Restriction map for pF1KB3305
 (Linear) (Six-base) MAPSORT of: KIBB3305.seq  Check: 4746  from: 1  to: 1731



KIBB3305.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     700    1215    1731 
   Size:        700     515     516

  Fragments arranged by size: 

                 700     516     515
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     856     916    1731 
   Size:        466     390      60     815

  Fragments arranged by size: 

                 815     466     390      60
 
AflIII A'CryG_T

Cuts at:      0    1476    1731 
   Size:       1476     255
 
AleI CACnn'nnGTG

Cuts at:      0    1475    1731 
   Size:       1475     256
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1495    1731 
   Size:       1495     236
 
ApaI G_GGCC'C

Cuts at:      0     561    1731 
   Size:        561    1170
 
ApaLI G'TGCA_C

Cuts at:      0     736    1731 
   Size:        736     995
 
ApoI r'AATT_y

Cuts at:      0     245    1542    1731 
   Size:        245    1297     189

  Fragments arranged by size: 

                1297     245     189
 
AvaI C'yCGr_G

Cuts at:      0     518    1207    1731 
   Size:        518     689     524

  Fragments arranged by size: 

                 689     524     518
 
BanI G'GyrC_C

Cuts at:      0     219     497     603     622    1252    1731 
   Size:        219     278     106      19     630     479

  Fragments arranged by size: 

                 630     479     278     219     106      19
 
BanII G_rGCy'C

Cuts at:      0     541     561    1731 
   Size:        541      20    1170

  Fragments arranged by size: 

                1170     541      20
 
BbeI G_GCGC'C

Cuts at:      0     501    1256    1731 
   Size:        501     755     475

  Fragments arranged by size: 

                 755     501     475
 
BbvCI CC'TCA_GC

Cuts at:      0    1556    1731 
   Size:       1556     175
 
BfrBI ATG'CAT

Cuts at:      0     792    1731 
   Size:        792     939
 
BglI GCCn_nnn'nGGC

Cuts at:      0     506    1731 
   Size:        506    1225
 
BglII A'GATC_T

Cuts at:      0      77    1731 
   Size:         77    1654
 
Bme1580I G_kGCm'C

Cuts at:      0     224     561     608     740    1731 
   Size:        224     337      47     132     991

  Fragments arranged by size: 

                 991     337     224     132      47
 
BmgBI CAC'GTC

Cuts at:      0     690    1731 
   Size:        690    1041
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     153     606     669    1181    1731 
   Size:        153     453      63     512     550

  Fragments arranged by size: 

                 550     512     453     153      63
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     774    1731 
   Size:        774     957
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1483    1731 
   Size:       1483     248
 
Bpu10I CC'TnA_GC

Cuts at:      0     803    1397    1556    1731 
   Size:        803     594     159     175

  Fragments arranged by size: 

                 803     594     175     159
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1405    1731 
   Size:       1405     326
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     456    1459    1568    1731 
   Size:        456    1003     109     163

  Fragments arranged by size: 

                1003     456     163     109
 
BsaAI yAC'GTr

Cuts at:      0    1477    1731 
   Size:       1477     254
 
BsaHI Gr'CG_yC

Cuts at:      0     498     867    1253    1731 
   Size:        498     369     386     478

  Fragments arranged by size: 

                 498     478     386     369
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      46      64      76      94     257     287    1731 
   Size:         46      18      12      18     163      30    1444

  Fragments arranged by size: 

                1444     163      46      30      18      18      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     750     927    1441    1731 
   Size:        750     177     514     290

  Fragments arranged by size: 

                 750     514     290     177
 
BseYI C'CCAG_C

Cuts at:      0     269     561    1128    1159    1731 
   Size:        269     292     567      31     572

  Fragments arranged by size: 

                 572     567     292     269      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     734    1243    1731 
   Size:        734     509     488

  Fragments arranged by size: 

                 734     509     488
 
BsiHKAI G_wGCw'C

Cuts at:      0      74     143     740    1731 
   Size:         74      69     597     991

  Fragments arranged by size: 

                 991     597      74      69
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     346     874    1731 
   Size:        346     528     857

  Fragments arranged by size: 

                 857     528     346
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     143     224     541     561     608     740    1731 
   Size:         74      69      81     317      20      47     132     991

  Fragments arranged by size: 

                 991     317     132      81      74      69      47      20
            
 
BspHI T'CATG_A

Cuts at:      0     407    1731 
   Size:        407    1324
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     700    1215    1731 
   Size:        700     515     516

  Fragments arranged by size: 

                 700     516     515
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1641    1731 
   Size:       1641      90
 
BstYI r'GATC_y

Cuts at:      0      77     839    1655    1731 
   Size:         77     762     816      76

  Fragments arranged by size: 

                 816     762      77      76
 
Bsu36I CC'TnA_GG

Cuts at:      0     749    1731 
   Size:        749     982
 
BtgI C'CryG_G

Cuts at:      0      44    1731 
   Size:         44    1687
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     163    1731 
   Size:        163    1568
 
BtsI GCAGTG_nn'

Cuts at:      0     365    1253    1731 
   Size:        365     888     478

  Fragments arranged by size: 

                 888     478     365
 
EaeI y'GGCC_r

Cuts at:      0      90     420    1497    1731 
   Size:         90     330    1077     234

  Fragments arranged by size: 

                1077     330     234      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     856     916    1483    1731 
   Size:        466     390      60     567     248

  Fragments arranged by size: 

                 567     466     390     248      60
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     898    1672    1731 
   Size:        898     774      59

  Fragments arranged by size: 

                 898     774      59
 
EcoO109I rG'GnC_Cy

Cuts at:      0     484     557     558    1731 
   Size:        484      73       1    1173

  Fragments arranged by size: 

                1173     484      73       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     795    1731 
   Size:        795     936
 
HaeII r_GCGC'y

Cuts at:      0     501    1256    1310    1731 
   Size:        501     755      54     421

  Fragments arranged by size: 

                 755     501     421      54
 
HindIII A'AGCT_T

Cuts at:      0       7    1731 
   Size:          7    1724
 
KasI G'GCGC_C

Cuts at:      0     497    1252    1731 
   Size:        497     755     479

  Fragments arranged by size: 

                 755     497     479
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     410    1731 
   Size:        404       6    1321

  Fragments arranged by size: 

                1321     404       6
 
MscI TGG'CCA

Cuts at:      0     422    1731 
   Size:        422    1309
 
MslI CAynn'nnrTG

Cuts at:      0     733    1475    1477    1628    1731 
   Size:        733     742       2     151     103

  Fragments arranged by size: 

                 742     733     151     103       2
 
MspA1I CmG'CkG

Cuts at:      0      46      89     553     718    1495    1555    1731 
   Size:         46      43     464     165     777      60     176

  Fragments arranged by size: 

                 777     464     176     165      60      46      43
 
NarI GG'CG_CC

Cuts at:      0     498    1253    1731 
   Size:        498     755     478

  Fragments arranged by size: 

                 755     498     478
 
NsiI A_TGCA'T

Cuts at:      0     794    1731 
   Size:        794     937
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      99     429     618    1248    1731 
   Size:         99     330     189     630     483

  Fragments arranged by size: 

                 630     483     330     189      99
 
PfoI T'CCnGG_A

Cuts at:      0     172    1731 
   Size:        172    1559
 
PpuMI rG'GwC_Cy

Cuts at:      0     484    1731 
   Size:        484    1247
 
PspOMI G'GGCC_C

Cuts at:      0     557    1731 
   Size:        557    1174
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      27    1731 
   Size:         27    1704
 
PstI C_TGCA'G

Cuts at:      0    1082    1731 
   Size:       1082     649
 
PvuII CAG'CTG

Cuts at:      0     718    1495    1555    1731 
   Size:        718     777      60     176

  Fragments arranged by size: 

                 777     718     176      60
 
SacII CC_GC'GG

Cuts at:      0      47    1731 
   Size:         47    1684
 
ScaI AGT'ACT

Cuts at:      0     287    1731 
   Size:        287    1444
 
SexAI A'CCwGG_T

Cuts at:      0    1598    1731 
   Size:       1598     133
 
SfcI C'TryA_G

Cuts at:      0      84    1078    1115    1185    1731 
   Size:         84     994      37      70     546

  Fragments arranged by size: 

                 994     546      84      70      37
 
SfoI GGC'GCC

Cuts at:      0     499    1254    1731 
   Size:        499     755     477

  Fragments arranged by size: 

                 755     499     477
 
SmlI C'TyrA_G

Cuts at:      0    1420    1731 
   Size:       1420     311
 
SspI AAT'ATT

Cuts at:      0     261    1731 
   Size:        261    1470
 
StuI AGG'CCT

Cuts at:      0    1048    1731 
   Size:       1048     683
 
StyI C'CwwG_G

Cuts at:      0    1445    1731 
   Size:       1445     286
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     610     625    1731 
   Size:        610      15    1106

  Fragments arranged by size: 

                1106     610      15
 
TatI w'GTAC_w

Cuts at:      0     285    1731 
   Size:        285    1446
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      64      96     257     289    1511    1543    1731 
   Size:         64      32     161      32    1222      32     188

  Fragments arranged by size: 

                1222     188     161      64      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     675    1600    1731 
   Size:        675     925     131

  Fragments arranged by size: 

                 925     675     131
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     615    1353    1731 
   Size:        615     738     378

  Fragments arranged by size: 

                 738     615     378
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI     ApoI
     AvaI     BanI    BanII     BbeI    BbvCI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     EaeI  Eco57MI    EcoNI EcoO109I     FalI    HaeII  HindIII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NsiI    PflMI
     PfoI    PpuMI   PspOMI     PsrI     PstI    PvuII    SacII     ScaI
    SexAI     SfcI     SfoI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI     BcgI    BciVI     BclI     BlpI     BmtI    BsaBI    BsaWI
    BsiEI    BsiWI     BsmI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KpnI
     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PvuI    RsrII     SacI     SalI    SanDI
     SapI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI     XbaI     XhoI     XmaI     XmnI     ZraI
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