Restriction map for pF1KB1380
 (Linear) (Six-base) MAPSORT of: KIBB1380.seq  Check: 1517  from: 1  to: 2121



KIBB1380.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     453     705    1125    1797    2121 
   Size:        105     348     252     420     672     324

  Fragments arranged by size: 

                 672     420     348     324     252     105
 
AflIII A'CryG_T

Cuts at:      0     529     620     958    2121 
   Size:        529      91     338    1163

  Fragments arranged by size: 

                1163     529     338      91
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1680    2121 
   Size:       1680     441
 
ApaI G_GGCC'C

Cuts at:      0    1404    2121 
   Size:       1404     717
 
ApaLI G'TGCA_C

Cuts at:      0    1765    2121 
   Size:       1765     356
 
ApoI r'AATT_y

Cuts at:      0     228     634    1233    1485    1737    1905    2121 
   Size:        228     406     599     252     252     168     216

  Fragments arranged by size: 

                 599     406     252     252     228     216     168
 
BanII G_rGCy'C

Cuts at:      0    1404    2121 
   Size:       1404     717
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      39     211    2121 
   Size:         39     172    1910

  Fragments arranged by size: 

                1910     172      39
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     156    2121 
   Size:        156    1965
 
BfrBI ATG'CAT

Cuts at:      0    2007    2121 
   Size:       2007     114
 
BglII A'GATC_T

Cuts at:      0    1071    2121 
   Size:       1071    1050
 
Bme1580I G_kGCm'C

Cuts at:      0    1404    1769    2121 
   Size:       1404     365     352

  Fragments arranged by size: 

                1404     365     352
 
BmgBI CAC'GTC

Cuts at:      0      36     621    2121 
   Size:         36     585    1500

  Fragments arranged by size: 

                1500     585      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1496    2121 
   Size:       1496     625
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     701    1541    1793    2121 
   Size:        133     568     840     252     328

  Fragments arranged by size: 

                 840     568     328     252     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326    2061    2121 
   Size:        326    1735      60

  Fragments arranged by size: 

                1735     326      60
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     344    1605    2121 
   Size:        344    1261     516

  Fragments arranged by size: 

                1261     516     344
 
BsaAI yAC'GTr

Cuts at:      0     530     959    2121 
   Size:        530     429    1162

  Fragments arranged by size: 

                1162     530     429
 
BsaBI GATnn'nnATC

Cuts at:      0     494    2006    2121 
   Size:        494    1512     115

  Fragments arranged by size: 

                1512     494     115
 
BsaHI Gr'CG_yC

Cuts at:      0    1848    2121 
   Size:       1848     273
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1054    2121 
   Size:       1054    1067
 
BseYI C'CCAG_C

Cuts at:      0      63     818    1649    2121 
   Size:         63     755     831     472

  Fragments arranged by size: 

                 831     755     472      63
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1666    2121 
   Size:       1666     455
 
BsiHKAI G_wGCw'C

Cuts at:      0    1769    2121 
   Size:       1769     352
 
BsmI GAATG_Cn'

Cuts at:      0     151     796     915    2121 
   Size:        151     645     119    1206

  Fragments arranged by size: 

                1206     645     151     119
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1839    2121 
   Size:       1839     282
 
Bsp1286I G_dGCh'C

Cuts at:      0    1404    1769    2121 
   Size:       1404     365     352

  Fragments arranged by size: 

                1404     365     352
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     137    2121 
   Size:        137    1984
 
BsrDI GCAATG_nn'

Cuts at:      0      26     456    2121 
   Size:         26     430    1665

  Fragments arranged by size: 

                1665     430      26
 
BsrGI T'GTAC_A

Cuts at:      0    1899    2121 
   Size:       1899     222
 
BssSI C'ACGA_G

Cuts at:      0    1152    1656    2121 
   Size:       1152     504     465

  Fragments arranged by size: 

                1152     504     465
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     451    2121 
   Size:        451    1670
 
BstYI r'GATC_y

Cuts at:      0     242    1071    1258    1323    2121 
   Size:        242     829     187      65     798

  Fragments arranged by size: 

                 829     798     242     187      65
 
Bsu36I CC'TnA_GG

Cuts at:      0     178    2121 
   Size:        178    1943
 
BtgI C'CryG_G

Cuts at:      0     920    2121 
   Size:        920    1201
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     985    2121 
   Size:        985    1136
 
EarI CTCTTCn'nnn_

Cuts at:      0      53    1129    1381    1454    1465    1633    1706    2121 
   Size:         53    1076     252      73      11     168      73     415

  Fragments arranged by size: 

                1076     415     252     168      73      73      53      11
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     133     453     701     705    1125    1541    1793 
   Size:        105      28     320     248       4     420     416     252

Cuts at:   1793    1797    2121 
   Size:          4     324

  Fragments arranged by size: 

                 420     416     324     320     252     248     105      28
                   4       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1401    2121 
   Size:       1401     720
 
EcoRI G'AATT_C

Cuts at:      0     228     634    1737    1905    2121 
   Size:        228     406    1103     168     216

  Fragments arranged by size: 

                1103     406     228     216     168
 
HaeII r_GCGC'y

Cuts at:      0    1992    2121 
   Size:       1992     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     227     259     636     668     960     992    1308    1340 
   Size:        227      32     377      32     292      32     316      32

Cuts at:   1340    1380    1412    2121 
   Size:         40      32     709

  Fragments arranged by size: 

                 709     377     316     292     227      40      32      32
                  32      32      32
 
MfeI C'AATT_G

Cuts at:      0    1620    2121 
   Size:       1620     501
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1301    2088    2121 
   Size:       1301     787      33

  Fragments arranged by size: 

                1301     787      33
 
MslI CAynn'nnrTG

Cuts at:      0     530     864     948    1032    1200    1284    1452    1536 
   Size:        530     334      84      84     168      84     168      84

Cuts at:   1536    1704    1788    1956    2040    2096    2121 
   Size:        168      84     168      84      56      25

  Fragments arranged by size: 

                 530     334     168     168     168     168      84      84
                  84      84      84      84      56      25
 
MspA1I CmG'CkG

Cuts at:      0    1817    2121 
   Size:       1817     304
 
NcoI C'CATG_G

Cuts at:      0     920    2121 
   Size:        920    1201
 
NdeI CA'TA_TG

Cuts at:      0    1088    2121 
   Size:       1088    1033
 
NsiI A_TGCA'T

Cuts at:      0    2009    2121 
   Size:       2009     112
 
PasI CC'CwG_GG

Cuts at:      0     444    2121 
   Size:        444    1677
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150     676     708    2121 
   Size:        118      32     526      32    1413

  Fragments arranged by size: 

                1413     526     118      32      32
 
PsiI TTA'TAA

Cuts at:      0     779    2121 
   Size:        779    1342
 
PspOMI G'GGCC_C

Cuts at:      0    1400    2121 
   Size:       1400     721
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     541     573    2121 
   Size:        541      32    1548

  Fragments arranged by size: 

                1548     541      32
 
PvuII CAG'CTG

Cuts at:      0    1817    2121 
   Size:       1817     304
 
SapI GCTCTTCn'nnn_

Cuts at:      0      53    1129    1381    2121 
   Size:         53    1076     252     740

  Fragments arranged by size: 

                1076     740     252      53
 
SexAI A'CCwGG_T

Cuts at:      0     334    2121 
   Size:        334    1787
 
SmlI C'TyrA_G

Cuts at:      0     341    2076    2121 
   Size:        341    1735      45

  Fragments arranged by size: 

                1735     341      45
 
StuI AGG'CCT

Cuts at:      0     802    2074    2121 
   Size:        802    1272      47

  Fragments arranged by size: 

                1272     802      47
 
StyI C'CwwG_G

Cuts at:      0     920    2121 
   Size:        920    1201
 
TatI w'GTAC_w

Cuts at:      0    1899    2121 
   Size:       1899     222
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1243    1275    2121 
   Size:       1243      32     846

  Fragments arranged by size: 

                1243     846      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     691     718     802     859     943    1138    1810    1909 
   Size:        691      27      84      57      84     195     672      99

Cuts at:   1909    1931    2121 
   Size:         22     190

  Fragments arranged by size: 

                 691     672     195     190      99      84      84      57
                  27      22
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApaI    ApaLI     ApoI    BanII     BbsI
    BciVI    BfrBI    BglII Bme1580I    BmgBI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BsrGI    BssSI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     EarI  Eco57MI EcoO109I    EcoRI
    HaeII    Hin4I     MfeI     MmeI     MslI   MspA1I     NcoI     NdeI
     NsiI     PasI     PpiI     PsiI   PspOMI     PsrI    PvuII     SapI
    SexAI     SmlI     StuI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI     BclI
     BglI     BlpI     BmrI     BmtI   Bpu10I    BsaWI    BsaXI    BsiEI
    BsiWI    BspEI    BspHI    BsrBI    BsrFI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MscI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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