Restriction map for pF1KA1525
 (Linear) (Six-base) MAPSORT of: KIAA1525.seq  Check: 1554  from: 1  to: 3396



KIAA1525.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1995    3396 
   Size:       1995    1401
 
AatII G_ACGT'C

Cuts at:      0    2992    3396 
   Size:       2992     404
 
AccI GT'mk_AC

Cuts at:      0     431     997    2444    2678    3396 
   Size:        431     566    1447     234     718

  Fragments arranged by size: 

                1447     718     566     431     234
 
AclI AA'CG_TT

Cuts at:      0    2148    3396 
   Size:       2148    1248
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746    1901    2726    3260    3396 
   Size:        746    1155     825     534     136

  Fragments arranged by size: 

                1155     825     746     534     136
 
AflII C'TTAA_G

Cuts at:      0    1596    3396 
   Size:       1596    1800
 
AflIII A'CryG_T

Cuts at:      0     728    3396 
   Size:        728    2668
 
AgeI A'CCGG_T

Cuts at:      0    3145    3396 
   Size:       3145     251
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3282    3396 
   Size:       3282     114
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1712    3396 
   Size:       1712    1684
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1370    1402    3396 
   Size:       1370      32    1994

  Fragments arranged by size: 

                1994    1370      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     769    1989    2101    2569    2704    2733    3396 
   Size:        769    1220     112     468     135      29     663

  Fragments arranged by size: 

                1220     769     663     468     135     112      29
 
ApaLI G'TGCA_C

Cuts at:      0    1252    3396 
   Size:       1252    2144
 
ApoI r'AATT_y

Cuts at:      0      54     676     690     867    1141    1285    1934    2060 
   Size:         54     622      14     177     274     144     649     126

Cuts at:   2060    2131    3396 
   Size:         71    1265

  Fragments arranged by size: 

                1265     649     622     274     177     144     126      71
                  54      14
 
AvaI C'yCGr_G

Cuts at:      0      39    3396 
   Size:         39    3357
 
BanI G'GyrC_C

Cuts at:      0    2041    3396 
   Size:       2041    1355
 
BanII G_rGCy'C

Cuts at:      0     821    1526    3303    3396 
   Size:        821     705    1777      93

  Fragments arranged by size: 

                1777     821     705      93
 
BbeI G_GCGC'C

Cuts at:      0    2045    3396 
   Size:       2045    1351
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3273    3396 
   Size:       3273     123
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     878    3396 
   Size:        878    2518
 
BclI T'GATC_A

Cuts at:      0    1874    3396 
   Size:       1874    1522
 
BfrBI ATG'CAT

Cuts at:      0      13     298    3396 
   Size:         13     285    3098

  Fragments arranged by size: 

                3098     285      13
 
BglI GCCn_nnn'nGGC

Cuts at:      0     358     478     832    3396 
   Size:        358     120     354    2564

  Fragments arranged by size: 

                2564     358     354     120
 
BglII A'GATC_T

Cuts at:      0    2238    3396 
   Size:       2238    1158
 
Bme1580I G_kGCm'C

Cuts at:      0    1256    3396 
   Size:       1256    2140
 
BmgBI CAC'GTC

Cuts at:      0    1005    3396 
   Size:       1005    2391
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2348    2434    2506    3396 
   Size:       2348      86      72     890

  Fragments arranged by size: 

                2348     890      86      72
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     283    1206    3396 
   Size:        283     923    2190

  Fragments arranged by size: 

                2190     923     283
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2394    3396 
   Size:       2394    1002
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618    1201    2632    3396 
   Size:        618     583    1431     764

  Fragments arranged by size: 

                1431     764     618     583
 
BsaHI Gr'CG_yC

Cuts at:      0    2042    2989    3396 
   Size:       2042     947     407

  Fragments arranged by size: 

                2042     947     407
 
BsaWI w'CCGG_w

Cuts at:      0    2826    3145    3259    3396 
   Size:       2826     319     114     137

  Fragments arranged by size: 

                2826     319     137     114
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1038    1068    3396 
   Size:       1038      30    2328

  Fragments arranged by size: 

                2328    1038      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256    1622    1942    3396 
   Size:        256    1366     320    1454

  Fragments arranged by size: 

                1454    1366     320     256
 
BseYI C'CCAG_C

Cuts at:      0    2301    2726    3101    3396 
   Size:       2301     425     375     295

  Fragments arranged by size: 

                2301     425     375     295
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     830    2029    2450    3396 
   Size:        830    1199     421     946

  Fragments arranged by size: 

                1199     946     830     421
 
BsiHKAI G_wGCw'C

Cuts at:      0     821    1256    1526    3303    3396 
   Size:        821     435     270    1777      93

  Fragments arranged by size: 

                1777     821     435     270      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1954    2996    3396 
   Size:       1954    1042     400

  Fragments arranged by size: 

                1954    1042     400
 
Bsp1286I G_dGCh'C

Cuts at:      0     821    1256    1526    3303    3396 
   Size:        821     435     270    1777      93

  Fragments arranged by size: 

                1777     821     435     270      93
 
BspEI T'CCGG_A

Cuts at:      0    2826    3396 
   Size:       2826     570
 
BspHI T'CATG_A

Cuts at:      0    1298    3396 
   Size:       1298    2098
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1995    3396 
   Size:       1995    1401
 
BsrDI GCAATG_nn'

Cuts at:      0     379    1175    1613    3396 
   Size:        379     796     438    1783

  Fragments arranged by size: 

                1783     796     438     379
 
BsrFI r'CCGG_y

Cuts at:      0    3145    3396 
   Size:       3145     251
 
BsrGI T'GTAC_A

Cuts at:      0    2930    3396 
   Size:       2930     466
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1989    2101    3396 
   Size:       1989     112    1295

  Fragments arranged by size: 

                1989    1295     112
 
BstBI TT'CG_AA

Cuts at:      0     864    3396 
   Size:        864    2532
 
BstEII G'GTnAC_C

Cuts at:      0    3353    3396 
   Size:       3353      43
 
BstYI r'GATC_y

Cuts at:      0    1532    1785    2238    3256    3396 
   Size:       1532     253     453    1018     140

  Fragments arranged by size: 

                1532    1018     453     253     140
 
BstZ17I GTA'TAC

Cuts at:      0     998    3396 
   Size:        998    2398
 
BtgI C'CryG_G

Cuts at:      0     141    1109    3294    3396 
   Size:        141     968    2185     102

  Fragments arranged by size: 

                2185     968     141     102
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2604    3396 
   Size:       2604     792
 
BtsI GCAGTG_nn'

Cuts at:      0     505    1466    2486    3396 
   Size:        505     961    1020     910

  Fragments arranged by size: 

                1020     961     910     505
 
DraI TTT'AAA

Cuts at:      0     501    3396 
   Size:        501    2895
 
DraIII CAC_nnn'GTG

Cuts at:      0    2956    3396 
   Size:       2956     440
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2354    3396 
   Size:       2354    1042
 
EaeI y'GGCC_r

Cuts at:      0     144    3396 
   Size:        144    3252
 
EarI CTCTTCn'nnn_

Cuts at:      0     809    1514    1729    3245    3396 
   Size:        809     705     215    1516     151

  Fragments arranged by size: 

                1516     809     705     215     151
 
EcoICRI GAG'CTC

Cuts at:      0     819    1524    3301    3396 
   Size:        819     705    1777      95

  Fragments arranged by size: 

                1777     819     705      95
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746    1901    2394    2726    3260    3396 
   Size:        746    1155     493     332     534     136

  Fragments arranged by size: 

                1155     746     534     493     332     136
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1279    2903    3396 
   Size:       1279    1624     493

  Fragments arranged by size: 

                1624    1279     493
 
EcoO109I rG'GnC_Cy

Cuts at:      0      83    1506    2015    2719    3396 
   Size:         83    1423     509     704     677

  Fragments arranged by size: 

                1423     704     677     509      83
 
EcoRV GAT'ATC

Cuts at:      0     654    1173    1386    3396 
   Size:        654     519     213    2010

  Fragments arranged by size: 

                2010     654     519     213
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1405    1931    3387    3396 
   Size:       1405     526    1456       9

  Fragments arranged by size: 

                1456    1405     526       9
 
HaeII r_GCGC'y

Cuts at:      0    2045    3396 
   Size:       2045    1351
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     307     339    1215    1247    1614    1646    2230    2262 
   Size:        307      32     876      32     367      32     584      32

Cuts at:   2262    3009    3041    3268    3300    3396 
   Size:        747      32     227      32      96

  Fragments arranged by size: 

                 876     747     584     367     307     227      96      32
                  32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     782    1262    3396 
   Size:        782     480    2134

  Fragments arranged by size: 

                2134     782     480
 
KasI G'GCGC_C

Cuts at:      0    2041    3396 
   Size:       2041    1355
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     199    3396 
   Size:        199    3197
 
MscI TGG'CCA

Cuts at:      0     146    3396 
   Size:        146    3250
 
MslI CAynn'nnrTG

Cuts at:      0     146    1235    2623    3080    3396 
   Size:        146    1089    1388     457     316

  Fragments arranged by size: 

                1388    1089     457     316     146
 
MspA1I CmG'CkG

Cuts at:      0     562    3105    3396 
   Size:        562    2543     291

  Fragments arranged by size: 

                2543     562     291
 
NarI GG'CG_CC

Cuts at:      0    2042    3396 
   Size:       2042    1354
 
NcoI C'CATG_G

Cuts at:      0     141    1109    3294    3396 
   Size:        141     968    2185     102

  Fragments arranged by size: 

                2185     968     141     102
 
NdeI CA'TA_TG

Cuts at:      0    1236    3396 
   Size:       1236    2160
 
NsiI A_TGCA'T

Cuts at:      0      15     300    3396 
   Size:         15     285    3096

  Fragments arranged by size: 

                3096     285      15
 
NspI r_CATG'y

Cuts at:      0     732    1152    3233    3396 
   Size:        732     420    2081     163

  Fragments arranged by size: 

                2081     732     420     163
 
PasI CC'CwG_GG

Cuts at:      0    3288    3396 
   Size:       3288     108
 
PciI A'CATG_T

Cuts at:      0     728    3396 
   Size:        728    2668
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     529    3396 
   Size:        529    2867
 
PpuMI rG'GwC_Cy

Cuts at:      0      83    1506    3396 
   Size:         83    1423    1890

  Fragments arranged by size: 

                1890    1423      83
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1559    1591    3396 
   Size:       1559      32    1805

  Fragments arranged by size: 

                1805    1559      32
 
PstI C_TGCA'G

Cuts at:      0    1985    2109    2223    2493    3177    3396 
   Size:       1985     124     114     270     684     219

  Fragments arranged by size: 

                1985     684     270     219     124     114
 
PvuII CAG'CTG

Cuts at:      0     562    3396 
   Size:        562    2834
 
SacI G_AGCT'C

Cuts at:      0     821    1526    3303    3396 
   Size:        821     705    1777      93

  Fragments arranged by size: 

                1777     821     705      93
 
SapI GCTCTTCn'nnn_

Cuts at:      0     809    1514    3396 
   Size:        809     705    1882

  Fragments arranged by size: 

                1882     809     705
 
ScaI AGT'ACT

Cuts at:      0    1629    3396 
   Size:       1629    1767
 
SexAI A'CCwGG_T

Cuts at:      0    2979    3396 
   Size:       2979     417
 
SfcI C'TryA_G

Cuts at:      0    1029    1366    1981    2105    2219    2489    2675    3173 
   Size:       1029     337     615     124     114     270     186     498

Cuts at:   3173    3396 
   Size:        223

  Fragments arranged by size: 

                1029     615     498     337     270     223     186     124
                 114
 
SfoI GGC'GCC

Cuts at:      0    2043    3396 
   Size:       2043    1353
 
SmlI C'TyrA_G

Cuts at:      0     633    1216    1596    2647    3396 
   Size:        633     583     380    1051     749

  Fragments arranged by size: 

                1051     749     633     583     380
 
SspI AAT'ATT

Cuts at:      0     440    3396 
   Size:        440    2956
 
StyI C'CwwG_G

Cuts at:      0     141    1109    3294    3396 
   Size:        141     968    2185     102

  Fragments arranged by size: 

                2185     968     141     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     263     926    1239    2617    3396 
   Size:        263     663     313    1378     779

  Fragments arranged by size: 

                1378     779     663     313     263
 
TatI w'GTAC_w

Cuts at:      0     410     421    1627    2209    2930    3396 
   Size:        410      11    1206     582     721     466

  Fragments arranged by size: 

                1206     721     582     466     410      11
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1038    1070    3396 
   Size:       1038      32    2326

  Fragments arranged by size: 

                2326    1038      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1969    3396 
   Size:       1969    1427
 
ZraI GAC'GTC

Cuts at:      0    2990    3396 
   Size:       2990     406
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI
    HaeII    Hin4I  HindIII     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NsiI     NspI     PasI     PciI    PflMI
    PpuMI     PsrI     PstI    PvuII     SacI     SapI     ScaI    SexAI
     SfcI     SfoI     SmlI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI     AleI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI     BlpI     BmtI   Bpu10I     BsaI
    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BsrBI   BssHII    BssSI
    BstXI   Bsu36I     ClaI    CspCI     EagI     EciI    EcoRI     FseI
     FspI    FspAI   HincII     HpaI     KpnI     MfeI     MluI     NaeI
   NgoMIV     NheI     NotI     NruI     PacI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     XbaI     XcmI     XhoI
     XmaI     XmnI
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