Restriction map for
pF1KA1449
(Circular) (Six-base) MAPSORT of: pF1KA1449.seq Check: 3385 from: 1 to: 5144
pF1KA1449 5144 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1063 1373 2182 1063
Size: 310 809 4025
Fragments arranged by size:
4025 809 310
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 4984 4984
Size: 5144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1814 2969 3401 4148 4416 1814
Size: 1155 432 747 268 2542
Fragments arranged by size:
2542 1155 747 432 268
AflIII A'CryG_T
Cuts at: 703 903 1539 1902 2496 3600 4577 703
Size: 200 636 363 594 1104 977 1270
Fragments arranged by size:
1270 1104 977 636 594 363 200
AgeI A'CCGG_T
Cuts at: 3532 3532
Size: 5144
AleI CACnn'nnGTG
Cuts at: 904 904
Size: 5144
AlwNI CAG_nnn'CTG
Cuts at: 2449 4016 2449
Size: 1567 3577
ApaLI G'TGCA_C
Cuts at: 3914 3914
Size: 5144
ApoI r'AATT_y
Cuts at: 1091 1488 1789 1799 2148 4497 4753 1091
Size: 397 301 10 349 2349 256 1482
Fragments arranged by size:
2349 1482 397 349 301 256 10
AseI AT'TA_AT
Cuts at: 20 376 20
Size: 356 4788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 2164 2164
Size: 5144
BamHI G'GATC_C
Cuts at: 222 693 2052 2169 222
Size: 471 1359 117 3197
Fragments arranged by size:
3197 1359 471 117
BanI G'GyrC_C
Cuts at: 2160 2574 2822 2857 2160
Size: 414 248 35 4447
Fragments arranged by size:
4447 414 248 35
BanII G_rGCy'C
Cuts at: 2158 3188 2158
Size: 1030 4114
BbeI G_GCGC'C
Cuts at: 2578 2826 2578
Size: 248 4896
BciVI GTATCCnnnnn_n'
Cuts at: 3042 3803 5144 3042
Size: 761 1341 3042
Fragments arranged by size:
3042 1341 761
BclI T'GATC_A
Cuts at: 2664 2664
Size: 5144
BfrBI ATG'CAT
Cuts at: 1825 1825
Size: 5144
BglI GCCn_nnn'nGGC
Cuts at: 112 788 112
Size: 676 4468
BglII A'GATC_T
Cuts at: 1296 2659 4696 1296
Size: 1363 2037 1744
Fragments arranged by size:
2037 1744 1363
BlpI GC'TnA_GC
Cuts at: 2256 2256
Size: 5144
Bme1580I G_kGCm'C
Cuts at: 734 1637 2769 2862 3918 734
Size: 903 1132 93 1056 1960
Fragments arranged by size:
1960 1132 1056 903 93
BmrI ACTGGGnnnn_n'
Cuts at: 652 2356 2437 2772 4916 652
Size: 1704 81 335 2144 880
Fragments arranged by size:
2144 1704 880 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3511 3511
Size: 5144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1688 1133
Size: 555 4589
Bpu10I CC'TnA_GC
Cuts at: 474 474
Size: 5144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 2320 3199 3691 3989 985
Size: 1335 879 492 298 2140
Fragments arranged by size:
2140 1335 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2099 4848 2099
Size: 2749 2395
BsaAI yAC'GTr
Cuts at: 465 2497 465
Size: 2032 3112
BsaBI GATnn'nnATC
Cuts at: 6 2658 4695 6
Size: 2652 2037 455
Fragments arranged by size:
2652 2037 455
BsaHI Gr'CG_yC
Cuts at: 2575 2823 3525 5021 2575
Size: 248 702 1496 2698
Fragments arranged by size:
2698 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2333 2854 3532 3806 3953 4364 2333
Size: 521 678 274 147 411 3113
Fragments arranged by size:
3113 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1490 1520 1490
Size: 30 5114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1022 2029 1022
Size: 1007 4137
BseYI C'CCAG_C
Cuts at: 511 2569 3327 3904 4400 511
Size: 2058 758 577 496 1255
Fragments arranged by size:
2058 1255 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1627 172
Size: 1455 3689
BsiEI CG_ry'CG
Cuts at: 104 255 2686 2732 3940 104
Size: 151 2431 46 1208 1308
Fragments arranged by size:
2431 1308 1208 151 46
BsiHKAI G_wGCw'C
Cuts at: 2158 2936 3126 3918 2158
Size: 778 190 792 3384
Fragments arranged by size:
3384 792 778 190
BsmI GAATG_Cn'
Cuts at: 420 420
Size: 5144
Bsp1286I G_dGCh'C
Cuts at: 734 1637 2158 2769 2862 2936 3126 3188 3918
Size: 903 521 611 93 74 190 62 730
Cuts at: 3918 734
Size: 1960
Fragments arranged by size:
1960 903 730 611 521 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4364 4364
Size: 5144
BspHI T'CATG_A
Cuts at: 4320 4320
Size: 5144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 144 2194 2408 2710 3091 144
Size: 2050 214 302 381 2197
Fragments arranged by size:
2197 2050 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2099 3436 3490 4974 5139 50
Size: 2049 1337 54 1484 165 55
Fragments arranged by size:
2049 1484 1337 165 55 54
BsrDI GCAATG_nn'
Cuts at: 751 784 3056 751
Size: 33 2272 2839
Fragments arranged by size:
2839 2272 33
BsrFI r'CCGG_y
Cuts at: 122 250 3142 3323 3532 122
Size: 128 2892 181 209 1734
Fragments arranged by size:
2892 1734 209 181 128
BsrGI T'GTAC_A
Cuts at: 940 1132 1156 940
Size: 192 24 4928
Fragments arranged by size:
4928 192 24
BssSI C'ACGA_G
Cuts at: 3415 3773 3415
Size: 358 4786
BstAPI GCAn_nnn'nTGC
Cuts at: 565 779 1447 2521 565
Size: 214 668 1074 3188
Fragments arranged by size:
3188 1074 668 214
BstBI TT'CG_AA
Cuts at: 2070 3505 2070
Size: 1435 3709
BstXI CCAn_nnnn'nTGG
Cuts at: 1585 1585
Size: 5144
BstYI r'GATC_y
Cuts at: 1 222 300 693 1296 1476 2052 2169 2636
Size: 221 78 393 603 180 576 117 467
Cuts at: 2636 2659 2994 3240 4252 4338 4350 4696 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 603 576 467 449 393 346 335
246 221 180 117 86 78 23 12
BtgI C'CryG_G
Cuts at: 107 2135 107
Size: 2028 3116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3094 3275 3438 3094
Size: 181 163 4800
Fragments arranged by size:
4800 181 163
BtsI GCAGTG_nn'
Cuts at: 2387 2387
Size: 5144
DraI TTT'AAA
Cuts at: 1341 2143 1341
Size: 802 4342
DrdI GACnn_nn'nnGTC
Cuts at: 2850 3708 2850
Size: 858 4286
EaeI y'GGCC_r
Cuts at: 629 825 2729 2903 3294 3321 4666 629
Size: 196 1904 174 391 27 1345 1107
Fragments arranged by size:
1904 1345 1107 391 196 174 27
EagI C'GGCC_G
Cuts at: 2729 2729
Size: 5144
EarI CTCTTCn'nnn_
Cuts at: 1553 1959 2089 3167 3377 1553
Size: 406 130 1078 210 3320
Fragments arranged by size:
3320 1078 406 210 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 156 3352 3662 3808 4954 156
Size: 3196 310 146 1146 346
Fragments arranged by size:
3196 1146 346 310 146
EcoICRI GAG'CTC
Cuts at: 2156 2156
Size: 5144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1688 1814 2969 3401 4148 4416 1133
Size: 555 126 1155 432 747 268 1861
Fragments arranged by size:
1861 1155 747 555 432 268 126
EcoO109I rG'GnC_Cy
Cuts at: 2283 2283
Size: 5144
EcoRI G'AATT_C
Cuts at: 1091 2148 1091
Size: 1057 4087
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 5144
FspI TGC'GCA
Cuts at: 2925 2925
Size: 5144
HaeII r_GCGC'y
Cuts at: 880 2578 2826 3848 4827 880
Size: 1698 248 1022 979 1197
Fragments arranged by size:
1698 1197 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 248 270 280 302 1490 1522 1705 1737 1846
Size: 22 10 22 1188 32 183 32 109
Cuts at: 1846 1878 1921 1953 2986 3016 3018 3048 248
Size: 32 43 32 1033 30 2 30 2344
Fragments arranged by size:
2344 1188 1033 183 109 43 32 32
32 32 30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 440 1394 2183 440
Size: 954 789 3401
Fragments arranged by size:
3401 954 789
HindIII A'AGCT_T
Cuts at: 721 721
Size: 5144
HpaI GTT'AAC
Cuts at: 1394 1394
Size: 5144
KasI G'GCGC_C
Cuts at: 2574 2822 2574
Size: 248 4896
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 497 1839 3815 3999 497
Size: 1342 1976 184 1642
Fragments arranged by size:
1976 1642 1342 184
MscI TGG'CCA
Cuts at: 631 827 2905 631
Size: 196 2078 2870
Fragments arranged by size:
2870 2078 196
MslI CAynn'nnrTG
Cuts at: 904 967 1583 1768 1907 3133 3260 904
Size: 63 616 185 139 1226 127 2788
Fragments arranged by size:
2788 1226 616 185 139 127 63
MspA1I CmG'CkG
Cuts at: 219 511 1024 2255 2569 2929 3942 4187 219
Size: 292 513 1231 314 360 1013 245 1176
Fragments arranged by size:
1231 1176 1013 513 360 314 292 245
NaeI GCC'GGC
Cuts at: 3325 3325
Size: 5144
NarI GG'CG_CC
Cuts at: 2575 2823 2575
Size: 248 4896
NcoI C'CATG_G
Cuts at: 107 107
Size: 5144
NdeI CA'TA_TG
Cuts at: 480 2089 480
Size: 1609 3535
NgoMIV G'CCGG_C
Cuts at: 3323 3323
Size: 5144
NsiI A_TGCA'T
Cuts at: 1827 1827
Size: 5144
NspI r_CATG'y
Cuts at: 438 707 907 1286 1543 1878 1906 2197 2697
Size: 269 200 379 257 335 28 291 500
Cuts at: 2697 3604 438
Size: 907 1978
Fragments arranged by size:
1978 907 500 379 335 291 269 257
200 28
PciI A'CATG_T
Cuts at: 703 903 1539 1902 3600 703
Size: 200 636 363 1698 2247
Fragments arranged by size:
2247 1698 636 363 200
PflMI CCAn_nnn'nTGG
Cuts at: 2049 2049
Size: 5144
PfoI T'CCnGG_A
Cuts at: 1517 1517
Size: 5144
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 5112
PstI C_TGCA'G
Cuts at: 218 2191 2876 218
Size: 1973 685 2486
Fragments arranged by size:
2486 1973 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 219 511 1024 2569 2929 219
Size: 292 513 1545 360 2434
Fragments arranged by size:
2434 1545 513 360 292
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 2158 2158
Size: 5144
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 1959 2089 3167 3377 1959
Size: 130 1078 210 3726
Fragments arranged by size:
3726 1078 210 130
SbfI CC_TGCA'GG
Cuts at: 2191 2191
Size: 5144
ScaI AGT'ACT
Cuts at: 4661 4661
Size: 5144
SexAI A'CCwGG_T
Cuts at: 1169 2042 1169
Size: 873 4271
SfcI C'TryA_G
Cuts at: 33 214 1064 2187 2872 3865 4056 33
Size: 181 850 1123 685 993 191 1121
Fragments arranged by size:
1123 1121 993 850 685 191 181
SfoI GGC'GCC
Cuts at: 2576 2824 2576
Size: 248 4896
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SmaI CCC'GGG
Cuts at: 2166 2166
Size: 5144
SmlI C'TyrA_G
Cuts at: 1000 2299 3214 3706 3968 1000
Size: 1299 915 492 262 2176
Fragments arranged by size:
2176 1299 915 492 262
SnaBI TAC'GTA
Cuts at: 465 465
Size: 5144
SpeI A'CTAG_T
Cuts at: 67 498 67
Size: 431 4713
SphI G_CATG'C
Cuts at: 2197 2697 2197
Size: 500 4644
SspI AAT'ATT
Cuts at: 530 1525 530
Size: 995 4149
StuI AGG'CCT
Cuts at: 472 472
Size: 5144
StyI C'CwwG_G
Cuts at: 107 485 822 2278 2632 107
Size: 378 337 1456 354 2619
Fragments arranged by size:
2619 1456 378 354 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2727 2860 3319 3528 2727
Size: 133 459 209 4343
Fragments arranged by size:
4343 459 209 133
TatI w'GTAC_w
Cuts at: 188 351 940 1132 1156 4659 188
Size: 163 589 192 24 3503 673
Fragments arranged by size:
3503 673 589 192 163 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 4661 4693 2085
Size: 32 2544 32 2536
Fragments arranged by size:
2544 2536 32 32
Tth111I GACn'n_nGTC
Cuts at: 1719 2941 1719
Size: 1222 3922
XbaI T'CTAG_A
Cuts at: 2175 2175
Size: 5144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 624 639 1254 2639 624
Size: 15 615 1385 3129
Fragments arranged by size:
3129 1385 615 15
XmaI C'CCGG_G
Cuts at: 2164 2164
Size: 5144
XmnI GAAnn'nnTTC
Cuts at: 2083 2083
Size: 5144
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI
BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI MfeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI SwaI
XhoI ZraI