Restriction map for pF1KA1687
 (Linear) (Six-base) MAPSORT of: KIAA1687.seq  Check: 2777  from: 1  to: 2154



KIAA1687.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     205     461     485    2154 
   Size:         91     114     256      24    1669

  Fragments arranged by size: 

                1669     256     114      91      24
 
AlwNI CAG_nnn'CTG

Cuts at:      0      10     610    1140    2154 
   Size:         10     600     530    1014

  Fragments arranged by size: 

                1014     600     530      10
 
ApoI r'AATT_y

Cuts at:      0     267     339     699     955     980    2154 
   Size:        267      72     360     256      25    1174

  Fragments arranged by size: 

                1174     360     267     256      72      25
 
AseI AT'TA_AT

Cuts at:      0    1010    2154 
   Size:       1010    1144
 
AvaI C'yCGr_G

Cuts at:      0     167    1976    2154 
   Size:        167    1809     178

  Fragments arranged by size: 

                1809     178     167
 
BanI G'GyrC_C

Cuts at:      0     222    1378    1959    2154 
   Size:        222    1156     581     195

  Fragments arranged by size: 

                1156     581     222     195
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      97     449     772    2154 
   Size:         97     352     323    1382

  Fragments arranged by size: 

                1382     352     323      97
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     487     521    1237    1271    2154 
   Size:        487      34     716      34     883

  Fragments arranged by size: 

                 883     716     487      34      34
 
BglII A'GATC_T

Cuts at:      0     460    1146    2154 
   Size:        460     686    1008

  Fragments arranged by size: 

                1008     686     460
 
Bme1580I G_kGCm'C

Cuts at:      0    1290    1999    2154 
   Size:       1290     709     155

  Fragments arranged by size: 

                1290     709     155
 
BmgBI CAC'GTC

Cuts at:      0    1732    1819    2154 
   Size:       1732      87     335

  Fragments arranged by size: 

                1732     335      87
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1099    2154 
   Size:       1099    1055
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     182    1991    2029    2154 
   Size:        182    1809      38     125

  Fragments arranged by size: 

                1809     182     125      38
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75    1884    2154 
   Size:         75    1809     270

  Fragments arranged by size: 

                1809     270      75
 
Bpu10I CC'TnA_GC

Cuts at:      0     852    1545    2154 
   Size:        852     693     609

  Fragments arranged by size: 

                 852     693     609
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1201    2154 
   Size:       1201     953
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1021    2154 
   Size:       1021    1133
 
BsaAI yAC'GTr

Cuts at:      0    1656    2019    2154 
   Size:       1656     363     135

  Fragments arranged by size: 

                1656     363     135
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1400    1430    1782    1812    1882    1912    2154 
   Size:       1400      30     352      30      70      30     242

  Fragments arranged by size: 

                1400     352     242      70      30      30      30
 
BseYI C'CCAG_C

Cuts at:      0     579    2154 
   Size:        579    1575
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730    1017    1090    2154 
   Size:        730     287      73    1064

  Fragments arranged by size: 

                1064     730     287      73
 
BsiHKAI G_wGCw'C

Cuts at:      0    1803    2154 
   Size:       1803     351
 
BsmI GAATG_Cn'

Cuts at:      0     456    2154 
   Size:        456    1698
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1445    2154 
   Size:       1445     709
 
Bsp1286I G_dGCh'C

Cuts at:      0    1290    1803    1999    2154 
   Size:       1290     513     196     155

  Fragments arranged by size: 

                1290     513     196     155
 
BspHI T'CATG_A

Cuts at:      0    1590    2154 
   Size:       1590     564
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     140    2154 
   Size:        140    2014
 
BsrDI GCAATG_nn'

Cuts at:      0     632    1893    2154 
   Size:        632    1261     261

  Fragments arranged by size: 

                1261     632     261
 
BsrFI r'CCGG_y

Cuts at:      0     237    2154 
   Size:        237    1917
 
BsrGI T'GTAC_A

Cuts at:      0     886    2154 
   Size:        886    1268
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1068    1294    2154 
   Size:       1068     226     860

  Fragments arranged by size: 

                1068     860     226
 
BstYI r'GATC_y

Cuts at:      0      38     460     684    1146    1237    2154 
   Size:         38     422     224     462      91     917

  Fragments arranged by size: 

                 917     462     422     224      91      38
 
Bsu36I CC'TnA_GG

Cuts at:      0      43    1351    1634    2154 
   Size:         43    1308     283     520

  Fragments arranged by size: 

                1308     520     283      43
 
BtsI GCAGTG_nn'

Cuts at:      0     611    1005    1057    2154 
   Size:        611     394      52    1097

  Fragments arranged by size: 

                1097     611     394      52
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1800    1835    2154 
   Size:       1800      35     319

  Fragments arranged by size: 

                1800     319      35
 
DraI TTT'AAA

Cuts at:      0    1460    2154 
   Size:       1460     694
 
EaeI y'GGCC_r

Cuts at:      0    1389    2154 
   Size:       1389     765
 
EarI CTCTTCn'nnn_

Cuts at:      0     151     461     509     656    1019    1443    2112    2154 
   Size:        151     310      48     147     363     424     669      42

  Fragments arranged by size: 

                 669     424     363     310     151     147      48      42
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75      91     205     461     485    1884    2154 
   Size:         75      16     114     256      24    1399     270

  Fragments arranged by size: 

                1399     270     256     114      75      24      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      77    2154 
   Size:         77    2077
 
EcoO109I rG'GnC_Cy

Cuts at:      0     134    1629    2154 
   Size:        134    1495     525

  Fragments arranged by size: 

                1495     525     134
 
EcoRI G'AATT_C

Cuts at:      0     955    2154 
   Size:        955    1199
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     796     828    1222    1254    1933    1965    2154 
   Size:        796      32     394      32     679      32     189

  Fragments arranged by size: 

                 796     679     394     189      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1529    1670    1952    2109    2154 
   Size:       1529     141     282     157      45

  Fragments arranged by size: 

                1529     282     157     141      45
 
HindIII A'AGCT_T

Cuts at:      0     353    2154 
   Size:        353    1801
 
HpaI GTT'AAC

Cuts at:      0    2109    2154 
   Size:       2109      45
 
MfeI C'AATT_G

Cuts at:      0     736    2154 
   Size:        736    1418
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1913    2004    2154 
   Size:       1913      91     150

  Fragments arranged by size: 

                1913     150      91
 
MslI CAynn'nnrTG

Cuts at:      0    1012    2154 
   Size:       1012    1142
 
MspA1I CmG'CkG

Cuts at:      0     789    2154 
   Size:        789    1365
 
NdeI CA'TA_TG

Cuts at:      0    2067    2154 
   Size:       2067      87
 
NspI r_CATG'y

Cuts at:      0    2132    2154 
   Size:       2132      22
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     591    1364    2154 
   Size:        591     773     790

  Fragments arranged by size: 

                 790     773     591
 
PpuMI rG'GwC_Cy

Cuts at:      0     134    1629    2154 
   Size:        134    1495     525

  Fragments arranged by size: 

                1495     525     134
 
PshAI GACnn'nnGTC

Cuts at:      0     796    2154 
   Size:        796    1358
 
PsiI TTA'TAA

Cuts at:      0     484    2154 
   Size:        484    1670
 
PstI C_TGCA'G

Cuts at:      0     774     788    2154 
   Size:        774      14    1366

  Fragments arranged by size: 

                1366     774      14
 
PvuII CAG'CTG

Cuts at:      0     789    2154 
   Size:        789    1365
 
SanDI GG'GwC_CC

Cuts at:      0     134    1629    2154 
   Size:        134    1495     525

  Fragments arranged by size: 

                1495     525     134
 
SapI GCTCTTCn'nnn_

Cuts at:      0     151     461    2112    2154 
   Size:        151     310    1651      42

  Fragments arranged by size: 

                1651     310     151      42
 
SfcI C'TryA_G

Cuts at:      0     413     770     784    1298    1580    1616    2154 
   Size:        413     357      14     514     282      36     538

  Fragments arranged by size: 

                 538     514     413     357     282      36      14
 
SmlI C'TyrA_G

Cuts at:      0    1180    1976    2154 
   Size:       1180     796     178

  Fragments arranged by size: 

                1180     796     178
 
SnaBI TAC'GTA

Cuts at:      0    1656    2154 
   Size:       1656     498
 
StyI C'CwwG_G

Cuts at:      0     704     879    2154 
   Size:        704     175    1275

  Fragments arranged by size: 

                1275     704     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1044    2154 
   Size:       1044    1110
 
TatI w'GTAC_w

Cuts at:      0     886    2154 
   Size:        886    1268
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1782    1814    2154 
   Size:       1782      32     340

  Fragments arranged by size: 

                1782     340      32
 
XhoI C'TCGA_G

Cuts at:      0    1976    2154 
   Size:       1976     178
 
XmnI GAAnn'nnTTC

Cuts at:      0     343    2154 
   Size:        343    1811
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AseI     AvaI     BanI     BbsI     BcgI
    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaXI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstYI
   Bsu36I     BtsI    CspCI     DraI     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI
     MslI   MspA1I     NdeI     NspI    PflMI    PpuMI    PshAI     PsiI
     PstI    PvuII    SanDI     SapI     SfcI     SmlI    SnaBI     StyI
    TaqII     TatI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI    BciVI
     BclI    BfrBI     BglI     BlpI     BmtI    BsaBI    BsaHI    BsaWI
    BseRI    BsiEI    BsiWI    BspEI    BsrBI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XcmI     XmaI     ZraI
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