Restriction map for pF1KB3093
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      AlwI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AvrII       
BamHI       BbrPI       BbsI        BbvCI       BfrI        
BlnI        BpuAI       BsaI        BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DraIII      Eco47III    
EcoRI       EcoRV       EcoT22I     FseI        HindIII     
HpaI        KpnI        KspI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tsp509I     Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        1210  GACGTC               GACGT^C
AhdI          987  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         987  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1127  TGCGCA               TGC^GCA
BcgI         1914  CGAGGGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          74  GTATCC               GTATCC(6/5)
BfaI         1388  CTAG                 C^TAG
BglII        1736  AGATCT               A^GATCT
BlpI          702  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      702  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1621  CACGTA               [CT]AC^GT[GA]
BsaBI        2058  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1850  CGGCCG               CG[GA][CT]^CG
BsmBI        1429  CGTCTC               CGTCTC(1/5)
BssSI         943  CTCGTG               CACGAG(-5/-1)
BstEII        474  GGTGACC              G^GT[ACGT]ACC
BstPI         474  GGTGACC              G^GT[ACGT]ACC
BstYI        1736  AGATCT               [GA]^GATC[CT]
CelII         702  GCTCAGC              GC^T[ACGT]AGC
DrdI         1210  GACGTCAACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1850  CGGCCG               C^GGCCG
Eam1105I      987  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         2014  CTCTTC               CTCTTC(1/4)
EclXI        1850  CGGCCG               C^GGCCG
Eco52I       1850  CGGCCG               C^GGCCG
EcoNI         762  CCTGTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       474  GGTGACC              G^GT[ACGT]ACC
FspI         1127  TGCGCA               TGC^GCA
HincII       1213  GTCAAC               GT[CT]^[GA]AC
HindII       1213  GTCAAC               GT[CT]^[GA]AC
Ksp632I      2014  CTCTTC               CTCTTC(1/4)
MaeI         1388  CTAG                 C^TAG
MamI         2058  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1736  AGATCT               [GA]^GATC[CT]
SfiI         1404  GGCCGAGATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1712  CCCGGG               CCC^GGG
SphI          698  GCATGC               GCATG^C
StuI          140  AGGCCT               AGG^CCT
XcmI         1721  CCATGAACCGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1736  AGATCT               [GA]^GATC[CT]
XmaCI        1712  CCCGGG               C^CCGGG
XmaI         1712  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AocI         1399  CCTGAGG              CC^T[ACGT]AGG
             2196  CCTCAGG             
ApaI          530  GGGCCC               GGGCC^C
              931  GGGCCC              
BclI          911  TGATCA               T^GATCA
             1511  TGATCA              
BsaWI        1690  ACCGGA               [AT]^CCGG[AT]
             1979  ACCGGA              
BseRI         130  GAGGAG               GAGGAG(10/8)
             1669  GAGGAG              
BsmAI        2165  GAGAC                GTCTC(1/5)
             1430  GTCTC               
BstAPI        750  GCACAACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              948  GCAGATCATGC         
Bsu36I       1399  CCTGAGG              CC^T[ACGT]AGG
             2196  CCTCAGG             
DsaI         1232  CCACGG               C^C[GA][CT]GG
             1994  CCGTGG              
Eco81I       1399  CCTGAGG              CC^T[ACGT]AGG
             2196  CCTCAGG             
EcoT14I       204  CCAAGG               C^C[AT][AT]GG
              479  CCAAGG              
FbaI          911  TGATCA               T^GATCA
             1511  TGATCA              
PleI           67  GACTC                GAGTC(4/5)
              718  GAGTC               
PpuMI         461  AGGACCC              [GA]G^G[AT]CC[CT]
             1715  GGGACCC             
SacI          436  GAGCTC               GAGCT^C
             1999  GAGCTC              
StyI          204  CCAAGG               C^C[AT][AT]GG
              479  CCAAGG              
TaqI          508  TCGA                 T^CGA
             2177  TCGA                
TthHB8I       508  TCGA                 T^CGA
             2177  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbvI        BcnI        
BglI        BmyI        BpmI        BsaHI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmlI        TfiI        TseI        
Tsp45I      TspRI       Van91I      
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