Restriction map for pF1KB3279
 (Linear) (Six-base) MAPSORT of: KIBB3279.seq  Check: 8165  from: 1  to: 2850



KIBB3279.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1140    1644    2850 
   Size:       1140     504    1206

  Fragments arranged by size: 

                1206    1140     504
 
AccI GT'mk_AC

Cuts at:      0     329     739    2850 
   Size:        329     410    2111

  Fragments arranged by size: 

                2111     410     329
 
AclI AA'CG_TT

Cuts at:      0     761    2850 
   Size:        761    2089
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2298    2850 
   Size:       2298     552
 
AfeI AGC'GCT

Cuts at:      0    1504    1973    2850 
   Size:       1504     469     877

  Fragments arranged by size: 

                1504     877     469
 
AflII C'TTAA_G

Cuts at:      0     891    2850 
   Size:        891    1959
 
AflIII A'CryG_T

Cuts at:      0    1520    1811    1900    2090    2850 
   Size:       1520     291      89     190     760

  Fragments arranged by size: 

                1520     760     291     190      89
 
AleI CACnn'nnGTG

Cuts at:      0     130    2850 
   Size:        130    2720
 
AlwNI CAG_nnn'CTG

Cuts at:      0     153    2850 
   Size:        153    2697
 
ApaI G_GGCC'C

Cuts at:      0    2065    2850 
   Size:       2065     785
 
ApaLI G'TGCA_C

Cuts at:      0    1528    2458    2850 
   Size:       1528     930     392

  Fragments arranged by size: 

                1528     930     392
 
ApoI r'AATT_y

Cuts at:      0     258    2659    2850 
   Size:        258    2401     191

  Fragments arranged by size: 

                2401     258     191
 
AvaI C'yCGr_G

Cuts at:      0     658    1800    2065    2319    2850 
   Size:        658    1142     265     254     531

  Fragments arranged by size: 

                1142     658     531     265     254
 
BanI G'GyrC_C

Cuts at:      0     124     177     670    1756    2022    2053    2850 
   Size:        124      53     493    1086     266      31     797

  Fragments arranged by size: 

                1086     797     493     266     124      53      31
 
BanII G_rGCy'C

Cuts at:      0     520    1236    1294    2065    2850 
   Size:        520     716      58     771     785

  Fragments arranged by size: 

                 785     771     716     520      58
 
BbeI G_GCGC'C

Cuts at:      0    1760    2026    2850 
   Size:       1760     266     824

  Fragments arranged by size: 

                1760     824     266
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2302    2850 
   Size:       2302     548
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2535    2850 
   Size:       2535     315
 
BclI T'GATC_A

Cuts at:      0    2099    2505    2850 
   Size:       2099     406     345

  Fragments arranged by size: 

                2099     406     345
 
Bme1580I G_kGCm'C

Cuts at:      0    1532    1717    1908    2065    2462    2850 
   Size:       1532     185     191     157     397     388

  Fragments arranged by size: 

                1532     397     388     191     185     157
 
BmgBI CAC'GTC

Cuts at:      0     360     543    1176    1341    2850 
   Size:        360     183     633     165    1509

  Fragments arranged by size: 

                1509     633     360     183     165
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     712     949    1102    2396    2850 
   Size:        712     237     153    1294     454

  Fragments arranged by size: 

                1294     712     454     237     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     244     337     374     828     842    1603    2552 
   Size:        181      63      93      37     454      14     761     949

Cuts at:   2552    2850 
   Size:        298

  Fragments arranged by size: 

                 949     761     454     298     181      93      63      37
                  14
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2755    2850 
   Size:       2755      95
 
BsaAI yAC'GTr

Cuts at:      0    1521    2850 
   Size:       1521    1329
 
BsaHI Gr'CG_yC

Cuts at:      0    1168    1757    1793    2023    2044    2850 
   Size:       1168     589      36     230      21     806

  Fragments arranged by size: 

                1168     806     589     230      36      21
 
BsaWI w'CCGG_w

Cuts at:      0     615    2635    2850 
   Size:        615    2020     215

  Fragments arranged by size: 

                2020     615     215
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     826     856    1477    1507    2413    2443    2850 
   Size:        826      30     621      30     906      30     407

  Fragments arranged by size: 

                 906     826     621     407      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      32     292    1591    2274    2482    2773    2850 
   Size:         32     260    1299     683     208     291      77

  Fragments arranged by size: 

                1299     683     291     260     208      77      32
 
BseYI C'CCAG_C

Cuts at:      0     399    2850 
   Size:        399    2451
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324    2850 
   Size:        324    2526
 
BsiEI CG_ry'CG

Cuts at:      0     273    1750    2850 
   Size:        273    1477    1100

  Fragments arranged by size: 

                1477    1100     273
 
BsiHKAI G_wGCw'C

Cuts at:      0     945    1236    1532    2170    2278    2462    2850 
   Size:        945     291     296     638     108     184     388

  Fragments arranged by size: 

                 945     638     388     296     291     184     108
 
BsmI GAATG_Cn'

Cuts at:      0    2477    2850 
   Size:       2477     373
 
Bsp1286I G_dGCh'C

Cuts at:      0     520     945    1236    1294    1532    1717    1908    2065 
   Size:        520     425     291      58     238     185     191     157

Cuts at:   2065    2170    2278    2462    2850 
   Size:        105     108     184     388

  Fragments arranged by size: 

                 520     425     388     291     238     191     185     184
                 157     108     105      58
 
BspEI T'CCGG_A

Cuts at:      0     615    2850 
   Size:        615    2235
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     333     552    1140    1165    1644    2850 
   Size:        333     219     588      25     479    1206

  Fragments arranged by size: 

                1206     588     479     333     219      25
 
BsrBI CCG'CTC

Cuts at:      0      83    1488    1541    2015    2850 
   Size:         83    1405      53     474     835

  Fragments arranged by size: 

                1405     835     474      83      53
 
BsrDI GCAATG_nn'

Cuts at:      0      71    2850 
   Size:         71    2779
 
BsrFI r'CCGG_y

Cuts at:      0    1686    1759    2850 
   Size:       1686      73    1091

  Fragments arranged by size: 

                1686    1091      73
 
BsrGI T'GTAC_A

Cuts at:      0    1000    1877    2150    2850 
   Size:       1000     877     273     700

  Fragments arranged by size: 

                1000     877     700     273
 
BssHII G'CGCG_C

Cuts at:      0    1607    1609    1782    2195    2850 
   Size:       1607       2     173     413     655

  Fragments arranged by size: 

                1607     655     413     173       2
 
BssSI C'ACGA_G

Cuts at:      0    1573    2850 
   Size:       1573    1277
 
BstEII G'GTnAC_C

Cuts at:      0    1152    1272    2850 
   Size:       1152     120    1578

  Fragments arranged by size: 

                1578    1152     120
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1995    2850 
   Size:       1995     855
 
BstYI r'GATC_y

Cuts at:      0      21     950    2341    2674    2850 
   Size:         21     929    1391     333     176

  Fragments arranged by size: 

                1391     929     333     176      21
 
BstZ17I GTA'TAC

Cuts at:      0     740    2850 
   Size:        740    2110
 
BtgI C'CryG_G

Cuts at:      0     210    1310    1959    1982    2057    2850 
   Size:        210    1100     649      23      75     793

  Fragments arranged by size: 

                1100     793     649     210      75      23
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     126     408    2088    2188    2850 
   Size:        126     282    1680     100     662

  Fragments arranged by size: 

                1680     662     282     126     100
 
BtsI GCAGTG_nn'

Cuts at:      0    1733    1793    2518    2850 
   Size:       1733      60     725     332

  Fragments arranged by size: 

                1733     725     332      60
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      53      88    2850 
   Size:         53      35    2762

  Fragments arranged by size: 

                2762      53      35
 
DraI TTT'AAA

Cuts at:      0    2607    2850 
   Size:       2607     243
 
EaeI y'GGCC_r

Cuts at:      0      84     638    1313    1334    1844    1979    1985    2016 
   Size:         84     554     675      21     510     135       6      31

Cuts at:   2016    2515    2850 
   Size:        499     335

  Fragments arranged by size: 

                 675     554     510     499     335     135      84      31
                  21       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     184     306    1245    2850 
   Size:        184     122     939    1605

  Fragments arranged by size: 

                1605     939     184     122
 
EcoICRI GAG'CTC

Cuts at:      0    1234    2850 
   Size:       1234    1616
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     244     337     374     828     842    1603    2298 
   Size:        181      63      93      37     454      14     761     695

Cuts at:   2298    2552    2850 
   Size:        254     298

  Fragments arranged by size: 

                 761     695     454     298     254     181      93      63
                  37      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     223    2467    2850 
   Size:        223    2244     383

  Fragments arranged by size: 

                2244     383     223
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     216    2062    2500    2850 
   Size:        150      66    1846     438     350

  Fragments arranged by size: 

                1846     438     350     150      66
 
EcoRI G'AATT_C

Cuts at:      0     258    2850 
   Size:        258    2592
 
EcoRV GAT'ATC

Cuts at:      0     483    2850 
   Size:        483    2367
 
HaeII r_GCGC'y

Cuts at:      0    1506    1693    1760    1938    1975    2026    2850 
   Size:       1506     187      67     178      37      51     824

  Fragments arranged by size: 

                1506     824     187     178      67      51      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     853     885    1237    1269    1276    1308 
   Size:        454      32     367      32     352      32       7      32

Cuts at:   1308    1783    1815    2850 
   Size:        475      32    1035

  Fragments arranged by size: 

                1035     475     454     367     352      32      32      32
                  32      32       7
 
HincII GTy'rAC

Cuts at:      0    1049    2088    2586    2850 
   Size:       1049    1039     498     264

  Fragments arranged by size: 

                1049    1039     498     264
 
KasI G'GCGC_C

Cuts at:      0    1756    2022    2850 
   Size:       1756     266     828

  Fragments arranged by size: 

                1756     828     266
 
MluI A'CGCG_T

Cuts at:      0    1811    1900    2850 
   Size:       1811      89     950

  Fragments arranged by size: 

                1811     950      89
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     176    1547    2850 
   Size:        176    1371    1303

  Fragments arranged by size: 

                1371    1303     176
 
MscI TGG'CCA

Cuts at:      0    2517    2850 
   Size:       2517     333
 
MslI CAynn'nnrTG

Cuts at:      0     130     791    2850 
   Size:        130     661    2059

  Fragments arranged by size: 

                2059     661     130
 
MspA1I CmG'CkG

Cuts at:      0     212     339    2597    2850 
   Size:        212     127    2258     253

  Fragments arranged by size: 

                2258     253     212     127
 
NaeI GCC'GGC

Cuts at:      0    1688    1761    2850 
   Size:       1688      73    1089

  Fragments arranged by size: 

                1688    1089      73
 
NarI GG'CG_CC

Cuts at:      0    1757    2023    2850 
   Size:       1757     266     827

  Fragments arranged by size: 

                1757     827     266
 
NdeI CA'TA_TG

Cuts at:      0     920    2850 
   Size:        920    1930
 
NgoMIV G'CCGG_C

Cuts at:      0    1686    1759    2850 
   Size:       1686      73    1091

  Fragments arranged by size: 

                1686    1091      73
 
NspI r_CATG'y

Cuts at:      0    2448    2850 
   Size:       2448     402
 
PfoI T'CCnGG_A

Cuts at:      0     104     536    2493    2850 
   Size:        104     432    1957     357

  Fragments arranged by size: 

                1957     432     357     104
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     216    2850 
   Size:        150      66    2634

  Fragments arranged by size: 

                2634     150      66
 
PspOMI G'GGCC_C

Cuts at:      0    2061    2850 
   Size:       2061     789
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     723     755    1369    1401    2850 
   Size:        723      32     614      32    1449

  Fragments arranged by size: 

                1449     723     614      32      32
 
PstI C_TGCA'G

Cuts at:      0     239     344    1562    1655    1835    2850 
   Size:        239     105    1218      93     180    1015

  Fragments arranged by size: 

                1218    1015     239     180     105      93
 
PvuII CAG'CTG

Cuts at:      0    2597    2850 
   Size:       2597     253
 
SacI G_AGCT'C

Cuts at:      0    1236    2850 
   Size:       1236    1614
 
SacII CC_GC'GG

Cuts at:      0     213    2850 
   Size:        213    2637
 
SfcI C'TryA_G

Cuts at:      0     235     340    1558    1591    1651    1831    2345    2850 
   Size:        235     105    1218      33      60     180     514     505

  Fragments arranged by size: 

                1218     514     505     235     180     105      60      33
            
 
SfoI GGC'GCC

Cuts at:      0    1758    2024    2850 
   Size:       1758     266     826

  Fragments arranged by size: 

                1758     826     266
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1686    1759    2850 
   Size:       1686      73    1091

  Fragments arranged by size: 

                1686    1091      73
 
SmaI CCC'GGG

Cuts at:      0    2321    2850 
   Size:       2321     529
 
SmlI C'TyrA_G

Cuts at:      0       9     891    2850 
   Size:          9     882    1959

  Fragments arranged by size: 

                1959     882       9
 
SphI G_CATG'C

Cuts at:      0    2448    2850 
   Size:       2448     402
 
SrfI GCCC'GGGC

Cuts at:      0    2321    2850 
   Size:       2321     529
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      60    2041    2314    2850 
   Size:         60    1981     273     536

  Fragments arranged by size: 

                1981     536     273      60
 
TatI w'GTAC_w

Cuts at:      0    1000    1376    1877    2150    2850 
   Size:       1000     376     501     273     700

  Fragments arranged by size: 

                1000     700     501     376     273
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     764     796     935     967    2850 
   Size:        764      32     139      32    1883

  Fragments arranged by size: 

                1883     764     139      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     879    1323    2850 
   Size:        879     444    1527

  Fragments arranged by size: 

                1527     879     444
 
XmaI C'CCGG_G

Cuts at:      0    2319    2850 
   Size:       2319     531
 
XmnI GAAnn'nnTTC

Cuts at:      0     418    2850 
   Size:        418    2432
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI    AflII   AflIII     AleI
    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BciVI     BclI Bme1580I    BmgBI     BmrI     BpmI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI    CspCI     DraI     EaeI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII     KasI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NspI     PfoI    PpuMI   PspOMI     PsrI     PstI    PvuII
     SacI    SacII     SfcI     SfoI    SgrAI     SmaI     SmlI     SphI
     SrfI    TaqII     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AgeI     AhdI     AlfI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BfrBI     BglI
    BglII     BlpI     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsiWI
    BsmBI    BspHI   BstAPI    BstBI   Bsu36I     ClaI   DraIII     DrdI
     EagI     EarI     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     NcoI     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SnaBI     SpeI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XhoI     ZraI
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