Restriction map for pF1KB3106
 (Linear) (Six-base) MAPSORT of: KIBB3106.seq  Check: 7227  from: 1  to: 2511



KIBB3106.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AccI GT'mk_AC

Cuts at:      0     833    2511 
   Size:        833    1678
 
Acc65I G'GTAC_C

Cuts at:      0    2045    2511 
   Size:       2045     466
 
AclI AA'CG_TT

Cuts at:      0     869    2153    2511 
   Size:        869    1284     358

  Fragments arranged by size: 

                1284     869     358
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     662    1972    2511 
   Size:        454     208    1310     539

  Fragments arranged by size: 

                1310     539     454     208
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1913    2511 
   Size:       1913     598
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1633    1995    2493    2511 
   Size:       1633     362     498      18

  Fragments arranged by size: 

                1633     498     362      18
 
ApaI G_GGCC'C

Cuts at:      0     213    2511 
   Size:        213    2298
 
ApaLI G'TGCA_C

Cuts at:      0    1534    2511 
   Size:       1534     977
 
ApoI r'AATT_y

Cuts at:      0    2107    2511 
   Size:       2107     404
 
AvaI C'yCGr_G

Cuts at:      0     502     952    2511 
   Size:        502     450    1559

  Fragments arranged by size: 

                1559     502     450
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2037    2070    2511 
   Size:       2037      33     441

  Fragments arranged by size: 

                2037     441      33
 
BamHI G'GATC_C

Cuts at:      0     580    1317    1652    2511 
   Size:        580     737     335     859

  Fragments arranged by size: 

                 859     737     580     335
 
BanI G'GyrC_C

Cuts at:      0    1342    1742    2045    2158    2472    2511 
   Size:       1342     400     303     113     314      39

  Fragments arranged by size: 

                1342     400     314     303     113      39
 
BanII G_rGCy'C

Cuts at:      0     213     254    2511 
   Size:        213      41    2257

  Fragments arranged by size: 

                2257     213      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1785    2352    2470    2511 
   Size:       1785     567     118      41

  Fragments arranged by size: 

                1785     567     118      41
 
BbvCI CC'TCA_GC

Cuts at:      0    1117    2342    2511 
   Size:       1117    1225     169

  Fragments arranged by size: 

                1225    1117     169
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1741    1775    2511 
   Size:       1741      34     736

  Fragments arranged by size: 

                1741     736      34
 
BfrBI ATG'CAT

Cuts at:      0    1900    2511 
   Size:       1900     611
 
BlpI GC'TnA_GC

Cuts at:      0     664    2511 
   Size:        664    1847
 
Bme1580I G_kGCm'C

Cuts at:      0     213    1203    1538    1841    2309    2477    2511 
   Size:        213     990     335     303     468     168      34

  Fragments arranged by size: 

                 990     468     335     303     213     168      34
 
BmgBI CAC'GTC

Cuts at:      0    2468    2511 
   Size:       2468      43
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      93    1476    1929    2511 
   Size:         93    1383     453     582

  Fragments arranged by size: 

                1383     582     453      93
 
BmtI G_CTAG'C

Cuts at:      0    2300    2511 
   Size:       2300     211
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     189    1371    2511 
   Size:        189    1182    1140

  Fragments arranged by size: 

                1182    1140     189
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     979    1087    1150    1708    1759    2511 
   Size:         76     903     108      63     558      51     752

  Fragments arranged by size: 

                 903     752     558     108      76      63      51
 
Bpu10I CC'TnA_GC

Cuts at:      0    1117    2342    2511 
   Size:       1117    1225     169

  Fragments arranged by size: 

                1225    1117     169
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     776    2511 
   Size:        238     538    1735

  Fragments arranged by size: 

                1735     538     238
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2326    2511 
   Size:       2326     185
 
BsaBI GATnn'nnATC

Cuts at:      0    2428    2511 
   Size:       2428      83
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     265     295    2511 
   Size:        265      30    2216

  Fragments arranged by size: 

                2216     265      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     286     661    1111    1156    1252    1746    2044    2359 
   Size:        286     375     450      45      96     494     298     315

Cuts at:   2359    2511 
   Size:        152

  Fragments arranged by size: 

                 494     450     375     315     298     286     152      96
                  45
 
BseYI C'CCAG_C

Cuts at:      0     190     379     431    1747    1845    2511 
   Size:        190     189      52    1316      98     666

  Fragments arranged by size: 

                1316     666     190     189      98      52
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1317    2511 
   Size:       1317    1194
 
BsiEI CG_ry'CG

Cuts at:      0    1257    2436    2511 
   Size:       1257    1179      75

  Fragments arranged by size: 

                1257    1179      75
 
BsiHKAI G_wGCw'C

Cuts at:      0     965    1538    2002    2181    2351    2511 
   Size:        965     573     464     179     170     160

  Fragments arranged by size: 

                 965     573     464     179     170     160
 
BsmI GAATG_Cn'

Cuts at:      0     516    1816    1900    2511 
   Size:        516    1300      84     611

  Fragments arranged by size: 

                1300     611     516      84
 
Bsp1286I G_dGCh'C

Cuts at:      0     213     254     965    1203    1538    1841    2002    2181 
   Size:        213      41     711     238     335     303     161     179

Cuts at:   2181    2309    2351    2477    2511 
   Size:        128      42     126      34

  Fragments arranged by size: 

                 711     335     303     238     213     179     161     128
                 126      42      41      34
 
BspHI T'CATG_A

Cuts at:      0    1292    2129    2511 
   Size:       1292     837     382

  Fragments arranged by size: 

                1292     837     382
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     977    2511 
   Size:        977    1534
 
BsrBI CCG'CTC

Cuts at:      0      60    1035    1101    1152    1623    2214    2511 
   Size:         60     975      66      51     471     591     297

  Fragments arranged by size: 

                 975     591     471     297      66      60      51
 
BsrDI GCAATG_nn'

Cuts at:      0    2189    2511 
   Size:       2189     322
 
BsrFI r'CCGG_y

Cuts at:      0    1016    2511 
   Size:       1016    1495
 
BssSI C'ACGA_G

Cuts at:      0    1093    2511 
   Size:       1093    1418
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     969    2511 
   Size:        969    1542
 
BstBI TT'CG_AA

Cuts at:      0    1211    1467    2511 
   Size:       1211     256    1044

  Fragments arranged by size: 

                1211    1044     256
 
BstYI r'GATC_y

Cuts at:      0      87     580    1317    1553    1652    2511 
   Size:         87     493     737     236      99     859

  Fragments arranged by size: 

                 859     737     493     236      99      87
 
Bsu36I CC'TnA_GG

Cuts at:      0     332    2511 
   Size:        332    2179
 
BtgI C'CryG_G

Cuts at:      0     725    1398    1660    2511 
   Size:        725     673     262     851

  Fragments arranged by size: 

                 851     725     673     262
 
BtsI GCAGTG_nn'

Cuts at:      0     767    2482    2511 
   Size:        767    1715      29

  Fragments arranged by size: 

                1715     767      29
 
DraIII CAC_nnn'GTG

Cuts at:      0    1484    2042    2511 
   Size:       1484     558     469

  Fragments arranged by size: 

                1484     558     469
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1045    2511 
   Size:       1045    1466
 
EaeI y'GGCC_r

Cuts at:      0    1274    2291    2412    2504    2511 
   Size:       1274    1017     121      92       7

  Fragments arranged by size: 

                1274    1017     121      92       7
 
EarI CTCTTCn'nnn_

Cuts at:      0     296     638     725    1060    2511 
   Size:        296     342      87     335    1451

  Fragments arranged by size: 

                1451     342     335     296      87
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1170    1418    2511 
   Size:       1170     248    1093

  Fragments arranged by size: 

                1170    1093     248
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     454     662     979    1087    1150    1708    1759 
   Size:         76     378     208     317     108      63     558      51

Cuts at:   1759    1972    2511 
   Size:        213     539

  Fragments arranged by size: 

                 558     539     378     317     213     208     108      76
                  63      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1120    1431    1675    2511 
   Size:       1120     311     244     836

  Fragments arranged by size: 

                1120     836     311     244
 
EcoO109I rG'GnC_Cy

Cuts at:      0     498     933    1083    1125    1410    2322    2511 
   Size:        498     435     150      42     285     912     189

  Fragments arranged by size: 

                 912     498     435     285     189     150      42
 
EcoRV GAT'ATC

Cuts at:      0    2127    2511 
   Size:       2127     384
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1320    1809    1989    2511 
   Size:       1320     489     180     522

  Fragments arranged by size: 

                1320     522     489     180
 
HaeII r_GCGC'y

Cuts at:      0     123    1730    2511 
   Size:        123    1607     781

  Fragments arranged by size: 

                1607     781     123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     485     517    1397    1429    2511 
   Size:        485      32     880      32    1082

  Fragments arranged by size: 

                1082     880     485      32      32
 
HincII GTy'rAC

Cuts at:      0     234     624    1050    2511 
   Size:        234     390     426    1461

  Fragments arranged by size: 

                1461     426     390     234
 
HindIII A'AGCT_T

Cuts at:      0     463    2511 
   Size:        463    2048
 
KpnI G_GTAC'C

Cuts at:      0    2049    2511 
   Size:       2049     462
 
MfeI C'AATT_G

Cuts at:      0     533    2511 
   Size:        533    1978
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     263    1277    2035    2174    2511 
   Size:        263    1014     758     139     337

  Fragments arranged by size: 

                1014     758     337     263     139
 
MscI TGG'CCA

Cuts at:      0    1276    2506    2511 
   Size:       1276    1230       5

  Fragments arranged by size: 

                1276    1230       5
 
MslI CAynn'nnrTG

Cuts at:      0     808    1895    2287    2511 
   Size:        808    1087     392     224

  Fragments arranged by size: 

                1087     808     392     224
 
MspA1I CmG'CkG

Cuts at:      0     268     431     906     975    2511 
   Size:        268     163     475      69    1536

  Fragments arranged by size: 

                1536     475     268     163      69
 
NcoI C'CATG_G

Cuts at:      0    1398    1660    2511 
   Size:       1398     262     851

  Fragments arranged by size: 

                1398     851     262
 
NheI G'CTAG_C

Cuts at:      0    2296    2511 
   Size:       2296     215
 
NsiI A_TGCA'T

Cuts at:      0    1902    2511 
   Size:       1902     609
 
NspI r_CATG'y

Cuts at:      0    2169    2511 
   Size:       2169     342
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     916    1065    2082    2511 
   Size:        916     149    1017     429

  Fragments arranged by size: 

                1017     916     429     149
 
PfoI T'CCnGG_A

Cuts at:      0    1085    1127    2511 
   Size:       1085      42    1384

  Fragments arranged by size: 

                1384    1085      42
 
PpuMI rG'GwC_Cy

Cuts at:      0    1083    1125    1410    2322    2511 
   Size:       1083      42     285     912     189

  Fragments arranged by size: 

                1083     912     285     189      42
 
PshAI GACnn'nnGTC

Cuts at:      0    1081    2511 
   Size:       1081    1430
 
PspOMI G'GGCC_C

Cuts at:      0     209    2511 
   Size:        209    2302
 
PstI C_TGCA'G

Cuts at:      0     974    1174    1223    1736    2489    2511 
   Size:        974     200      49     513     753      22

  Fragments arranged by size: 

                 974     753     513     200      49      22
 
PvuII CAG'CTG

Cuts at:      0     431     906     975    2511 
   Size:        431     475      69    1536

  Fragments arranged by size: 

                1536     475     431      69
 
SbfI CC_TGCA'GG

Cuts at:      0    1223    2511 
   Size:       1223    1288
 
ScaI AGT'ACT

Cuts at:      0    1231    1303    2511 
   Size:       1231      72    1208

  Fragments arranged by size: 

                1231    1208      72
 
SfcI C'TryA_G

Cuts at:      0     837     843     892     970    1024    1170    1219    1732 
   Size:        837       6      49      78      54     146      49     513

Cuts at:   1732    2485    2511 
   Size:        753      26

  Fragments arranged by size: 

                 837     753     513     146      78      54      49      49
                  26       6
 
SmlI C'TyrA_G

Cuts at:      0     253     791    2511 
   Size:        253     538    1720

  Fragments arranged by size: 

                1720     538     253
 
SspI AAT'ATT

Cuts at:      0    1473    2511 
   Size:       1473    1038
 
StyI C'CwwG_G

Cuts at:      0     134     346     743     929    1398    1492    1660    2336 
   Size:        134     212     397     186     469      94     168     676

Cuts at:   2336    2511 
   Size:        175

  Fragments arranged by size: 

                 676     469     397     212     186     175     168     134
                  94
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1093    1495    1521    1673    1899    2218    2460    2511 
   Size:       1093     402      26     152     226     319     242      51

  Fragments arranged by size: 

                1093     402     319     242     226     152      51      26
            
 
TatI w'GTAC_w

Cuts at:      0    1229    1241    1301    2511 
   Size:       1229      12      60    1210

  Fragments arranged by size: 

                1229    1210      60      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     414    2046    2511 
   Size:        414    1632     465

  Fragments arranged by size: 

                1632     465     414
 
XmnI GAAnn'nnTTC

Cuts at:      0     100    2224    2511 
   Size:        100    2124     287

  Fragments arranged by size: 

                2124     287     100
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     KpnI
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NheI     NsiI
     NspI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SbfI     ScaI     SfcI     SmlI     SspI     StyI    TaqII     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AloI     AscI     AseI    AsiSI    AvrII     BbeI    BciVI     BclI
     BglI    BglII    BsaAI    BsaHI    BsaWI    BsiWI    BsmBI    BspEI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     EagI  EcoICRI    EcoRI     FseI     FspI    FspAI     HpaI
     KasI     MluI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     PacI     PasI     PciI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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