Restriction map for pF1KB3027
 (Circular) (Six-base) MAPSORT of: pF1KB3027.seq  Check: 9624  from: 1  to: 6755



pF1KB3027  6755 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1270    1805    2453    1270 
   Size:        535     648    5572

  Fragments arranged by size: 

                5572     648     535
 
AccI GT'mk_AC

Cuts at:    200    3068    3793     200 
   Size:       2868     725    3162

  Fragments arranged by size: 

                3162    2868     725
 
Acc65I G'GTAC_C

Cuts at:   2091    3771    2091 
   Size:       1680    5075
 
AclI AA'CG_TT

Cuts at:   6595    6595 
   Size:       6755
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1765    2049    2414    3012    3128    4580    5012    5759    6027 
   Size:        284     365     598     116    1452     432     747     268

Cuts at:   6027    1765 
   Size:       2493

  Fragments arranged by size: 

                2493    1452     747     598     432     365     284     268
                 116
 
AfeI AGC'GCT

Cuts at:   3138    3138 
   Size:       6755
 
AflIII A'CryG_T

Cuts at:    989    2738    4107    5211    6188     989 
   Size:       1749    1369    1104     977    1556

  Fragments arranged by size: 

                1749    1556    1369    1104     977
 
AgeI A'CCGG_T

Cuts at:    704    5143     704 
   Size:       4439    2316
 
AhdI GACnn_n'nnGTC

Cuts at:   3111    3111 
   Size:       6755
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2300    3727    2300 
   Size:       1427    5328
 
AlwNI CAG_nnn'CTG

Cuts at:   1053    1633    1725    1736    2216    3109    3244    3576    4060 
   Size:        580      92      11     480     893     135     332     484

Cuts at:   4060    5627    1053 
   Size:       1567    2181

  Fragments arranged by size: 

                2181    1567     893     580     484     480     332     135
                  92      11
 
ApaI G_GGCC'C

Cuts at:   1155    1857    2897    1155 
   Size:        702    1040    5013

  Fragments arranged by size: 

                5013    1040     702
 
ApaLI G'TGCA_C

Cuts at:   1006    5525    1006 
   Size:       4519    2236
 
ApoI r'AATT_y

Cuts at:   2172    3759    6108    6364    2172 
   Size:       1587    2349     256    2563

  Fragments arranged by size: 

                2563    2349    1587     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6755
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6755
 
AvaI C'yCGr_G

Cuts at:    484    2625    3200    3434    3521    3775     484 
   Size:       2141     575     234      87     254    3464

  Fragments arranged by size: 

                3464    2141     575     254     234      87
 
BamHI G'GATC_C

Cuts at:   1175    3780    1175 
   Size:       2605    4150
 
BanI G'GyrC_C

Cuts at:    302    1120    1396    1405    2091    2306    3771    4185    4433 
   Size:        818     276       9     686     215    1465     414     248

Cuts at:   4433    4468     302 
   Size:         35    2589

  Fragments arranged by size: 

                2589    1465     818     686     414     276     248     215
                  35       9
 
BanII G_rGCy'C

Cuts at:   1155    1288    1546    1857    1911    2897    2950    2968    3693 
   Size:        133     258     311      54     986      53      18     725

Cuts at:   3693    3769    4799    1155 
   Size:         76    1030    3111

  Fragments arranged by size: 

                3111    1030     986     725     311     258     133      76
                  54      53      18
 
BbeI G_GCGC'C

Cuts at:    306    1400    2310    4189    4437     306 
   Size:       1094     910    1879     248    2624

  Fragments arranged by size: 

                2624    1879    1094     910     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    136     811    1197    2812    3002     136 
   Size:        675     386    1615     190    3889

  Fragments arranged by size: 

                3889    1615     675     386     190
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    703     737     889     923    2479    2513     703 
   Size:         34     152      34    1556      34    4945

  Fragments arranged by size: 

                4945    1556     152      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3225    4653    5414    6755    3225 
   Size:       1428     761    1341    3225

  Fragments arranged by size: 

                3225    1428    1341     761
 
BclI T'GATC_A

Cuts at:   2540    3413    4275    2540 
   Size:        873     862    5020

  Fragments arranged by size: 

                5020     873     862
 
BfrBI ATG'CAT

Cuts at:   2349    2349 
   Size:       6755
 
BglI GCCn_nnn'nGGC

Cuts at:   1354    2826    2835    2879    1354 
   Size:       1472       9      44    5230

  Fragments arranged by size: 

                5230    1472      44       9
 
BglII A'GATC_T

Cuts at:    836    4270    6307     836 
   Size:       3434    2037    1284

  Fragments arranged by size: 

                3434    2037    1284
 
BlpI GC'TnA_GC

Cuts at:   3867    3867 
   Size:       6755
 
Bme1580I G_kGCm'C

Cuts at:    178    1010    1101    1108    1155    1649    1857    2897    3208 
   Size:        832      91       7      47     494     208    1040     311

Cuts at:   3208    3271    3476    4380    4473    5529     178 
   Size:         63     205     904      93    1056    1404

  Fragments arranged by size: 

                1404    1056    1040     904     832     494     311     208
                 205      93      91      63      47       7
 
BmgBI CAC'GTC

Cuts at:   2599    2599 
   Size:       6755
 
BmrI ACTGGGnnnn_n'

Cuts at:   1860    2951    3967    4048    4383    6527    1860 
   Size:       1091    1016      81     335    2144    2088

  Fragments arranged by size: 

                2144    2088    1091    1016     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    641    3252    3276    3547    5122     641 
   Size:       2611      24     271    1575    2274

  Fragments arranged by size: 

                2611    2274    1575     271      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    883    1439    1560    2390    2449    2497    3502    3658     883 
   Size:        556     121     830      59      48    1005     156    3980

  Fragments arranged by size: 

                3980    1005     830     556     156     121      59      48
            
 
Bpu10I CC'TnA_GC

Cuts at:   1287    2609    1287 
   Size:       1322    5433
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1855    2206    3931    4810    5302    5600    1855 
   Size:        351    1725     879     492     298    3010

  Fragments arranged by size: 

                3010    1725     879     492     351     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    178     354    3727    6459     178 
   Size:        176    3373    2732     474

  Fragments arranged by size: 

                3373    2732     474     176
 
BsaAI yAC'GTr

Cuts at:   4108    4108 
   Size:       6755
 
BsaBI GATnn'nnATC

Cuts at:      6    4269    6306       6 
   Size:       4263    2037     455

  Fragments arranged by size: 

                4263    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    303    1397    2307    4186    4434    5136    6632     303 
   Size:       1094     910    1879     248     702    1496     426

  Fragments arranged by size: 

                1879    1496    1094     910     702     426     248
 
BsaWI w'CCGG_w

Cuts at:    704    3149    3944    4465    5143    5417    5564    5975     704 
   Size:       2445     795     521     678     274     147     411    1484

  Fragments arranged by size: 

                2445    1484     795     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    856     886     856 
   Size:         30    6725
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    502     742     778    1516    1531    1534    2203    2710     502 
   Size:        240      36     738      15       3     669     507    4547

  Fragments arranged by size: 

                4547     738     669     507     240      36      15       3
            
 
BseYI C'CCAG_C

Cuts at:    315     334     392    1148    1349    2869    2900    3405    4180 
   Size:         19      58     756     201    1520      31     505     775

Cuts at:   4180    4938    5515    6011     315 
   Size:        758     577     496    1059

  Fragments arranged by size: 

                1520    1059     775     758     756     577     505     496
                 201      58      31      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1711    1711 
   Size:       6755
 
BsiEI CG_ry'CG

Cuts at:    104    4297    4343    5551     104 
   Size:       4193      46    1208    1308

  Fragments arranged by size: 

                4193    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    622    1010    1235    1546    1911    2294    2968    3769    4547 
   Size:        388     225     311     365     383     674     801     778

Cuts at:   4547    4737    5529     622 
   Size:        190     792    1848

  Fragments arranged by size: 

                1848     801     792     778     674     388     383     365
                 311     225     190
 
BsmI GAATG_Cn'

Cuts at:   2502    2502 
   Size:       6755
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1951    2332    2584    1951 
   Size:        381     252    6122

  Fragments arranged by size: 

                6122     381     252
 
Bsp1286I G_dGCh'C

Cuts at:    178     622    1010    1101    1108    1155    1235    1288    1546 
   Size:        444     388      91       7      47      80      53     258

Cuts at:   1546    1649    1857    1911    2294    2897    2950    2968    3208 
   Size:        103     208      54     383     603      53      18     240

Cuts at:   3208    3271    3476    3693    3769    4380    4473    4547    4737 
   Size:         63     205     217      76     611      93      74     190

Cuts at:   4737    4799    5529     178 
   Size:         62     730    1404

  Fragments arranged by size: 

                1404     730     611     603     444     388     383     258
                 240     217     208     205     190     103      93      91
                  80      76      74      63      62      54      53      53
                  47      18       7
 
BspEI T'CCGG_A

Cuts at:   5975    5975 
   Size:       6755
 
BspHI T'CATG_A

Cuts at:    788    2912    5931     788 
   Size:       2124    3019    1612

  Fragments arranged by size: 

                3019    2124    1612
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    196    1270    1805    2453    3805    4019    4321    4702     196 
   Size:       1074     535     648    1352     214     302     381    2249

  Fragments arranged by size: 

                2249    1352    1074     648     535     381     302     214
            
 
BsrBI CCG'CTC

Cuts at:     50    2415    2481    2522    2613    5047    5101    6585    6750 
   Size:       2365      66      41      91    2434      54    1484     165

Cuts at:   6750      50 
   Size:         55

  Fragments arranged by size: 

                2434    2365    1484     165      91      66      55      54
                  41
 
BsrDI GCAATG_nn'

Cuts at:    951     975    1771    4667     951 
   Size:         24     796    2896    3039

  Fragments arranged by size: 

                3039    2896     796      24
 
BsrFI r'CCGG_y

Cuts at:    704    2073    2834    4753    4934    5143     704 
   Size:       1369     761    1919     181     209    2316

  Fragments arranged by size: 

                2316    1919    1369     761     209     181
 
BssSI C'ACGA_G

Cuts at:   1082    1918    3370    5026    5384    1082 
   Size:        836    1452    1656     358    2453

  Fragments arranged by size: 

                2453    1656    1452     836     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1304    1725    3576    4132    1304 
   Size:        421    1851     556    3927

  Fragments arranged by size: 

                3927    1851     556     421
 
BstBI TT'CG_AA

Cuts at:   5116    5116 
   Size:       6755
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114    1250    1357     114 
   Size:       1136     107    5512

  Fragments arranged by size: 

                5512    1136     107
 
BstYI r'GATC_y

Cuts at:      1     836    1175    1604    3780    4247    4270    4605    4851 
   Size:        835     339     429    2176     467      23     335     246

Cuts at:   4851    5863    5949    5961    6307       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                2176    1012     835     467     449     429     346     339
                 335     246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   3069    3069 
   Size:       6755
 
Bsu36I CC'TnA_GG

Cuts at:   1040    1181    3237    1040 
   Size:        141    2056    4558

  Fragments arranged by size: 

                4558    2056     141
 
BtgI C'CryG_G

Cuts at:    107    1982    2123    2874    3263    3276    3453     107 
   Size:       1875     141     751     389      13     177    3409

  Fragments arranged by size: 

                3409    1875     751     389     177     141      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4705    4886    5049    4705 
   Size:        181     163    6411

  Fragments arranged by size: 

                6411     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2267    2560    3231    3577    3735    3998    2267 
   Size:        293     671     346     158     263    5024

  Fragments arranged by size: 

                5024     671     346     293     263     158
 
DraI TTT'AAA

Cuts at:   3754    3754 
   Size:       6755
 
DraIII CAC_nnn'GTG

Cuts at:   2530    2530 
   Size:       6755
 
DrdI GACnn_nn'nnGTC

Cuts at:   4461    5319    4461 
   Size:        858    5897
 
EaeI y'GGCC_r

Cuts at:    420     688    1001    3273    4340    4514    4905    4932    6277 
   Size:        268     313    2272    1067     174     391      27    1345

Cuts at:   6277     420 
   Size:        898

  Fragments arranged by size: 

                2272    1345    1067     898     391     313     268     174
                  27
 
EagI C'GGCC_G

Cuts at:   4340    4340 
   Size:       6755
 
EarI CTCTTCn'nnn_

Cuts at:    985    1699    2405    3049    3298    3550    4778    4988     985 
   Size:        714     706     644     249     252    1228     210    2752

  Fragments arranged by size: 

                2752    1228     714     706     644     252     249     210
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2284    2583    4963    5273    5419    6565    2284 
   Size:        299    2380     310     146    1146    2474

  Fragments arranged by size: 

                2474    2380    1146     310     299     146
 
EcoICRI GAG'CTC

Cuts at:   1544    1909    2966    3767    1544 
   Size:        365    1057     801    4532

  Fragments arranged by size: 

                4532    1057     801     365
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    883    1439    1560    1765    2049    2390    2414    2449    2497 
   Size:        556     121     205     284     341      24      35      48

Cuts at:   2497    3012    3128    3502    3658    4580    5012    5759    6027 
   Size:        515     116     374     156     922     432     747     268

Cuts at:   6027     883 
   Size:       1611

  Fragments arranged by size: 

                1611     922     747     556     515     432     374     341
                 284     268     205     156     121     116      48      35
                  24
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3622    3684    3622 
   Size:         62    6693
 
EcoO109I rG'GnC_Cy

Cuts at:    492    1292    1767    1854    2731    2893    2894    3615    3894 
   Size:        800     475      87     877     162       1     721     279

Cuts at:   3894     492 
   Size:       3353

  Fragments arranged by size: 

                3353     877     800     721     475     279     162      87
                   1
 
EcoRI G'AATT_C

Cuts at:   3759    3759 
   Size:       6755
 
EcoRV GAT'ATC

Cuts at:    786     786 
   Size:       6755
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2166    3078    4774    2166 
   Size:        912    1696    4147

  Fragments arranged by size: 

                4147    1696     912
 
FseI GG_CCGG'CC

Cuts at:   2838    2838 
   Size:       6755
 
FspI TGC'GCA

Cuts at:   4536    4536 
   Size:       6755
 
HaeII r_GCGC'y

Cuts at:    306    1149    1400    2310    3140    4189    4437    5459    6438 
   Size:        843     251     910     830    1049     248    1022     979

Cuts at:   6438     306 
   Size:        623

  Fragments arranged by size: 

                1049    1022     979     910     843     830     623     251
                 248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    448     480     856     888    3097    3129    3331    3363    3405 
   Size:         32     376      32    2209      32     202      32      42

Cuts at:   3405    3437    4597    4627    4629    4659     448 
   Size:         32    1160      30       2      30    2544

  Fragments arranged by size: 

                2544    2209    1160     376     202      42      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   3794    3794 
   Size:       6755
 
HindIII A'AGCT_T

Cuts at:   2572    2572 
   Size:       6755
 
KasI G'GCGC_C

Cuts at:    302    1396    2306    4185    4433     302 
   Size:       1094     910    1879     248    2624

  Fragments arranged by size: 

                2624    1879    1094     910     248
 
KpnI G_GTAC'C

Cuts at:   2095    3775    2095 
   Size:       1680    5075
 
MluI A'CGCG_T

Cuts at:   6188    6188 
   Size:       6755
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    611     781    3677    5426    5610     611 
   Size:        170    2896    1749     184    1756

  Fragments arranged by size: 

                2896    1756    1749     184     170
 
MscI TGG'CCA

Cuts at:   4516    4516 
   Size:       6755
 
MslI CAynn'nnrTG

Cuts at:    112     171     605    1372    1987    3469    4744    4871     112 
   Size:         59     434     767     615    1482    1275     127    1996

  Fragments arranged by size: 

                1996    1482    1275     767     615     434     127      59
            
 
MspA1I CmG'CkG

Cuts at:    535    1884    1995    2812    3032    3278    3354    3866    4180 
   Size:       1349     111     817     220     246      76     512     314

Cuts at:   4180    4540    5553    5798     535 
   Size:        360    1013     245    1492

  Fragments arranged by size: 

                1492    1349    1013     817     512     360     314     246
                 245     220     111      76
 
NaeI GCC'GGC

Cuts at:   2836    4936    2836 
   Size:       2100    4655
 
NarI GG'CG_CC

Cuts at:    303    1397    2307    4186    4434     303 
   Size:       1094     910    1879     248    2624

  Fragments arranged by size: 

                2624    1879    1094     910     248
 
NcoI C'CATG_G

Cuts at:    107    1982    2123    3453     107 
   Size:       1875     141    1330    3409

  Fragments arranged by size: 

                3409    1875    1330     141
 
NgoMIV G'CCGG_C

Cuts at:   2834    4934    2834 
   Size:       2100    4655
 
NsiI A_TGCA'T

Cuts at:   2351    2351 
   Size:       6755
 
NspI r_CATG'y

Cuts at:    993    1346    2349    2742    3808    4308    5215     993 
   Size:        353    1003     393    1066     500     907    2533

  Fragments arranged by size: 

                2533    1066    1003     907     500     393     353
 
PciI A'CATG_T

Cuts at:    989    2738    5211     989 
   Size:       1749    2473    2533

  Fragments arranged by size: 

                2533    2473    1749
 
PflMI CCAn_nnn'nTGG

Cuts at:    399    2364    3022    3294     399 
   Size:       1965     658     272    3860

  Fragments arranged by size: 

                3860    1965     658     272
 
PfoI T'CCnGG_A

Cuts at:   1576    1576 
   Size:       6755
 
PmeI GTTT'AAAC

Cuts at:   3754    3754 
   Size:       6755
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5894    5926    5894 
   Size:         32    6723
 
PpuMI rG'GwC_Cy

Cuts at:   1767    2731    3615    1767 
   Size:        964     884    4907

  Fragments arranged by size: 

                4907     964     884
 
PspOMI G'GGCC_C

Cuts at:   1151    1853    2893    1151 
   Size:        702    1040    5013

  Fragments arranged by size: 

                5013    1040     702
 
PspXI vC'TCGA_Gb

Cuts at:   2625    2625 
   Size:       6755
 
PstI C_TGCA'G

Cuts at:    726    1073    1790    3085    3698    3802    4487     726 
   Size:        347     717    1295     613     104     685    2994

  Fragments arranged by size: 

                2994    1295     717     685     613     347     104
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6755
 
PvuII CAG'CTG

Cuts at:    535    1995    2812    4180    4540     535 
   Size:       1460     817    1368     360    2750

  Fragments arranged by size: 

                2750    1460    1368     817     360
 
RsrII CG'GwC_CG

Cuts at:   4950    4950 
   Size:       6755
 
SacI G_AGCT'C

Cuts at:   1546    1911    2968    3769    1546 
   Size:        365    1057     801    4532

  Fragments arranged by size: 

                4532    1057     801     365
 
SacII CC_GC'GG

Cuts at:   3279    3279 
   Size:       6755
 
SalI G'TCGA_C

Cuts at:   3792    3792 
   Size:       6755
 
SanDI GG'GwC_CC

Cuts at:   1767    2731    1767 
   Size:        964    5791
 
SapI GCTCTTCn'nnn_

Cuts at:   1699    2405    4778    4988    1699 
   Size:        706    2373     210    3466

  Fragments arranged by size: 

                3466    2373     706     210
 
SbfI CC_TGCA'GG

Cuts at:   3802    3802 
   Size:       6755
 
ScaI AGT'ACT

Cuts at:   6272    6272 
   Size:       6755
 
SfcI C'TryA_G

Cuts at:     33     722    1069    1786    3081    3694    3798    4483    5476 
   Size:        689     347     717    1295     613     104     685     993

Cuts at:   5476    5667      33 
   Size:        191    1121

  Fragments arranged by size: 

                1295    1121     993     717     689     685     613     347
                 191     104
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2835    2835 
   Size:       6755
 
SfoI GGC'GCC

Cuts at:    304    1398    2308    4187    4435     304 
   Size:       1094     910    1879     248    2624

  Fragments arranged by size: 

                2624    1879    1094     910     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6755
 
SmaI CCC'GGG

Cuts at:   3777    3777 
   Size:       6755
 
SmlI C'TyrA_G

Cuts at:   1870    2221    2625    3910    4825    5317    5579    1870 
   Size:        351     404    1285     915     492     262    3046

  Fragments arranged by size: 

                3046    1285     915     492     404     351     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6755
 
SphI G_CATG'C

Cuts at:   2349    3808    4308    2349 
   Size:       1459     500    4796

  Fragments arranged by size: 

                4796    1459     500
 
StuI AGG'CCT

Cuts at:   2726    3256    2726 
   Size:        530    6225
 
StyI C'CwwG_G

Cuts at:    107    1565    1982    2027    2123    2234    3453    3889    4243 
   Size:       1458     417      45      96     111    1219     436     354

Cuts at:   4243     107 
   Size:       2619

  Fragments arranged by size: 

                2619    1458    1219     436     417     354     111      96
                  45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    348     872    3592    4338    4471    4930    5139     348 
   Size:        524    2720     746     133     459     209    1964

  Fragments arranged by size: 

                2720    1964     746     524     459     209     133
 
TatI w'GTAC_w

Cuts at:   3669    6270    3669 
   Size:       2601    4154
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1589    1621    3358    3390    6272    6304    1589 
   Size:         32    1737      32    2882      32    2040

  Fragments arranged by size: 

                2882    2040    1737      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   4552    4552 
   Size:       6755
 
XbaI T'CTAG_A

Cuts at:   3786    3786 
   Size:       6755
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1247    2365    3588    4250    1247 
   Size:       1118    1223     662    3752

  Fragments arranged by size: 

                3752    1223    1118     662
 
XhoI C'TCGA_G

Cuts at:   2625    2625 
   Size:       6755
 
XmaI C'CCGG_G

Cuts at:   3775    3775 
   Size:       6755
 
XmnI GAAnn'nnTTC

Cuts at:    133    3058     133 
   Size:       2925    3830
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NsiI     NspI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII    AflII     AleI     AloI     AscI    AvrII     BaeI    BbvCI
     BmtI    BsiWI    BsrGI   BssHII   BstEII     ClaI    CspCI    FspAI
     HpaI     MfeI     NdeI     NheI     NotI     NruI     PacI     PasI
     PmlI    PshAI     PsiI     PsrI    SexAI    SgrAI    SnaBI     SrfI
     SspI     SwaI     ZraI
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