT
AspHI        2014  GTGCTC               G[AT]GC[AT]^C
BanI         2098  GGTGCC               G^G[CT][GA]CC
BanII          48  GAGCCC               G[GA]GC[CT]^C
BbsI         1715  GTCTTC               GAAGAC(2/6)
BlpI         2176  GCTCAGC              GC^T[ACGT]AGC
BpmI          707  CTCCAG               CTGGAG(16/14)
Bpu1102I     2176  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1715  GTCTTC               GAAGAC(2/6)
BseAI        1656  TCCGGA               T^CCGGA
BsgI          935  GTGCAG               GTGCAG(16/14)
BsiHKAI      2014  GTGCTC               G[AT]GC[AT]^C
BsmFI        2042  GGGAC                GGGAC(10/14)
Bsp1407I     1209  TGTACA               T^GTACA
BspEI        1656  TCCGGA               T^CCGGA
BspHI        1721  TCATGA               T^CATGA
BspMI        1035  ACCTGC               ACCTGC(4/8)
BsrFI        1834  ACCGGT               [GA]^CCGG[CT]
BsrGI        1209  TGTACA               T^GTACA
Bst1107I     1313  GTATAC               GTA^TAC
BstBI        1667  TTCGAA               TT^CGAA
BstYI         189  AGATCC               [GA]^GATC[CT]
BstZ17I      1313  GTATAC               GTA^TAC
CelII        2176  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1834  ACCGGT               [GA]^CCGG[CT]
DraII        1955  AGGACCT              [GA]G^G[ACGT]CC[CT]
EarI         1010  GAAGAG               CTCTTC(1/4)
Eco47III       61  AGCGCT               AGC^GCT
EcoNI         799  CCTTTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1955  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1932  GAATTC               G^AATTC
EcoRV         358  GATATC               GAT^ATC
HaeII          61  AGCGCT               [GA]GCGC^[CT]
HincII        624  GTTAAC               GT[CT]^[GA]AC
HindII        624  GTTAAC               GT[CT]^[GA]AC
HpaI          624  GTTAAC               GTT^AAC
Ksp632I      1010  GAAGAG               CTCTTC(1/4)
MaeII         354  ACGT                 A^CGT
MfeI         1217  CAATTG               C^AATTG
MflI          189  AGATCC               [GA]^GATC[CT]
MroI         1656  TCCGGA               T^CCGGA
MunI         1217  CAATTG               C^AATTG
NcoI         2102  CCATGG               C^CATGG
NspV         1667  TTCGAA               TT^CGAA
PinAI        1834  ACCGGT               A^CCGGT
PpuMI        1955  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI         1721  TCATGA               T^CATGA
SexAI        1958  ACCTGGT              A^CC[AT]GGT
SfuI         1667  TTCGAA               TT^CGAA
SspBI        1209  TGTACA               T^GTACA
SspI         1881  AATATT               AAT^ATT
StuI         1048  AGGCCT               AGG^CCT
XhoII         189  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         1313  GTATAC               GT^[AC][GT]AC
             1981  GTAGAC              
AfaI          791  GTAC                 GT^AC
             1210  GTAC                
AhdI          748  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1957  GACCTGGTGTC         
AlwNI         266  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1993  CAGAGACTG           
AspEI         748  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1957  GACCTGGTGTC         
BmyI           48  GAGCCC               G[AGT]GC[ACT]^C
             2014  GTGCTC              
BsaI          329  GAGACC               GGTCTC(1/5)
             1370  GAGACC              
BsaWI        1656  TCCGGA               [AT]^CCGG[AT]
             1834  ACCGGT              
BsiYI         799  CCTTTCCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1036  CCTGCAACGGG         
BslI          799  CCTTTCCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1036  CCTGCAACGGG         
Bsp1286I       48  GAGCCC               G[AGT]GC[ACT]^C
             2014  GTGCTC              
BsrBI        2163  CCGCTC               CCGCTC(-3/-3)
             1703  GAGCGG              
DraIII       1118  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1913  CACTCAGTG           
DsaI           25  CCGTGG               C^C[GA][CT]GG
             2102  CCATGG              
Eam1105I      748  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1957  GACCTGGTGTC         
HapII        1657  CCGG                 C^CGG
             1835  CCGG                
HpaII        1657  CCGG                 C^CGG
             1835  CCGG                
MspI         1657  CCGG                 C^CGG
             1835  CCGG                
PvuII          13  CAGCTG               CAG^CTG
             1696  CAGCTG              
RsaI          791  GTAC                 GT^AC
             1210  GTAC                
Tsp45I        614  GTCAC                ^GT[GC]AC
             2139  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
Asp700I     AvaII       BbvI        BfaI        BsaJI       
BseRI       BsmAI       BsmI        BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XmnI        
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