Restriction map for pF1KB3223
 (Linear) (Six-base) MAPSORT of: KIBB3223.seq  Check: 9883  from: 1  to: 2118



KIBB3223.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

Acc65I G'GTAC_C

Cuts at:      0    1114    2118 
   Size:       1114    1004
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     428    2118 
   Size:         51     377    1690

  Fragments arranged by size: 

                1690     377      51
 
AfeI AGC'GCT

Cuts at:      0     521    2118 
   Size:        521    1597
 
AflII C'TTAA_G

Cuts at:      0    2008    2118 
   Size:       2008     110
 
AflIII A'CryG_T

Cuts at:      0     776    1836    2118 
   Size:        776    1060     282

  Fragments arranged by size: 

                1060     776     282
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1686    2118 
   Size:       1686     432
 
ApoI r'AATT_y

Cuts at:      0     597     752    2118 
   Size:        597     155    1366

  Fragments arranged by size: 

                1366     597     155
 
AseI AT'TA_AT

Cuts at:      0    1850    2118 
   Size:       1850     268
 
BanI G'GyrC_C

Cuts at:      0     555    1114    2118 
   Size:        555     559    1004

  Fragments arranged by size: 

                1004     559     555
 
BanII G_rGCy'C

Cuts at:      0     279     427    2118 
   Size:        279     148    1691

  Fragments arranged by size: 

                1691     279     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     862    1490    2118 
   Size:        862     628     628

  Fragments arranged by size: 

                 862     628     628
 
BclI T'GATC_A

Cuts at:      0     249    2118 
   Size:        249    1869
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2044    2118 
   Size:       2044      74
 
BglII A'GATC_T

Cuts at:      0     408    2118 
   Size:        408    1710
 
BlpI GC'TnA_GC

Cuts at:      0      49    2118 
   Size:         49    2069
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     130    2118 
   Size:        130    1988
 
BmtI G_CTAG'C

Cuts at:      0     668    2118 
   Size:        668    1450
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     360     848    2118 
   Size:        360     488    1270

  Fragments arranged by size: 

                1270     488     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1157    2118 
   Size:       1157     961
 
Bpu10I CC'TnA_GC

Cuts at:      0     641    2118 
   Size:        641    1477
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     843    2118 
   Size:        843    1275
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     492    2118 
   Size:        492    1626
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     176     510     929    2118 
   Size:        176     334     419    1189

  Fragments arranged by size: 

                1189     419     334     176
 
BseYI C'CCAG_C

Cuts at:      0       8     426    2118 
   Size:          8     418    1692

  Fragments arranged by size: 

                1692     418       8
 
BsmI GAATG_Cn'

Cuts at:      0     962     987    1858    2118 
   Size:        962      25     871     260

  Fragments arranged by size: 

                 962     871     260      25
 
Bsp1286I G_dGCh'C

Cuts at:      0     279     427    2118 
   Size:        279     148    1691

  Fragments arranged by size: 

                1691     279     148
 
BspHI T'CATG_A

Cuts at:      0     305    1659    2118 
   Size:        305    1354     459

  Fragments arranged by size: 

                1354     459     305
 
BsrDI GCAATG_nn'

Cuts at:      0    1238    2118 
   Size:       1238     880
 
BsrGI T'GTAC_A

Cuts at:      0     356    2118 
   Size:        356    1762
 
BssSI C'ACGA_G

Cuts at:      0     864    1213    2118 
   Size:        864     349     905

  Fragments arranged by size: 

                 905     864     349
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2106    2118 
   Size:       2106      12
 
BstYI r'GATC_y

Cuts at:      0     408    2118 
   Size:        408    1710
 
BtgI C'CryG_G

Cuts at:      0    1940    2118 
   Size:       1940     178
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      88     877    2118 
   Size:         88     789    1241

  Fragments arranged by size: 

                1241     789      88
 
BtsI GCAGTG_nn'

Cuts at:      0    2062    2118 
   Size:       2062      56
 
EaeI y'GGCC_r

Cuts at:      0      23    1126    1872    2118 
   Size:         23    1103     746     246

  Fragments arranged by size: 

                1103     746     246      23
 
EarI CTCTTCn'nnn_

Cuts at:      0    1891    2118 
   Size:       1891     227
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1821    2118 
   Size:       1821     297
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     428    1157    2118 
   Size:         51     377     729     961

  Fragments arranged by size: 

                 961     729     377      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1109    2118 
   Size:       1109    1009
 
EcoO109I rG'GnC_Cy

Cuts at:      0     689     761    1330    2118 
   Size:        689      72     569     788

  Fragments arranged by size: 

                 788     689     569      72
 
EcoRI G'AATT_C

Cuts at:      0     752    2118 
   Size:        752    1366
 
EcoRV GAT'ATC

Cuts at:      0    1716    2118 
   Size:       1716     402
 
HaeII r_GCGC'y

Cuts at:      0     523    2118 
   Size:        523    1595
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     393     425    1219    1251    1672    1684    1687    1704 
   Size:        393      32     794      32     421      12       3      17

Cuts at:   1704    1716    1719    2118 
   Size:         12       3     399

  Fragments arranged by size: 

                 794     421     399     393      32      32      17      12
                  12       3       3
 
HincII GTy'rAC

Cuts at:      0     193    2118 
   Size:        193    1925
 
KpnI G_GTAC'C

Cuts at:      0    1118    2118 
   Size:       1118    1000
 
MfeI C'AATT_G

Cuts at:      0    1400    2118 
   Size:       1400     718
 
MluI A'CGCG_T

Cuts at:      0    1836    2118 
   Size:       1836     282
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1071    1449    2118 
   Size:       1071     378     669

  Fragments arranged by size: 

                1071     669     378
 
MscI TGG'CCA

Cuts at:      0    1128    2118 
   Size:       1128     990
 
MslI CAynn'nnrTG

Cuts at:      0     245     257     812    2118 
   Size:        245      12     555    1306

  Fragments arranged by size: 

                1306     555     245      12
 
NdeI CA'TA_TG

Cuts at:      0    2114    2118 
   Size:       2114       4
 
NheI G'CTAG_C

Cuts at:      0     664    2118 
   Size:        664    1454
 
NspI r_CATG'y

Cuts at:      0     780    2118 
   Size:        780    1338
 
PciI A'CATG_T

Cuts at:      0     776    2118 
   Size:        776    1342
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1124    1135    1326    2118 
   Size:       1124      11     191     792

  Fragments arranged by size: 

                1124     792     191      11
 
PfoI T'CCnGG_A

Cuts at:      0     490    2118 
   Size:        490    1628
 
PpuMI rG'GwC_Cy

Cuts at:      0     689     761    1330    2118 
   Size:        689      72     569     788

  Fragments arranged by size: 

                 788     689     569      72
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     894     926    1596    1628    1731    1763    2118 
   Size:        894      32     670      32     103      32     355

  Fragments arranged by size: 

                 894     670     355     103      32      32      32
 
PstI C_TGCA'G

Cuts at:      0     519    2118 
   Size:        519    1599
 
SanDI GG'GwC_CC

Cuts at:      0     689    2118 
   Size:        689    1429
 
ScaI AGT'ACT

Cuts at:      0    1749    2118 
   Size:       1749     369
 
SfcI C'TryA_G

Cuts at:      0      29     515    2118 
   Size:         29     486    1603

  Fragments arranged by size: 

                1603     486      29
 
SmlI C'TyrA_G

Cuts at:      0     822    2008    2118 
   Size:        822    1186     110

  Fragments arranged by size: 

                1186     822     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      52    2118 
   Size:         52    2066
 
TatI w'GTAC_w

Cuts at:      0     356    1525    1747    2118 
   Size:        356    1169     222     371

  Fragments arranged by size: 

                1169     371     356     222
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     855     887    2118 
   Size:        855      32    1231

  Fragments arranged by size: 

                1231     855      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1489    2118 
   Size:       1489     629
 
XbaI T'CTAG_A

Cuts at:      0     217    1729    1959    2118 
   Size:        217    1512     230     159

  Fragments arranged by size: 

                1512     230     217     159
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     742    2118 
   Size:        742    1376
 
XmnI GAAnn'nnTTC

Cuts at:      0    1655    2118 
   Size:       1655     463
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI    AflII   AflIII     AhdI     ApoI     AseI
     BanI    BanII     BbsI     BclI     BglI    BglII     BlpI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BseYI
     BsmI Bsp1286I    BspHI    BsrDI    BsrGI    BssSI    BstXI    BstYI
     BtgI    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NdeI     NheI     NspI     PciI
    PflMI     PfoI    PpuMI     PsrI     PstI    SanDI     ScaI     SfcI
     SmlI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AgeI     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI Bme1580I    BmgBI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI     EagI  EcoICRI     FalI     FseI     FspI    FspAI  HindIII
     HpaI     KasI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     XhoI     XmaI     ZraI
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