Restriction map for pF1KB3305
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HincII      HindII      HpaI        KpnI        Ksp632I     
MaeI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SalI        SapI        SbfI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII          45  CGCG                 CG^CG
AflIII       1476  ACGTGT               A^C[GA][CT]GT
Alw44I        736  GTGCAC               G^TGCAC
AlwNI        1490  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          748  CCTGAGG              CC^T[ACGT]AGG
ApaI          557  GGGCCC               GGGCC^C
ApaLI         736  GTGCAC               G^TGCAC
BalI          420  TGGCCA               TGG^CCA
BbvCI        1555  GCTGAGG              CCTCAGC(-5/-2)
BglI          500  GCCGAGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          77  AGATCT               A^GATCT
BpmI         1462  CTGGAG               CTGGAG(16/14)
BsaAI        1475  TACGTG               [CT]AC^GT[GA]
BspHI         407  TCATGA               T^CATGA
BstUI          45  CGCG                 CG^CG
BstXI        1634  CCAGTCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        748  CCTGAGG              CC^T[ACGT]AGG
DsaI           44  CCGCGG               C^C[GA][CT]GG
Eco81I        748  CCTGAGG              CC^T[ACGT]AGG
EcoT14I      1445  CCAAGG               C^C[AT][AT]GG
EcoT22I       790  ATGCAT               ATGCA^T
HgaI          867  GCGTC                GACGC(5/10)
HindIII         7  AAGCTT               A^AGCTT
KspI           44  CCGCGG               CCGC^GG
MluNI         420  TGGCCA               TGG^CCA
MscI          420  TGGCCA               TGG^CCA
MseI         1679  TTAA                 T^TAA
MvnI           45  CGCG                 CG^CG
NsiI          790  ATGCAT               ATGCA^T
PleI         1342  GACTC                GAGTC(4/5)
PpuMI         483  GGGACCT              [GA]G^G[AT]CC[CT]
PstI         1078  CTGCAG               CTGCA^G
RcaI          407  TCATGA               T^CATGA
SacII          44  CCGCGG               CCGC^GG
ScaI          285  AGTACT               AGT^ACT
SexAI        1598  ACCAGGT              A^CC[AT]GGT
SmlI         1420  CTCAAG               C^T[CT][GA]AG
SspI          259  AATATT               AAT^ATT
StuI         1046  AGGCCT               AGG^CCT
StyI         1445  CCAAGG               C^C[AT][AT]GG
TfiI         1406  GAATC                G^A[AT]TC
Tru9I        1679  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          245  AAATTC               [GA]^AATT[CT]
             1542  GAATTT              
ApoI          245  AAATTC               [GA]^AATT[CT]
             1542  GAATTT              
AspI          672  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1597  GACCAGGTC           
AvaI          518  CTCGGG               C^[CT]CG[GA]G
             1207  CCCGAG              
BanII         537  GGGCTC               G[GA]GC[CT]^C
              557  GGGCCC              
BbeI          497  GGCGCC               GGCGC^C
             1252  GGCGCC              
BsgI          713  GTGCAG               GTGCAG(16/14)
             1222  GTGCAG              
BsmBI         868  CGTCTC               CGTCTC(1/5)
              352  GAGACG              
BsoBI         518  CTCGGG               C^[CT]CG[GA]G
             1207  CCCGAG              
BspMI         709  GCAGGT               ACCTGC(4/8)
             1224  GCAGGT              
EcoNI         894  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1668  CCTGGCCCAGG         
HinfI        1342  GACTC                G^A[ACGT]TC
             1406  GAATC               
KasI          497  GGCGCC               G^GCGCC
             1252  GGCGCC              
MaeII         689  ACGT                 A^CGT
             1476  ACGT                
NarI          497  GGCGCC               GG^CGCC
             1252  GGCGCC              
PflFI         672  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1597  GACCAGGTC           
SfaNI        1486  GCATC                GCATC(5/9)
             1689  GCATC               
SfoI          497  GGCGCC               GGC^GCC
             1252  GGCGCC              
TaqI          161  TCGA                 T^CGA
              998  TCGA                
Tsp45I         37  GTGAC                ^GT[GC]AC
              370  GTCAC               
Tth111I       672  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1597  GACCAGGTC           
TthHB8I       161  TCGA                 T^CGA
              998  TCGA                
XcmI          608  CCACCCAGTATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1346  CCAAAGTTCGTATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AspHI       AvaII       BanI        BbvI        BcnI        
BmyI        BsaHI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        TseI        Tsp509I     
TspRI       Van91I      XhoII       
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