Restriction map for pF1KB1630
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       AscI        
AspEI       AspI        AviII       BalI        BamHI       
BbrPI       BcgI        BclI        BlpI        Bpu1102I    
BsaAI       BsaI        BsaWI       BseAI       BsgI        
BsiWI       Bsp1407I    BspEI       BspHI       BsrBI       
BsrFI       BsrGI       BstAPI      BstEII      BstPI       
BstXI       Bsu36I      CelII       Cfr10I      CpoI        
DraI        DraII       DrdI        Eam1105I    Eco81I      
EcoNI       EcoO109I    EcoO65I     FbaI        FseI        
FspI        HincII      HindII      HpaI        MluNI       
MroI        MscI        NaeI        NcoI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    RcaI        
RsrII       SalI        SbfI        SfiI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AflII        2965  CTTAAG               C^TTAAG
Aor51HI       148  AGCGCT               AGC^GCT
AseI         1281  ATTAAT               AT^TAAT
AsnI         1281  ATTAAT               AT^TAAT
AvaI           37  CCCGAG               C^[CT]CG[GA]G
AvrII         624  CCTAGG               C^CTAGG
BbeI          368  GGCGCC               GGCGC^C
BbvCI        1245  CCTCAGC              CCTCAGC(-5/-2)
BfrI         2965  CTTAAG               C^TTAAG
BglI          107  GCCCCGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          624  CCTAGG               C^CTAGG
BsiYI          74  CCAGCGGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           74  CCAGCGGCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI         815  GAGACG               CGTCTC(1/5)
BsmFI         155  GTCCC                GGGAC(10/14)
BsmI         2176  GCATTC               GAATGC(1/-1)
BsoBI          37  CCCGAG               C^[CT]CG[GA]G
BspDI        2020  ATCGAT               AT^CGAT
BspLU11I     1977  ACATGT               A^CATGT
BsrDI        2980  GCAATG               GCAATG(2/0)
BssHII         44  GCGCGC               G^CGCGC
BssSI        1499  CACGAG               CACGAG(-5/-1)
Bst1107I     1269  GTATAC               GTA^TAC
BstBI        2140  TTCGAA               TT^CGAA
BstZ17I      1269  GTATAC               GTA^TAC
ClaI         2020  ATCGAT               AT^CGAT
DraIII       2181  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI            8  CCGCGG               C^C[GA][CT]GG
EagI           11  CGGCCG               C^GGCCG
EclXI          11  CGGCCG               C^GGCCG
Eco47III      148  AGCGCT               AGC^GCT
Eco52I         11  CGGCCG               C^GGCCG
EcoRV        2017  GATATC               GAT^ATC
HindIII      2506  AAGCTT               A^AGCTT
KasI          368  GGCGCC               G^GCGCC
KspI            8  CCGCGG               CCGC^GG
MfeI         2529  CAATTG               C^AATTG
MluI         1675  ACGCGT               A^CGCGT
MslI         1344  CATCCTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI         2529  CAATTG               C^AATTG
NarI          368  GGCGCC               GG^CGCC
NspV         2140  TTCGAA               TT^CGAA
PshBI        1281  ATTAAT               AT^TAAT
PvuI           68  CGATCG               CGAT^CG
SacII           8  CCGCGG               CCGC^GG
SexAI        1401  ACCTGGT              A^CC[AT]GGT
SfoI          368  GGCGCC               GGC^GCC
SfuI         2140  TTCGAA               TT^CGAA
SmlI         2965  CTTAAG               C^T[CT][GA]AG
SpeI         1137  ACTAGT               A^CTAGT
XcmI         2972  CCAGAACTGCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        753  GGTACC               G^GTACC
             2397  GGTACC              
AccI          714  GTCTAC               GT^[AC][GT]AC
             1269  GTATAC              
AflIII       1675  ACGCGT               A^C[GA][CT]GT
             1977  ACATGT              
AlwNI        1397  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1918  CAGAATCTG           
Asp700I      1945  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2248  GAAGCTCTTC          
Asp718I       753  GGTACC               G^GTACC
             2397  GGTACC              
BanII        2677  GAGCTC               G[GA]GC[CT]^C
             2986  GAGCTC              
BciVI        2115  GGATAC               GTATCC(6/5)
              602  GTATCC              
BcnI          261  CCGGG                CC^[GC]GG
              829  CCGGG               
BglII         766  AGATCT               A^GATCT
             1755  AGATCT              
BsiEI          11  CGGCCG               CG[GA][CT]^CG
               68  CGATCG              
BsmAI         815  GAGAC                GTCTC(1/5)
             1611  GAGAC               
BspMI         321  ACCTGC               ACCTGC(4/8)
             1580  GCAGGT              
EaeI           11  CGGCCG               [CT]^GGCC[GA]
              143  CGGCCA              
HaeII         148  AGCGCT               [GA]GCGC^[CT]
              368  GGCGCC              
HapII         261  CCGG                 C^CGG
              829  CCGG                
HpaII         261  CCGG                 C^CGG
              829  CCGG                
HphI          196  TCACC                GGTGA(8/7)
              346  TCACC               
KpnI          753  GGTACC               GGTAC^C
             2397  GGTACC              
MspI          261  CCGG                 C^CGG
              829  CCGG                
NciI          261  CCGGG                CC^[GC]GG
              829  CCGGG               
NspI         1556  GCATGT               [GA]CATG^[CT]
             1977  ACATGT              
PstI         1578  CTGCAG               CTGCA^G
             2054  CTGCAG              
PvuII        2651  CAGCTG               CAG^CTG
             2800  CAGCTG              
SacI         2677  GAGCTC               GAGCT^C
             2986  GAGCTC              
SapI          454  GAAGAGC              GCTCTTC(1/4)
             2251  GCTCTTC             
ScaI         1503  AGTACT               AGT^ACT
             1574  AGTACT              
SspI          180  AATATT               AAT^ATT
             2645  AATATT              
Tsp45I        195  GTCAC                ^GT[GC]AC
             1044  GTCAC               
XmnI         1945  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2248  GAAGCTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        ApoI        AspHI       AvaII       
BanI        BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaBI       BsaHI       BsaJI       
BseRI       BsiHKAI     Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRI       EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeIII      HgaI        HhaI        
Hin1I       HinP1I      HinfI       ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MvaI        MvnI        MwoI        NdeI        NdeII       
NlaIII      NlaIV       NsiI        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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