Restriction map for
pF1KB0207
(Circular) (Six-base) MAPSORT of: pF1KB0207.seq Check: 6351 from: 1 to: 6182
pF1KB0207 6182 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2868 2868
Size: 6182
AatII G_ACGT'C
Cuts at: 3161 3161
Size: 6182
AccI GT'mk_AC
Cuts at: 3220 3220
Size: 6182
Acc65I G'GTAC_C
Cuts at: 3198 3198
Size: 6182
AclI AA'CG_TT
Cuts at: 6022 6022
Size: 6182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1322 2122 2953 4007 4439 4792 5454 1322
Size: 800 831 1054 432 353 662 2050
Fragments arranged by size:
2050 1054 831 800 662 432 353
AfeI AGC'GCT
Cuts at: 937 937
Size: 6182
AflIII A'CryG_T
Cuts at: 1562 3534 5334 5615 1562
Size: 1972 1800 281 2129
Fragments arranged by size:
2129 1972 1800 281
AleI CACnn'nnGTG
Cuts at: 1495 1495
Size: 6182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1355 2452 1355
Size: 1097 5085
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 760 792 760
Size: 32 6150
AlwNI CAG_nnn'CTG
Cuts at: 444 2646 3082 3487 4925 444
Size: 2202 436 405 1438 1701
Fragments arranged by size:
2202 1701 1438 436 405
ApaLI G'TGCA_C
Cuts at: 1420 5020 1420
Size: 3600 2582
ApoI r'AATT_y
Cuts at: 1781 3186 5535 5791 1781
Size: 1405 2349 256 2172
Fragments arranged by size:
2349 2172 1405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6182
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
AvaI C'yCGr_G
Cuts at: 1502 1546 2397 2704 2846 3202 1502
Size: 44 851 307 142 356 4482
Fragments arranged by size:
4482 851 356 307 142 44
BamHI G'GATC_C
Cuts at: 806 3207 806
Size: 2401 3781
BanI G'GyrC_C
Cuts at: 530 1414 2972 3198 3612 3860 3895 530
Size: 884 1558 226 414 248 35 2817
Fragments arranged by size:
2817 1558 884 414 248 226 35
BanII G_rGCy'C
Cuts at: 251 369 1138 1664 2070 3196 4226 251
Size: 118 769 526 406 1126 1030 2207
Fragments arranged by size:
2207 1126 1030 769 526 406 118
BbeI G_GCGC'C
Cuts at: 3616 3864 3616
Size: 248 5934
BbsI GAAGACnn'nnnn_
Cuts at: 654 2032 3112 3153 654
Size: 1378 1080 41 3683
Fragments arranged by size:
3683 1378 1080 41
BbvCI CC'TCA_GC
Cuts at: 1384 1384
Size: 6182
BciVI GTATCCnnnnn_n'
Cuts at: 4080 5136 6182 4080
Size: 1056 1046 4080
Fragments arranged by size:
4080 1056 1046
BclI T'GATC_A
Cuts at: 1022 3702 1022
Size: 2680 3502
BglII A'GATC_T
Cuts at: 1091 1799 1822 1838 3697 5734 1091
Size: 708 23 16 1859 2037 1539
Fragments arranged by size:
2037 1859 1539 708 23 16
BlpI GC'TnA_GC
Cuts at: 245 1730 2011 2125 2960 3294 245
Size: 1485 281 114 835 334 3133
Fragments arranged by size:
3133 1485 835 334 281 114
Bme1580I G_kGCm'C
Cuts at: 379 533 593 1424 2152 3807 3900 5024 379
Size: 154 60 831 728 1655 93 1124 1537
Fragments arranged by size:
1655 1537 1124 831 728 154 93 60
BmgBI CAC'GTC
Cuts at: 1497 1608 2139 1497
Size: 111 531 5540
Fragments arranged by size:
5540 531 111
BmrI ACTGGGnnnn_n'
Cuts at: 2073 2364 3394 3475 3810 5954 2073
Size: 291 1030 81 335 2144 2301
Fragments arranged by size:
2301 2144 1030 335 291 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4549 4549
Size: 6182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 811 1138 3071 757
Size: 54 327 1933 3868
Fragments arranged by size:
3868 1933 327 54
Bpu10I CC'TnA_GC
Cuts at: 403 1384 403
Size: 981 5201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1258 3358 4237 4951 5249 961
Size: 297 2100 879 714 298 1894
Fragments arranged by size:
2100 1894 879 714 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 817 1366 5886 817
Size: 549 4520 1113
Fragments arranged by size:
4520 1113 549
BsaAI yAC'GTr
Cuts at: 1020 1425 1530 1563 2628 3012 3535 1020
Size: 405 105 33 1065 384 523 3667
Fragments arranged by size:
3667 1065 523 405 384 105 33
BsaBI GATnn'nnATC
Cuts at: 6 572 3696 5733 6
Size: 566 3124 2037 455
Fragments arranged by size:
3124 2037 566 455
BsaHI Gr'CG_yC
Cuts at: 2637 3158 3613 3861 4563 6059 2637
Size: 521 455 248 702 1496 2760
Fragments arranged by size:
2760 1496 702 521 455 248
BsaWI w'CCGG_w
Cuts at: 1138 2051 2657 3371 3892 4570 4981 5128 5402
Size: 913 606 714 521 678 411 147 274
Cuts at: 5402 1138
Size: 1918
Fragments arranged by size:
1918 913 714 678 606 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 760 790 274
Size: 30 456 30 5666
Fragments arranged by size:
5666 456 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 573 868 916 1467 1602 1789 2995 298
Size: 275 295 48 551 135 187 1206 3485
Fragments arranged by size:
3485 1206 551 295 275 187 135 48
BseYI C'CCAG_C
Cuts at: 2283 2295 3075 3607 4365 5030 5438 2283
Size: 12 780 532 758 665 408 3027
Fragments arranged by size:
3027 780 758 665 532 408 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 517 2802 2962 215
Size: 302 2285 160 3435
Fragments arranged by size:
3435 2285 302 160
BsiEI CG_ry'CG
Cuts at: 104 1580 3069 3724 3770 5000 104
Size: 1476 1489 655 46 1230 1286
Fragments arranged by size:
1489 1476 1286 1230 655 46
BsiHKAI G_wGCw'C
Cuts at: 1424 1594 2177 2987 3196 3974 4164 5024 1424
Size: 170 583 810 209 778 190 860 2582
Fragments arranged by size:
2582 860 810 778 583 209 190 170
BsmI GAATG_Cn'
Cuts at: 363 1518 363
Size: 1155 5027
Bsp1286I G_dGCh'C
Cuts at: 251 369 379 533 593 1138 1424 1594 1664
Size: 118 10 154 60 545 286 170 70
Cuts at: 1664 2070 2152 2177 2987 3196 3807 3900 3974
Size: 406 82 25 810 209 611 93 74
Cuts at: 3974 4164 4226 5024 251
Size: 190 62 798 1409
Fragments arranged by size:
1409 810 798 611 545 406 286 209
190 170 154 118 93 82 74 70
62 60 25 10
BspEI T'CCGG_A
Cuts at: 1138 2657 1138
Size: 1519 4663
BspHI T'CATG_A
Cuts at: 4614 4614
Size: 6182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1400 2212 2868 3232 3446 3748 4129 1400
Size: 812 656 364 214 302 381 3453
Fragments arranged by size:
3453 812 656 381 364 302 214
BsrBI CCG'CTC
Cuts at: 50 1475 1680 2129 2145 2496 4474 4528 6012
Size: 1425 205 449 16 351 1978 54 1484
Cuts at: 6012 6177 50
Size: 165 55
Fragments arranged by size:
1978 1484 1425 449 351 205 165 55
54 16
BsrDI GCAATG_nn'
Cuts at: 4094 4094
Size: 6182
BsrFI r'CCGG_y
Cuts at: 1631 2821 3064 3094 4180 4361 1631
Size: 1190 243 30 1086 181 3452
Fragments arranged by size:
3452 1190 1086 243 181 30
BsrGI T'GTAC_A
Cuts at: 321 1685 321
Size: 1364 4818
BssSI C'ACGA_G
Cuts at: 475 2030 4453 5161 475
Size: 1555 2423 708 1496
Fragments arranged by size:
2423 1555 1496 708
BstAPI GCAn_nnn'nTGC
Cuts at: 238 1916 3559 238
Size: 1678 1643 2861
Fragments arranged by size:
2861 1678 1643
BstBI TT'CG_AA
Cuts at: 271 4543 271
Size: 4272 1910
BstEII G'GTnAC_C
Cuts at: 1540 1540
Size: 6182
BstXI CCAn_nnnn'nTGG
Cuts at: 760 1497 760
Size: 737 5445
BstYI r'GATC_y
Cuts at: 1 806 1091 1694 1799 1822 1838 2018 2642
Size: 805 285 603 105 23 16 180 624
Cuts at: 2642 2713 3207 3674 3697 4032 4278 4584 4596
Size: 71 494 467 23 335 246 306 12
Cuts at: 4596 4682 5734 1
Size: 86 1052 449
Fragments arranged by size:
1052 805 624 603 494 467 449 335
306 285 246 180 105 86 71 23
23 16 12
BtgI C'CryG_G
Cuts at: 1253 1925 2306 2448 1253
Size: 672 381 142 4987
Fragments arranged by size:
4987 672 381 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4132 4313 4476 4132
Size: 181 163 5838
Fragments arranged by size:
5838 181 163
BtsI GCAGTG_nn'
Cuts at: 377 500 802 3425 377
Size: 123 302 2623 3134
Fragments arranged by size:
3134 2623 302 123
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 879 914 2392 2427 879
Size: 35 1478 35 4634
Fragments arranged by size:
4634 1478 35 35
DraI TTT'AAA
Cuts at: 3181 3181
Size: 6182
DraIII CAC_nnn'GTG
Cuts at: 2172 2172
Size: 6182
DrdI GACnn_nn'nnGTC
Cuts at: 1612 2527 3157 3888 5232 1612
Size: 915 630 731 1344 2562
Fragments arranged by size:
2562 1344 915 731 630
EaeI y'GGCC_r
Cuts at: 872 1220 1353 1577 1641 2130 2309 2882 3066
Size: 348 133 224 64 489 179 573 184
Cuts at: 3066 3767 3941 4332 4359 5704 872
Size: 701 174 391 27 1345 1350
Fragments arranged by size:
1350 1345 701 573 489 391 348 224
184 179 174 133 64 27
EagI C'GGCC_G
Cuts at: 1577 3066 3767 1577
Size: 1489 701 3992
Fragments arranged by size:
3992 1489 701
EarI CTCTTCn'nnn_
Cuts at: 2818 4205 4415 2818
Size: 1387 210 4585
Fragments arranged by size:
4585 1387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2472 4390 5132 5278 5992 2472
Size: 1918 742 146 714 2662
Fragments arranged by size:
2662 1918 742 714 146
EcoICRI GAG'CTC
Cuts at: 3194 3194
Size: 6182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 811 1138 1322 2122 2953 3071 4007 4439
Size: 54 327 184 800 831 118 936 432
Cuts at: 4439 4792 5454 757
Size: 353 662 1485
Fragments arranged by size:
1485 936 831 800 662 432 353 327
184 118 54
EcoNI CCTnn'n_nnAGG
Cuts at: 655 2104 2932 655
Size: 1449 828 3905
Fragments arranged by size:
3905 1449 828
EcoO109I rG'GnC_Cy
Cuts at: 2238 3072 3321 2238
Size: 834 249 5099
Fragments arranged by size:
5099 834 249
EcoRI G'AATT_C
Cuts at: 3186 3186
Size: 6182
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4201 4201
Size: 6182
FspI TGC'GCA
Cuts at: 3963 3963
Size: 6182
HaeII r_GCGC'y
Cuts at: 939 3132 3616 3864 5094 5865 939
Size: 2193 484 248 1230 771 1256
Fragments arranged by size:
2193 1256 1230 771 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 565 589 597 621 714 746 1083 1115 1120
Size: 24 8 24 93 32 337 32 5
Cuts at: 1120 1152 1381 1413 2627 2659 2698 2730 2890
Size: 32 229 32 1214 32 39 32 160
Cuts at: 2890 2922 4024 4054 4056 4086 565
Size: 32 1102 30 2 30 2661
Fragments arranged by size:
2661 1214 1102 337 229 160 93 39
32 32 32 32 32 32 32 30
30 24 24 8 5 2
HincII GTy'rAC
Cuts at: 1434 1758 2598 3221 1434
Size: 324 840 623 4395
Fragments arranged by size:
4395 840 623 324
HindIII A'AGCT_T
Cuts at: 1811 1811
Size: 6182
KasI G'GCGC_C
Cuts at: 3612 3860 3612
Size: 248 5934
KpnI G_GTAC'C
Cuts at: 3202 3202
Size: 6182
MfeI C'AATT_G
Cuts at: 2583 2583
Size: 6182
MluI A'CGCG_T
Cuts at: 5615 5615
Size: 6182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4941 5125 4941
Size: 184 5998
MscI TGG'CCA
Cuts at: 874 1222 1355 2311 2884 3943 874
Size: 348 133 956 573 1059 3113
Fragments arranged by size:
3113 1059 956 573 348 133
MslI CAynn'nnrTG
Cuts at: 1030 1337 1495 1561 2212 4171 4298 1030
Size: 307 158 66 651 1959 127 2914
Fragments arranged by size:
2914 1959 651 307 158 127 66
MspA1I CmG'CkG
Cuts at: 546 595 915 1126 1246 2754 2904 3079 3293
Size: 49 320 211 120 1508 150 175 214
Cuts at: 3293 3607 3967 4751 4996 546
Size: 314 360 784 245 1732
Fragments arranged by size:
1732 1508 784 360 320 314 245 214
211 175 150 120 49
NaeI GCC'GGC
Cuts at: 3066 4363 3066
Size: 1297 4885
NarI GG'CG_CC
Cuts at: 3613 3861 3613
Size: 248 5934
NcoI C'CATG_G
Cuts at: 2306 2448 2306
Size: 142 6040
NgoMIV G'CCGG_C
Cuts at: 3064 4361 3064
Size: 1297 4885
NspI r_CATG'y
Cuts at: 1035 1167 1914 3235 3735 5338 1035
Size: 132 747 1321 500 1603 1879
Fragments arranged by size:
1879 1603 1321 747 500 132
PasI CC'CwG_GG
Cuts at: 555 829 555
Size: 274 5908
PciI A'CATG_T
Cuts at: 5334 5334
Size: 6182
PflMI CCAn_nnn'nTGG
Cuts at: 2139 2190 2454 2770 2139
Size: 51 264 316 5551
Fragments arranged by size:
5551 316 264 51
PfoI T'CCnGG_A
Cuts at: 2736 3134 2736
Size: 398 5784
PmeI GTTT'AAAC
Cuts at: 3181 3181
Size: 6182
PmlI CAC'GTG
Cuts at: 1425 1530 2628 1425
Size: 105 1098 4979
Fragments arranged by size:
4979 1098 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 760 792 1651 1683 4628 4660 760
Size: 32 859 32 2945 32 2282
Fragments arranged by size:
2945 2282 859 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3072 3072
Size: 6182
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2983 3015 2983
Size: 32 6150
PstI C_TGCA'G
Cuts at: 2781 3128 3229 3914 2781
Size: 347 101 685 5049
Fragments arranged by size:
5049 685 347 101
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6182
PvuII CAG'CTG
Cuts at: 546 1126 2904 3607 3967 546
Size: 580 1778 703 360 2761
Fragments arranged by size:
2761 1778 703 580 360
RsrII CG'GwC_CG
Cuts at: 4377 4377
Size: 6182
SacI G_AGCT'C
Cuts at: 3196 3196
Size: 6182
SalI G'TCGA_C
Cuts at: 3219 3219
Size: 6182
SanDI GG'GwC_CC
Cuts at: 3072 3072
Size: 6182
SapI GCTCTTCn'nnn_
Cuts at: 4205 4415 4205
Size: 210 5972
SbfI CC_TGCA'GG
Cuts at: 3229 3229
Size: 6182
ScaI AGT'ACT
Cuts at: 5699 5699
Size: 6182
SexAI A'CCwGG_T
Cuts at: 892 892
Size: 6182
SfcI C'TryA_G
Cuts at: 33 910 2777 3124 3225 3910 4878 5069 33
Size: 877 1867 347 101 685 968 191 1146
Fragments arranged by size:
1867 1146 968 877 685 347 191 101
SfoI GGC'GCC
Cuts at: 3614 3862 3614
Size: 248 5934
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
SmaI CCC'GGG
Cuts at: 2399 2848 3204 2399
Size: 449 356 5377
Fragments arranged by size:
5377 449 356
SmlI C'TyrA_G
Cuts at: 976 1237 3337 4252 4966 5228 976
Size: 261 2100 915 714 262 1930
Fragments arranged by size:
2100 1930 915 714 262 261
SpeI A'CTAG_T
Cuts at: 67 265 67
Size: 198 5984
SphI G_CATG'C
Cuts at: 1035 3235 3735 1035
Size: 2200 500 3482
Fragments arranged by size:
3482 2200 500
StyI C'CwwG_G
Cuts at: 1214 2306 2448 2994 3316 3670 1214
Size: 1092 142 546 322 354 3726
Fragments arranged by size:
3726 1092 546 354 322 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2403 3765 3898 4357 4566 2403
Size: 1362 133 459 209 4019
Fragments arranged by size:
4019 1362 459 209 133
TatI w'GTAC_w
Cuts at: 290 321 1685 2078 5697 290
Size: 31 1364 393 3619 775
Fragments arranged by size:
3619 1364 775 393 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1170 1202 5699 5731 1170
Size: 32 4497 32 1621
Fragments arranged by size:
4497 1621 32 32
Tth111I GACn'n_nGTC
Cuts at: 658 3979 658
Size: 3321 2861
XbaI T'CTAG_A
Cuts at: 945 3213 945
Size: 2268 3914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2961 3677 2961
Size: 716 5466
XmaI C'CCGG_G
Cuts at: 2397 2846 3202 2397
Size: 449 356 5377
Fragments arranged by size:
5377 449 356
ZraI GAC'GTC
Cuts at: 3159 3159
Size: 6182
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI ApaI AscI AvrII BaeI BcgI
BfrBI BglI BmtI BsiWI BsmBI BssHII BstZ17I Bsu36I
ClaI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspOMI PspXI SacII
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI