Restriction map for pF1KB3253
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       BamHI       BbeI        BbrPI       
BcgI        BclI        BfrI        BglI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRV       FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MslI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII       1490  ACATGT               A^C[GA][CT]GT
AvaI          912  CTCGGG               C^[CT]CG[GA]G
AvaII         833  GGTCC                G^G[AT]CC
AvrII        1522  CCTAGG               C^CTAGG
BanI          638  GGTGCC               G^G[CT][GA]CC
BbvCI         391  GCTGAGG              CCTCAGC(-5/-2)
BciVI         711  GGATAC               GTATCC(6/5)
BglII         315  AGATCT               A^GATCT
BlnI         1522  CCTAGG               C^CTAGG
BpmI          643  CTGGAG               CTGGAG(16/14)
BsmAI        1177  GTCTC                GTCTC(1/5)
BsmFI         452  GGGAC                GGGAC(10/14)
BsmI          729  GAATGC               GAATGC(1/-1)
BsoBI         912  CTCGGG               C^[CT]CG[GA]G
BspHI         827  TCATGA               T^CATGA
BspLU11I     1490  ACATGT               A^CATGT
BspMI        1818  ACCTGC               ACCTGC(4/8)
BsrDI         733  GCAATG               GCAATG(2/0)
BstAPI       1716  GCAGACTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1745  TTCGAA               TT^CGAA
BstEII       1162  GGTGACC              G^GT[ACGT]ACC
BstPI        1162  GGTGACC              G^GT[ACGT]ACC
Cfr13I        833  GGTCC                G^G[ACGT]CC
DraI         1708  TTTAAA               TTT^AAA
EcoO65I      1162  GGTGACC              G^GT[ACGT]ACC
EcoRI        1246  GAATTC               G^AATTC
EcoT22I       323  ATGCAT               ATGCA^T
MaeII         704  ACGT                 A^CGT
MspA1I        749  CAGCTG               C[AC]G^C[GT]G
NsiI          323  ATGCAT               ATGCA^T
NspI         1490  ACATGT               [GA]CATG^[CT]
NspV         1745  TTCGAA               TT^CGAA
PflMI         616  CCACTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI          18  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          950  CTGCAG               CTGCA^G
PvuII         749  CAGCTG               CAG^CTG
RcaI          827  TCATGA               T^CATGA
Sau96I        833  GGTCC                G^G[ACGT]CC
ScaI         1400  AGTACT               AGT^ACT
SfuI         1745  TTCGAA               TT^CGAA
SmlI         1342  CTCAAG               C^T[CT][GA]AG
Tsp45I       1163  GTGAC                ^GT[GC]AC
Van91I        616  CCACTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          418  CCAAACTCACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccIII         11  TCCGGA               T^CCGGA
              539  TCCGGA              
AcsI         1132  AAATTT               [GA]^AATT[CT]
             1246  GAATTC              
AlwI         1272  GATCC                GGATC(4/5)
             1115  GGATC               
AlwNI        1717  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1814  CAGAACCTG           
ApoI         1132  AAATTT               [GA]^AATT[CT]
             1246  GAATTC              
AspHI         909  GAGCTC               G[AT]GC[AT]^C
             1339  GAGCTC              
BanII         909  GAGCTC               G[GA]GC[CT]^C
             1339  GAGCTC              
BfaI         1523  CTAG                 C^TAG
             1597  CTAG                
BmyI          909  GAGCTC               G[AGT]GC[ACT]^C
             1339  GAGCTC              
BseAI          11  TCCGGA               T^CCGGA
              539  TCCGGA              
BsiHKAI       909  GAGCTC               G[AT]GC[AT]^C
             1339  GAGCTC              
Bsp1286I      909  GAGCTC               G[AGT]GC[ACT]^C
             1339  GAGCTC              
BspEI          11  TCCGGA               T^CCGGA
              539  TCCGGA              
BstXI         635  CCAGGTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1152  CCAGTTGGATGG        
Cac8I         371  GCTGGC               GC[ACGT]^[ACGT]GC
              505  GCAAGC              
EcoNI        1067  CCTACTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1644  CCTCACCAAGG         
MaeI         1523  CTAG                 C^TAG
             1597  CTAG                
MaeIII       1163  GTGAC                ^GT[ACGT]AC
             1659  GTTAC               
MroI           11  TCCGGA               T^CCGGA
              539  TCCGGA              
NlaIV         638  GGTGCC               GG[ACGT]^[ACGT]CC
             1449  GGAGCC              
PleI          118  GACTC                GAGTC(4/5)
             1719  GACTC               
SacI          909  GAGCTC               GAGCT^C
             1339  GAGCTC              
SapI         1336  GAAGAGC              GCTCTTC(1/4)
             1741  GCTCTTC             
SspI          787  AATATT               AAT^ATT
              817  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AfaI        AluI        Asp700I     BalI        BbsI        
BbvI        BcnI        BpuAI       BsaJI       BsaWI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       BstYI       DdeI        DpnII       EaeI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
RsaI        Sau3AI      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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