Restriction map for pF1KB3043
 (Linear) (Six-base) MAPSORT of: KIBB3043.seq  Check: 308  from: 1  to: 3456



KIBB3043.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AccI GT'mk_AC

Cuts at:      0    1796    2654    2774    3456 
   Size:       1796     858     120     682

  Fragments arranged by size: 

                1796     858     682     120
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     599     982    1047    2334    3456 
   Size:        106     493     383      65    1287    1122

  Fragments arranged by size: 

                1287    1122     493     383     106      65
 
AfeI AGC'GCT

Cuts at:      0    1068    2512    3007    3456 
   Size:       1068    1444     495     449

  Fragments arranged by size: 

                1444    1068     495     449
 
AflIII A'CryG_T

Cuts at:      0     257     347     932    3456 
   Size:        257      90     585    2524

  Fragments arranged by size: 

                2524     585     257      90
 
AhdI GACnn_n'nnGTC

Cuts at:      0      38    3456 
   Size:         38    3418
 
AleI CACnn'nnGTG

Cuts at:      0     352    2560    3456 
   Size:        352    2208     896

  Fragments arranged by size: 

                2208     896     352
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1532    3456 
   Size:       1532    1924
 
AlwNI CAG_nnn'CTG

Cuts at:      0     306    1681    1758    1958    2413    3456 
   Size:        306    1375      77     200     455    1043

  Fragments arranged by size: 

                1375    1043     455     306     200      77
 
ApaI G_GGCC'C

Cuts at:      0    1437    1626    2390    3456 
   Size:       1437     189     764    1066

  Fragments arranged by size: 

                1437    1066     764     189
 
ApaLI G'TGCA_C

Cuts at:      0     325    3456 
   Size:        325    3131
 
ApoI r'AATT_y

Cuts at:      0     583    2740    3124    3456 
   Size:        583    2157     384     332

  Fragments arranged by size: 

                2157     583     384     332
 
AvaI C'yCGr_G

Cuts at:      0    1457    2390    2982    3438    3456 
   Size:       1457     933     592     456      18

  Fragments arranged by size: 

                1457     933     592     456      18
 
BamHI G'GATC_C

Cuts at:      0     127     379    3456 
   Size:        127     252    3077

  Fragments arranged by size: 

                3077     252     127
 
BanI G'GyrC_C

Cuts at:      0    1266    1348    1360    2528    2916    3456 
   Size:       1266      82      12    1168     388     540

  Fragments arranged by size: 

                1266    1168     540     388      82      12
 
BanII G_rGCy'C

Cuts at:      0     150     224     365     735    1437    1503    1626    1815 
   Size:        150      74     141     370     702      66     123     189

Cuts at:   1815    2182    2390    3456 
   Size:        367     208    1066

  Fragments arranged by size: 

                1066     702     370     367     208     189     150     141
                 123      74      66
 
BbeI G_GCGC'C

Cuts at:      0    1364    2532    3456 
   Size:       1364    1168     924

  Fragments arranged by size: 

                1364    1168     924
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     970    2061    3456 
   Size:        970    1091    1395

  Fragments arranged by size: 

                1395    1091     970
 
BbvCI CC'TCA_GC

Cuts at:      0     299    2338    3456 
   Size:        299    2039    1118

  Fragments arranged by size: 

                2039    1118     299
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1338    1372    3321    3355    3456 
   Size:       1338      34    1949      34     101

  Fragments arranged by size: 

                1949    1338     101      34      34
 
BclI T'GATC_A

Cuts at:      0     927    1917    3456 
   Size:        927     990    1539

  Fragments arranged by size: 

                1539     990     927
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1621    2925    3456 
   Size:       1621    1304     531

  Fragments arranged by size: 

                1621    1304     531
 
BglII A'GATC_T

Cuts at:      0     992    3212    3456 
   Size:        992    2220     244

  Fragments arranged by size: 

                2220     992     244
 
BlpI GC'TnA_GC

Cuts at:      0    1096    3035    3456 
   Size:       1096    1939     421

  Fragments arranged by size: 

                1939    1096     421
 
Bme1580I G_kGCm'C

Cuts at:      0     329    1269    1351    1437    1483    1626    1823    2390 
   Size:        329     940      82      86      46     143     197     567

Cuts at:   2390    2921    3456 
   Size:        531     535

  Fragments arranged by size: 

                 940     567     535     531     329     197     143      86
                  82      46
 
BmgBI CAC'GTC

Cuts at:      0    1395    1611    3456 
   Size:       1395     216    1845

  Fragments arranged by size: 

                1845    1395     216
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1128    2167    3456 
   Size:       1128    1039    1289

  Fragments arranged by size: 

                1289    1128    1039
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     534    1978    2565    2997    3456 
   Size:        534    1444     587     432     459

  Fragments arranged by size: 

                1444     587     534     459     432
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1723    2485    2770    3456 
   Size:       1723     762     285     686

  Fragments arranged by size: 

                1723     762     686     285
 
Bpu10I CC'TnA_GC

Cuts at:      0     299    1834    2338    3456 
   Size:        299    1535     504    1118

  Fragments arranged by size: 

                1535    1118     504     299
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419    2674    3456 
   Size:        419    2255     782

  Fragments arranged by size: 

                2255     782     419
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2128    2805    3274    3456 
   Size:       2128     677     469     182

  Fragments arranged by size: 

                2128     677     469     182
 
BsaAI yAC'GTr

Cuts at:      0     350     933    1824    2990    3456 
   Size:        350     583     891    1166     466

  Fragments arranged by size: 

                1166     891     583     466     350
 
BsaHI Gr'CG_yC

Cuts at:      0    1361    2529    3456 
   Size:       1361    1168     927

  Fragments arranged by size: 

                1361    1168     927
 
BsaWI w'CCGG_w

Cuts at:      0     587    2483    3456 
   Size:        587    1896     973

  Fragments arranged by size: 

                1896     973     587
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3338    3368    3456 
   Size:       3338      30      88

  Fragments arranged by size: 

                3338      88      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1648    2541    2677    2901    3456 
   Size:       1648     893     136     224     555

  Fragments arranged by size: 

                1648     893     555     224     136
 
BseYI C'CCAG_C

Cuts at:      0    1578    1841    3456 
   Size:       1578     263    1615

  Fragments arranged by size: 

                1615    1578     263
 
BsiEI CG_ry'CG

Cuts at:      0    1004    2049    3456 
   Size:       1004    1045    1407

  Fragments arranged by size: 

                1407    1045    1004
 
BsiHKAI G_wGCw'C

Cuts at:      0     329    1102    1981    2584    3205    3456 
   Size:        329     773     879     603     621     251

  Fragments arranged by size: 

                 879     773     621     603     329     251
 
BsmI GAATG_Cn'

Cuts at:      0     747    3456 
   Size:        747    2709
 
Bsp1286I G_dGCh'C

Cuts at:      0     150     224     329     365     735    1102    1269    1351 
   Size:        150      74     105      36     370     367     167      82

Cuts at:   1351    1437    1483    1503    1626    1815    1823    1981    2182 
   Size:         86      46      20     123     189       8     158     201

Cuts at:   2182    2390    2584    2921    3205    3456 
   Size:        208     194     337     284     251

  Fragments arranged by size: 

                 370     367     337     284     251     208     201     194
                 189     167     158     150     123     105      86      82
                  74      46      36      20       8
 
BspEI T'CCGG_A

Cuts at:      0     587    3456 
   Size:        587    2869
 
BspHI T'CATG_A

Cuts at:      0    2357    3456 
   Size:       2357    1099
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     225    3456 
   Size:        225    3231
 
BsrBI CCG'CTC

Cuts at:      0    3321    3456 
   Size:       3321     135
 
BsrDI GCAATG_nn'

Cuts at:      0      77     449    2319    3456 
   Size:         77     372    1870    1137

  Fragments arranged by size: 

                1870    1137     372      77
 
BsrFI r'CCGG_y

Cuts at:      0    2757    3456 
   Size:       2757     699
 
BsrGI T'GTAC_A

Cuts at:      0    3380    3456 
   Size:       3380      76
 
BssSI C'ACGA_G

Cuts at:      0     923    2374    3456 
   Size:        923    1451    1082

  Fragments arranged by size: 

                1451    1082     923
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1827    2588    3456 
   Size:       1827     761     868

  Fragments arranged by size: 

                1827     868     761
 
BstEII G'GTnAC_C

Cuts at:      0    2451    2628    2784    2961    3456 
   Size:       2451     177     156     177     495

  Fragments arranged by size: 

                2451     495     177     177     156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     138    1747    1890    2904    3456 
   Size:        138    1609     143    1014     552

  Fragments arranged by size: 

                1609    1014     552     143     138
 
BstYI r'GATC_y

Cuts at:      0     127     379     752     992    1678    2006    3212    3456 
   Size:        127     252     373     240     686     328    1206     244

  Fragments arranged by size: 

                1206     686     373     328     252     244     240     127
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     818    1674    3456 
   Size:        818     856    1782

  Fragments arranged by size: 

                1782     856     818
 
BtgI C'CryG_G

Cuts at:      0     242     683    1388    2793    3053    3456 
   Size:        242     441     705    1405     260     403

  Fragments arranged by size: 

                1405     705     441     403     260     242
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     891    1312    3054    3456 
   Size:        891     421    1742     402

  Fragments arranged by size: 

                1742     891     421     402
 
BtsI GCAGTG_nn'

Cuts at:      0     345     882    1516    3456 
   Size:        345     537     634    1940

  Fragments arranged by size: 

                1940     634     537     345
 
DraIII CAC_nnn'GTG

Cuts at:      0    1762    3456 
   Size:       1762    1694
 
EaeI y'GGCC_r

Cuts at:      0     680    1613    2046    2245    3456 
   Size:        680     933     433     199    1211

  Fragments arranged by size: 

                1211     933     680     433     199
 
EagI C'GGCC_G

Cuts at:      0    2046    3456 
   Size:       2046    1410
 
EarI CTCTTCn'nnn_

Cuts at:      0    1969    2281    2572    3456 
   Size:       1969     312     291     884

  Fragments arranged by size: 

                1969     884     312     291
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     217     516     684    2272    3456 
   Size:        217     299     168    1588    1184

  Fragments arranged by size: 

                1588    1184     299     217     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     599     982    1047    1723    2334    2485    2770 
   Size:        106     493     383      65     676     611     151     285

Cuts at:   2770    3456 
   Size:        686

  Fragments arranged by size: 

                 686     676     611     493     383     285     151     106
                  65
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1488    3456 
   Size:       1488    1968
 
EcoO109I rG'GnC_Cy

Cuts at:      0     237     417    1373    1433    1434    1622    1623    1773 
   Size:        237     180     956      60       1     188       1     150

Cuts at:   1773    1838    2169    2387    2570    3048    3269    3303    3433 
   Size:         65     331     218     183     478     221      34     130

Cuts at:   3433    3456 
   Size:         23

  Fragments arranged by size: 

                 956     478     331     237     221     218     188     183
                 180     150     130      65      60      34      23       1
                   1
 
EcoRI G'AATT_C

Cuts at:      0     583    2740    3124    3456 
   Size:        583    2157     384     332

  Fragments arranged by size: 

                2157     583     384     332
 
EcoRV GAT'ATC

Cuts at:      0    1276    3456 
   Size:       1276    2180
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     762    3024    3408    3456 
   Size:        762    2262     384      48

  Fragments arranged by size: 

                2262     762     384      48
 
HaeII r_GCGC'y

Cuts at:      0    1070    1364    2514    2532    3009    3456 
   Size:       1070     294    1150      18     477     447

  Fragments arranged by size: 

                1150    1070     477     447     294      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      24      56     821     853    1670    1702    2641    2673 
   Size:         24      32     765      32     817      32     939      32

Cuts at:   2673    3338    3370    3456 
   Size:        665      32      86

  Fragments arranged by size: 

                 939     817     765     665      86      32      32      32
                  32      32      24
 
HincII GTy'rAC

Cuts at:      0      23    3456 
   Size:         23    3433
 
HpaI GTT'AAC

Cuts at:      0      23    3456 
   Size:         23    3433
 
KasI G'GCGC_C

Cuts at:      0    1360    2528    3456 
   Size:       1360    1168     928

  Fragments arranged by size: 

                1360    1168     928
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     407    1355    2769    3047    3456 
   Size:        123     284     948    1414     278     409

  Fragments arranged by size: 

                1414     948     409     284     278     123
 
MscI TGG'CCA

Cuts at:      0    1615    3456 
   Size:       1615    1841
 
MslI CAynn'nnrTG

Cuts at:      0     352     856    1171    1318    2560    3456 
   Size:        352     504     315     147    1242     896

  Fragments arranged by size: 

                1242     896     504     352     315     147
 
MspA1I CmG'CkG

Cuts at:      0     303     660    1707    2588    3208    3456 
   Size:        303     357    1047     881     620     248

  Fragments arranged by size: 

                1047     881     620     357     303     248
 
NarI GG'CG_CC

Cuts at:      0    1361    2529    3456 
   Size:       1361    1168     927

  Fragments arranged by size: 

                1361    1168     927
 
NcoI C'CATG_G

Cuts at:      0    2793    3456 
   Size:       2793     663
 
NdeI CA'TA_TG

Cuts at:      0    2944    3456 
   Size:       2944     512
 
NspI r_CATG'y

Cuts at:      0     261    1323    2721    3456 
   Size:        261    1062    1398     735

  Fragments arranged by size: 

                1398    1062     735     261
 
PasI CC'CwG_GG

Cuts at:      0     264    1490    1547    1815    1883    3456 
   Size:        264    1226      57     268      68    1573

  Fragments arranged by size: 

                1573    1226     268     264      68      57
 
PciI A'CATG_T

Cuts at:      0     257    3456 
   Size:        257    3199
 
PfoI T'CCnGG_A

Cuts at:      0    2612    3116    3456 
   Size:       2612     504     340

  Fragments arranged by size: 

                2612     504     340
 
PmlI CAC'GTG

Cuts at:      0     933    1824    3456 
   Size:        933     891    1632

  Fragments arranged by size: 

                1632     933     891
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2497    2529    3456 
   Size:       2497      32     927

  Fragments arranged by size: 

                2497     927      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     237    1773    1838    2169    3269    3303    3433    3456 
   Size:        237    1536      65     331    1100      34     130      23

  Fragments arranged by size: 

                1536    1100     331     237     130      65      34      23
            
 
PshAI GACnn'nnGTC

Cuts at:      0    2107    2449    3456 
   Size:       2107     342    1007

  Fragments arranged by size: 

                2107    1007     342
 
PspOMI G'GGCC_C

Cuts at:      0    1433    1622    2386    3456 
   Size:       1433     189     764    1070

  Fragments arranged by size: 

                1433    1070     764     189
 
PstI C_TGCA'G

Cuts at:      0     236    2593    3456 
   Size:        236    2357     863

  Fragments arranged by size: 

                2357     863     236
 
PvuI CG_AT'CG

Cuts at:      0    1004    3456 
   Size:       1004    2452
 
PvuII CAG'CTG

Cuts at:      0     303     660    2588    3208    3456 
   Size:        303     357    1928     620     248

  Fragments arranged by size: 

                1928     620     357     303     248
 
SanDI GG'GwC_CC

Cuts at:      0    3433    3456 
   Size:       3433      23
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1969    2572    3456 
   Size:       1969     603     884

  Fragments arranged by size: 

                1969     884     603
 
SbfI CC_TGCA'GG

Cuts at:      0     236    3456 
   Size:        236    3220
 
ScaI AGT'ACT

Cuts at:      0     574    1850    3456 
   Size:        574    1276    1606

  Fragments arranged by size: 

                1606    1276     574
 
SexAI A'CCwGG_T

Cuts at:      0    1418    3456 
   Size:       1418    2038
 
SfcI C'TryA_G

Cuts at:      0     201     232    1805    2146    2439    2589    3456 
   Size:        201      31    1573     341     293     150     867

  Fragments arranged by size: 

                1573     867     341     293     201     150      31
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1621    3456 
   Size:       1621    1835
 
SfoI GGC'GCC

Cuts at:      0    1362    2530    3456 
   Size:       1362    1168     926

  Fragments arranged by size: 

                1362    1168     926
 
SmaI CCC'GGG

Cuts at:      0    2392    3440    3456 
   Size:       2392    1048      16

  Fragments arranged by size: 

                2392    1048      16
 
SmlI C'TyrA_G

Cuts at:      0     434    2689    2982    3456 
   Size:        434    2255     293     474

  Fragments arranged by size: 

                2255     474     434     293
 
StyI C'CwwG_G

Cuts at:      0     794    2670    2793    2999    3456 
   Size:        794    1876     123     206     457

  Fragments arranged by size: 

                1876     794     457     206     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1366    2371    3456 
   Size:       1366    1005    1085

  Fragments arranged by size: 

                1366    1085    1005
 
TatI w'GTAC_w

Cuts at:      0     572    1848    3380    3456 
   Size:        572    1276    1532      76

  Fragments arranged by size: 

                1532    1276     572      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     119     151    3456 
   Size:        119      32    3305

  Fragments arranged by size: 

                3305     119      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1420    3456 
   Size:       1420    2036
 
XhoI C'TCGA_G

Cuts at:      0    2982    3456 
   Size:       2982     474
 
XmaI C'CCGG_G

Cuts at:      0    2390    3438    3456 
   Size:       2390    1048      18

  Fragments arranged by size: 

                2390    1048      18
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII     HpaI
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NspI     PasI     PciI     PfoI     PmlI     PpiI    PpuMI    PshAI
   PspOMI     PstI     PvuI    PvuII    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     StyI    TaqII
     TatI     TstI  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BciVI    BfrBI     BmtI    BsaBI
     BsgI    BsiWI    BsmBI   BssHII    BstBI  BstZ17I     ClaI    CspCI
     DraI     DrdI  EcoICRI     FseI     FspI    FspAI  HindIII     KpnI
     MfeI     MluI     NaeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PsiI    PspXI     PsrI    RsrII     SacI
    SacII     SalI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     XbaI     XcmI     XmnI     ZraI
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