Restriction map for pF1KB3017
 (Linear) (Six-base) MAPSORT of: KIBB3017.seq  Check: 707  from: 1  to: 2394



KIBB3017.orf

 With 182 enzymes: SgfI * 

                        July 23, 2007 10:03  ..

AccI GT'mk_AC

Cuts at:      0    1064    2394 
   Size:       1064    1330
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     794     845    1953    2394 
   Size:        794      51    1108     441

  Fragments arranged by size: 

                1108     794     441      51
 
AfeI AGC'GCT

Cuts at:      0     664    2394 
   Size:        664    1730
 
AflIII A'CryG_T

Cuts at:      0     507    2394 
   Size:        507    1887
 
AleI CACnn'nnGTG

Cuts at:      0    1463    2128    2394 
   Size:       1463     665     266

  Fragments arranged by size: 

                1463     665     266
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     928    1806    2146    2394 
   Size:        928     878     340     248

  Fragments arranged by size: 

                 928     878     340     248
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1074    2394 
   Size:       1074    1320
 
ApaI G_GGCC'C

Cuts at:      0    1377    1604    2394 
   Size:       1377     227     790

  Fragments arranged by size: 

                1377     790     227
 
AvaI C'yCGr_G

Cuts at:      0     263     520    1822    2394 
   Size:        263     257    1302     572

  Fragments arranged by size: 

                1302     572     263     257
 
AvrII C'CTAG_G

Cuts at:      0    1518    2394 
   Size:       1518     876
 
BanI G'GyrC_C

Cuts at:      0     340     355     369     740    1577    2394 
   Size:        340      15      14     371     837     817

  Fragments arranged by size: 

                 837     817     371     340      15      14
 
BanII G_rGCy'C

Cuts at:      0     296     410    1355    1377    1587    1604    1663    1835 
   Size:        296     114     945      22     210      17      59     172

Cuts at:   1835    2394 
   Size:        559

  Fragments arranged by size: 

                 945     559     296     210     172     114      59      22
                  17
 
BbeI G_GCGC'C

Cuts at:      0     344     373     744    2394 
   Size:        344      29     371    1650

  Fragments arranged by size: 

                1650     371     344      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1160    2394 
   Size:       1160    1234
 
BbvCI CC'TCA_GC

Cuts at:      0     440    1265    2394 
   Size:        440     825    1129

  Fragments arranged by size: 

                1129     825     440
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1262    1296    2394 
   Size:       1262      34    1098

  Fragments arranged by size: 

                1262    1098      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     439    2394 
   Size:        439    1955
 
BlpI GC'TnA_GC

Cuts at:      0     331    2394 
   Size:        331    2063
 
Bme1580I G_kGCm'C

Cuts at:      0     527     929    1377    1509    1604    2013    2394 
   Size:        527     402     448     132      95     409     381

  Fragments arranged by size: 

                 527     448     409     402     381     132      95
 
BmgBI CAC'GTC

Cuts at:      0    1290    2394 
   Size:       1290    1104
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     911    1224    2394 
   Size:        911     313    1170

  Fragments arranged by size: 

                1170     911     313
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1572    2394 
   Size:       1572     822
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     307     403     679     849    1107    2394 
   Size:        298       9      96     276     170     258    1287

  Fragments arranged by size: 

                1287     298     276     258     170      96       9
 
Bpu10I CC'TnA_GC

Cuts at:      0      40     440     478    1265    2394 
   Size:         40     400      38     787    1129

  Fragments arranged by size: 

                1129     787     400      40      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2136    2394 
   Size:       2136     258
 
BsaHI Gr'CG_yC

Cuts at:      0      65     341     370     741    2114    2394 
   Size:         65     276      29     371    1373     280

  Fragments arranged by size: 

                1373     371     280     276      65      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265     295     304    1405    2394 
   Size:        265      30       9    1101     989

  Fragments arranged by size: 

                1101     989     265      30       9
 
BseYI C'CCAG_C

Cuts at:      0     123     175     362     410     883    1442    2184    2217 
   Size:        123      52     187      48     473     559     742      33

Cuts at:   2217    2394 
   Size:        177

  Fragments arranged by size: 

                 742     559     473     187     177     123      52      48
                  33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     788    1629    1973    2394 
   Size:        788     841     344     421

  Fragments arranged by size: 

                 841     788     421     344
 
BsiEI CG_ry'CG

Cuts at:      0    2129    2394 
   Size:       2129     265
 
BsiHKAI G_wGCw'C

Cuts at:      0     619    1540    1663    1899    2135    2394 
   Size:        619     921     123     236     236     259

  Fragments arranged by size: 

                 921     619     259     236     236     123
 
BsmI GAATG_Cn'

Cuts at:      0    1795    2394 
   Size:       1795     599
 
Bsp1286I G_dGCh'C

Cuts at:      0     296     410     527     619     929    1355    1377    1509 
   Size:        296     114     117      92     310     426      22     132

Cuts at:   1509    1540    1587    1604    1663    1835    1899    2013    2135 
   Size:         31      47      17      59     172      64     114     122

Cuts at:   2135    2394 
   Size:        259

  Fragments arranged by size: 

                 426     310     296     259     172     132     122     117
                 114     114      92      64      59      47      31      22
                  17
 
BsrBI CCG'CTC

Cuts at:      0     330     729    2070    2394 
   Size:        330     399    1341     324

  Fragments arranged by size: 

                1341     399     330     324
 
BsrDI GCAATG_nn'

Cuts at:      0     951    2394 
   Size:        951    1443
 
BsrFI r'CCGG_y

Cuts at:      0    2237    2394 
   Size:       2237     157
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     312     531    1718    1784    1868    2394 
   Size:        312     219    1187      66      84     526

  Fragments arranged by size: 

                1187     526     312     219      84      66
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     366     657     883    1131    2184    2394 
   Size:        366     291     226     248    1053     210

  Fragments arranged by size: 

                1053     366     291     248     226     210
 
BstYI r'GATC_y

Cuts at:      0      71    2394 
   Size:         71    2323
 
Bsu36I CC'TnA_GG

Cuts at:      0    1363    2394 
   Size:       1363    1031
 
BtgI C'CryG_G

Cuts at:      0     297     345    2394 
   Size:        297      48    2049

  Fragments arranged by size: 

                2049     297      48
 
BtsI GCAGTG_nn'

Cuts at:      0     927    1054    1338    1440    1458    1470    1617    1656 
   Size:        927     127     284     102      18      12     147      39

Cuts at:   1656    1800    1845    2216    2394 
   Size:        144      45     371     178

  Fragments arranged by size: 

                 927     371     284     178     147     144     127     102
                  45      39      18      12
 
DraIII CAC_nnn'GTG

Cuts at:      0     693    1418    2394 
   Size:        693     725     976

  Fragments arranged by size: 

                 976     725     693
 
EaeI y'GGCC_r

Cuts at:      0     300    1470    1512    1985    2394 
   Size:        300    1170      42     473     409

  Fragments arranged by size: 

                1170     473     409     300      42
 
EarI CTCTTCn'nnn_

Cuts at:      0    1197    1243    2394 
   Size:       1197      46    1151

  Fragments arranged by size: 

                1197    1151      46
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     117     238    2394 
   Size:        117     121    2156

  Fragments arranged by size: 

                2156     121     117
 
EcoICRI GAG'CTC

Cuts at:      0    1661    2394 
   Size:       1661     733
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     307     403     679     794     845     849    1107 
   Size:        298       9      96     276     115      51       4     258

Cuts at:   1107    1953    2394 
   Size:        846     441

  Fragments arranged by size: 

                 846     441     298     276     258     115      96      51
                   9       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0     128    1005    1240    1374    1600    1601    1964    2394 
   Size:        128     877     235     134     226       1     363     430

  Fragments arranged by size: 

                 877     430     363     235     226     134     128       1
            
 
FspI TGC'GCA

Cuts at:      0     568    2394 
   Size:        568    1826
 
FspAI rTGC'GCAy

Cuts at:      0     568    2394 
   Size:        568    1826
 
HaeII r_GCGC'y

Cuts at:      0     237     344     373     666     744    2394 
   Size:        237     107      29     293      78    1650

  Fragments arranged by size: 

                1650     293     237     107      78      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1555    1587    2111    2143    2394 
   Size:       1555      32     524      32     251

  Fragments arranged by size: 

                1555     524     251      32      32
 
KasI G'GCGC_C

Cuts at:      0     340     369     740    2394 
   Size:        340      29     371    1654

  Fragments arranged by size: 

                1654     371     340      29
 
MluI A'CGCG_T

Cuts at:      0     507    2394 
   Size:        507    1887
 
MscI TGG'CCA

Cuts at:      0    1472    1987    2394 
   Size:       1472     515     407

  Fragments arranged by size: 

                1472     515     407
 
MslI CAynn'nnrTG

Cuts at:      0     910    1463    1760    1788    2018    2128    2394 
   Size:        910     553     297      28     230     110     266

  Fragments arranged by size: 

                 910     553     297     266     230     110      28
 
MspA1I CmG'CkG

Cuts at:      0     179     276     299     347     366     378     883    1502 
   Size:        179      97      23      48      19      12     505     619

Cuts at:   1502    1646    1694    1868    2088    2394 
   Size:        144      48     174     220     306

  Fragments arranged by size: 

                 619     505     306     220     179     174     144      97
                  48      48      23      19      12
 
NarI GG'CG_CC

Cuts at:      0     341     370     741    2394 
   Size:        341      29     371    1653

  Fragments arranged by size: 

                1653     371     341      29
 
NspI r_CATG'y

Cuts at:      0    1787    2394 
   Size:       1787     607
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      98    1878    2394 
   Size:         98    1780     516

  Fragments arranged by size: 

                1780     516      98
 
PpuMI rG'GwC_Cy

Cuts at:      0     128    1240    2394 
   Size:        128    1112    1154

  Fragments arranged by size: 

                1154    1112     128
 
PsiI TTA'TAA

Cuts at:      0    1157    2394 
   Size:       1157    1237
 
PspOMI G'GGCC_C

Cuts at:      0    1373    1600    2394 
   Size:       1373     227     794

  Fragments arranged by size: 

                1373     794     227
 
PspXI vC'TCGA_Gb

Cuts at:      0     263    2394 
   Size:        263    2131
 
PstI C_TGCA'G

Cuts at:      0     320     548     800    1017    1101    1465    1645    1651 
   Size:        320     228     252     217      84     364     180       6

Cuts at:   1651    1840    1999    2394 
   Size:        189     159     395

  Fragments arranged by size: 

                 395     364     320     252     228     217     189     180
                 159      84       6
 
PvuII CAG'CTG

Cuts at:      0     179     366     883    1502    1646    1694    2088    2394 
   Size:        179     187     517     619     144      48     394     306

  Fragments arranged by size: 

                 619     517     394     306     187     179     144      48
            
 
SacI G_AGCT'C

Cuts at:      0    1663    2394 
   Size:       1663     731
 
SacII CC_GC'GG

Cuts at:      0     300     348    2394 
   Size:        300      48    2046

  Fragments arranged by size: 

                2046     300      48
 
SfcI C'TryA_G

Cuts at:      0     316     544     796     954    1013    1097    1461    1641 
   Size:        316     228     252     158      59      84     364     180

Cuts at:   1641    1647    1836    1995    2394 
   Size:          6     189     159     399

  Fragments arranged by size: 

                 399     364     316     252     228     189     180     159
                 158      84      59       6
 
SfoI GGC'GCC

Cuts at:      0     342     371     742    2394 
   Size:        342      29     371    1652

  Fragments arranged by size: 

                1652     371     342      29
 
SmlI C'TyrA_G

Cuts at:      0     263    2394 
   Size:        263    2131
 
SphI G_CATG'C

Cuts at:      0    1787    2394 
   Size:       1787     607
 
StyI C'CwwG_G

Cuts at:      0    1377    1518    2033    2394 
   Size:       1377     141     515     361

  Fragments arranged by size: 

                1377     515     361     141
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     376     571    1351    1456    1565    2394 
   Size:        376     195     780     105     109     829

  Fragments arranged by size: 

                 829     780     376     195     109     105
 
TatI w'GTAC_w

Cuts at:      0     461    1999    2394 
   Size:        461    1538     395

  Fragments arranged by size: 

                1538     461     395
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     437     469    1561    1593    2343    2375    2394 
   Size:        437      32    1092      32     750      32      19

  Fragments arranged by size: 

                1092     750     437      32      32      32      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     820    1465    2025    2394 
   Size:        820     645     560     369

  Fragments arranged by size: 

                 820     645     560     369
 
XhoI C'TCGA_G

Cuts at:      0     263    2394 
   Size:        263    2131
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FspI    FspAI    HaeII    Hin4I     KasI     MluI     MscI
     MslI   MspA1I     NarI     NspI    PflMI    PpuMI     PsiI   PspOMI
    PspXI     PstI    PvuII     SacI    SacII     SfcI     SfoI     SmlI
     SphI     StyI    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BclI
    BfrBI     BglI    BglII     BmtI    BpuEI    BsaAI    BsaBI    BsaWI
    BsaXI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI    EcoNI    EcoRI    EcoRV     FalI     FseI   HincII
  HindIII     HpaI     KpnI     MfeI     MmeI     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsrI     PvuI    RsrII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XmaI     XmnI     ZraI
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