Restriction map for pF1KB0207
 (Linear) (Six-base) MAPSORT of: KIBB0207.seq  Check: 3825  from: 1  to: 3069



KIBB0207.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2760    3069 
   Size:       2760     309
 
AatII G_ACGT'C

Cuts at:      0    3053    3069 
   Size:       3053      16
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1214    2014    2845    3069 
   Size:       1214     800     831     224

  Fragments arranged by size: 

                1214     831     800     224
 
AfeI AGC'GCT

Cuts at:      0     829    3069 
   Size:        829    2240
 
AflIII A'CryG_T

Cuts at:      0    1454    3069 
   Size:       1454    1615
 
AleI CACnn'nnGTG

Cuts at:      0    1387    3069 
   Size:       1387    1682
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1247    2344    3069 
   Size:       1247    1097     725

  Fragments arranged by size: 

                1247    1097     725
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     652     684    3069 
   Size:        652      32    2385

  Fragments arranged by size: 

                2385     652      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     336    2538    2974    3069 
   Size:        336    2202     436      95

  Fragments arranged by size: 

                2202     436     336      95
 
ApaLI G'TGCA_C

Cuts at:      0    1312    3069 
   Size:       1312    1757
 
ApoI r'AATT_y

Cuts at:      0    1673    3069 
   Size:       1673    1396
 
AvaI C'yCGr_G

Cuts at:      0    1394    1438    2289    2596    2738    3069 
   Size:       1394      44     851     307     142     331

  Fragments arranged by size: 

                1394     851     331     307     142      44
 
BamHI G'GATC_C

Cuts at:      0     698    3069 
   Size:        698    2371
 
BanI G'GyrC_C

Cuts at:      0     422    1306    2864    3069 
   Size:        422     884    1558     205

  Fragments arranged by size: 

                1558     884     422     205
 
BanII G_rGCy'C

Cuts at:      0     143     261    1030    1556    1962    3069 
   Size:        143     118     769     526     406    1107

  Fragments arranged by size: 

                1107     769     526     406     143     118
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     546    1924    3004    3045    3069 
   Size:        546    1378    1080      41      24

  Fragments arranged by size: 

                1378    1080     546      41      24
 
BbvCI CC'TCA_GC

Cuts at:      0    1276    3069 
   Size:       1276    1793
 
BclI T'GATC_A

Cuts at:      0     914    3069 
   Size:        914    2155
 
BglII A'GATC_T

Cuts at:      0     983    1691    1714    1730    3069 
   Size:        983     708      23      16    1339

  Fragments arranged by size: 

                1339     983     708      23      16
 
BlpI GC'TnA_GC

Cuts at:      0     137    1622    1903    2017    2852    3069 
   Size:        137    1485     281     114     835     217

  Fragments arranged by size: 

                1485     835     281     217     137     114
 
Bme1580I G_kGCm'C

Cuts at:      0     271     425     485    1316    2044    3069 
   Size:        271     154      60     831     728    1025

  Fragments arranged by size: 

                1025     831     728     271     154      60
 
BmgBI CAC'GTC

Cuts at:      0    1389    1500    2031    3069 
   Size:       1389     111     531    1038

  Fragments arranged by size: 

                1389    1038     531     111
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1965    2256    3069 
   Size:       1965     291     813

  Fragments arranged by size: 

                1965     813     291
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     649     703    1030    2963    3069 
   Size:        649      54     327    1933     106

  Fragments arranged by size: 

                1933     649     327     106      54
 
Bpu10I CC'TnA_GC

Cuts at:      0     295    1276    3069 
   Size:        295     981    1793

  Fragments arranged by size: 

                1793     981     295
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     853    1150    3069 
   Size:        853     297    1919

  Fragments arranged by size: 

                1919     853     297
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     709    1258    3069 
   Size:        709     549    1811

  Fragments arranged by size: 

                1811     709     549
 
BsaAI yAC'GTr

Cuts at:      0     912    1317    1422    1455    2520    2904    3069 
   Size:        912     405     105      33    1065     384     165

  Fragments arranged by size: 

                1065     912     405     384     165     105      33
 
BsaBI GATnn'nnATC

Cuts at:      0     464    3069 
   Size:        464    2605
 
BsaHI Gr'CG_yC

Cuts at:      0    2529    3050    3069 
   Size:       2529     521      19

  Fragments arranged by size: 

                2529     521      19
 
BsaWI w'CCGG_w

Cuts at:      0    1030    1943    2549    3069 
   Size:       1030     913     606     520

  Fragments arranged by size: 

                1030     913     606     520
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     166     196     652     682    3069 
   Size:        166      30     456      30    2387

  Fragments arranged by size: 

                2387     456     166      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     190     465     760     808    1359    1494    1681    2887 
   Size:        190     275     295      48     551     135     187    1206

Cuts at:   2887    3069 
   Size:        182

  Fragments arranged by size: 

                1206     551     295     275     190     187     182     135
                  48
 
BseYI C'CCAG_C

Cuts at:      0    2175    2187    2967    3069 
   Size:       2175      12     780     102

  Fragments arranged by size: 

                2175     780     102      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     409    2694    2854    3069 
   Size:        107     302    2285     160     215

  Fragments arranged by size: 

                2285     302     215     160     107
 
BsiEI CG_ry'CG

Cuts at:      0    1472    2961    3069 
   Size:       1472    1489     108

  Fragments arranged by size: 

                1489    1472     108
 
BsiHKAI G_wGCw'C

Cuts at:      0    1316    1486    2069    2879    3069 
   Size:       1316     170     583     810     190

  Fragments arranged by size: 

                1316     810     583     190     170
 
BsmI GAATG_Cn'

Cuts at:      0     255    1410    3069 
   Size:        255    1155    1659

  Fragments arranged by size: 

                1659    1155     255
 
Bsp1286I G_dGCh'C

Cuts at:      0     143     261     271     425     485    1030    1316    1486 
   Size:        143     118      10     154      60     545     286     170

Cuts at:   1486    1556    1962    2044    2069    2879    3069 
   Size:         70     406      82      25     810     190

  Fragments arranged by size: 

                 810     545     406     286     190     170     154     143
                 118      82      70      60      25      10
 
BspEI T'CCGG_A

Cuts at:      0    1030    2549    3069 
   Size:       1030    1519     520

  Fragments arranged by size: 

                1519    1030     520
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1292    2104    2760    3069 
   Size:       1292     812     656     309

  Fragments arranged by size: 

                1292     812     656     309
 
BsrBI CCG'CTC

Cuts at:      0    1367    1572    2021    2037    2388    3069 
   Size:       1367     205     449      16     351     681

  Fragments arranged by size: 

                1367     681     449     351     205      16
 
BsrFI r'CCGG_y

Cuts at:      0    1523    2713    2956    2986    3069 
   Size:       1523    1190     243      30      83

  Fragments arranged by size: 

                1523    1190     243      83      30
 
BsrGI T'GTAC_A

Cuts at:      0     213    1577    3069 
   Size:        213    1364    1492

  Fragments arranged by size: 

                1492    1364     213
 
BssSI C'ACGA_G

Cuts at:      0     367    1922    3069 
   Size:        367    1555    1147

  Fragments arranged by size: 

                1555    1147     367
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     130    1808    3069 
   Size:        130    1678    1261

  Fragments arranged by size: 

                1678    1261     130
 
BstBI TT'CG_AA

Cuts at:      0     163    3069 
   Size:        163    2906
 
BstEII G'GTnAC_C

Cuts at:      0    1432    3069 
   Size:       1432    1637
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     652    1389    3069 
   Size:        652     737    1680

  Fragments arranged by size: 

                1680     737     652
 
BstYI r'GATC_y

Cuts at:      0     698     983    1586    1691    1714    1730    1910    2534 
   Size:        698     285     603     105      23      16     180     624

Cuts at:   2534    2605    3069 
   Size:         71     464

  Fragments arranged by size: 

                 698     624     603     464     285     180     105      71
                  23      16
 
BtgI C'CryG_G

Cuts at:      0    1145    1817    2198    2340    3069 
   Size:       1145     672     381     142     729

  Fragments arranged by size: 

                1145     729     672     381     142
 
BtsI GCAGTG_nn'

Cuts at:      0     269     392     694    3069 
   Size:        269     123     302    2375

  Fragments arranged by size: 

                2375     302     269     123
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     771     806    2284    2319    3069 
   Size:        771      35    1478      35     750

  Fragments arranged by size: 

                1478     771     750      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2064    3069 
   Size:       2064    1005
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1504    2419    3049    3069 
   Size:       1504     915     630      20

  Fragments arranged by size: 

                1504     915     630      20
 
EaeI y'GGCC_r

Cuts at:      0     764    1112    1245    1469    1533    2022    2201    2774 
   Size:        764     348     133     224      64     489     179     573

Cuts at:   2774    2958    3069 
   Size:        184     111

  Fragments arranged by size: 

                 764     573     489     348     224     184     179     133
                 111      64
 
EagI C'GGCC_G

Cuts at:      0    1469    2958    3069 
   Size:       1469    1489     111

  Fragments arranged by size: 

                1489    1469     111
 
EarI CTCTTCn'nnn_

Cuts at:      0    2710    3069 
   Size:       2710     359
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2364    3069 
   Size:       2364     705
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     649     703    1030    1214    2014    2845    2963    3069 
   Size:        649      54     327     184     800     831     118     106

  Fragments arranged by size: 

                 831     800     649     327     184     118     106      54
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     547    1996    2824    3069 
   Size:        547    1449     828     245

  Fragments arranged by size: 

                1449     828     547     245
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2130    2964    3069 
   Size:       2130     834     105

  Fragments arranged by size: 

                2130     834     105
 
HaeII r_GCGC'y

Cuts at:      0     831    3024    3069 
   Size:        831    2193      45

  Fragments arranged by size: 

                2193     831      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     457     481     489     513     606     638     975    1007 
   Size:        457      24       8      24      93      32     337      32

Cuts at:   1007    1012    1044    1273    1305    2519    2551    2590    2622 
   Size:          5      32     229      32    1214      32      39      32

Cuts at:   2622    2782    2814    3069 
   Size:        160      32     255

  Fragments arranged by size: 

                1214     457     337     255     229     160      93      39
                  32      32      32      32      32      32      32      24
                  24       8       5
 
HincII GTy'rAC

Cuts at:      0    1326    1650    2490    3069 
   Size:       1326     324     840     579

  Fragments arranged by size: 

                1326     840     579     324
 
HindIII A'AGCT_T

Cuts at:      0    1703    3069 
   Size:       1703    1366
 
MfeI C'AATT_G

Cuts at:      0    2475    3069 
   Size:       2475     594
 
MscI TGG'CCA

Cuts at:      0     766    1114    1247    2203    2776    3069 
   Size:        766     348     133     956     573     293

  Fragments arranged by size: 

                 956     766     573     348     293     133
 
MslI CAynn'nnrTG

Cuts at:      0     922    1229    1387    1453    2104    3069 
   Size:        922     307     158      66     651     965

  Fragments arranged by size: 

                 965     922     651     307     158      66
 
MspA1I CmG'CkG

Cuts at:      0     438     487     807    1018    1138    2646    2796    2971 
   Size:        438      49     320     211     120    1508     150     175

Cuts at:   2971    3069 
   Size:         98

  Fragments arranged by size: 

                1508     438     320     211     175     150     120      98
                  49
 
NaeI GCC'GGC

Cuts at:      0    2958    3069 
   Size:       2958     111
 
NcoI C'CATG_G

Cuts at:      0    2198    2340    3069 
   Size:       2198     142     729

  Fragments arranged by size: 

                2198     729     142
 
NgoMIV G'CCGG_C

Cuts at:      0    2956    3069 
   Size:       2956     113
 
NspI r_CATG'y

Cuts at:      0     927    1059    1806    3069 
   Size:        927     132     747    1263

  Fragments arranged by size: 

                1263     927     747     132
 
PasI CC'CwG_GG

Cuts at:      0     447     721    3069 
   Size:        447     274    2348

  Fragments arranged by size: 

                2348     447     274
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2031    2082    2346    2662    3069 
   Size:       2031      51     264     316     407

  Fragments arranged by size: 

                2031     407     316     264      51
 
PfoI T'CCnGG_A

Cuts at:      0    2628    3026    3069 
   Size:       2628     398      43

  Fragments arranged by size: 

                2628     398      43
 
PmlI CAC'GTG

Cuts at:      0    1317    1422    2520    3069 
   Size:       1317     105    1098     549

  Fragments arranged by size: 

                1317    1098     549     105
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     652     684    1543    1575    3069 
   Size:        652      32     859      32    1494

  Fragments arranged by size: 

                1494     859     652      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2964    3069 
   Size:       2964     105
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2875    2907    3069 
   Size:       2875      32     162

  Fragments arranged by size: 

                2875     162      32
 
PstI C_TGCA'G

Cuts at:      0    2673    3020    3069 
   Size:       2673     347      49

  Fragments arranged by size: 

                2673     347      49
 
PvuII CAG'CTG

Cuts at:      0     438    1018    2796    3069 
   Size:        438     580    1778     273

  Fragments arranged by size: 

                1778     580     438     273
 
SanDI GG'GwC_CC

Cuts at:      0    2964    3069 
   Size:       2964     105
 
SexAI A'CCwGG_T

Cuts at:      0     784    3069 
   Size:        784    2285
 
SfcI C'TryA_G

Cuts at:      0     802    2669    3016    3069 
   Size:        802    1867     347      53

  Fragments arranged by size: 

                1867     802     347      53
 
SmaI CCC'GGG

Cuts at:      0    2291    2740    3069 
   Size:       2291     449     329

  Fragments arranged by size: 

                2291     449     329
 
SmlI C'TyrA_G

Cuts at:      0     868    1129    3069 
   Size:        868     261    1940

  Fragments arranged by size: 

                1940     868     261
 
SpeI A'CTAG_T

Cuts at:      0     157    3069 
   Size:        157    2912
 
SphI G_CATG'C

Cuts at:      0     927    3069 
   Size:        927    2142
 
StyI C'CwwG_G

Cuts at:      0    1106    2198    2340    2886    3069 
   Size:       1106    1092     142     546     183

  Fragments arranged by size: 

                1106    1092     546     183     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2295    3069 
   Size:       2295     774
 
TatI w'GTAC_w

Cuts at:      0     182     213    1577    1970    3069 
   Size:        182      31    1364     393    1099

  Fragments arranged by size: 

                1364    1099     393     182      31
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1062    1094    3069 
   Size:       1062      32    1975

  Fragments arranged by size: 

                1975    1062      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     550    3069 
   Size:        550    2519
 
XbaI T'CTAG_A

Cuts at:      0     837    3069 
   Size:        837    2232
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2853    3069 
   Size:       2853     216
 
XmaI C'CCGG_G

Cuts at:      0    2289    2738    3069 
   Size:       2289     449     331

  Fragments arranged by size: 

                2289     449     331
 
ZraI GAC'GTC

Cuts at:      0    3051    3069 
   Size:       3051      18
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrBI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI     BtsI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
   HincII  HindIII     MfeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI
     PsrI     PstI    PvuII    SanDI    SexAI     SfcI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI    BfrBI
     BglI     BmtI     BplI    BsiWI    BsmBI    BspHI    BsrDI   BssHII
  BstZ17I   Bsu36I    BtgZI     ClaI     DraI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI
     MmeI     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     XmnI
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