Restriction map for pF1KB3279
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AgeI        AhdI        AocI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbvCI       BcgI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsiWI       BsmBI       
BspDI       BspHI       BspLU11I    BstAPI      BstBI       
Bsu36I      CelII       ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoT14I     EcoT22I     FseI        
FspI        HindIII     HpaI        KpnI        Ksp632I     
MamI        MfeI        MunI        NcoI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SnaBI       
SpeI        Sse8387I    SspI        StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        615  TCCGGA               T^CCGGA
AclI          760  AACGTT               AA^CGTT
AflII         891  CTTAAG               C^TTAAG
AlwNI         148  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2061  GGGCCC               GGGCC^C
Asp700I       414  GAATCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         2515  TGGCCA               TGG^CCA
BbsI         2295  GAAGAC               GAAGAC(2/6)
BciVI        2524  GTATCC               GTATCC(6/5)
BfrI          891  CTTAAG               C^TTAAG
BpuAI        2295  GAAGAC               GAAGAC(2/6)
BsaAI        1519  TACGTG               [CT]AC^GT[GA]
BsaI         2761  GAGACC               GGTCTC(1/5)
BseAI         615  TCCGGA               T^CCGGA
BsgI          303  GTGCAG               GTGCAG(16/14)
BsmAI        2761  GAGAC                GTCTC(1/5)
BsmI         2477  GCATTC               GAATGC(1/-1)
BspEI         615  TCCGGA               T^CCGGA
BsrDI          64  GCAATG               GCAATG(2/0)
BssSI        1573  CACGAG               CACGAG(-5/-1)
Bst1107I      738  GTATAC               GTA^TAC
BstXI        1988  CCACTGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       738  GTATAC               GTA^TAC
DraI         2605  TTTAAA               TTT^AAA
EcoRI         258  GAATTC               G^AATTC
EcoRV         481  GATATC               GAT^ATC
KspI          210  CCGCGG               CCGC^GG
MluNI        2515  TGGCCA               TGG^CCA
MroI          615  TCCGGA               T^CCGGA
MscI         2515  TGGCCA               TGG^CCA
NdeI          919  CATATG               CA^TATG
NspI         2444  GCATGC               [GA]CATG^[CT]
Psp1406I      760  AACGTT               AA^CGTT
PvuII        2595  CAGCTG               CAG^CTG
SacI         1232  GAGCTC               GAGCT^C
SacII         210  CCGCGG               CCGC^GG
SmaI         2319  CCCGGG               CCC^GGG
SphI         2444  GCATGC               GCATG^C
XmaCI        2319  CCCGGG               C^CCGGG
XmaI         2319  CCCGGG               C^CCGGG
XmnI          414  GAATCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          328  GTAGAC               GT^[AC][GT]AC
              738  GTATAC              
AcsI          258  GAATTC               [GA]^AATT[CT]
             2659  AAATTC              
Alw44I       1528  GTGCAC               G^TGCAC
             2458  GTGCAC              
Aor51HI      1502  AGCGCT               AGC^GCT
             1971  AGCGCT              
ApaLI        1528  GTGCAC               G^TGCAC
             2458  GTGCAC              
ApoI          258  GAATTC               [GA]^AATT[CT]
             2659  AAATTC              
BbeI         1756  GGCGCC               GGCGC^C
             2022  GGCGCC              
BclI         2099  TGATCA               T^GATCA
             2505  TGATCA              
BsaWI         615  TCCGGA               [AT]^CCGG[AT]
             2635  TCCGGT              
BsiEI         270  CGACCG               CG[GA][CT]^CG
             1747  CGGTCG              
BsrFI        1686  GCCGGC               [GA]^CCGG[CT]
             1759  GCCGGC              
BstEII       1152  GGTGACC              G^GT[ACGT]ACC
             1272  GGTTACC             
BstPI        1152  GGTGACC              G^GT[ACGT]ACC
             1272  GGTTACC             
Cfr10I       1686  GCCGGC               [GA]^CCGG[CT]
             1759  GCCGGC              
Eco47III     1502  AGCGCT               AGC^GCT
             1971  AGCGCT              
EcoNI         219  CCTTCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2463  CCTAGAGGAGG         
EcoO65I      1152  GGTGACC              G^GT[ACGT]ACC
             1272  GGTTACC             
FbaI         2099  TGATCA               T^GATCA
             2505  TGATCA              
KasI         1756  GGCGCC               G^GCGCC
             2022  GGCGCC              
MluI         1811  ACGCGT               A^CGCGT
             1900  ACGCGT              
MslI          126  CACCTTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              787  CACCCCAATG          
NaeI         1686  GCCGGC               GCC^GGC
             1759  GCCGGC              
NarI         1756  GGCGCC               GG^CGCC
             2022  GGCGCC              
NgoMIV       1686  GCCGGC               G^CCGGC
             1759  GCCGGC              
PleI          445  GACTC                GAGTC(4/5)
             1798  GACTC               
PpuMI         149  AGGACCT              [GA]G^G[AT]CC[CT]
              215  GGGACCT             
SfoI         1756  GGCGCC               GGC^GCC
             2022  GGCGCC              
SgrAI        1685  CGCCGGCG             C[GA]^CCGG[CT]G
             1758  CGCCGGCG            
SmlI            9  CTCAAG               C^T[CT][GA]AG
              891  CTTAAG              
XcmI          872  CCAAGTTCCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1316  CCAGGGTGTCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AspHI       AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspMI       BsrBI       BsrGI       
BsrI        BssHII      BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SspBI       TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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