Restriction map for
pF1KA1351
(Linear) (Six-base) MAPSORT of: KIAA1351.seq Check: 6786 from: 1 to: 3672
KIAA1351.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 915 3672
Size: 915 2757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1059 2342 3672
Size: 727 332 1283 1330
Fragments arranged by size:
1330 1283 727 332
AflIII A'CryG_T
Cuts at: 0 776 942 3672
Size: 776 166 2730
Fragments arranged by size:
2730 776 166
AleI CACnn'nnGTG
Cuts at: 0 1544 3672
Size: 1544 2128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3252 3672
Size: 3252 420
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1176 1208 3672
Size: 1176 32 2464
Fragments arranged by size:
2464 1176 32
AlwNI CAG_nnn'CTG
Cuts at: 0 589 1458 3012 3048 3672
Size: 589 869 1554 36 624
Fragments arranged by size:
1554 869 624 589 36
ApaI G_GGCC'C
Cuts at: 0 54 3672
Size: 54 3618
ApaLI G'TGCA_C
Cuts at: 0 1348 3672
Size: 1348 2324
ApoI r'AATT_y
Cuts at: 0 686 718 1176 1235 2190 2196 2673 2716
Size: 686 32 458 59 955 6 477 43
Cuts at: 2716 2932 3672
Size: 216 740
Fragments arranged by size:
955 740 686 477 458 216 59 43
32 6
AseI AT'TA_AT
Cuts at: 0 2296 3672
Size: 2296 1376
AvaI C'yCGr_G
Cuts at: 0 794 1901 2024 2864 3672
Size: 794 1107 123 840 808
Fragments arranged by size:
1107 840 808 794 123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2153 2186 3672
Size: 2153 33 1486
Fragments arranged by size:
2153 1486 33
BanI G'GyrC_C
Cuts at: 0 445 658 2525 2735 3672
Size: 445 213 1867 210 937
Fragments arranged by size:
1867 937 445 213 210
BanII G_rGCy'C
Cuts at: 0 54 3672
Size: 54 3618
BciVI GTATCCnnnnn_n'
Cuts at: 0 2232 2325 3672
Size: 2232 93 1347
Fragments arranged by size:
2232 1347 93
BclI T'GATC_A
Cuts at: 0 1967 2982 3672
Size: 1967 1015 690
Fragments arranged by size:
1967 1015 690
BglII A'GATC_T
Cuts at: 0 952 3672
Size: 952 2720
Bme1580I G_kGCm'C
Cuts at: 0 54 1352 2530 3672
Size: 54 1298 1178 1142
Fragments arranged by size:
1298 1178 1142 54
BmgBI CAC'GTC
Cuts at: 0 2904 3672
Size: 2904 768
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 3672
Size: 93 3579
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3607 3672
Size: 3607 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 3672
Size: 1795 1877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 838 1138 1339 3466 3661 3672
Size: 683 155 300 201 2127 195 11
Fragments arranged by size:
2127 683 300 201 195 155 11
BsaAI yAC'GTr
Cuts at: 0 943 3672
Size: 943 2729
BsaBI GATnn'nnATC
Cuts at: 0 805 998 3672
Size: 805 193 2674
Fragments arranged by size:
2674 805 193
BsaHI Gr'CG_yC
Cuts at: 0 3233 3672
Size: 3233 439
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2309 2339 2829 2859 3672
Size: 2309 30 490 30 813
Fragments arranged by size:
2309 813 490 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2647 2852 3322 3382 3672
Size: 2647 205 470 60 290
Fragments arranged by size:
2647 470 290 205 60
BseYI C'CCAG_C
Cuts at: 0 627 1309 3672
Size: 627 682 2363
Fragments arranged by size:
2363 682 627
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2761 2791 3061 3083 3672
Size: 2761 30 270 22 589
Fragments arranged by size:
2761 589 270 30 22
BsiHKAI G_wGCw'C
Cuts at: 0 428 1352 2106 2909 3145 3672
Size: 428 924 754 803 236 527
Fragments arranged by size:
924 803 754 527 428 236
BsmI GAATG_Cn'
Cuts at: 0 438 1110 3672
Size: 438 672 2562
Fragments arranged by size:
2562 672 438
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3418 3672
Size: 3418 254
Bsp1286I G_dGCh'C
Cuts at: 0 54 428 1352 2106 2530 2909 3145 3672
Size: 54 374 924 754 424 379 236 527
Fragments arranged by size:
924 754 527 424 379 374 236 54
BspHI T'CATG_A
Cuts at: 0 1139 3672
Size: 1139 2533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 764 2149 2813 3254 3672
Size: 764 1385 664 441 418
Fragments arranged by size:
1385 764 664 441 418
BsrDI GCAATG_nn'
Cuts at: 0 1670 1701 2309 3672
Size: 1670 31 608 1363
Fragments arranged by size:
1670 1363 608 31
BsrGI T'GTAC_A
Cuts at: 0 2306 2998 3672
Size: 2306 692 674
Fragments arranged by size:
2306 692 674
BssHII G'CGCG_C
Cuts at: 0 34 3672
Size: 34 3638
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3547 3672
Size: 3547 125
BstBI TT'CG_AA
Cuts at: 0 948 3672
Size: 948 2724
BstEII G'GTnAC_C
Cuts at: 0 1853 3672
Size: 1853 1819
BstXI CCAn_nnnn'nTGG
Cuts at: 0 232 3153 3672
Size: 232 2921 519
Fragments arranged by size:
2921 519 232
BstYI r'GATC_y
Cuts at: 0 323 952 993 999 3672
Size: 323 629 41 6 2673
Fragments arranged by size:
2673 629 323 41 6
Bsu36I CC'TnA_GG
Cuts at: 0 582 3305 3672
Size: 582 2723 367
Fragments arranged by size:
2723 582 367
BtgI C'CryG_G
Cuts at: 0 1910 3672
Size: 1910 1762
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2130 3672
Size: 2130 1542
BtsI GCAGTG_nn'
Cuts at: 0 1456 3527 3672
Size: 1456 2071 145
Fragments arranged by size:
2071 1456 145
DraI TTT'AAA
Cuts at: 0 2195 3672
Size: 2195 1477
EaeI y'GGCC_r
Cuts at: 0 644 2055 3672
Size: 644 1411 1617
Fragments arranged by size:
1617 1411 644
EarI CTCTTCn'nnn_
Cuts at: 0 164 457 1867 3662 3672
Size: 164 293 1410 1795 10
Fragments arranged by size:
1795 1410 293 164 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1059 1795 2342 3672
Size: 727 332 736 547 1330
Fragments arranged by size:
1330 736 727 547 332
EcoNI CCTnn'n_nnAGG
Cuts at: 0 581 2279 3672
Size: 581 1698 1393
Fragments arranged by size:
1698 1393 581
EcoO109I rG'GnC_Cy
Cuts at: 0 50 51 586 2549 3130 3672
Size: 50 1 535 1963 581 542
Fragments arranged by size:
1963 581 542 535 50 1
EcoRI G'AATT_C
Cuts at: 0 718 1235 2673 2716 3672
Size: 718 517 1438 43 956
Fragments arranged by size:
1438 956 718 517 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3628 3660 3672
Size: 3628 32 12
Fragments arranged by size:
3628 32 12
HincII GTy'rAC
Cuts at: 0 1427 2510 2625 2975 3339 3672
Size: 1427 1083 115 350 364 333
Fragments arranged by size:
1427 1083 364 350 333 115
HindIII A'AGCT_T
Cuts at: 0 3473 3672
Size: 3473 199
HpaI GTT'AAC
Cuts at: 0 2510 3672
Size: 2510 1162
MfeI C'AATT_G
Cuts at: 0 1304 3672
Size: 1304 2368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1681 3032 3672
Size: 1681 1351 640
Fragments arranged by size:
1681 1351 640
MscI TGG'CCA
Cuts at: 0 646 2057 3672
Size: 646 1411 1615
Fragments arranged by size:
1615 1411 646
MslI CAynn'nnrTG
Cuts at: 0 923 1444 1544 1714 3672
Size: 923 521 100 170 1958
Fragments arranged by size:
1958 923 521 170 100
MspA1I CmG'CkG
Cuts at: 0 3616 3672
Size: 3616 56
NcoI C'CATG_G
Cuts at: 0 1910 3672
Size: 1910 1762
NspI r_CATG'y
Cuts at: 0 337 780 3672
Size: 337 443 2892
Fragments arranged by size:
2892 443 337
PasI CC'CwG_GG
Cuts at: 0 589 3672
Size: 589 3083
PciI A'CATG_T
Cuts at: 0 776 3672
Size: 776 2896
PflMI CCAn_nnn'nTGG
Cuts at: 0 455 2119 3672
Size: 455 1664 1553
Fragments arranged by size:
1664 1553 455
PfoI T'CCnGG_A
Cuts at: 0 347 3672
Size: 347 3325
PshAI GACnn'nnGTC
Cuts at: 0 1927 3672
Size: 1927 1745
PsiI TTA'TAA
Cuts at: 0 959 3672
Size: 959 2713
PspOMI G'GGCC_C
Cuts at: 0 50 3672
Size: 50 3622
PstI C_TGCA'G
Cuts at: 0 1164 1982 3231 3251 3672
Size: 1164 818 1249 20 421
Fragments arranged by size:
1249 1164 818 421 20
PvuII CAG'CTG
Cuts at: 0 3616 3672
Size: 3616 56
SapI GCTCTTCn'nnn_
Cuts at: 0 457 1867 3672
Size: 457 1410 1805
Fragments arranged by size:
1805 1410 457
SfcI C'TryA_G
Cuts at: 0 1160 1241 1252 1978 2956 3227 3247 3672
Size: 1160 81 11 726 978 271 20 425
Fragments arranged by size:
1160 978 726 425 271 81 20 11
SmaI CCC'GGG
Cuts at: 0 2026 3672
Size: 2026 1646
SmlI C'TyrA_G
Cuts at: 0 698 794 817 1153 1354 1901 2864 3481
Size: 698 96 23 336 201 547 963 617
Cuts at: 3481 3640 3672
Size: 159 32
Fragments arranged by size:
963 698 617 547 336 201 159 96
32 23
SpeI A'CTAG_T
Cuts at: 0 1579 3672
Size: 1579 2093
SphI G_CATG'C
Cuts at: 0 337 3672
Size: 337 3335
StyI C'CwwG_G
Cuts at: 0 1910 2545 2587 3650 3672
Size: 1910 635 42 1063 22
Fragments arranged by size:
1910 1063 635 42 22
SwaI ATTT'AAAT
Cuts at: 0 2195 3672
Size: 2195 1477
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 2241 2435 3672
Size: 826 1415 194 1237
Fragments arranged by size:
1415 1237 826 194
TatI w'GTAC_w
Cuts at: 0 2306 2998 3672
Size: 2306 692 674
Fragments arranged by size:
2306 692 674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 849 881 2309 2341 2800 2829
Size: 343 32 474 32 1428 32 459 29
Cuts at: 2829 2832 2861 3672
Size: 3 29 811
Fragments arranged by size:
1428 811 474 459 343 32 32 32
29 29 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1507 2590 3153 3183 3672
Size: 1507 1083 563 30 489
Fragments arranged by size:
1507 1083 563 489 30
XhoI C'TCGA_G
Cuts at: 0 794 1901 2864 3672
Size: 794 1107 963 808
Fragments arranged by size:
1107 963 808 794
XmaI C'CCGG_G
Cuts at: 0 2024 3672
Size: 2024 1648
XmnI GAAnn'nnTTC
Cuts at: 0 2569 3672
Size: 2569 1103
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AvaI BaeI BanI BanII BciVI
BclI BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NcoI NspI PasI PciI PflMI PfoI PshAI
PsiI PspOMI PstI PvuII SapI SfcI SmaI SmlI
SpeI SphI StyI SwaI TaqII TatI TstI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AscI AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI
BfrBI BglI BlpI BmtI Bpu10I BsaI BsaWI BsiEI
BsiWI BspEI BsrBI BsrFI BssSI BstZ17I ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PpuMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SrfI SspI StuI Tth111I XbaI
ZraI