Restriction map for pF1KB2389
 (Linear) (Six-base) MAPSORT of: KIBB2389.seq  Check: 9475  from: 1  to: 3174



KIBB2389.orf

 With 182 enzymes: SgfI * 

                        August 12, 2008 14:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     974    1110    1422    3037    3174 
   Size:        974     136     312    1615     137

  Fragments arranged by size: 

                1615     974     312     137     136
 
AatII G_ACGT'C

Cuts at:      0     488    1391    3174 
   Size:        488     903    1783

  Fragments arranged by size: 

                1783     903     488
 
AccI GT'mk_AC

Cuts at:      0    2000    3174 
   Size:       2000    1174
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1047    1201    1975    2079    2106    2341    2725    3152 
   Size:       1047     154     774     104      27     235     384     427

Cuts at:   3152    3174 
   Size:         22

  Fragments arranged by size: 

                1047     774     427     384     235     154     104      27
                  22
 
AfeI AGC'GCT

Cuts at:      0     427    3097    3174 
   Size:        427    2670      77

  Fragments arranged by size: 

                2670     427      77
 
AflII C'TTAA_G

Cuts at:      0     147    3174 
   Size:        147    3027
 
AflIII A'CryG_T

Cuts at:      0     120     438     623    2042    3174 
   Size:        120     318     185    1419    1132

  Fragments arranged by size: 

                1419    1132     318     185     120
 
AleI CACnn'nnGTG

Cuts at:      0    2941    3174 
   Size:       2941     233
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2061    3174 
   Size:       2061    1113
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     271     303    3174 
   Size:        271      32    2871

  Fragments arranged by size: 

                2871     271      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     384     864    1927    2910    3174 
   Size:        384     480    1063     983     264

  Fragments arranged by size: 

                1063     983     480     384     264
 
ApaI G_GGCC'C

Cuts at:      0     697    1338    3174 
   Size:        697     641    1836

  Fragments arranged by size: 

                1836     697     641
 
ApaLI G'TGCA_C

Cuts at:      0    1663    3174 
   Size:       1663    1511
 
ApoI r'AATT_y

Cuts at:      0    1960    3174 
   Size:       1960    1214
 
AvaI C'yCGr_G

Cuts at:      0     696    3174 
   Size:        696    2478
 
AvrII C'CTAG_G

Cuts at:      0       6    3174 
   Size:          6    3168
 
BamHI G'GATC_C

Cuts at:      0     686    3174 
   Size:        686    2488
 
BanI G'GyrC_C

Cuts at:      0    1717    2156    3140    3174 
   Size:       1717     439     984      34

  Fragments arranged by size: 

                1717     984     439      34
 
BanII G_rGCy'C

Cuts at:      0     697    1338    1880    2204    2444    3174 
   Size:        697     641     542     324     240     730

  Fragments arranged by size: 

                 730     697     641     542     324     240
 
BbeI G_GCGC'C

Cuts at:      0    3144    3174 
   Size:       3144      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     287     470    1577    2399    3174 
   Size:        287     183    1107     822     775

  Fragments arranged by size: 

                1107     822     775     287     183
 
BbvCI CC'TCA_GC

Cuts at:      0    3116    3174 
   Size:       3116      58
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1158    1192    3174 
   Size:       1158      34    1982

  Fragments arranged by size: 

                1982    1158      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1513    3174 
   Size:       1513    1661
 
BclI T'GATC_A

Cuts at:      0     209    3174 
   Size:        209    2965
 
BglI GCCn_nnn'nGGC

Cuts at:      0     727    1645    3174 
   Size:        727     918    1529

  Fragments arranged by size: 

                1529     918     727
 
BglII A'GATC_T

Cuts at:      0     155    2375    3174 
   Size:        155    2220     799

  Fragments arranged by size: 

                2220     799     155
 
Bme1580I G_kGCm'C

Cuts at:      0     631     697    1338    1667    2342    3087    3174 
   Size:        631      66     641     329     675     745      87

  Fragments arranged by size: 

                 745     675     641     631     329      87      66
 
BmgBI CAC'GTC

Cuts at:      0     468    2517    3174 
   Size:        468    2049     657

  Fragments arranged by size: 

                2049     657     468
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     123     534     920    3174 
   Size:        123     411     386    2254

  Fragments arranged by size: 

                2254     411     386     123
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     177    2256    2421    3174 
   Size:        177    2079     165     753

  Fragments arranged by size: 

                2079     753     177     165
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2050    2218    2457    2659    3061    3174 
   Size:       2050     168     239     202     402     113

  Fragments arranged by size: 

                2050     402     239     202     168     113
 
Bpu10I CC'TnA_GC

Cuts at:      0     607    3116    3174 
   Size:        607    2509      58

  Fragments arranged by size: 

                2509     607      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1957    1998    3174 
   Size:       1957      41    1176

  Fragments arranged by size: 

                1957    1176      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     925    1131    2227    3174 
   Size:        925     206    1096     947

  Fragments arranged by size: 

                1096     947     925     206
 
BsaAI yAC'GTr

Cuts at:      0    2814    3174 
   Size:       2814     360
 
BsaBI GATnn'nnATC

Cuts at:      0     214    2020    2191    3174 
   Size:        214    1806     171     983

  Fragments arranged by size: 

                1806     983     214     171
 
BsaHI Gr'CG_yC

Cuts at:      0     485    1388    3141    3174 
   Size:        485     903    1753      33

  Fragments arranged by size: 

                1753     903     485      33
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2140    2170    2191    2221    3174 
   Size:       2140      30      21      30     953

  Fragments arranged by size: 

                2140     953      30      30      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     150    1000    2080    2545    2641    2815    3174 
   Size:        150     850    1080     465      96     174     359

  Fragments arranged by size: 

                1080     850     465     359     174     150      96
 
BseYI C'CCAG_C

Cuts at:      0     179    3174 
   Size:        179    2995
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     943    1713    1798    2610    2944    3174 
   Size:        943     770      85     812     334     230

  Fragments arranged by size: 

                 943     812     770     334     230      85
 
BsiHKAI G_wGCw'C

Cuts at:      0    1004    1174    1667    1880    2204    3174 
   Size:       1004     170     493     213     324     970

  Fragments arranged by size: 

                1004     970     493     324     213     170
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2475    3174 
   Size:       2475     699
 
Bsp1286I G_dGCh'C

Cuts at:      0     631     697    1004    1174    1338    1667    1880    2204 
   Size:        631      66     307     170     164     329     213     324

Cuts at:   2204    2342    2444    3087    3174 
   Size:        138     102     643      87

  Fragments arranged by size: 

                 643     631     329     324     307     213     170     164
                 138     102      87      66
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     575     974    1110    1422    1489    2080    3037    3174 
   Size:        575     399     136     312      67     591     957     137

  Fragments arranged by size: 

                 957     591     575     399     312     137     136      67
            
 
BsrDI GCAATG_nn'

Cuts at:      0     557    1214    3110    3174 
   Size:        557     657    1896      64

  Fragments arranged by size: 

                1896     657     557      64
 
BsrFI r'CCGG_y

Cuts at:      0    1037    3174 
   Size:       1037    2137
 
BssSI C'ACGA_G

Cuts at:      0    3088    3174 
   Size:       3088      86
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     966     999    2580    3174 
   Size:        966      33    1581     594

  Fragments arranged by size: 

                1581     966     594      33
 
BstEII G'GTnAC_C

Cuts at:      0     514    3174 
   Size:        514    2660
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     942    1221    1806    3174 
   Size:        942     279     585    1368

  Fragments arranged by size: 

                1368     942     585     279
 
BstYI r'GATC_y

Cuts at:      0     155     674     686    1424    2015    2178    2375    3174 
   Size:        155     519      12     738     591     163     197     799

  Fragments arranged by size: 

                 799     738     591     519     197     163     155      12
            
 
Bsu36I CC'TnA_GG

Cuts at:      0      83    1159    1762    2314    2528    2597    2746    3174 
   Size:         83    1076     603     552     214      69     149     428

  Fragments arranged by size: 

                1076     603     552     428     214     149      83      69
            
 
BtgI C'CryG_G

Cuts at:      0     660     809    1220    1643    3174 
   Size:        660     149     411     423    1531

  Fragments arranged by size: 

                1531     660     423     411     149
 
BtsI GCAGTG_nn'

Cuts at:      0    1745    3174 
   Size:       1745    1429
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1616    1651    2352    2387    3174 
   Size:       1616      35     701      35     787

  Fragments arranged by size: 

                1616     787     701      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     246    3174 
   Size:        246    2928
 
EaeI y'GGCC_r

Cuts at:      0      62     647     719     806     914    1223    2969    3174 
   Size:         62     585      72      87     108     309    1746     205

  Fragments arranged by size: 

                1746     585     309     205     108      87      72      62
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     340    1345    1937    1988    2098    2317    2746    3174 
   Size:        340    1005     592      51     110     219     429     428

  Fragments arranged by size: 

                1005     592     429     428     340     219     110      51
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1153    3174 
   Size:       1153    2021
 
EcoICRI GAG'CTC

Cuts at:      0    1878    2202    3174 
   Size:       1878     324     972

  Fragments arranged by size: 

                1878     972     324
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1047    1201    1975    2050    2079    2106    2218    2341 
   Size:       1047     154     774      75      29      27     112     123

Cuts at:   2341    2457    2659    2725    3061    3152    3174 
   Size:        116     202      66     336      91      22

  Fragments arranged by size: 

                1047     774     336     202     154     123     116     112
                  91      75      66      29      27      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1105    2798    2914    3174 
   Size:       1105    1693     116     260

  Fragments arranged by size: 

                1693    1105     260     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10     525    1190    1334    1335    1527    1783    2907 
   Size:         10     515     665     144       1     192     256    1124

Cuts at:   2907    3174 
   Size:        267

  Fragments arranged by size: 

                1124     665     515     267     256     192     144      10
                   1
 
EcoRV GAT'ATC

Cuts at:      0    1203    3174 
   Size:       1203    1971
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1311    2346    3174 
   Size:       1311    1035     828

  Fragments arranged by size: 

                1311    1035     828
 
HaeII r_GCGC'y

Cuts at:      0     429     865    2613    3099    3144    3174 
   Size:        429     436    1748     486      45      30

  Fragments arranged by size: 

                1748     486     436     429      45      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     119     151     359     391     666     698    2203    2235 
   Size:        119      32     208      32     275      32    1505      32

Cuts at:   2235    2484    2516    3174 
   Size:        249      32     658

  Fragments arranged by size: 

                1505     658     275     249     208     119      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     450    3174 
   Size:        450    2724
 
HindIII A'AGCT_T

Cuts at:      0     359    2383    3174 
   Size:        359    2024     791

  Fragments arranged by size: 

                2024     791     359
 
KasI G'GCGC_C

Cuts at:      0    3140    3174 
   Size:       3140      34
 
MluI A'CGCG_T

Cuts at:      0     623    2042    3174 
   Size:        623    1419    1132

  Fragments arranged by size: 

                1419    1132     623
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     281     664    3174 
   Size:        105     176     383    2510

  Fragments arranged by size: 

                2510     383     176     105
 
MscI TGG'CCA

Cuts at:      0     649     721     808     916    2971    3174 
   Size:        649      72      87     108    2055     203

  Fragments arranged by size: 

                2055     649     203     108      87      72
 
MslI CAynn'nnrTG

Cuts at:      0     940    1219    1804    2941    2971    3174 
   Size:        940     279     585    1137      30     203

  Fragments arranged by size: 

                1137     940     585     279     203      30
 
MspA1I CmG'CkG

Cuts at:      0      72     662    1399    2477    2622    2878    3174 
   Size:         72     590     737    1078     145     256     296

  Fragments arranged by size: 

                1078     737     590     296     256     145      72
 
NarI GG'CG_CC

Cuts at:      0    3141    3174 
   Size:       3141      33
 
NcoI C'CATG_G

Cuts at:      0    1220    3174 
   Size:       1220    1954
 
NdeI CA'TA_TG

Cuts at:      0    1253    3174 
   Size:       1253    1921
 
NspI r_CATG'y

Cuts at:      0     442     738    3174 
   Size:        442     296    2436

  Fragments arranged by size: 

                2436     442     296
 
PasI CC'CwG_GG

Cuts at:      0    2419    3174 
   Size:       2419     755
 
PciI A'CATG_T

Cuts at:      0     438    3174 
   Size:        438    2736
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2112    2196    3174 
   Size:       2112      84     978

  Fragments arranged by size: 

                2112     978      84
 
PfoI T'CCnGG_A

Cuts at:      0     479    1448    2195    2849    3174 
   Size:        479     969     747     654     325

  Fragments arranged by size: 

                 969     747     654     479     325
 
PmlI CAC'GTG

Cuts at:      0    2814    3174 
   Size:       2814     360
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90    3174 
   Size:         58      32    3084

  Fragments arranged by size: 

                3084      58      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      10     525    1783    2907    3174 
   Size:         10     515    1258    1124     267

  Fragments arranged by size: 

                1258    1124     515     267      10
 
PshAI GACnn'nnGTC

Cuts at:      0    2006    3174 
   Size:       2006    1168
 
PspOMI G'GGCC_C

Cuts at:      0     693    1334    3174 
   Size:        693     641    1840

  Fragments arranged by size: 

                1840     693     641
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1494    1526    3174 
   Size:       1494      32    1648

  Fragments arranged by size: 

                1648    1494      32
 
PstI C_TGCA'G

Cuts at:      0     171     236    2770    3174 
   Size:        171      65    2534     404

  Fragments arranged by size: 

                2534     404     171      65
 
PvuII CAG'CTG

Cuts at:      0      72    1399    2622    2878    3174 
   Size:         72    1327    1223     256     296

  Fragments arranged by size: 

                1327    1223     296     256      72
 
SacI G_AGCT'C

Cuts at:      0    1880    2204    3174 
   Size:       1880     324     970

  Fragments arranged by size: 

                1880     970     324
 
SacII CC_GC'GG

Cuts at:      0     663    3174 
   Size:        663    2511
 
SanDI GG'GwC_CC

Cuts at:      0    1783    3174 
   Size:       1783    1391
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1937    2317    3174 
   Size:       1937     380     857

  Fragments arranged by size: 

                1937     857     380
 
SfcI C'TryA_G

Cuts at:      0     167     232    2766    3174 
   Size:        167      65    2534     408

  Fragments arranged by size: 

                2534     408     167      65
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1645    3174 
   Size:       1645    1529
 
SfoI GGC'GCC

Cuts at:      0    3142    3174 
   Size:       3142      32
 
SmaI CCC'GGG

Cuts at:      0     698    3174 
   Size:        698    2476
 
SmlI C'TyrA_G

Cuts at:      0     147    1936    1977    3174 
   Size:        147    1789      41    1197

  Fragments arranged by size: 

                1789    1197     147      41
 
StuI AGG'CCT

Cuts at:      0     145    3174 
   Size:        145    3029
 
StyI C'CwwG_G

Cuts at:      0       6     224    1106    1193    1220    1853    1911    2144 
   Size:          6     218     882      87      27     633      58     233

Cuts at:   2144    2584    2884    3014    3174 
   Size:        440     300     130     160

  Fragments arranged by size: 

                 882     633     440     300     233     218     160     130
                  87      58      27       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     853    1735    1866    2432    2953    3174 
   Size:        853     882     131     566     521     221

  Fragments arranged by size: 

                 882     853     566     521     221     131
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     475     507     696     728     828     860    2140    2172 
   Size:        475      32     189      32     100      32    1280      32

Cuts at:   2172    2191    2223    3174 
   Size:         19      32     951

  Fragments arranged by size: 

                1280     951     475     189     100      32      32      32
                  32      32      19
 
Tth111I GACn'n_nGTC

Cuts at:      0     252    2857    3174 
   Size:        252    2605     317

  Fragments arranged by size: 

                2605     317     252
 
XbaI T'CTAG_A

Cuts at:      0    2031    3174 
   Size:       2031    1143
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     642    2634    3174 
   Size:        642    1992     540

  Fragments arranged by size: 

                1992     642     540
 
XmaI C'CCGG_G

Cuts at:      0     696    3174 
   Size:        696    2478
 
XmnI GAAnn'nnTTC

Cuts at:      0    2326    2678    3174 
   Size:       2326     352     496

  Fragments arranged by size: 

                2326     496     352
 
ZraI GAC'GTC

Cuts at:      0     486    1389    3174 
   Size:        486     903    1785

  Fragments arranged by size: 

                1785     903     486
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI    AflII   AflIII     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI
    CspCI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     KasI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PsrI     PstI    PvuII     SacI    SacII    SanDI
     SapI     SfcI     SfiI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AgeI     AhdI     AscI     AseI    AsiSI     BaeI
    BfrBI     BlpI     BmtI    BsaWI    BsiEI    BsiWI     BsmI    BspEI
    BspHI    BsrBI    BsrGI   BssHII    BstBI  BstZ17I    BtgZI     ClaI
     DraI     DrdI     EagI    EcoRI     FseI     FspI    FspAI     HpaI
     KpnI     MfeI     NaeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PmeI     PsiI    PspXI     PvuI    RsrII     SalI     SbfI
     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI     XhoI
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