Restriction map for pF1KB0150
 (Linear) (Six-base) MAPSORT of: KIBB0150.seq  Check: 3447  from: 1  to: 4392



KIBB0150.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AccI GT'mk_AC

Cuts at:      0    2029    4392 
   Size:       2029    2363
 
AclI AA'CG_TT

Cuts at:      0     404    4392 
   Size:        404    3988
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1594    1799    2989    3562    4392 
   Size:       1594     205    1190     573     830

  Fragments arranged by size: 

                1594    1190     830     573     205
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3135    4392 
   Size:       3135    1257
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2659    4392 
   Size:       2659    1733
 
AlwNI CAG_nnn'CTG

Cuts at:      0     271    1221    1932    2170    2323    3702    4392 
   Size:        271     950     711     238     153    1379     690

  Fragments arranged by size: 

                1379     950     711     690     271     238     153
 
ApoI r'AATT_y

Cuts at:      0     165     236     508    1269    2063    2591    2655    2790 
   Size:        165      71     272     761     794     528      64     135

Cuts at:   2790    3264    4392 
   Size:        474    1128

  Fragments arranged by size: 

                1128     794     761     528     474     272     165     135
                  71      64
 
AseI AT'TA_AT

Cuts at:      0    2341    3689    4392 
   Size:       2341    1348     703

  Fragments arranged by size: 

                2341    1348     703
 
AvaI C'yCGr_G

Cuts at:      0    4285    4392 
   Size:       4285     107
 
BanI G'GyrC_C

Cuts at:      0    2482    2862    3387    3681    4392 
   Size:       2482     380     525     294     711

  Fragments arranged by size: 

                2482     711     525     380     294
 
BanII G_rGCy'C

Cuts at:      0    1480    1775    1961    2209    2574    3234    3970    4392 
   Size:       1480     295     186     248     365     660     736     422

  Fragments arranged by size: 

                1480     736     660     422     365     295     248     186
            
 
BbeI G_GCGC'C

Cuts at:      0    2486    3391    4392 
   Size:       2486     905    1001

  Fragments arranged by size: 

                2486    1001     905
 
BbvCI CC'TCA_GC

Cuts at:      0    1618    2744    4392 
   Size:       1618    1126    1648

  Fragments arranged by size: 

                1648    1618    1126
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     319     353    4392 
   Size:        319      34    4039

  Fragments arranged by size: 

                4039     319      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     315     843    1980    4392 
   Size:        315     528    1137    2412

  Fragments arranged by size: 

                2412    1137     528     315
 
BclI T'GATC_A

Cuts at:      0    1947    4392 
   Size:       1947    2445
 
BfrBI ATG'CAT

Cuts at:      0    3412    3756    4392 
   Size:       3412     344     636

  Fragments arranged by size: 

                3412     636     344
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1216    2987    4042    4392 
   Size:       1216    1771    1055     350

  Fragments arranged by size: 

                1771    1216    1055     350
 
Bme1580I G_kGCm'C

Cuts at:      0    1631    2865    4392 
   Size:       1631    1234    1527

  Fragments arranged by size: 

                1631    1527    1234
 
BmgBI CAC'GTC

Cuts at:      0     842    4392 
   Size:        842    3550
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1505    1690    2641    3956    4392 
   Size:       1505     185     951    1315     436

  Fragments arranged by size: 

                1505    1315     951     436     185
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3881    4392 
   Size:       3881     511
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18    1502    1838    2873    3161    3298    3397    3686 
   Size:         18    1484     336    1035     288     137      99     289

Cuts at:   3686    4392 
   Size:        706

  Fragments arranged by size: 

                1484    1035     706     336     289     288     137      99
                  18
 
Bpu10I CC'TnA_GC

Cuts at:      0    1618    1968    2143    2744    4392 
   Size:       1618     350     175     601    1648

  Fragments arranged by size: 

                1648    1618     601     350     175
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379    2032    2317    3336    4392 
   Size:        379    1653     285    1019    1056

  Fragments arranged by size: 

                1653    1056    1019     379     285
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1641    4392 
   Size:       1641    2751
 
BsaHI Gr'CG_yC

Cuts at:      0    2483    3136    3388    4392 
   Size:       2483     653     252    1004

  Fragments arranged by size: 

                2483    1004     653     252
 
BsaWI w'CCGG_w

Cuts at:      0    1149    4392 
   Size:       1149    3243
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     929     959    1984    2014    4392 
   Size:        929      30    1025      30    2378

  Fragments arranged by size: 

                2378    1025     929      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     649    1361    2380    2434    3074    3295    4392 
   Size:        649     712    1019      54     640     221    1097

  Fragments arranged by size: 

                1097    1019     712     649     640     221      54
 
BseYI C'CCAG_C

Cuts at:      0      82     342     884    2949    2974    4032    4103    4392 
   Size:         82     260     542    2065      25    1058      71     289

  Fragments arranged by size: 

                2065    1058     542     289     260      82      71      25
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310    1028    1245    3565    4392 
   Size:        310     718     217    2320     827

  Fragments arranged by size: 

                2320     827     718     310     217
 
BsiEI CG_ry'CG

Cuts at:      0    1859    4392 
   Size:       1859    2533
 
BsiHKAI G_wGCw'C

Cuts at:      0    2605    3234    4392 
   Size:       2605     629    1158

  Fragments arranged by size: 

                2605    1158     629
 
BsmI GAATG_Cn'

Cuts at:      0     994    1539    2229    4392 
   Size:        994     545     690    2163

  Fragments arranged by size: 

                2163     994     690     545
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     848    4392 
   Size:        848    3544
 
Bsp1286I G_dGCh'C

Cuts at:      0    1480    1631    1775    1961    2209    2574    2605    2865 
   Size:       1480     151     144     186     248     365      31     260

Cuts at:   2865    3234    3970    4392 
   Size:        369     736     422

  Fragments arranged by size: 

                1480     736     422     369     365     260     248     186
                 151     144      31
 
BspEI T'CCGG_A

Cuts at:      0    1149    4392 
   Size:       1149    3243
 
BspHI T'CATG_A

Cuts at:      0     642     969    3017    4392 
   Size:        642     327    2048    1375

  Fragments arranged by size: 

                2048    1375     642     327
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     530    1307    1856    2627    4281    4392 
   Size:        530     777     549     771    1654     111

  Fragments arranged by size: 

                1654     777     771     549     530     111
 
BsrDI GCAATG_nn'

Cuts at:      0    2354    2674    3639    4392 
   Size:       2354     320     965     753

  Fragments arranged by size: 

                2354     965     753     320
 
BsrFI r'CCGG_y

Cuts at:      0    2199    2918    4392 
   Size:       2199     719    1474

  Fragments arranged by size: 

                2199    1474     719
 
BsrGI T'GTAC_A

Cuts at:      0    4148    4392 
   Size:       4148     244
 
BssSI C'ACGA_G

Cuts at:      0    2472    4392 
   Size:       2472    1920
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     271    4392 
   Size:        271    4121
 
BstEII G'GTnAC_C

Cuts at:      0    2386    4392 
   Size:       2386    2006
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4342    4392 
   Size:       4342      50
 
BstYI r'GATC_y

Cuts at:      0     550     822    1152    2319    3336    3456    4392 
   Size:        550     272     330    1167    1017     120     936

  Fragments arranged by size: 

                1167    1017     936     550     330     272     120
 
Bsu36I CC'TnA_GG

Cuts at:      0    1373    3297    3526    4392 
   Size:       1373    1924     229     866

  Fragments arranged by size: 

                1924    1373     866     229
 
BtgI C'CryG_G

Cuts at:      0    3103    3473    3533    4178    4277    4392 
   Size:       3103     370      60     645      99     115

  Fragments arranged by size: 

                3103     645     370     115      99      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1453    3568    4392 
   Size:       1453    2115     824

  Fragments arranged by size: 

                2115    1453     824
 
BtsI GCAGTG_nn'

Cuts at:      0     688    1233    1872    2406    2584    4392 
   Size:        688     545     639     534     178    1808

  Fragments arranged by size: 

                1808     688     639     545     534     178
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3809    3844    4176    4211    4392 
   Size:       3809      35     332      35     181

  Fragments arranged by size: 

                3809     332     181      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3006    4392 
   Size:       3006    1386
 
EaeI y'GGCC_r

Cuts at:      0    1856    2108    2920    2988    4392 
   Size:       1856     252     812      68    1404

  Fragments arranged by size: 

                1856    1404     812     252      68
 
EagI C'GGCC_G

Cuts at:      0    1856    4392 
   Size:       1856    2536
 
EarI CTCTTCn'nnn_

Cuts at:      0     449     626     756    1048    4392 
   Size:        449     177     130     292    3344

  Fragments arranged by size: 

                3344     449     292     177     130
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     591    4100    4392 
   Size:        591    3509     292

  Fragments arranged by size: 

                3509     591     292
 
EcoICRI GAG'CTC

Cuts at:      0    3232    4392 
   Size:       3232    1160
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      18    1502    1594    1799    1838    2873    2989    3161 
   Size:         18    1484      92     205      39    1035     116     172

Cuts at:   3161    3298    3397    3562    3686    4392 
   Size:        137      99     165     124     706

  Fragments arranged by size: 

                1484    1035     706     205     172     165     137     124
                 116      99      92      39      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1621    2146    2747    3935    4262    4392 
   Size:       1621     525     601    1188     327     130

  Fragments arranged by size: 

                1621    1188     601     525     327     130
 
EcoO109I rG'GnC_Cy

Cuts at:      0     346     363     891    1218    1614    3058    4044    4282 
   Size:        346      17     528     327     396    1444     986     238

Cuts at:   4282    4392 
   Size:        110

  Fragments arranged by size: 

                1444     986     528     396     346     327     238     110
                  17
 
FspI TGC'GCA

Cuts at:      0     926    4392 
   Size:        926    3466
 
HaeII r_GCGC'y

Cuts at:      0    2486    3391    4392 
   Size:       2486     905    1001

  Fragments arranged by size: 

                2486    1001     905
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     292     324     420     452     721     753     828     860 
   Size:        292      32      96      32     269      32      75      32

Cuts at:    860     951     983    2748    2780    3121    3153    3509    3541 
   Size:         91      32    1765      32     341      32     356      32

Cuts at:   3541    4392 
   Size:        851

  Fragments arranged by size: 

                1765     851     356     341     292     269      96      91
                  75      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2117    2502    4392 
   Size:       2117     385    1890

  Fragments arranged by size: 

                2117    1890     385
 
HindIII A'AGCT_T

Cuts at:      0    1533    1734    4392 
   Size:       1533     201    2658

  Fragments arranged by size: 

                2658    1533     201
 
HpaI GTT'AAC

Cuts at:      0    2117    4392 
   Size:       2117    2275
 
KasI G'GCGC_C

Cuts at:      0    2482    3387    4392 
   Size:       2482     905    1005

  Fragments arranged by size: 

                2482    1005     905
 
MfeI C'AATT_G

Cuts at:      0     594    4392 
   Size:        594    3798
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     580    1964    2547    3389    4076    4392 
   Size:        580    1384     583     842     687     316

  Fragments arranged by size: 

                1384     842     687     583     580     316
 
MscI TGG'CCA

Cuts at:      0    2110    2990    4392 
   Size:       2110     880    1402

  Fragments arranged by size: 

                2110    1402     880
 
MslI CAynn'nnrTG

Cuts at:      0     968    1450    2812    2965    4144    4171    4392 
   Size:        968     482    1362     153    1179      27     221

  Fragments arranged by size: 

                1362    1179     968     482     221     153      27
 
MspA1I CmG'CkG

Cuts at:      0     271    1808    1929    2542    3208    3246    3483    4338 
   Size:        271    1537     121     613     666      38     237     855

Cuts at:   4338    4392 
   Size:         54

  Fragments arranged by size: 

                1537     855     666     613     271     237     121      54
                  38
 
NarI GG'CG_CC

Cuts at:      0    2483    3388    4392 
   Size:       2483     905    1004

  Fragments arranged by size: 

                2483    1004     905
 
NcoI C'CATG_G

Cuts at:      0    3103    3473    4178    4277    4392 
   Size:       3103     370     705      99     115

  Fragments arranged by size: 

                3103     705     370     115      99
 
NdeI CA'TA_TG

Cuts at:      0    1112    4392 
   Size:       1112    3280
 
NsiI A_TGCA'T

Cuts at:      0    3414    3758    4392 
   Size:       3414     344     634

  Fragments arranged by size: 

                3414     634     344
 
NspI r_CATG'y

Cuts at:      0     493    1308    1359    4098    4149    4392 
   Size:        493     815      51    2739      51     243

  Fragments arranged by size: 

                2739     815     493     243      51      51
 
PasI CC'CwG_GG

Cuts at:      0    3432    4392 
   Size:       3432     960
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4123    4392 
   Size:       4123     269
 
PfoI T'CCnGG_A

Cuts at:      0    1434    1815    2169    3236    4392 
   Size:       1434     381     354    1067    1156

  Fragments arranged by size: 

                1434    1156    1067     381     354
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1393    1425    4392 
   Size:       1393      32    2967

  Fragments arranged by size: 

                2967    1393      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     891    3058    4282    4392 
   Size:        891    2167    1224     110

  Fragments arranged by size: 

                2167    1224     891     110
 
PstI C_TGCA'G

Cuts at:      0    1530    1928    3207    4392 
   Size:       1530     398    1279    1185

  Fragments arranged by size: 

                1530    1279    1185     398
 
PvuII CAG'CTG

Cuts at:      0     271    1808    1929    2542    3208    3246    4338    4392 
   Size:        271    1537     121     613     666      38    1092      54

  Fragments arranged by size: 

                1537    1092     666     613     271     121      54      38
            
 
SacI G_AGCT'C

Cuts at:      0    3234    4392 
   Size:       3234    1158
 
SanDI GG'GwC_CC

Cuts at:      0    4282    4392 
   Size:       4282     110
 
SapI GCTCTTCn'nnn_

Cuts at:      0     449    1048    4392 
   Size:        449     599    3344

  Fragments arranged by size: 

                3344     599     449
 
SbfI CC_TGCA'GG

Cuts at:      0    1530    4392 
   Size:       1530    2862
 
ScaI AGT'ACT

Cuts at:      0     977    4392 
   Size:        977    3415
 
SexAI A'CCwGG_T

Cuts at:      0     893    4392 
   Size:        893    3499
 
SfcI C'TryA_G

Cuts at:      0     311     536    1526    1924    3125    3203    4392 
   Size:        311     225     990     398    1201      78    1189

  Fragments arranged by size: 

                1201    1189     990     398     311     225      78
 
SfoI GGC'GCC

Cuts at:      0    2484    3389    4392 
   Size:       2484     905    1003

  Fragments arranged by size: 

                2484    1003     905
 
SmlI C'TyrA_G

Cuts at:      0     358    2047    2296    3351    4392 
   Size:        358    1689     249    1055    1041

  Fragments arranged by size: 

                1689    1055    1041     358     249
 
SphI G_CATG'C

Cuts at:      0    1308    1359    4392 
   Size:       1308      51    3033

  Fragments arranged by size: 

                3033    1308      51
 
SspI AAT'ATT

Cuts at:      0    4387    4392 
   Size:       4387       5
 
StuI AGG'CCT

Cuts at:      0    3663    4392 
   Size:       3663     729
 
StyI C'CwwG_G

Cuts at:      0     828    1462    2784    2928    3103    3473    4178    4277 
   Size:        828     634    1322     144     175     370     705      99

Cuts at:   4277    4392 
   Size:        115

  Fragments arranged by size: 

                1322     828     705     634     370     175     144     115
                  99
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1524    4392 
   Size:       1524    2868
 
TatI w'GTAC_w

Cuts at:      0     975    4148    4392 
   Size:        975    3173     244

  Fragments arranged by size: 

                3173     975     244
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     929     961    2165    2197    2447    2479    4392 
   Size:        929      32    1204      32     250      32    1913

  Fragments arranged by size: 

                1913    1204     929     250      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2855    3372    4392 
   Size:       2855     517    1020

  Fragments arranged by size: 

                2855    1020     517
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AhdI     AlfI    AlwNI     ApoI     AseI
     AvaI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NsiI     NspI     PasI
    PflMI     PfoI     PpiI    PpuMI     PstI    PvuII     SacI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SmlI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AleI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BglII     BlpI     BmtI    BsaAI    BsaBI    BsiWI    BsrBI
   BssHII    BstBI  BstZ17I     ClaI     DraI   DraIII    EcoRI    EcoRV
     FalI     FseI    FspAI     KpnI     MluI     NaeI   NgoMIV     NheI
     NotI     NruI     PacI     PciI     PmeI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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