Restriction map for pF1KA1221
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AgeI        
AhdI        Aor51HI     ApaI        AscI        Asp700I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BanII       BbeI        BbrPI       BcgI        
BciVI       BcnI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
FauI        FseI        FspI        HaeII       HapII       
HhaI        HinP1I      HpaI        HpaII       KasI        
KspI        MluI        MluNI       MroI        MscI        
MspI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       1510  GGTACC               G^GTACC
AcyI         2377  GACGCC               G[GA]^CG[CT]C
AflII         258  CTTAAG               C^TTAAG
Alw44I       1478  GTGCAC               G^TGCAC
AlwNI        3413  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          734  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1478  GTGCAC               G^TGCAC
AseI         2644  ATTAAT               AT^TAAT
AsnI         2644  ATTAAT               AT^TAAT
Asp718I      1510  GGTACC               G^GTACC
BamHI         777  GGATCC               G^GATCC
BanI         1510  GGTACC               G^G[CT][GA]CC
BbvCI        2806  CCTCAGC              CCTCAGC(-5/-2)
BclI         2246  TGATCA               T^GATCA
BfrI          258  CTTAAG               C^TTAAG
BglI         1625  GCCATCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3721  AGATCT               A^GATCT
BsaHI        2377  GACGCC               G[GA]^CG[CT]C
BsiYI        3880  CCACGGACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         3880  CCACGGACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI        1330  GTCCC                GGGAC(10/14)
Bsp1407I     1234  TGTACA               T^GTACA
BspHI        1712  TCATGA               T^CATGA
BspLU11I     2090  ACATGT               A^CATGT
BsrBI         159  GAGCGG               CCGCTC(-3/-3)
BsrGI        1234  TGTACA               T^GTACA
Bsu36I        734  CCTCAGG              CC^T[ACGT]AGG
DraII        3813  AGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI         2865  GACTGATTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         3880  CCACGG               C^C[GA][CT]GG
Eco81I        734  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     3813  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT22I      1019  ATGCAT               ATGCA^T
FbaI         2246  TGATCA               T^GATCA
HgaI         2377  GACGC                GACGC(5/10)
Hin1I        2377  GACGCC               G[GA]^CG[CT]C
HindIII      2394  AAGCTT               A^AGCTT
KpnI         1510  GGTACC               GGTAC^C
MfeI         1515  CAATTG               C^AATTG
MunI         1515  CAATTG               C^AATTG
NsiI         1019  ATGCAT               ATGCA^T
PflMI        3880  CCACGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        3813  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        2644  ATTAAT               AT^TAAT
PstI         2822  CTGCAG               CTGCA^G
RcaI         1712  TCATGA               T^CATGA
ScaI         1817  AGTACT               AGT^ACT
SspBI        1234  TGTACA               T^GTACA
StuI         2386  AGGCCT               AGG^CCT
Van91I       3880  CCACGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AciI          161  GCGG                 CCGC(-3/-1)
             2037  GCGG                
AflIII       2090  ACATGT               A^C[GA][CT]GT
             2412  ACGTGT              
AspHI         296  GTGCTC               G[AT]GC[AT]^C
             1478  GTGCAC              
AvaII         352  GGACC                G^G[AT]CC
             3814  GGACC               
BmyI          296  GTGCTC               G[AGT]GC[ACT]^C
             1478  GTGCAC              
BpmI         2705  CTGGAG               CTGGAG(16/14)
             3887  CTGGAG              
BsaBI        2479  GATAGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2641  GATATTAATC          
BsiHKAI       296  GTGCTC               G[AT]GC[AT]^C
             1478  GTGCAC              
BsmI         1621  GAATGC               GAATGC(1/-1)
             1824  GCATTC              
Bsp1286I      296  GTGCTC               G[AGT]GC[ACT]^C
             1478  GTGCAC              
BspMI        1194  ACCTGC               ACCTGC(4/8)
             3788  GCAGGT              
BstXI          68  CCAACAATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2733  CCAAAACGATGG        
Cfr13I        352  GGACC                G^G[ACGT]CC
             3814  GGACC               
DraI         1800  TTTAAA               TTT^AAA
             1968  TTTAAA              
EaeI         2038  CGGCCA               [CT]^GGCC[GA]
             3965  TGGCCG              
EcoRI         615  GAATTC               G^AATTC
             3552  GAATTC              
EcoT14I      1333  CCAAGG               C^C[AT][AT]GG
             2195  CCAAGG              
MamI         2479  GATAGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2641  GATATTAATC          
MspA1I        473  CAGCTG               C[AC]G^C[GT]G
              484  CAGCTG              
PshAI        2508  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3815  GACCCTTGTC          
PvuII         473  CAGCTG               CAG^CTG
              484  CAGCTG              
SapI          988  GAAGAGC              GCTCTTC(1/4)
             1213  GAAGAGC             
Sau96I        352  GGACC                G^G[ACGT]CC
             3814  GGACC               
SmlI          258  CTTAAG               C^T[CT][GA]AG
             2878  CTTGAG              
StyI         1333  CCAAGG               C^C[AT][AT]GG
             2195  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccI        AcsI        AfaI        AluI        AlwI        
ApoI        BbsI        BbvI        BfaI        BpuAI       
BsaJI       BseRI       BsmAI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HaeIII      HincII      HindII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MvaI        MwoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        Sau3AI      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XbaI        XhoII       
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