Restriction map for pF1KB3272
 (Linear) (Six-base) MAPSORT of: KIBB3272.seq  Check: 3475  from: 1  to: 3795



KIBB3272.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     141    1871    3795 
   Size:        141    1730    1924

  Fragments arranged by size: 

                1924    1730     141
 
AatII G_ACGT'C

Cuts at:      0    1154    2264    3795 
   Size:       1154    1110    1531

  Fragments arranged by size: 

                1531    1154    1110
 
AccI GT'mk_AC

Cuts at:      0    2804    2915    2954    3795 
   Size:       2804     111      39     841

  Fragments arranged by size: 

                2804     841     111      39
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1497    1803    2034    2944    3520    3795 
   Size:       1497     306     231     910     576     275

  Fragments arranged by size: 

                1497     910     576     306     275     231
 
AfeI AGC'GCT

Cuts at:      0    2224    3795 
   Size:       2224    1571
 
AflIII A'CryG_T

Cuts at:      0     983    1445    3795 
   Size:        983     462    2350

  Fragments arranged by size: 

                2350     983     462
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1579    1611    3795 
   Size:       1579      32    2184

  Fragments arranged by size: 

                2184    1579      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     783     902    3795 
   Size:        783     119    2893

  Fragments arranged by size: 

                2893     783     119
 
ApaI G_GGCC'C

Cuts at:      0    1091    1378    3795 
   Size:       1091     287    2417

  Fragments arranged by size: 

                2417    1091     287
 
ApoI r'AATT_y

Cuts at:      0     658    3368    3795 
   Size:        658    2710     427

  Fragments arranged by size: 

                2710     658     427
 
AvaI C'yCGr_G

Cuts at:      0     112    1188    1379    1991    2209    2407    3157    3795 
   Size:        112    1076     191     612     218     198     750     638

  Fragments arranged by size: 

                1076     750     638     612     218     198     191     112
            
 
BamHI G'GATC_C

Cuts at:      0    1760    2280    2441    3795 
   Size:       1760     520     161    1354

  Fragments arranged by size: 

                1760    1354     520     161
 
BanI G'GyrC_C

Cuts at:      0    1675    2377    3795 
   Size:       1675     702    1418

  Fragments arranged by size: 

                1675    1418     702
 
BanII G_rGCy'C

Cuts at:      0      63     590     777    1091    1378    2147    2216    2408 
   Size:         63     527     187     314     287     769      69     192

Cuts at:   2408    2633    2687    2789    3342    3795 
   Size:        225      54     102     553     453

  Fragments arranged by size: 

                 769     553     527     453     314     287     225     192
                 187     102      69      63      54
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     281     505    2528    2610    3795 
   Size:        281     224    2023      82    1185

  Fragments arranged by size: 

                2023    1185     281     224      82
 
BbvCI CC'TCA_GC

Cuts at:      0     316    3325    3607    3795 
   Size:        316    3009     282     188

  Fragments arranged by size: 

                3009     316     282     188
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     291     325    2020    2054    3795 
   Size:        291      34    1695      34    1741

  Fragments arranged by size: 

                1741    1695     291      34      34
 
BclI T'GATC_A

Cuts at:      0     134     515    3795 
   Size:        134     381    3280

  Fragments arranged by size: 

                3280     381     134
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1382    3795 
   Size:       1382    2413
 
BglII A'GATC_T

Cuts at:      0    3263    3795 
   Size:       3263     532
 
BlpI GC'TnA_GC

Cuts at:      0     325     589     842    2362    3795 
   Size:        325     264     253    1520    1433

  Fragments arranged by size: 

                1520    1433     325     264     253
 
Bme1580I G_kGCm'C

Cuts at:      0    1091    1378    1553    1907    2382    3795 
   Size:       1091     287     175     354     475    1413

  Fragments arranged by size: 

                1413    1091     475     354     287     175
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1192    2279    2766    3795 
   Size:       1192    1087     487    1029

  Fragments arranged by size: 

                1192    1087    1029     487
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2496    3795 
   Size:       2496    1299
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     180    2239    2647    3589    3709    3795 
   Size:         85      95    2059     408     942     120      86

  Fragments arranged by size: 

                2059     942     408     120      95      86      85
 
Bpu10I CC'TnA_GC

Cuts at:      0     316     776    1951    3325    3607    3795 
   Size:        316     460    1175    1374     282     188

  Fragments arranged by size: 

                1374    1175     460     316     282     188
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1693    2543    3795 
   Size:       1693     850    1252

  Fragments arranged by size: 

                1693    1252     850
 
BsaAI yAC'GTr

Cuts at:      0     875     986    3795 
   Size:        875     111    2809

  Fragments arranged by size: 

                2809     875     111
 
BsaBI GATnn'nnATC

Cuts at:      0    1354    3795 
   Size:       1354    2441
 
BsaHI Gr'CG_yC

Cuts at:      0    1151    2261    3795 
   Size:       1151    1110    1534

  Fragments arranged by size: 

                1534    1151    1110
 
BsaWI w'CCGG_w

Cuts at:      0    2012    2652    3795 
   Size:       2012     640    1143

  Fragments arranged by size: 

                2012    1143     640
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     952     982    1579    1609    2380    2410    3795 
   Size:        952      30     597      30     771      30    1385

  Fragments arranged by size: 

                1385     952     771     597      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     960    1231    1555    2512    2695    3582    3624    3795 
   Size:        960     271     324     957     183     887      42     171

  Fragments arranged by size: 

                 960     957     887     324     271     183     171      42
            
 
BseYI C'CCAG_C

Cuts at:      0      69    1079    1330    1371    2109    3795 
   Size:         69    1010     251      41     738    1686

  Fragments arranged by size: 

                1686    1010     738     251      69      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1177    3795 
   Size:       1177    2618
 
BsiEI CG_ry'CG

Cuts at:      0    2097    3795 
   Size:       2097    1698
 
BsiHKAI G_wGCw'C

Cuts at:      0     576     590    1223    2147    2216    2687    2789    3210 
   Size:        576      14     633     924      69     471     102     421

Cuts at:   3210    3795 
   Size:        585

  Fragments arranged by size: 

                 924     633     585     576     471     421     102      69
                  14
 
BsmI GAATG_Cn'

Cuts at:      0     408    2166    3795 
   Size:        408    1758    1629

  Fragments arranged by size: 

                1758    1629     408
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     136    1651    1849    2872    3795 
   Size:        136    1515     198    1023     923

  Fragments arranged by size: 

                1515    1023     923     198     136
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     576     590     777    1091    1223    1378    1553 
   Size:         63     513      14     187     314     132     155     175

Cuts at:   1553    1907    2147    2216    2382    2408    2633    2687    2789 
   Size:        354     240      69     166      26     225      54     102

Cuts at:   2789    3210    3342    3795 
   Size:        421     132     453

  Fragments arranged by size: 

                 513     453     421     354     314     240     225     187
                 175     166     155     132     132     102      69      63
                  54      26      14
 
BspEI T'CCGG_A

Cuts at:      0    2012    3795 
   Size:       2012    1783
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     141     734    1289    1871    3795 
   Size:        141     593     555     582    1924

  Fragments arranged by size: 

                1924     593     582     555     141
 
BsrBI CCG'CTC

Cuts at:      0     117     801    1766    2867    3795 
   Size:        117     684     965    1101     928

  Fragments arranged by size: 

                1101     965     928     684     117
 
BsrDI GCAATG_nn'

Cuts at:      0    1490    3678    3795 
   Size:       1490    2188     117

  Fragments arranged by size: 

                2188    1490     117
 
BsrFI r'CCGG_y

Cuts at:      0    1101    2096    3795 
   Size:       1101     995    1699

  Fragments arranged by size: 

                1699    1101     995
 
BsrGI T'GTAC_A

Cuts at:      0    1442    3795 
   Size:       1442    2353
 
BssSI C'ACGA_G

Cuts at:      0     887    1921    3795 
   Size:        887    1034    1874

  Fragments arranged by size: 

                1874    1034     887
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2106    3795 
   Size:       2106    1689
 
BstEII G'GTnAC_C

Cuts at:      0     997    3795 
   Size:        997    2798
 
BstYI r'GATC_y

Cuts at:      0     780     965    1760    2280    2441    3173    3263    3795 
   Size:        780     185     795     520     161     732      90     532

  Fragments arranged by size: 

                 795     780     732     532     520     185     161      90
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    2532    2591    3795 
   Size:       2532      59    1204

  Fragments arranged by size: 

                2532    1204      59
 
BtgI C'CryG_G

Cuts at:      0       8    1113    1298    1772    1933    1956    2373    3795 
   Size:          8    1105     185     474     161      23     417    1422

  Fragments arranged by size: 

                1422    1105     474     417     185     161      23       8
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     412    1400    2369    2760    3795 
   Size:        412     988     969     391    1035

  Fragments arranged by size: 

                1035     988     969     412     391
 
BtsI GCAGTG_nn'

Cuts at:      0     540    1221    1752    3795 
   Size:        540     681     531    2043

  Fragments arranged by size: 

                2043     681     540     531
 
ClaI AT'CG_AT

Cuts at:      0     646    3795 
   Size:        646    3149
 
DraI TTT'AAA

Cuts at:      0     525    3795 
   Size:        525    3270
 
DraIII CAC_nnn'GTG

Cuts at:      0    2106    3795 
   Size:       2106    1689
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2800    3795 
   Size:       2800     995
 
EaeI y'GGCC_r

Cuts at:      0    1250    2094    3559    3795 
   Size:       1250     844    1465     236

  Fragments arranged by size: 

                1465    1250     844     236
 
EagI C'GGCC_G

Cuts at:      0    2094    3795 
   Size:       2094    1701
 
EarI CTCTTCn'nnn_

Cuts at:      0      37     616    1609    2985    3572    3760    3795 
   Size:         37     579     993    1376     587     188      35

  Fragments arranged by size: 

                1376     993     587     579     188      37      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     208    2109    2989    3795 
   Size:        208    1901     880     806

  Fragments arranged by size: 

                1901     880     806     208
 
EcoICRI GAG'CTC

Cuts at:      0     588    2145    2214    2685    2787    3795 
   Size:        588    1557      69     471     102    1008

  Fragments arranged by size: 

                1557    1008     588     471     102      69
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     180    1497    1803    2034    2239    2647    2944 
   Size:         85      95    1317     306     231     205     408     297

Cuts at:   2944    3520    3589    3709    3795 
   Size:        576      69     120      86

  Fragments arranged by size: 

                1317     576     408     306     297     231     205     120
                  95      86      85      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1375    1784    2000    2114    2940    3795 
   Size:       1375     409     216     114     826     855

  Fragments arranged by size: 

                1375     855     826     409     216     114
 
EcoRI G'AATT_C

Cuts at:      0     658    3795 
   Size:        658    3137
 
EcoRV GAT'ATC

Cuts at:      0     198    3795 
   Size:        198    3597
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     522    1527    2853    3601    3795 
   Size:        522    1005    1326     748     194

  Fragments arranged by size: 

                1326    1005     748     522     194
 
FspI TGC'GCA

Cuts at:      0    1048    3670    3795 
   Size:       1048    2622     125

  Fragments arranged by size: 

                2622    1048     125
 
HaeII r_GCGC'y

Cuts at:      0    2226    3795 
   Size:       2226    1569
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2233    2265    3795 
   Size:       2233      32    1530

  Fragments arranged by size: 

                2233    1530      32
 
HincII GTy'rAC

Cuts at:      0     360    1289    1398    2916    2976    3795 
   Size:        360     929     109    1518      60     819

  Fragments arranged by size: 

                1518     929     819     360     109      60
 
HindIII A'AGCT_T

Cuts at:      0    1028    3795 
   Size:       1028    2767
 
HpaI GTT'AAC

Cuts at:      0     360    3795 
   Size:        360    3435
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2486    3367    3795 
   Size:       2486     881     428

  Fragments arranged by size: 

                2486     881     428
 
MscI TGG'CCA

Cuts at:      0    1252    3795 
   Size:       1252    2543
 
MslI CAynn'nnrTG

Cuts at:      0     581    1978    3064    3795 
   Size:        581    1397    1086     731

  Fragments arranged by size: 

                1397    1086     731     581
 
MspA1I CmG'CkG

Cuts at:      0     337    1005    1040    1320    1338    1958    3606    3729 
   Size:        337     668      35     280      18     620    1648     123

Cuts at:   3729    3795 
   Size:         66

  Fragments arranged by size: 

                1648     668     620     337     280     123      66      35
                  18
 
NaeI GCC'GGC

Cuts at:      0    2098    3795 
   Size:       2098    1697
 
NcoI C'CATG_G

Cuts at:      0    1113    1772    3795 
   Size:       1113     659    2023

  Fragments arranged by size: 

                2023    1113     659
 
NgoMIV G'CCGG_C

Cuts at:      0    2096    3795 
   Size:       2096    1699
 
NspI r_CATG'y

Cuts at:      0     933    1449    1977    3063    3795 
   Size:        933     516     528    1086     732

  Fragments arranged by size: 

                1086     933     732     528     516
 
PasI CC'CwG_GG

Cuts at:      0     223    3795 
   Size:        223    3572
 
PciI A'CATG_T

Cuts at:      0    1445    3795 
   Size:       1445    2350
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1934    3795 
   Size:       1934    1861
 
PfoI T'CCnGG_A

Cuts at:      0    2216    2624    3566    3795 
   Size:       2216     408     942     229

  Fragments arranged by size: 

                2216     942     408     229
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1579    1611    3795 
   Size:       1579      32    2184

  Fragments arranged by size: 

                2184    1579      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1784    2114    3795 
   Size:       1784     330    1681

  Fragments arranged by size: 

                1784    1681     330
 
PshAI GACnn'nnGTC

Cuts at:      0    2950    3795 
   Size:       2950     845
 
PspOMI G'GGCC_C

Cuts at:      0    1087    1374    3795 
   Size:       1087     287    2421

  Fragments arranged by size: 

                2421    1087     287
 
PspXI vC'TCGA_Gb

Cuts at:      0    1188    3795 
   Size:       1188    2607
 
PstI C_TGCA'G

Cuts at:      0    1228    1643    2490    3116    3795 
   Size:       1228     415     847     626     679

  Fragments arranged by size: 

                1228     847     679     626     415
 
PvuII CAG'CTG

Cuts at:      0     337    1005    1320    1338    3606    3729    3795 
   Size:        337     668     315      18    2268     123      66

  Fragments arranged by size: 

                2268     668     337     315     123      66      18
 
SacI G_AGCT'C

Cuts at:      0     590    2147    2216    2687    2789    3795 
   Size:        590    1557      69     471     102    1006

  Fragments arranged by size: 

                1557    1006     590     471     102      69
 
SacII CC_GC'GG

Cuts at:      0    1959    3795 
   Size:       1959    1836
 
SalI G'TCGA_C

Cuts at:      0    2914    3795 
   Size:       2914     881
 
SanDI GG'GwC_CC

Cuts at:      0    1784    3795 
   Size:       1784    2011
 
SapI GCTCTTCn'nnn_

Cuts at:      0      37    3795 
   Size:         37    3758
 
ScaI AGT'ACT

Cuts at:      0    2452    2755    3795 
   Size:       2452     303    1040

  Fragments arranged by size: 

                2452    1040     303
 
SexAI A'CCwGG_T

Cuts at:      0    2795    3795 
   Size:       2795    1000
 
SfcI C'TryA_G

Cuts at:      0    1224    1568    1639    2486    3112    3795 
   Size:       1224     344      71     847     626     683

  Fragments arranged by size: 

                1224     847     683     626     344      71
 
SmaI CCC'GGG

Cuts at:      0    1993    2409    3795 
   Size:       1993     416    1386

  Fragments arranged by size: 

                1993    1386     416
 
SmlI C'TyrA_G

Cuts at:      0    1188    3795 
   Size:       1188    2607
 
SspI AAT'ATT

Cuts at:      0    2555    3795 
   Size:       2555    1240
 
StuI AGG'CCT

Cuts at:      0    1258    3795 
   Size:       1258    2537
 
StyI C'CwwG_G

Cuts at:      0    1113    1253    1772    2399    2735    2828    3795 
   Size:       1113     140     519     627     336      93     967

  Fragments arranged by size: 

                1113     967     627     519     336     140      93
 
TatI w'GTAC_w

Cuts at:      0    1442    2450    2753    2927    3056    3795 
   Size:       1442    1008     303     174     129     739

  Fragments arranged by size: 

                1442    1008     739     303     174     129
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2380    2412    3023    3055    3795 
   Size:       2380      32     611      32     740

  Fragments arranged by size: 

                2380     740     611      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2673    3795 
   Size:       2673    1122
 
XhoI C'TCGA_G

Cuts at:      0    1188    3795 
   Size:       1188    2607
 
XmaI C'CCGG_G

Cuts at:      0    1991    2407    3795 
   Size:       1991     416    1388

  Fragments arranged by size: 

                1991    1388     416
 
XmnI GAAnn'nnTTC

Cuts at:      0    1594    3795 
   Size:       1594    2201
 
ZraI GAC'GTC

Cuts at:      0    1152    2262    3795 
   Size:       1152    1110    1533

  Fragments arranged by size: 

                1533    1152    1110
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AloI    AlwNI
     ApaI     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PstI    PvuII     SacI    SacII     SalI
    SanDI     SapI     ScaI    SexAI     SfcI     SmaI     SmlI     SspI
     StuI     StyI     TatI     TstI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     AleI     AlfI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BciVI    BfrBI
    BmgBI     BmtI    BpuEI    BsiWI    BspHI   BssHII    BstBI    BstXI
  BstZ17I    CspCI    EcoNI     FseI    FspAI     KasI     KpnI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PsiI     PsrI     PvuI    RsrII     SbfI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SwaI
    TaqII  Tth111I     XbaI
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