Restriction map for pF1KA1520
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        AocI        ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BamHI       BbrPI       BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsiWI       BsmBI       BspDI       BsrDI       BssHII      
Bst1107I    BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        HpaI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MseI        MunI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SfiI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI         1393  GTCTAC               GT^[AC][GT]AC
AccIII       1133  TCCGGA               T^CCGGA
AflIII       1226  ACATGT               A^C[GA][CT]GT
Alw44I       1468  GTGCAC               G^TGCAC
Aor51HI      2011  AGCGCT               AGC^GCT
ApaLI        1468  GTGCAC               G^TGCAC
Asp700I      1431  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          892  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         320  TGCGCA               TGC^GCA
BbeI         1918  GGCGCC               GGCGC^C
BbvCI         463  GCTGAGG              CCTCAGC(-5/-2)
BlpI         2100  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2100  GCTGAGC              GC^T[ACGT]AGC
BsaWI        1133  TCCGGA               [AT]^CCGG[AT]
BseAI        1133  TCCGGA               T^CCGGA
BsmI         1074  GAATGC               GAATGC(1/-1)
Bsp1407I     1193  TGTACA               T^GTACA
BspEI        1133  TCCGGA               T^CCGGA
BspLU11I     1226  ACATGT               A^CATGT
BsrGI        1193  TGTACA               T^GTACA
BstAPI        522  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         782  TTCGAA               TT^CGAA
BstEII        990  GGTCACC              G^GT[ACGT]ACC
BstPI         990  GGTCACC              G^GT[ACGT]ACC
BstYI         144  GGATCT               [GA]^GATC[CT]
CelII        2100  GCTGAGC              GC^T[ACGT]AGC
DraIII       1735  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          483  CGGCCG               C^GGCCG
EclXI         483  CGGCCG               C^GGCCG
Eco47III     2011  AGCGCT               AGC^GCT
Eco52I        483  CGGCCG               C^GGCCG
EcoO65I       990  GGTCACC              G^GT[ACGT]ACC
FseI          484  GGCCGGCC             GGCCGG^CC
FspI          320  TGCGCA               TGC^GCA
HgaI          505  GCGTC                GACGC(5/10)
HincII       1645  GTCAAC               GT[CT]^[GA]AC
HindII       1645  GTCAAC               GT[CT]^[GA]AC
HindIII       822  AAGCTT               A^AGCTT
KasI         1918  GGCGCC               G^GCGCC
MflI          144  GGATCT               [GA]^GATC[CT]
MroI         1133  TCCGGA               T^CCGGA
NaeI          485  GCCGGC               GCC^GGC
NarI         1918  GGCGCC               GG^CGCC
NgoMIV        485  GCCGGC               G^CCGGC
NspI         1226  ACATGT               [GA]CATG^[CT]
NspV          782  TTCGAA               TT^CGAA
PflFI         892  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         432  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         1635  GAGCTC               GAGCT^C
SexAI         893  ACCTGGT              A^CC[AT]GGT
SfoI         1918  GGCGCC               GGC^GCC
SfuI          782  TTCGAA               TT^CGAA
SmaI         1961  CCCGGG               CCC^GGG
SspBI        1193  TGTACA               T^GTACA
Tth111I       892  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        432  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         144  GGATCT               [GA]^GATC[CT]
XmaCI        1961  CCCGGG               C^CCGGG
XmaI         1961  CCCGGG               C^CCGGG
XmnI         1431  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcyI          504  GGCGTC               G[GA]^CG[CT]C
             1918  GGCGCC              
BglI          225  GCCCCGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              454  GCCACCGAGGC         
BsaHI         504  GGCGTC               G[GA]^CG[CT]C
             1918  GGCGCC              
BsgI         2155  GTGCAG               GTGCAG(16/14)
             2212  GTGCAG              
BsiEI         483  CGGCCG               CG[GA][CT]^CG
             1449  CGACCG              
BspHI          38  TCATGA               T^CATGA
             1016  TCATGA              
BsrI          804  ACTGG                ACTGG(1/-1)
             1984  CCAGT               
BstXI         194  CCATTGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2009  CCAGCGCTTTGG        
EcoNI          99  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1404  CCTGATGCAGG         
Hin1I         504  GGCGTC               G[GA]^CG[CT]C
             1918  GGCGCC              
HinfI         133  GACTC                G^A[ACGT]TC
             1378  GAGTC               
PleI          133  GACTC                GAGTC(4/5)
             1378  GAGTC               
RcaI           38  TCATGA               T^CATGA
             1016  TCATGA              
SapI         1632  GAAGAGC              GCTCTTC(1/4)
              306  GCTCTTC             
ScaI         1043  AGTACT               AGT^ACT
             1727  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcgI        BcnI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BssKI       BssSI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI        
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