Restriction map for pF1KA1386
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BanI        BbeI        
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MluI        
MroI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SwaI        XbaI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII       1716  ACACGT               A^C[GA][CT]GT
Alw44I        229  GTGCAC               G^TGCAC
ApaLI         229  GTGCAC               G^TGCAC
BalI         2877  TGGCCA               TGG^CCA
BamHI         615  GGATCC               G^GATCC
BbrPI        1717  CACGTG               CAC^GTG
BbvCI          82  CCTCAGC              CCTCAGC(-5/-2)
BcnI          828  CCCGG                CC^[GC]GG
BsaAI        1717  CACGTG               [CT]AC^GT[GA]
BsmI         1973  GCATTC               GAATGC(1/-1)
Bsp1407I     2148  TGTACA               T^GTACA
BsrDI        1346  CATTGC               GCAATG(2/0)
BsrGI        2148  TGTACA               T^GTACA
DraI         1813  TTTAAA               TTT^AAA
DraIII        875  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2318  GACAACGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2438  CCGTGG               C^C[GA][CT]GG
EaeI         2877  TGGCCA               [CT]^GGCC[GA]
EcoNI        1267  CCTACTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2167  GAATTC               G^AATTC
EcoT22I       247  ATGCAT               ATGCA^T
HapII         829  CCGG                 C^CGG
HpaII         829  CCGG                 C^CGG
MluNI        2877  TGGCCA               TGG^CCA
MscI         2877  TGGCCA               TGG^CCA
MspA1I       2873  CAGCTG               C[AC]G^C[GT]G
MspI          829  CCGG                 C^CGG
NciI          828  CCCGG                CC^[GC]GG
NsiI          247  ATGCAT               ATGCA^T
PmaCI        1717  CACGTG               CAC^GTG
PmlI         1717  CACGTG               CAC^GTG
PvuII        2873  CAGCTG               CAG^CTG
SphI         2140  GCATGC               GCATG^C
SspBI        2148  TGTACA               T^GTACA
XcmI         1865  CCAAAGAAAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          701  GTAGAC               GT^[AC][GT]AC
             2345  GTCTAC              
AlwNI        2363  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2530  CAGGCTCTG           
Asp700I      1705  GAAGTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3130  GAAAATATTC          
AspI         2318  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2689  GACTCTGTC           
AvaII         265  GGACC                G^G[AT]CC
             2883  GGACC               
BsaBI         205  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2267  GATTTGCATC          
CfoI         1971  GCGC                 GCG^C
             2226  GCGC                
DraII         264  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2882  AGGACCC             
EcoO109I      264  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2882  AGGACCC             
EcoRV         279  GATATC               GAT^ATC
             1168  GATATC              
FauI         2117  CCCGC                CCCGC(4/6)
             3142  GCGGG               
HhaI         1971  GCGC                 GCG^C
             2226  GCGC                
HinP1I       1971  GCGC                 G^CGC
             2226  GCGC                
HindIII       767  AAGCTT               A^AGCTT
              812  AAGCTT              
MaeII        1718  ACGT                 A^CGT
             2322  ACGT                
MamI          205  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2267  GATTTGCATC          
MfeI         1521  CAATTG               C^AATTG
             1882  CAATTG              
MslI         1002  CATTTGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2433  CACATCCGTG          
MunI         1521  CAATTG               C^AATTG
             1882  CAATTG              
PflFI        2318  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2689  GACTCTGTC           
PflMI         874  CCACCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1253  CCATTAAATGG         
PpuMI         264  GGGACCT              [GA]G^G[AT]CC[CT]
             2882  AGGACCC             
Tth111I      2318  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2689  GACTCTGTC           
Van91I        874  CCACCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1253  CCATTAAATGG         
XmnI         1705  GAAGTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3130  GAAAATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        AspHI       AvaI        BanII       
BbsI        BbvI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspHI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       EarI        Eco81I      EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MvaI        MwoI        NdeI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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