Restriction map for pF1KB0100
 (Linear) (Six-base) MAPSORT of: KIBB0100.seq  Check: 1823  from: 1  to: 3063



KIBB0100.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     376     700    3063 
   Size:        376     324    2363

  Fragments arranged by size: 

                2363     376     324
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29      65    2913    3010    3063 
   Size:         29      36    2848      97      53

  Fragments arranged by size: 

                2848      97      53      36      29
 
AflIII A'CryG_T

Cuts at:      0    1356    3063 
   Size:       1356    1707
 
AleI CACnn'nnGTG

Cuts at:      0     784    3063 
   Size:        784    2279
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2281    2313    3063 
   Size:       2281      32     750

  Fragments arranged by size: 

                2281     750      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2109    2355    3063 
   Size:       2109     246     708

  Fragments arranged by size: 

                2109     708     246
 
ApaI G_GGCC'C

Cuts at:      0     240     300    1686    2461    3063 
   Size:        240      60    1386     775     602

  Fragments arranged by size: 

                1386     775     602     240      60
 
ApaLI G'TGCA_C

Cuts at:      0    1480    3063 
   Size:       1480    1583
 
AvaI C'yCGr_G

Cuts at:      0    1175    1918    1985    3063 
   Size:       1175     743      67    1078

  Fragments arranged by size: 

                1175    1078     743      67
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2536    2569    3063 
   Size:       2536      33     494

  Fragments arranged by size: 

                2536     494      33
 
BanI G'GyrC_C

Cuts at:      0     247     364     440     778    1166    2122    3063 
   Size:        247     117      76     338     388     956     941

  Fragments arranged by size: 

                 956     941     388     338     247     117      76
 
BanII G_rGCy'C

Cuts at:      0     240     300     413     962    1406    1654    1686    2461 
   Size:        240      60     113     549     444     248      32     775

Cuts at:   2461    3052    3063 
   Size:        591      11

  Fragments arranged by size: 

                 775     591     549     444     248     240     113      60
                  32      11
 
BbeI G_GCGC'C

Cuts at:      0    1170    2126    3063 
   Size:       1170     956     937

  Fragments arranged by size: 

                1170     956     937
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      71     962    1595    1748    1845    2963    3063 
   Size:         71     891     633     153      97    1118     100

  Fragments arranged by size: 

                1118     891     633     153     100      97      71
 
BbvCI CC'TCA_GC

Cuts at:      0     404    3063 
   Size:        404    2659
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     904    2537    3063 
   Size:        904    1633     526

  Fragments arranged by size: 

                1633     904     526
 
BglI GCCn_nnn'nGGC

Cuts at:      0     304     658     745    1055    1313    2104    2113    3063 
   Size:        304     354      87     310     258     791       9     950

  Fragments arranged by size: 

                 950     791     354     310     304     258      87       9
            
 
BlpI GC'TnA_GC

Cuts at:      0    1146    1405    1648    2942    2947    3063 
   Size:       1146     259     243    1294       5     116

  Fragments arranged by size: 

                1294    1146     259     243     116       5
 
Bme1580I G_kGCm'C

Cuts at:      0     240     300     781    1267    1356    1463    1484    1686 
   Size:        240      60     481     486      89     107      21     202

Cuts at:   1686    2461    2709    3063 
   Size:        775     248     354

  Fragments arranged by size: 

                 775     486     481     354     248     240     202     107
                  89      60      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      15    2595    3063 
   Size:         15    2580     468

  Fragments arranged by size: 

                2580     468      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     327    1072    2221    2553    2633    2905    3063 
   Size:        227     100     745    1149     332      80     272     158

  Fragments arranged by size: 

                1149     745     332     272     227     158     100      80
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     404    3063 
   Size:        404    2659
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1727    3063 
   Size:       1727    1336
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     370    1615    3063 
   Size:        370    1245    1448

  Fragments arranged by size: 

                1448    1245     370
 
BsaAI yAC'GTr

Cuts at:      0     630    1359    3063 
   Size:        630     729    1704

  Fragments arranged by size: 

                1704     729     630
 
BsaHI Gr'CG_yC

Cuts at:      0     198    1167    2123    3063 
   Size:        198     969     956     940

  Fragments arranged by size: 

                 969     956     940     198
 
BsaWI w'CCGG_w

Cuts at:      0     118    1767    3063 
   Size:        118    1649    1296

  Fragments arranged by size: 

                1649    1296     118
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     856     886    1089    1114    1119    1144    2281    2311 
   Size:        856      30     203      25       5      25    1137      30

Cuts at:   2311    2890    2920    3063 
   Size:        579      30     143

  Fragments arranged by size: 

                1137     856     579     203     143      30      30      30
                  25      25       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     421     486     489     492     495     498     501     504 
   Size:        421      65       3       3       3       3       3       3

Cuts at:    504     820     941    1409    2032    3063 
   Size:        316     121     468     623    1031

  Fragments arranged by size: 

                1031     623     468     421     316     121      65       3
                   3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     556     748     976    1026    1182    1760    2166    2477 
   Size:        556     192     228      50     156     578     406     311

Cuts at:   2477    3063 
   Size:        586

  Fragments arranged by size: 

                 586     578     556     406     311     228     192     156
                  50
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     835    1080    1399    2992    3063 
   Size:        835     245     319    1593      71

  Fragments arranged by size: 

                1593     835     319     245      71
 
BsiEI CG_ry'CG

Cuts at:      0    1850    3063 
   Size:       1850    1213
 
BsiHKAI G_wGCw'C

Cuts at:      0     707    1406    1484    3063 
   Size:        707     699      78    1579

  Fragments arranged by size: 

                1579     707     699      78
 
BsmI GAATG_Cn'

Cuts at:      0     987    3063 
   Size:        987    2076
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     724    3063 
   Size:        724    2339
 
Bsp1286I G_dGCh'C

Cuts at:      0     240     300     413     707     781     962    1267    1356 
   Size:        240      60     113     294      74     181     305      89

Cuts at:   1356    1406    1463    1484    1654    1686    2461    2709    3052 
   Size:         50      57      21     170      32     775     248     343

Cuts at:   3052    3063 
   Size:         11

  Fragments arranged by size: 

                 775     343     305     294     248     240     181     170
                 113      89      74      60      57      50      32      21
                  11
 
BspEI T'CCGG_A

Cuts at:      0    1767    3063 
   Size:       1767    1296
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     376     700    1208    1254    2969    3063 
   Size:        376     324     508      46    1715      94

  Fragments arranged by size: 

                1715     508     376     324      94      46
 
BsrBI CCG'CTC

Cuts at:      0     288     359     594    3063 
   Size:        288      71     235    2469

  Fragments arranged by size: 

                2469     288     235      71
 
BsrDI GCAATG_nn'

Cuts at:      0    2495    3063 
   Size:       2495     568
 
BsrFI r'CCGG_y

Cuts at:      0    1696    3063 
   Size:       1696    1367
 
BssSI C'ACGA_G

Cuts at:      0     923    3063 
   Size:        923    2140
 
BstEII G'GTnAC_C

Cuts at:      0    2211    3063 
   Size:       2211     852
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     164     556    1543    1666    2883    3063 
   Size:        164     392     987     123    1217     180

  Fragments arranged by size: 

                1217     987     392     180     164     123
 
Bsu36I CC'TnA_GG

Cuts at:      0    1421    2278    2453    3037    3063 
   Size:       1421     857     175     584      26

  Fragments arranged by size: 

                1421     857     584     175      26
 
BtgI C'CryG_G

Cuts at:      0     549     952    1701    2071    3063 
   Size:        549     403     749     370     992

  Fragments arranged by size: 

                 992     749     549     403     370
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2362    3063 
   Size:       2362     701
 
BtsI GCAGTG_nn'

Cuts at:      0     852    1628    2168    3063 
   Size:        852     776     540     895

  Fragments arranged by size: 

                 895     852     776     540
 
DraIII CAC_nnn'GTG

Cuts at:      0     282    1488    2400    2421    3063 
   Size:        282    1206     912      21     642

  Fragments arranged by size: 

                1206     912     642     282      21
 
EaeI y'GGCC_r

Cuts at:      0    1522    1656    1698    2096    2318    2442    2988    3063 
   Size:       1522     134      42     398     222     124     546      75

  Fragments arranged by size: 

                1522     546     398     222     134     124      75      42
            
 
EarI CTCTTCn'nnn_

Cuts at:      0      92     522     685    1210    3063 
   Size:         92     430     163     525    1853

  Fragments arranged by size: 

                1853     525     430     163      92
 
EcoICRI GAG'CTC

Cuts at:      0    1404    3063 
   Size:       1404    1659
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29      65     227     327    1072    2221    2553    2633 
   Size:         29      36     162     100     745    1149     332      80

Cuts at:   2633    2905    2913    3010    3063 
   Size:        272       8      97      53

  Fragments arranged by size: 

                1149     745     332     272     162     100      97      80
                  53      36      29       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0     236     237     296     544     650     755    1089    1197 
   Size:        236       1      59     248     106     105     334     108

Cuts at:   1197    1956    2106    2457    2787    2811    3063 
   Size:        759     150     351     330      24     252

  Fragments arranged by size: 

                 759     351     334     330     252     248     236     150
                 108     106     105      59      24       1
 
HaeII r_GCGC'y

Cuts at:      0    1170    2126    3063 
   Size:       1170     956     937

  Fragments arranged by size: 

                1170     956     937
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1725    1757    2890    2922    3063 
   Size:       1725      32    1133      32     141

  Fragments arranged by size: 

                1725    1133     141      32      32
 
KasI G'GCGC_C

Cuts at:      0    1166    2122    3063 
   Size:       1166     956     941

  Fragments arranged by size: 

                1166     956     941
 
MfeI C'AATT_G

Cuts at:      0     261     470    3063 
   Size:        261     209    2593

  Fragments arranged by size: 

                2593     261     209
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1100    1526    2663    3063 
   Size:       1100     426    1137     400

  Fragments arranged by size: 

                1137    1100     426     400
 
MscI TGG'CCA

Cuts at:      0    1658    2098    2320    2444    2990    3063 
   Size:       1658     440     222     124     546      73

  Fragments arranged by size: 

                1658     546     440     222     124      73
 
MslI CAynn'nnrTG

Cuts at:      0     784    1513    1664    1837    3063 
   Size:        784     729     151     173    1226

  Fragments arranged by size: 

                1226     784     729     173     151
 
MspA1I CmG'CkG

Cuts at:      0     564     772     899    1253    1277    1647    1860    1893 
   Size:        564     208     127     354      24     370     213      33

Cuts at:   1893    2286    2313    2328    2730    3063 
   Size:        393      27      15     402     333

  Fragments arranged by size: 

                 564     402     393     370     354     333     213     208
                 127      33      27      24      15
 
NaeI GCC'GGC

Cuts at:      0    1698    3063 
   Size:       1698    1365
 
NarI GG'CG_CC

Cuts at:      0    1167    2123    3063 
   Size:       1167     956     940

  Fragments arranged by size: 

                1167     956     940
 
NgoMIV G'CCGG_C

Cuts at:      0    1696    3063 
   Size:       1696    1367
 
NspI r_CATG'y

Cuts at:      0     224     341    3063 
   Size:        224     117    2722

  Fragments arranged by size: 

                2722     224     117
 
PasI CC'CwG_GG

Cuts at:      0    2791    2970    3063 
   Size:       2791     179      93

  Fragments arranged by size: 

                2791     179      93
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2733    3063 
   Size:       2733     330
 
PfoI T'CCnGG_A

Cuts at:      0     638    1553    2661    3063 
   Size:        638     915    1108     402

  Fragments arranged by size: 

                1108     915     638     402
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2281    2313    2614    2646    3063 
   Size:       2281      32     301      32     417

  Fragments arranged by size: 

                2281     417     301      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     544    1197    1956    2787    3063 
   Size:        544     653     759     831     276

  Fragments arranged by size: 

                 831     759     653     544     276
 
PshAI GACnn'nnGTC

Cuts at:      0    1961    3063 
   Size:       1961    1102
 
PspOMI G'GGCC_C

Cuts at:      0     236     296    1682    2457    3063 
   Size:        236      60    1386     775     606

  Fragments arranged by size: 

                1386     775     606     236      60
 
PstI C_TGCA'G

Cuts at:      0     531    1282    1431    1898    2069    2138    2312    2777 
   Size:        531     751     149     467     171      69     174     465

Cuts at:   2777    3063 
   Size:        286

  Fragments arranged by size: 

                 751     531     467     465     286     174     171     149
                  69
 
PvuII CAG'CTG

Cuts at:      0     772    1647    1893    2313    2730    3063 
   Size:        772     875     246     420     417     333

  Fragments arranged by size: 

                 875     772     420     417     333     246
 
SacI G_AGCT'C

Cuts at:      0    1406    3063 
   Size:       1406    1657
 
SanDI GG'GwC_CC

Cuts at:      0     544    2787    3063 
   Size:        544    2243     276

  Fragments arranged by size: 

                2243     544     276
 
SapI GCTCTTCn'nnn_

Cuts at:      0     685    1210    3063 
   Size:        685     525    1853

  Fragments arranged by size: 

                1853     685     525
 
SbfI CC_TGCA'GG

Cuts at:      0    2777    3063 
   Size:       2777     286
 
SexAI A'CCwGG_T

Cuts at:      0    1484    3063 
   Size:       1484    1579
 
SfcI C'TryA_G

Cuts at:      0     527    1278    1427    1894    2065    2134    2308    2773 
   Size:        527     751     149     467     171      69     174     465

Cuts at:   2773    3063 
   Size:        290

  Fragments arranged by size: 

                 751     527     467     465     290     174     171     149
                  69
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     658    2104    2113    3063 
   Size:        658    1446       9     950

  Fragments arranged by size: 

                1446     950     658       9
 
SfoI GGC'GCC

Cuts at:      0    1168    2124    3063 
   Size:       1168     956     939

  Fragments arranged by size: 

                1168     956     939
 
SmaI CCC'GGG

Cuts at:      0    1987    3063 
   Size:       1987    1076
 
SmlI C'TyrA_G

Cuts at:      0    1742    3063 
   Size:       1742    1321
 
SphI G_CATG'C

Cuts at:      0     341    3063 
   Size:        341    2722
 
StuI AGG'CCT

Cuts at:      0     937    1316    2802    2863    3063 
   Size:        937     379    1486      61     200

  Fragments arranged by size: 

                1486     937     379     200      61
 
StyI C'CwwG_G

Cuts at:      0     458    1781    3063 
   Size:        458    1323    1282

  Fragments arranged by size: 

                1323    1282     458
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      51     340     628    3063 
   Size:         51     289     288    2435

  Fragments arranged by size: 

                2435     289     288      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     856     888    1089    1121    1854    1886    1907    1939 
   Size:        856      32     201      32     733      32      21      32

Cuts at:   1939    3063 
   Size:       1124

  Fragments arranged by size: 

                1124     856     733     201      32      32      32      32
                  21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     161    1839    3063 
   Size:        161    1678    1224

  Fragments arranged by size: 

                1678    1224     161
 
XmaI C'CCGG_G

Cuts at:      0    1985    3063 
   Size:       1985    1078
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AloI    AlwNI     ApaI    ApaLI
     AvaI     BaeI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I
     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI
   PspOMI     PstI    PvuII     SacI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     ApoI     AscI     AseI    AsiSI    AvrII    BamHI     BcgI
     BclI    BfrBI    BglII    BmgBI     BmtI     BplI    BsaBI    BsiWI
    BspHI    BsrGI   BssHII   BstAPI    BstBI    BstYI  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     ScaI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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