Restriction map for pF1KA1525
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# NO CUT ENZYMES (   82 enzymes) #
##################################

Acc65I      AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsiEI       BsiWI       BsmI        BspDI       
BsrBI       BssHII      BssSI       BstXI       Bsu36I      
CelII       ClaI        CpoI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       FseI        
FspI        HincII      HindII      HpaI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   69 enzymes) #
###################################

AatII        2988  GACGTC               GACGT^C
AccIII       2826  TCCGGA               T^CCGGA
AclI         2147  AACGTT               AA^CGTT
AflII        1596  CTTAAG               C^TTAAG
AflIII        728  ACATGT               A^C[GA][CT]GT
AgeI         3145  ACCGGT               A^CCGGT
AhdI         3277  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1252  GTGCAC               G^TGCAC
ApaLI        1252  GTGCAC               G^TGCAC
AspEI        3277  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1966  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           39  CCCGAG               C^[CT]CG[GA]G
BalI          144  TGGCCA               TGG^CCA
BanI         2041  GGCGCC               G^G[CT][GA]CC
BbeI         2041  GGCGCC               GGCGC^C
BbsI         3266  GAAGAC               GAAGAC(2/6)
BciVI         884  GGATAC               GTATCC(6/5)
BclI         1874  TGATCA               T^GATCA
BcnI          374  CCCGG                CC^[GC]GG
BfrI         1596  CTTAAG               C^TTAAG
BglII        2238  AGATCT               A^GATCT
BpmI         2409  CTCCAG               CTGGAG(16/14)
BpuAI        3266  GAAGAC               GAAGAC(2/6)
BseAI        2826  TCCGGA               T^CCGGA
BsoBI          39  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     2930  TGTACA               T^GTACA
BspEI        2826  TCCGGA               T^CCGGA
BspHI        1298  TCATGA               T^CATGA
BspLU11I      728  ACATGT               A^CATGT
BspMI        2004  GCAGGT               ACCTGC(4/8)
BsrFI        3145  ACCGGT               [GA]^CCGG[CT]
BsrGI        2930  TGTACA               T^GTACA
Bst1107I      996  GTATAC               GTA^TAC
BstBI         863  TTCGAA               TT^CGAA
BstEII       3353  GGTCACC              G^GT[ACGT]ACC
BstPI        3353  GGTCACC              G^GT[ACGT]ACC
BstZ17I       996  GTATAC               GTA^TAC
Cfr10I       3145  ACCGGT               [GA]^CCGG[CT]
DraI          499  TTTAAA               TTT^AAA
DraIII       2951  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2348  GACCTCCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          144  TGGCCA               [CT]^GGCC[GA]
Eam1105I     3277  GACAGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3353  GGTCACC              G^GT[ACGT]ACC
FauI         2033  GCGGG                CCCGC(4/6)
FbaI         1874  TGATCA               T^GATCA
HaeII        2041  GGCGCC               [GA]GCGC^[CT]
KasI         2041  GGCGCC               G^GCGCC
MluNI         144  TGGCCA               TGG^CCA
MroI         2826  TCCGGA               T^CCGGA
MscI          144  TGGCCA               TGG^CCA
NarI         2041  GGCGCC               GG^CGCC
NciI          374  CCCGG                CC^[GC]GG
NdeI         1235  CATATG               CA^TATG
NspV          863  TTCGAA               TT^CGAA
PflFI        1966  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         523  CCAGAGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        3145  ACCGGT               A^CCGGT
Psp1406I     2147  AACGTT               AA^CGTT
PvuII         560  CAGCTG               CAG^CTG
RcaI         1298  TCATGA               T^CATGA
ScaI         1627  AGTACT               AGT^ACT
SexAI        2979  ACCTGGT              A^CC[AT]GGT
SfoI         2041  GGCGCC               GGC^GCC
SfuI          863  TTCGAA               TT^CGAA
SspBI        2930  TGTACA               T^GTACA
SspI          438  AATATT               AAT^ATT
Tth111I      1966  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        523  CCAGAGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII        2200  CGCG                 CG^CG
             2622  CGCG                
AcyI         2041  GGCGCC               G[GA]^CG[CT]C
             2988  GACGTC              
BsaHI        2041  GGCGCC               G[GA]^CG[CT]C
             2988  GACGTC              
BsmBI        1948  CGTCTC               CGTCTC(1/5)
             2990  CGTCTC              
BstAPI       1983  GCAGGAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2095  GCAGATGCTGC         
BstUI        2200  CGCG                 CG^CG
             2622  CGCG                
CfoI         1794  GCGC                 GCG^C
             2042  GCGC                
EcoNI        1275  CCTCTGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2899  CCTTCGCAAGG         
EcoT22I        11  ATGCAT               ATGCA^T
              296  ATGCAT              
HgaI         2885  GACGC                GACGC(5/10)
              216  GCGTC               
HhaI         1794  GCGC                 GCG^C
             2042  GCGC                
Hin1I        2041  GGCGCC               G[GA]^CG[CT]C
             2988  GACGTC              
HinP1I       1794  GCGC                 G^CGC
             2042  GCGC                
HindIII       782  AAGCTT               A^AGCTT
             1262  AAGCTT              
MspA1I        560  CAGCTG               C[AC]G^C[GT]G
             3103  CAGCGG              
MvnI         2200  CGCG                 CG^CG
             2622  CGCG                
NsiI           11  ATGCAT               ATGCA^T
              296  ATGCAT              
PpuMI          82  GGGACCT              [GA]G^G[AT]CC[CT]
             1505  GGGACCT             
SapI          814  GAAGAGC              GCTCTTC(1/4)
             1519  GAAGAGC             


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BanII       BbvI        BfaI        BglI        BmyI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoRV       EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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