Restriction map for pF1KA1386
 (Linear) (Six-base) MAPSORT of: KIAA1386.seq  Check: 2125  from: 1  to: 3267



KIAA1386.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:22  ..

AccI GT'mk_AC

Cuts at:      0     702    2346    3267 
   Size:        702    1644     921

  Fragments arranged by size: 

                1644     921     702
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     647     727     785    1928    2093    2512    3267 
   Size:        305     342      80      58    1143     165     419     755

  Fragments arranged by size: 

                1143     755     419     342     305     165      80      58
            
 
AflIII A'CryG_T

Cuts at:      0    1716    3267 
   Size:       1716    1551
 
AleI CACnn'nnGTG

Cuts at:      0    2437    3267 
   Size:       2437     830
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     470    1161    1618    3267 
   Size:        470     691     457    1649

  Fragments arranged by size: 

                1649     691     470     457
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2368    2535    3267 
   Size:       2368     167     732

  Fragments arranged by size: 

                2368     732     167
 
ApaLI G'TGCA_C

Cuts at:      0     229    3267 
   Size:        229    3038
 
ApoI r'AATT_y

Cuts at:      0      19    1100    2033    2167    2293    3267 
   Size:         19    1081     933     134     126     974

  Fragments arranged by size: 

                1081     974     933     134     126      19
 
AvaI C'yCGr_G

Cuts at:      0     619    2186    2717    3267 
   Size:        619    1567     531     550

  Fragments arranged by size: 

                1567     619     550     531
 
BamHI G'GATC_C

Cuts at:      0     615    3267 
   Size:        615    2652
 
BanII G_rGCy'C

Cuts at:      0     128     294     783    1359    1896    2815    3267 
   Size:        128     166     489     576     537     919     452

  Fragments arranged by size: 

                 919     576     537     489     452     166     128
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1037    2081    2558    3267 
   Size:       1037    1044     477     709

  Fragments arranged by size: 

                1044    1037     709     477
 
BbvCI CC'TCA_GC

Cuts at:      0      83    3267 
   Size:         83    3184
 
BfrBI ATG'CAT

Cuts at:      0     249    3267 
   Size:        249    3018
 
Bme1580I G_kGCm'C

Cuts at:      0     233    3267 
   Size:        233    3034
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     798    3267 
   Size:        798    2469
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1910    2650    3267 
   Size:       1910     740     617

  Fragments arranged by size: 

                1910     740     617
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1256    1697    2345    2951    3267 
   Size:       1256     441     648     606     316

  Fragments arranged by size: 

                1256     648     606     441     316
 
Bpu10I CC'TnA_GC

Cuts at:      0      83    2809    3267 
   Size:         83    2726     458

  Fragments arranged by size: 

                2726     458      83
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1963    3143    3219    3267 
   Size:       1963    1180      76      48

  Fragments arranged by size: 

                1963    1180      76      48
 
BsaAI yAC'GTr

Cuts at:      0    1719    3267 
   Size:       1719    1548
 
BsaBI GATnn'nnATC

Cuts at:      0     209    2271    3267 
   Size:        209    2062     996

  Fragments arranged by size: 

                2062     996     209
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     518     740    1738    2322    2345    3209    3267 
   Size:        518     222     998     584      23     864      58

  Fragments arranged by size: 

                 998     864     584     518     222      58      23
 
BseYI C'CCAG_C

Cuts at:      0    1394    2996    3267 
   Size:       1394    1602     271

  Fragments arranged by size: 

                1602    1394     271
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1032    2452    3008    3267 
   Size:       1032    1420     556     259

  Fragments arranged by size: 

                1420    1032     556     259
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     783    1359    1896    1958    2815    3267 
   Size:        233     550     576     537      62     857     452

  Fragments arranged by size: 

                 857     576     550     537     452     233      62
 
BsmI GAATG_Cn'

Cuts at:      0    1973    3267 
   Size:       1973    1294
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     233     294     783    1359    1896    1958    2815 
   Size:        128     105      61     489     576     537      62     857

Cuts at:   2815    3267 
   Size:        452

  Fragments arranged by size: 

                 857     576     537     489     452     128     105      62
                  61
 
BspHI T'CATG_A

Cuts at:      0     783    2765    2931    3267 
   Size:        783    1982     166     336

  Fragments arranged by size: 

                1982     783     336     166
 
BsrDI GCAATG_nn'

Cuts at:      0    1345    3267 
   Size:       1345    1922
 
BsrGI T'GTAC_A

Cuts at:      0    2148    3267 
   Size:       2148    1119
 
BstYI r'GATC_y

Cuts at:      0     615     693    1324    3267 
   Size:        615      78     631    1943

  Fragments arranged by size: 

                1943     631     615      78
 
Bsu36I CC'TnA_GG

Cuts at:      0     462     689    2652    3267 
   Size:        462     227    1963     615

  Fragments arranged by size: 

                1963     615     462     227
 
BtgI C'CryG_G

Cuts at:      0    2438    3267 
   Size:       2438     829
 
BtsI GCAGTG_nn'

Cuts at:      0    1612    2587    3267 
   Size:       1612     975     680

  Fragments arranged by size: 

                1612     975     680
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1917    1952    3267 
   Size:       1917      35    1315

  Fragments arranged by size: 

                1917    1315      35
 
DraI TTT'AAA

Cuts at:      0    1815    3267 
   Size:       1815    1452
 
DraIII CAC_nnn'GTG

Cuts at:      0     880    3267 
   Size:        880    2387
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2324    3267 
   Size:       2324     943
 
EaeI y'GGCC_r

Cuts at:      0    2877    3267 
   Size:       2877     390
 
EarI CTCTTCn'nnn_

Cuts at:      0     330     942    1393    1643    2152    2481    2531    3267 
   Size:        330     612     451     250     509     329      50     736

  Fragments arranged by size: 

                 736     612     509     451     330     329     250      50
            
 
EcoICRI GAG'CTC

Cuts at:      0     781    1357    1894    2813    3267 
   Size:        781     576     537     919     454

  Fragments arranged by size: 

                 919     781     576     537     454
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     305     647     727     785    1256    1697    1928    2093 
   Size:        305     342      80      58     471     441     231     165

Cuts at:   2093    2345    2512    2951    3267 
   Size:        252     167     439     316

  Fragments arranged by size: 

                 471     441     439     342     316     305     252     231
                 167     165      80      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1271    3267 
   Size:       1271    1996
 
EcoO109I rG'GnC_Cy

Cuts at:      0     265    2883    3267 
   Size:        265    2618     384

  Fragments arranged by size: 

                2618     384     265
 
EcoRI G'AATT_C

Cuts at:      0    2167    3267 
   Size:       2167    1100
 
EcoRV GAT'ATC

Cuts at:      0     281    1170    3267 
   Size:        281     889    2097

  Fragments arranged by size: 

                2097     889     281
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     115     310     733     790    1644    3267 
   Size:        115     195     423      57     854    1623

  Fragments arranged by size: 

                1623     854     423     195     115      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1714    1746    2854    2886    3267 
   Size:       1714      32    1108      32     381

  Fragments arranged by size: 

                1714    1108     381      32      32
 
HindIII A'AGCT_T

Cuts at:      0     767     812    3267 
   Size:        767      45    2455

  Fragments arranged by size: 

                2455     767      45
 
MfeI C'AATT_G

Cuts at:      0    1521    1882    3267 
   Size:       1521     361    1385

  Fragments arranged by size: 

                1521    1385     361
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     848    1176    3267 
   Size:        848     328    2091

  Fragments arranged by size: 

                2091     848     328
 
MscI TGG'CCA

Cuts at:      0    2879    3267 
   Size:       2879     388
 
MslI CAynn'nnrTG

Cuts at:      0    1006    2437    3267 
   Size:       1006    1431     830

  Fragments arranged by size: 

                1431    1006     830
 
MspA1I CmG'CkG

Cuts at:      0    2875    3267 
   Size:       2875     392
 
NdeI CA'TA_TG

Cuts at:      0     672    1140    1995    3267 
   Size:        672     468     855    1272

  Fragments arranged by size: 

                1272     855     672     468
 
NsiI A_TGCA'T

Cuts at:      0     251    3267 
   Size:        251    3016
 
NspI r_CATG'y

Cuts at:      0     741    1605    2144    3267 
   Size:        741     864     539    1123

  Fragments arranged by size: 

                1123     864     741     539
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     880    1259    3267 
   Size:        880     379    2008

  Fragments arranged by size: 

                2008     880     379
 
PmlI CAC'GTG

Cuts at:      0    1719    3267 
   Size:       1719    1548
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2321    2353    3267 
   Size:       2321      32     914

  Fragments arranged by size: 

                2321     914      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     265    2883    3267 
   Size:        265    2618     384

  Fragments arranged by size: 

                2618     384     265
 
PstI C_TGCA'G

Cuts at:      0     353     735    2205    3267 
   Size:        353     382    1470    1062

  Fragments arranged by size: 

                1470    1062     382     353
 
PvuII CAG'CTG

Cuts at:      0    2875    3267 
   Size:       2875     392
 
SacI G_AGCT'C

Cuts at:      0     783    1359    1896    2815    3267 
   Size:        783     576     537     919     452

  Fragments arranged by size: 

                 919     783     576     537     452
 
SapI GCTCTTCn'nnn_

Cuts at:      0     330     942    2481    3267 
   Size:        330     612    1539     786

  Fragments arranged by size: 

                1539     786     612     330
 
SfcI C'TryA_G

Cuts at:      0     349     731    1213    2201    2975    3267 
   Size:        349     382     482     988     774     292

  Fragments arranged by size: 

                 988     774     482     382     349     292
 
SmlI C'TyrA_G

Cuts at:      0    1978    3158    3234    3267 
   Size:       1978    1180      76      33

  Fragments arranged by size: 

                1978    1180      76      33
 
SphI G_CATG'C

Cuts at:      0    2144    3267 
   Size:       2144    1123
 
SspI AAT'ATT

Cuts at:      0     182    2260    3135    3267 
   Size:        182    2078     875     132

  Fragments arranged by size: 

                2078     875     182     132
 
StuI AGG'CCT

Cuts at:      0     687    1231    2100    2427    3267 
   Size:        687     544     869     327     840

  Fragments arranged by size: 

                 869     840     687     544     327
 
StyI C'CwwG_G

Cuts at:      0     922    1404    1733    2221    2543    3267 
   Size:        922     482     329     488     322     724

  Fragments arranged by size: 

                 922     724     488     482     329     322
 
TatI w'GTAC_w

Cuts at:      0    1693    2148    3255    3267 
   Size:       1693     455    1107      12

  Fragments arranged by size: 

                1693    1107     455      12
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1325    1357    2461    2493    3267 
   Size:       1325      32    1104      32     774

  Fragments arranged by size: 

                1325    1104     774      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2321    2692    3267 
   Size:       2321     371     575

  Fragments arranged by size: 

                2321     575     371
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1872    3267 
   Size:       1872    1395
 
XmnI GAAnn'nnTTC

Cuts at:      0    1709    3134    3267 
   Size:       1709    1425     133

  Fragments arranged by size: 

                1709    1425     133
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI     ApoI
     AvaI    BamHI    BanII     BbsI    BbvCI    BfrBI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI    BsrGI    BstYI
   Bsu36I     BtgI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
    Hin4I  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NdeI
     NsiI     NspI    PflMI     PmlI     PpiI    PpuMI     PstI    PvuII
     SacI     SapI     SfcI     SmlI     SphI     SspI     StuI     StyI
     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BanI
     BbeI     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI
     BmtI     BsaI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI     EagI     EciI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MluI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI     PfoI     PmeI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SwaI    TaqII     XbaI     XhoI     XmaI     ZraI
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