Restriction map for pF1KB0100
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BcgI        BclI        BfrI        BglII       
BlnI        BsaBI       BsiWI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MflI        MluI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccIII       1767  TCCGGA               T^CCGGA
AflIII       1356  ACACGT               A^C[GA][CT]GT
Alw44I       1480  GTGCAC               G^TGCAC
ApaLI        1480  GTGCAC               G^TGCAC
BbvCI         403  GCTGAGG              CCTCAGC(-5/-2)
BfaI         2953  CTAG                 C^TAG
BseAI        1767  TCCGGA               T^CCGGA
BsiEI        1847  CGGTCG               CG[GA][CT]^CG
BsmBI         730  GAGACG               CGTCTC(1/5)
BsmI          981  GAATGC               GAATGC(1/-1)
BspEI        1767  TCCGGA               T^CCGGA
BsrDI        2496  CATTGC               GCAATG(2/0)
BsrFI        1696  GCCGGC               [GA]^CCGG[CT]
BssSI         923  CACGAG               CACGAG(-5/-1)
BstEII       2211  GGTCACC              G^GT[ACGT]ACC
BstPI        2211  GGTCACC              G^GT[ACGT]ACC
Cfr10I       1696  GCCGGC               [GA]^CCGG[CT]
EcoO65I      2211  GGTCACC              G^GT[ACGT]ACC
MaeI         2953  CTAG                 C^TAG
MroI         1767  TCCGGA               T^CCGGA
MseI         1388  TTAA                 T^TAA
NaeI         1696  GCCGGC               GCC^GGC
NgoMIV       1696  GCCGGC               G^CCGGC
PflMI        2727  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1957  GACCCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1402  GAGCTC               GAGCT^C
SbfI         2772  CCTGCAGG             CCTGCA^GG
SexAI        1484  ACCAGGT              A^CC[AT]GGT
SmaI         1985  CCCGGG               CCC^GGG
SmlI         1742  CTCAAG               C^T[CT][GA]AG
SphI          337  GCATGC               GCATG^C
Sse8387I     2772  CCTGCAGG             CCTGCA^GG
TfiI         1594  GAATC                G^A[AT]TC
Tru9I        1388  TTAA                 T^TAA
Van91I       2727  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1985  CCCGGG               C^CCGGG
XmaI         1985  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII         292  CGCG                 CG^CG
              619  CGCG                
AlwNI        2104  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2350  CAGTACCTG           
BbeI         1166  GGCGCC               GGCGC^C
             2122  GGCGCC              
BciVI         910  GGATAC               GTATCC(6/5)
             2543  GGATAC              
BsaAI         628  TACGTG               [CT]AC^GT[GA]
             1357  CACGTA              
BsaI          376  GAGACC               GGTCTC(1/5)
             1621  GAGACC              
BsaWI         118  ACCGGA               [AT]^CCGG[AT]
             1767  TCCGGA              
BstUI         292  CGCG                 CG^CG
              619  CGCG                
EcoT14I       458  CCAAGG               C^C[AT][AT]GG
             1781  CCTTGG              
HaeII        1166  GGCGCC               [GA]GCGC^[CT]
             2122  GGCGCC              
HgaI          197  GACGC                GACGC(5/10)
              732  GACGC               
KasI         1166  GGCGCC               G^GCGCC
             2122  GGCGCC              
MfeI          261  CAATTG               C^AATTG
              470  CAATTG              
MunI          261  CAATTG               C^AATTG
              470  CAATTG              
MvnI          292  CGCG                 CG^CG
              619  CGCG                
NarI         1166  GGCGCC               GG^CGCC
             2122  GGCGCC              
NspI          220  GCATGT               [GA]CATG^[CT]
              337  GCATGC              
SapI          678  GCTCTTC              GCTCTTC(1/4)
             1203  GCTCTTC             
SfoI         1166  GGCGCC               GGC^GCC
             2122  GGCGCC              
StyI          458  CCAAGG               C^C[AT][AT]GG
             1781  CCTTGG              
Tsp509I       262  AATT                 ^AATT
              471  AATT                
XcmI          154  CCACCAGGGGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1832  CCATCACCGTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AocI        ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfiI        
StuI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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