Restriction map for pF1KB3223
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AgeI        Alw44I      AlwNI       AocI        ApaI        
ApaLI       AscI        AspHI       AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BlnI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsoBI       
BspDI       BspEI       BspMI       BsrBI       BsrFI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco52I      Eco81I      EcoO65I     EcoT14I     
EcoT22I     FseI        FspI        HapII       HgaI        
Hin1I       HindIII     HpaI        HpaII       KasI        
KspI        MamI        MroI        MspA1I      MspI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I       1114  GGTACC               G^GTACC
AflII        2008  CTTAAG               C^TTAAG
AhdI         1681  GACATGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       519  AGCGCT               AGC^GCT
AseI         1849  ATTAAT               AT^TAAT
AsnI         1849  ATTAAT               AT^TAAT
Asp700I      1651  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1114  GGTACC               G^GTACC
AspEI        1681  GACATGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1486  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1126  TGGCCA               TGG^CCA
BclI          249  TGATCA               T^GATCA
BfrI         2008  CTTAAG               C^TTAAG
BglI         2038  GCCGTATCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         408  AGATCT               A^GATCT
BlpI           48  GCTGAGC              GC^T[ACGT]AGC
BpmI         1136  CTGGAG               CTGGAG(16/14)
Bpu1102I       48  GCTGAGC              GC^T[ACGT]AGC
BsaI          498  GAGACC               GGTCTC(1/5)
Bsp1407I      356  TGTACA               T^GTACA
BspLU11I      776  ACATGT               A^CATGT
BsrDI        1231  GCAATG               GCAATG(2/0)
BsrGI         356  TGTACA               T^GTACA
BstXI        2099  CCAACCCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         408  AGATCT               [GA]^GATC[CT]
CelII          48  GCTGAGC              GC^T[ACGT]AGC
CfoI          520  GCGC                 GCG^C
DsaI         1940  CCGTGG               C^C[GA][CT]GG
Eam1105I     1681  GACATGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1896  GAAGAG               CTCTTC(1/4)
Eco47III      519  AGCGCT               AGC^GCT
EcoNI        1105  CCTCTCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         752  GAATTC               G^AATTC
EcoRV        1714  GATATC               GAT^ATC
FauI         1842  GCGGG                CCCGC(4/6)
FbaI          249  TGATCA               T^GATCA
HaeII         519  AGCGCT               [GA]GCGC^[CT]
HhaI          520  GCGC                 GCG^C
HinP1I        520  GCGC                 G^CGC
HincII        191  GTCAAC               GT[CT]^[GA]AC
HindII        191  GTCAAC               GT[CT]^[GA]AC
KpnI         1114  GGTACC               GGTAC^C
Ksp632I      1896  GAAGAG               CTCTTC(1/4)
MaeII         920  ACGT                 A^CGT
MfeI         1400  CAATTG               C^AATTG
MflI          408  AGATCT               [GA]^GATC[CT]
MluI         1836  ACGCGT               A^CGCGT
MluNI        1126  TGGCCA               TGG^CCA
MscI         1126  TGGCCA               TGG^CCA
MunI         1400  CAATTG               C^AATTG
NdeI         2113  CATATG               CA^TATG
NheI          664  GCTAGC               G^CTAGC
NspI          776  ACATGT               [GA]CATG^[CT]
PflFI        1486  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        1849  ATTAAT               AT^TAAT
PstI          515  CTGCAG               CTGCA^G
ScaI         1747  AGTACT               AGT^ACT
SspBI         356  TGTACA               T^GTACA
Tth111I      1486  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          735  CCAGCCTGGTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         408  AGATCT               [GA]^GATC[CT]
XmnI         1651  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII         895  CGCG                 CG^CG
             1837  CGCG                
AcsI          597  AAATTC               [GA]^AATT[CT]
              752  GAATTC              
AflIII        776  ACATGT               A^C[GA][CT]GT
             1836  ACGCGT              
ApoI          597  AAATTC               [GA]^AATT[CT]
              752  GAATTC              
BanI          555  GGTGCC               G^G[CT][GA]CC
             1114  GGTACC              
BanII         275  GAGCCC               G[GA]GC[CT]^C
              423  GAGCCC              
BbsI         1483  GAAGAC               GAAGAC(2/6)
              869  GTCTTC              
BmyI          275  GAGCCC               G[AGT]GC[ACT]^C
              423  GAGCCC              
BpuAI        1483  GAAGAC               GAAGAC(2/6)
              869  GTCTTC              
Bsp1286I      275  GAGCCC               G[AGT]GC[ACT]^C
              423  GAGCCC              
BspHI         305  TCATGA               T^CATGA
             1659  TCATGA              
BssSI         864  CACGAG               CACGAG(-5/-1)
             1213  CACGAG              
BstUI         895  CGCG                 CG^CG
             1837  CGCG                
MvnI          895  CGCG                 CG^CG
             1837  CGCG                
RcaI          305  TCATGA               T^CATGA
             1659  TCATGA              
SfcI           29  CTATAG               C^T[GA][CT]AG
              515  CTGCAG              
SmlI          822  CTTGAG               C^T[CT][GA]AG
             2008  CTTAAG              
Tsp45I       1355  GTCAC                ^GT[GC]AC
             1531  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BfaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        
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