Restriction map for
pF1KA1430
(Linear) (Six-base) MAPSORT of: KIAA1430.seq Check: 1676 from: 1 to: 1596
KIAA1430.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AclI AA'CG_TT
Cuts at: 0 470 1368 1596
Size: 470 898 228
Fragments arranged by size:
898 470 228
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 764 781 956 1091 1596
Size: 527 237 17 175 135 505
Fragments arranged by size:
527 505 237 175 135 17
AflIII A'CryG_T
Cuts at: 0 336 338 627 1596
Size: 336 2 289 969
Fragments arranged by size:
969 336 289 2
AlwNI CAG_nnn'CTG
Cuts at: 0 577 1596
Size: 577 1019
ApoI r'AATT_y
Cuts at: 0 153 220 356 1596
Size: 153 67 136 1240
Fragments arranged by size:
1240 153 136 67
AvaI C'yCGr_G
Cuts at: 0 1117 1596
Size: 1117 479
BbsI GAAGACnn'nnnn_
Cuts at: 0 962 1566 1596
Size: 962 604 30
Fragments arranged by size:
962 604 30
BclI T'GATC_A
Cuts at: 0 825 1098 1245 1596
Size: 825 273 147 351
Fragments arranged by size:
825 351 273 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 820 1596
Size: 820 776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 969 1524 1596
Size: 764 205 555 72
Fragments arranged by size:
764 555 205 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1357 1596
Size: 1357 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 1507 1596
Size: 1507 89
BsaAI yAC'GTr
Cuts at: 0 339 1596
Size: 339 1257
BsaBI GATnn'nnATC
Cuts at: 0 584 1037 1596
Size: 584 453 559
Fragments arranged by size:
584 559 453
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 501 510 513 751 948 1596
Size: 501 9 3 238 197 648
Fragments arranged by size:
648 501 238 197 9 3
BsiEI CG_ry'CG
Cuts at: 0 1164 1596
Size: 1164 432
BsiHKAI G_wGCw'C
Cuts at: 0 1275 1596
Size: 1275 321
BsmI GAATG_Cn'
Cuts at: 0 173 1596
Size: 173 1423
BsmBI CGTCTCn'nnnn_
Cuts at: 0 605 1596
Size: 605 991
Bsp1286I G_dGCh'C
Cuts at: 0 1275 1596
Size: 1275 321
BsrDI GCAATG_nn'
Cuts at: 0 277 1596
Size: 277 1319
BstBI TT'CG_AA
Cuts at: 0 157 1596
Size: 157 1439
BstYI r'GATC_y
Cuts at: 0 1032 1441 1596
Size: 1032 409 155
Fragments arranged by size:
1032 409 155
Bsu36I CC'TnA_GG
Cuts at: 0 734 1596
Size: 734 862
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1378 1596
Size: 1378 218
BtsI GCAGTG_nn'
Cuts at: 0 420 1596
Size: 420 1176
ClaI AT'CG_AT
Cuts at: 0 1160 1596
Size: 1160 436
EaeI y'GGCC_r
Cuts at: 0 723 1452 1545 1596
Size: 723 729 93 51
Fragments arranged by size:
729 723 93 51
EarI CTCTTCn'nnn_
Cuts at: 0 294 519 800 1139 1596
Size: 294 225 281 339 457
Fragments arranged by size:
457 339 294 281 225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1219 1596
Size: 1219 377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 764 781 956 969 1091 1524 1596
Size: 527 237 17 175 13 122 433 72
Fragments arranged by size:
527 433 237 175 122 72 17 13
EcoRI G'AATT_C
Cuts at: 0 356 1596
Size: 356 1240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 943 975 1374 1406 1596
Size: 559 32 352 32 399 32 190
Fragments arranged by size:
559 399 352 190 32 32 32
HincII GTy'rAC
Cuts at: 0 1533 1596
Size: 1533 63
HindIII A'AGCT_T
Cuts at: 0 1052 1596
Size: 1052 544
MscI TGG'CCA
Cuts at: 0 725 1454 1547 1596
Size: 725 729 93 49
Fragments arranged by size:
729 725 93 49
MslI CAynn'nnrTG
Cuts at: 0 166 717 1596
Size: 166 551 879
Fragments arranged by size:
879 551 166
MspA1I CmG'CkG
Cuts at: 0 1528 1596
Size: 1528 68
NspI r_CATG'y
Cuts at: 0 631 1596
Size: 631 965
PciI A'CATG_T
Cuts at: 0 627 1596
Size: 627 969
PflMI CCAn_nnn'nTGG
Cuts at: 0 721 1357 1596
Size: 721 636 239
Fragments arranged by size:
721 636 239
PfoI T'CCnGG_A
Cuts at: 0 1477 1596
Size: 1477 119
PmlI CAC'GTG
Cuts at: 0 339 1596
Size: 339 1257
PshAI GACnn'nnGTC
Cuts at: 0 764 1596
Size: 764 832
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1353 1385 1596
Size: 1353 32 211
Fragments arranged by size:
1353 211 32
PvuI CG_AT'CG
Cuts at: 0 1164 1596
Size: 1164 432
ScaI AGT'ACT
Cuts at: 0 708 1596
Size: 708 888
SmaI CCC'GGG
Cuts at: 0 1119 1596
Size: 1119 477
SmlI C'TyrA_G
Cuts at: 0 1336 1596
Size: 1336 260
SspI AAT'ATT
Cuts at: 0 910 1596
Size: 910 686
TatI w'GTAC_w
Cuts at: 0 706 1225 1596
Size: 706 519 371
Fragments arranged by size:
706 519 371
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1473 1505 1596
Size: 1473 32 91
Fragments arranged by size:
1473 91 32
XmaI C'CCGG_G
Cuts at: 0 1117 1596
Size: 1117 479
Enzymes that do cut:
AclI AcuI AflIII AlwNI ApoI AvaI BbsI BclI
BmrI BpmI BpuEI BsaI BsaAI BsaBI BseRI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BsrDI BstBI BstYI Bsu36I
BtgZI BtsI ClaI EaeI EarI EciI Eco57MI EcoRI
Hin4I HincII HindIII MscI MslI MspA1I NspI PciI
PflMI PfoI PmlI PshAI PsrI PvuI ScaI SmaI
SmlI SspI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BsaHI BsaWI BsaXI BseYI BsgI BsiWI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstZ17I BtgI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PpiI PpuMI PsiI
PspOMI PspXI PstI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII Tth111I XbaI XcmI XhoI XmnI ZraI